Chromatin assembly: Journey to the CENter of the chromosome

All eukaryotic genomes are packaged into basic units of DNA wrapped around histone proteins called nucleosomes. The ability of histones to specify a variety of epigenetic states at defined chromatin domains is essential for cell survival. The most distinctive type of chromatin is found at centromeres, which are marked by the centromere-specific histone H3 variant CENP-A. Many of the factors that regulate CENP-A chromatin have been identified; however, our understanding of the mechanisms of centromeric nucleosome assembly, maintenance, and reorganization remains limited. This review discusses recent insights into these processes and draws parallels between centromeric and noncentromeric chromatin assembly mechanisms.

• Publication year

  2016

• Venue

  Journal of Cell Biology

• Publication date

  2016-07-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201605005PMID 27377247PMCID 4932374
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2016influential reference
• Centromere localization and function of Mis18 requires Yippee‐like domain‐mediated oligomerization

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• Centromeric Transcription Regulates Aurora-B Localization and Activation.

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  2016cited by this paper
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  2016cited by this paper
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  2016cited by this paper
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  2015cited by this paper
• Plos One

  2015cited by this paper
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  2015influential reference
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  2015influential reference
• Stable complex formation of CENP-B with the CENP-A nucleosome

  2015cited by this paper
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  2015influential reference
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  2015cited by this paper
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  2014cited by this paper
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  2014cited by this paper
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  2014influential reference
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  2014influential reference
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  2014cited by this paper
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  2013influential reference
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  2012influential reference
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  2012influential reference
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  2012influential reference
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  2011cited by this paper
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  2011cited by this paper
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• Identification of Noncoding Transcripts from within CENP-A Chromatin at Fission Yeast Centromeres*

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  2011influential reference
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  2011cited by this paper
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  2011influential reference
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  2009influential reference
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  2009influential reference
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  2009influential reference
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  2008influential reference
• Dynamics of inner kinetochore assembly and maintenance in living cells

  2008cited by this paper
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  2007influential reference
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  2007influential reference
• Tetrameric Structure of Centromeric Nucleosomes in Interphase Drosophila Cells

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  2007cited by this paper

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• The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary

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  2020cites this paper
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  2019cites this paper
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