Confinement-induced liquid crystalline transitions in amyloid fibril cholesteric tactoids

Chirality is ubiquitous in nature and plays crucial roles in biology, medicine, physics and materials science. Understanding and controlling chirality is therefore an important research challenge with broad implications. Unlike other chiral colloids, such as nanocellulose or filamentous viruses, amyloid fibrils form nematic phases but appear to miss their twisted form, the cholesteric or chiral nematic phases, despite a well-defined chirality at the single fibril level. Here we report the discovery of cholesteric phases in amyloids, using β-lactoglobulin fibrils shortened by shear stresses. The physical behaviour of these new cholesteric materials exhibits unprecedented structural complexity, with confinement-driven ordering transitions between at least three types of nematic and cholesteric tactoids. We use energy functional theory to rationalize these results and observe a chirality inversion from the left-handed amyloids to right-handed cholesteric droplets. These findings deepen our understanding of cholesteric phases, advancing their use in soft nanotechnology, nanomaterial templating and self-assembly.Left-handed amyloid fibrils form nematic and right-handed cholesteric tactoids with confinement-induced transitions from an ordered to an ordered state.

• Publication year

  2017

• Venue

  Nature Nanotechnology

• Publication date

  2017-04-17

• Fields of study

  Chemistry, Medicine, Materials Science, Physics

• Identifiers
  DOI 10.1038/s41565-018-0071-9arXiv 1704.04936PMID 29556006
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Twisting with a twist: supramolecular helix fluctuations in chiral nematics.

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• Amyloid Fibrils as Building Blocks for Natural and Artificial Functional Materials

  2016cited by this paper
• Experimental realization of crossover in shape and director field of nematic tactoids.

  2015cited by this paper
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  2015cited by this paper
• Spontaneous emergence of chirality in achiral lyotropic chromonic liquid crystals confined to cylinders

  2015cited by this paper
• Density functional theory for chiral nematic liquid crystals.

  2014cited by this paper
• Left or right cholesterics? A matter of helix handedness and curliness.

  2014cited by this paper
• Polymorphism complexity and handedness inversion in serum albumin amyloid fibrils.

  2013cited by this paper
• Chirality in Liquid Crystals

  2013cited by this paper
• Particles trapped at the droplet interface in water-in-water emulsions.

  2012cited by this paper
• Reconfigurable self-assembly through chiral control of interfacial tension

  2012cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2011cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2010cited by this paper
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  2009cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2006cited by this paper
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  2005cited by this paper
• Protein folding and misfolding

  2003cited by this paper
• Shape and director-field transformation of tactoids.

  2003cited by this paper
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  2002cited by this paper
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  2000cited by this paper
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  1999cited by this paper
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  1998cited by this paper
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  1992cited by this paper
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  1992cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1988cited by this paper
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  1984cited by this paper
• Liquid-crystalline ordering in the solution of long persistent chains

  1981cited by this paper
• Amyloid deposits and amyloidosis. The beta-fibrilloses (first of two parts).

  1980cited by this paper
• Theory of piezoelectricity in nematic liquid crystals, and of the cholesteric ordering

  1976cited by this paper
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  1961cited by this paper

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  2021cites this paper
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  2020cites this paper