The fluorometric measurement of the nucleotides of riboflavin and their concentration in tissues.

SUMMARY 1. A convenient fluorometric procedure is described which permits separate measurement of riboflavin, riboflavin monophosphate, and ribo- flavin dinucleotide. The measurement of the dinucleotide is based on the fact that it has much less fluorescence than riboflavin, and that it can be readily converted into riboflavin monophosphate which exhibits the same fluorescence as riboflavin. Riboflavin is distinguished from its two deriva- tives by its very much greater partition coefficient between beneyl alcohol and water. 2. Data are presented concerning some of the properties of riboflavin dinucleotide, particularly its rate of hydrolysis in acid and the influence of pH and solvent on its fluorescence. 3. Evidence is given that riboflavin monophosphate and dinucleotide account for practically all of the riboflavin of rat kidney. 4. The riboflavin dinucleotide content of liver, kidney, and heart was measured both fluorometrically and enzymatically with concurring re- sults. 5. The data are given for the concentration of the three riboflavin frac- tions in five major rat tissues, both normal and riboflavin-deficient, and for the total riboflavin and dinucleotide concentrations in sixteen others. Free riboflavin was found to be present in quantitatively insignificant amounts. The dinucleotide accounted in general for 70 to 90 per cent of the total riboflavin.

• Publication year

  1949

• Venue

  Journal of Biological Chemistry

• Publication date

  1949-09-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)56696-5PMID 18135810
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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