Aqueous two-phase systems for protein separation: a perspective.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Journal of Chromatography A

• Publication date

  2011-12-09

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.chroma.2011.06.051PMID 21752387
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Theoretical and Experimental Evaluation of Hydrophobicity of Proteins to Predict their Partitioning Behavior in Aqueous Two Phase Systems: A Review

  2010cited by this paper
• Purification of lipase derived from Burkholderia pseudomallei with alcohol/salt-based aqueous two-phase systems

  2009cited by this paper
• Application of aqueous two-phase systems to antibody purification: a multi-stage approach.

  2009cited by this paper
• Aqueous two-phase extraction (ATPE): An attractive and economically viable technology for downstream processing of Aspergillus oryzae α-galactosidase

  2008cited by this paper
• Affinity partitioning of plasmid DNA with a zinc finger protein.

  2008cited by this paper
• Challenges and trends in bioseparations

  2008cited by this paper
• Prediction of the partitioning behaviour of proteins in aqueous two-phase systems using only their amino acid composition.

  2008cited by this paper
• Partition features and renaturation enhancement of chymosin in aqueous two-phase systems.

  2007cited by this paper
• Reverse micellar extraction systems for the purification of pharmaceutical grade plasmid DNA.

  2007cited by this paper
• Predicting the behaviour of proteins in hydrophobic interaction chromatography. 2. Using a statistical description of their surface amino acid distribution.

  2006cited by this paper
• Rotavirus-like particles primary recovery from insect cells in aqueous two-phase systems.

  2006cited by this paper
• Predicting the behaviour of proteins in hydrophobic interaction chromatography. 1: Using the hydrophobic imbalance (HI) to describe their surface amino acid distribution.

  2006cited by this paper
• Correlation for the partition behavior of proteins in aqueous two-phase systems: effect of surface hydrophobicity and charge.

  2005cited by this paper
• Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography.

  2005cited by this paper
• Practical application of aqueous two-phase partition to process development for the recovery of biological products.

  2004cited by this paper
• Effect of electrostatic energy on partitioning of proteins in aqueous two-phase systems.

  2004cited by this paper
• Phase separation rates of aqueous two‐phase systems: Correlation with system properties

  2002cited by this paper
• The surface exposed amino acid residues of monomeric proteins determine the partitioning in aqueous two-phase systems.

  2002cited by this paper
• Amylase partitioning and extractive bioconversion of starch using thermoseparating aqueous two-phase systems.

  2002cited by this paper
• Use of chemically modified proteins to study the effect of a single protein property on partitioning in aqueous two‐phase systems: Effect of surface hydrophobicity

  2000cited by this paper
• Aqueous two-phase systems

  2000cited by this paper
• Conservative chemical modification of proteins to study the effects of a single protein property on partitioning in aqueous two‐phase systems

  2000cited by this paper
• Optimization and design considerations of two‐phase continuous protein separation

  1999cited by this paper
• Aqueous two-phase systems for protein separation. Studies on phase inversion.

  1998cited by this paper
• Separation of proteins and viruses using two-phase aqueous micellar systems.

  1998cited by this paper
• On the kinetics of phase separation in aqueous two-phase systems.

  1998cited by this paper
• The application of aqueous two-phase systems to the purification of pharmaceutical proteins from transgenic sheep milk

  1997cited by this paper
• Reverse micellar mass‐transfer processes: Spray column extraction of lysozyme

  1996cited by this paper
• Use of chemically modified proteins to study the effect of a single protein property on partitioning in aqueous two-phase systems: Effect of surface charge.

  1996cited by this paper
• Studies on the purification of peroxidase from horseradish roots using reverse micelles.

  1996cited by this paper
• Correlations for the partition behavior of proteins in aqueous two‐phase systems: Effect of overall protein concentration

  1996cited by this paper
• Protein partitioning equilibrium between the aqueous poly(ethylene glycol) and salt phases and the solid protein phase in poly(ethylene glycol)-salt two-phase systems.

  1996cited by this paper
• Partitioning and purification of monoclonal antibodies in aqueous two-phase systems.

  1996cited by this paper
• Theory of phase formation in aqueous two-phase systems.

  1996cited by this paper
• Use of aqueous two-phase systems in the purification of human interferon-α1 from recombinant Escherichia coli

  1996cited by this paper
• Mathematical modelling and computer simulation of aqueous two-phase continuous protein extraction☆

  1996cited by this paper
• Extraction of lysozyme and ribonuclease‐a using reverse micelles: Limits to protein solubilization

  1995cited by this paper
• Purification of virus like particles from yeast cells using aqueous two-phase systems.

  1995cited by this paper
• The Mobile Robot RHINO

  1995cited by this paper
• Kinetics of phase separation for polyethylene glycol–phosphate two‐phase systems

  1995cited by this paper
• Protein extraction by reverse micelles: Studies on the recovery of horseradish peroxidase

  1994cited by this paper
• Protein extraction using reverse micelles: kinetics of protein partitioning

  1994cited by this paper
• Partitioning and purification of α-amylase in aqueous two-phase systems

  1994cited by this paper
• The effects of pH and ionic strength on the partitioning of four proteins in reverse micelle systems

  1994cited by this paper
• Large Scale, In Situ Isolation of Periplasmic IGF–I from E. coli

  1994cited by this paper
• Mathematical modelling and simulation of aqueous two-phase continuous protein extraction.

  1992cited by this paper
• Partitioning and purification of thaumatin in aqueous two-phase systems

  1991cited by this paper
• PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems

  1990cited by this paper
• Partition of Cell Particles and Macromolecules

  1986cited by this paper
• Fractionation of particles and macromolecules in aqueous two-phase systems.

  1961cited by this paper

• Integrated DFT-guided modeling and multi-objective optimization of extractive fermentation for protease production

  2026cites this paper
• Ionic liquids for biomacromolecule purification: from molecular interactions to practical applications.

  2026cites this paper
• Controlling Serum Protein Depletion in Aqueous Biphasic Systems: Interphase Precipitation versus Bottom Phase Retention for Bisphenol A Monitoring

  2026cites this paper
• Phase Separation in Nonaqueous Systems Induced by a Solid Component

  2026cites this paper
• Protein Extraction and Isolation from Legumes and Algae: An Industry Primer

  2025cites this paper
• Aqueous biphasic systems composed of polypropylene glycol and tetraalkylammonium salts: Thermodynamic investigation and application for efficient extraction of biomolecules and dyes

  2025cites this paper
• High-productive recovery and purification of superoxide dismutase from Tetraselmis chuii using integrated ultrasonication–aqueous two-phase system

  2025cites this paper
• Aqueous two-phase separation enables selective purification of mono-PEGylated human serum albumin: influence of process parameters and reagent size

  2025cites this paper
• Aqueous two-phase based simultaneous extraction and purification of cephalopod melanin.

  2025cites this paper
• Study of diclofenac distribution in aqueous two-phase PEG/salt/water systems

  2025cites this paper
• Enhancement of stability and hydrogel properties of type-A gelatin-dextran mixtures via incorporation of whey protein isolate fibrils.

  2025cites this paper
• Phycocyanin as a sustainable future resource: A review on recent advancements, fundamental challenges, opportunities and applications

  2025cites this paper
• Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system

  2025cites this paper
• Polyelectrolyte complex-based materials for separations: progress, challenges and opportunities.

  2025cites this paper
• Health Potential of Glycomacropeptide: A Review Highlighting Isolation, Characterization Methods, Chemistry and Biological Activities

  2025cites this paper
• All‐Aqueous Phase Segregation Integrated Electrochemical Aptamer Biosensor Enables Picomolar Detection of SARS‐CoV‐2 Spike Protein

  2025cites this paper
• Exploring the extraction efficiency of benzyltrialkylammonium chloride-based deep eutectic solvents + inorganic salts based aqueous biphasic systems for flavonoids

  2025cites this paper
• Recovery of Mannosylerythritol Lipids Using Alcohol/Salt Aqueous Two-Phase Extraction

  2025cites this paper
• Aqueous Two‐Phase System (ATPS)‐Based Spore Isolation in Schizosaccharomyces pombe Requires Isp3‐Dependent Surface Hydrophobicity

  2025cites this paper
• Influence of mixing time and agitation speed on partitioning and mass transfer coefficient of cephalexin in aqueous two-phase systems

  2025cites this paper
• Extraction of invertase enzyme from potato tubers by aqueous two-phase system

  2025cites this paper
• Extraction of invertase from Baker’s yeast utilizing PEG8000 with potassium phosphate and potassium citrate in aqueous two-phase systems

  2025cites this paper
• Formation of water-in-water emulsions and microgels in nonionic surfactant + gelatin aqueous mixtures.

  2025cites this paper
• Vegetative and microbial proteins for bioplastics applications – a review in the indian context

  2025cites this paper
• Application of Aqueous Two-Phase System in the Extraction of Invertase Enzyme from Potato Tubers Using PEG8000/Potassium Phosphate

  2025cites this paper
• A Multifunctional Injectable Hydrogel Accelerates Hemostasis and Wound Healing via Synergistic Tissue Adhesion, Antimicrobial Activity, and ROS Scavenging.

  2025cites this paper
• Current experimental, statistical, and mechanistic approaches to optimizing biomolecule separations in aqueous two-phase systems.

  2025cites this paper
• Extraction and purification of short DNA fragments from plasma using aqueous two-phase systems for liquid biopsy

  2025cites this paper
• Assessing biocompatible aqueous two-phase systems for swift, continuous purification of α-amylase through optimization of salt and polymer phases using microfluidic platforms

  2025cites this paper
• Proteomics-on-a-Chip - Microfluidics meets proteomics.

  2025cites this paper
• Influence of the temperature and type of macromolecule on phase diagrams of aqueous two-phase systems

  2024cites this paper
• Production and concentration of keratinases and application of fermentation residual in removing hexavalent chromium

  2024cites this paper
• Membraneless Compartmentalization of Cell-Free Transcription-Translation by Polymer-Assisted Liquid-Liquid Phase Separation.

  2024cites this paper
• Phase Equilibria Assessment and Modelling for Aqueous Two-Phase Systems Composed of Poly(vinylpyrrolidone) or Poly(ethylene glycol)

  2024cites this paper
• Partitioning behavior of short DNA fragments in polymer/salt aqueous two‐phase systems

  2024cites this paper
• Fractionation of Extracellular Polymeric Substances by Aqueous Three-Phase Partitioning Systems

  2024cites this paper
• Advancements in Aqueous Two-Phase Systems for Enzyme Extraction, Purification, and Biotransformation

  2024cites this paper
• Systematic study of chromonic liquid crystal-based complex emulsions and their application in the preparation of silica nanomaterials

  2024cites this paper
• DNA‐Origami‐Armored DNA Condensates

  2024cites this paper
• A Review of Background and Application of ATPSs in Protein and Enzyme Extraction

  2024influential citation
• Partitioning recombinant chitinase from Nicotiana benthamiana by an aqueous two-phase system based on polyethylene glycol and phosphate salts.

  2024cites this paper
• Co-production of 1,3-propanediol and phage phiKpS2 from the glycerol fermentation by Klebsiella pneumoniae

  2024cites this paper
• Extraction of bioactives from pomegranate peels using aqueous biphasic separation (ABPS): An optimization and bioactive profiling

  2024cites this paper
• Dynamic Interfacial Tension Measurement for the Kinetic Study of Eu(III) Extraction in the NaNO3/Tributyl Phosphate System Based on an Increase in Interfacial Tension.

  2024cites this paper
• Utilization and prospect of purification technologies in natural proteins, peptides and recombinant proteins

  2024cites this paper
• Bio-Based, Recyclable, and Form-Stable Thermally Conductive Phase Change Composite Featuring Thermally Induced Flexibility

  2024cites this paper
• Aqueous two-phase systems for the extraction, separation, and recovery of synthetic dyes

  2024cites this paper
• Bioactive Flavonoids Partitioning in a Deep Eutectic Solvent/Surfactant Aqueous Biphasic System: Efficiency and Thermodynamics

  2024cites this paper
• Development of a HPLC system using a phase-separation multiphase flow as an eluent: an influence of column pressure on phase separation and chromatogram at room temperature

  2024cites this paper
• Unravelling the molecular interactions behind the formation of PEG/PPG aqueous two-phase systems.

  2024cites this paper
• Valorization of biomass for food protein via deep eutectic solvent extraction: Understanding the extraction mechanism and impact on protein structure and properties

  2024cites this paper
• Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma

  2024cites this paper
• Sustainable processing of microalgae protein: Design of biphasic partitioning systems based on natural deep eutectic solvents for C-phycocyanin recovery from model aqueous solutions

  2024cites this paper
• Properties of Pickering Stabilized Associative Water‐In‐Water Emulsions Based on Ultra‐High Molecular Weight Polyacrylamides

  2024cites this paper
• Removal of Acetaminophen (Paracetamol) from Water Using Aqueous Two-Phase Systems (ATPSs) Composed of Choline-Amino Acid Ionic Liquids

  2023cites this paper
• Advances in modeling techniques for the production and purification of biomolecules: A comprehensive review.

  2023cites this paper
• Liquid–Liquid Equilibrium Data of Ternary Mixture of Polymer, Sodium Thiosulfate, and Water: Effect of Type and Molar Mass of Macromolecule

  2023cites this paper
• A novel protein purification scheme based on salt inducible self-assembling peptides

  2023cites this paper
• A coarse-grained model for aqueous two-phase systems: Application to ferrofluids.

  2023cites this paper
• Utilization of an aqueous two-phase emulsification to prepare bimodal porous cellulose monolith for efficient adsorption of bovine serum albumin.

  2023cites this paper
• Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b.

  2023cites this paper
• Improved accuracy in pentraxin-3 quantification assisted by aqueous biphasic systems as serum pretreatment strategies.

  2023cites this paper
• Tuning the bis-hydrophilic balance of microgels: A tool to control the stability of water-in-water emulsions.

  2023cites this paper
• Phase-separation multiphase flow: preliminary application to analytical chemistry

  2023cites this paper
• Development of continuous processing platform utilizing aqueous two-phase extraction for purification of monoclonal antibodies.

  2023cites this paper
• A comprehensive perspective on sustainable bioprocessing through extractive fermentation: challenges and prospects

  2023influential citation
• Hydrophilic Polymers: Current Trends and Visions for the Future

  2023cites this paper
• Hydrodynamics of cholinium chloride-based Aqueous Biphasic Systems (ABS): a key study for their industrial implementation

  2023cites this paper
• Enhancing Intracellular Optical Performance and Stability of Engineered Nanomaterials via Aqueous Two-Phase Purification.

  2023cites this paper
• Comparison of extracellular vesicle isolation processes for therapeutic applications

  2023cites this paper
• Extraction of Bovine Serum Albumin by Aqueous Two-Phase System Using PEG4000/Sodium Citrate and PEG8000/Sodium Phosphate

  2023cites this paper
• Extraction of Natural Colorants using Supramolecular Solvents Composed of Triton X-114 and Ionic Liquids

  2023cites this paper
• Protein Hydrolysate from Underutilized Legumes: Unleashing the Potential for Future Functional Foods

  2023cites this paper
• Triblock copolymers as versatile constituents of double stimuli-responsive ionic-liquid-based aqueous biphasic systems

  2023cites this paper
• A survey to evaluate parameters governing the selection and application of extracellular vesicle isolation methods

  2023cites this paper
• Nanosilicate-hydrogel microspheres formed by aqueous two-phase separation for sustained release of small molecules

  2023cites this paper
• Water-in-Water Emulsions Stabilized by Silica Janus Nanosheets.

  2023cites this paper
• Formation of Aqueous Two‐Phase Systems Using Potassium Salt and Deep Eutectic Mixtures for Extraction of Proteins

  2023cites this paper
• Enhancement of Vanillin Partitioning and Recovery in Nanoparticle-based Aqueous Two-phase System Containing PEG and Dextran Polymers

  2023cites this paper
• Production and Recovery of Ectoine: A Review of Current State and Future Prospects

  2023cites this paper
• Synthetic Intracellular Environments: From Basic Science to Applications.

  2023cites this paper
• Phase diagrams of PEG1000,1500,2000,4000,6000 + lithium citrate + water ATPSs, and the partitioning of salbutamol at T = 298.15 K

  2023cites this paper
• Observation of the phase-separation multiphase flow using a polyethylene glycol/phosphate mixed solutions and the aqueous two-phase distribution of red blood cells in the flow system

  2023cites this paper
• Extraction of Gallic Acid and Ferulic Acid for Application in Hair Supplements

  2023cites this paper
• In Situ Incorporation of TiO2@Graphene Oxide (GO) Nanosheets in Polyacrylonitrile (PAN)-Based Membranes Matrix for Ultrafast Protein Separation

  2023cites this paper
• Aqueous Two-Phase Systems Based on Cationic and Anionic Surfactants Mixture for Rapid Extraction and Colorimetric Determination of Synthetic Food Dyes

  2023cites this paper
• Use of Tunable Copolymers in Aqueous Biphasic Systems for Extractive Bioconversion Aimed at Continuous Fructooligosaccharide Production

  2023cites this paper
• Bioactive Compounds from Artichoke and Application Potential

  2023cites this paper
• Extensive studies on physiochemical properties, purification strategies, application of microbial l-amino acid oxidase

  2023cites this paper
• Salting‐out extraction of recombinant κ‐carrageenase and phage T7 released from Escherichia coli cells

  2023cites this paper
• One-step salting-out extraction of bacteriophage from its infection broth of Acinetobacter baumannii.

  2022cites this paper
• Kinetics and mechanism of Eu(III) transfer in tributyl phosphate microdroplet/HNO3 aqueous solution system revealed by fluorescence microspectroscopy

  2022cites this paper
• Production of nascent ribosome precursors within the nucleolar microenvironment of Saccharomyces cerevisiae.

  2022cites this paper
• Separation and Purification of Astragalus membranaceus Polysaccharides by Deep Eutectic Solvents-Based Aqueous Two-Phase System

  2022cites this paper
• An economical and specific purification process of human haptoglobin 1-1

  2022cites this paper
• Polymeric Aqueous Two Phase System with an Immobilized Phase by Cross-Linking

  2022cites this paper
• Sustainable Upcycling of Fisheries and Aquaculture Wastes Using Fish-Derived Cold-Adapted Proteases

  2022cites this paper
• An injectable hemostatic PEG-based hydrogel with on-demand dissolution features for emergency care.

  2022cites this paper
• Ultrasound-assisted processing: Science, technology and challenges for the plant-based protein industry

  2022cites this paper
• Recovery Techniques Enabling Circular Chemistry from Wastewater

  2022cites this paper