lac repressor forms loops with linear DNA carrying two suitably spaced lac operators.

No abstract is available for this paper.

• Publication year

  1987

• Venue

  EMBO Journal

• Publication date

  1987-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1002/j.1460-2075.1987.tb02390.xPMID 3301328PMCID PMC553955
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Upstream operators enhance repression of the lac promoter.

  1986cited by this paper
• Requirement of stereospecific alignments for initiation from the simian virus 40 early promoter

  1986cited by this paper
• DNA bending at adenine · thymine tracts

  1986cited by this paper
• Gene regulation by proteins acting nearby and at a distance

  1986cited by this paper
• DNA loops induced by cooperative binding of lambda repressor.

  1986cited by this paper
• The DNA loop model for ara repression: AraC protein occupies the proposed loop sites in vivo and repression-negative mutations lie in these same sites.

  1986cited by this paper
• Thermodynamic analysis of the lactose repressor-operator DNA interaction.

  1986cited by this paper
• Curved DNA: design, synthesis, and circularization.

  1986cited by this paper
• Regulation of the operon encoding ribonucleotide reductase in Escherichia coli: evidence for both positive and negative control.

  1986cited by this paper
• DNA‐protein recognition: demonstration of three genetically separated operator elements that are required for repression of the Escherichia coli deoCABD promoters by the DeoR repressor.

  1986cited by this paper
• Cooperative binding of lambda repressors to sites separated by integral turns of the DNA helix.

  1986cited by this paper
• Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter.

  1986cited by this paper
• The upstream operator of the Escherichia coli galactose operon is sufficient for repression of transcription initiated at the cyclic AMP‐stimulated promoter.

  1986cited by this paper
• Two operator sites separated by 599 base pairs are required for deoR repression of the deo operon of Escherichia coli.

  1985cited by this paper
• DNA bending and its relation to nucleosome positioning.

  1985cited by this paper
• Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

  1985cited by this paper
• Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA

  1985cited by this paper
• Thermodynamic origins of specificity in the lac repressor-operator interaction. Adaptability in the recognition of mutant operator sites.

  1985cited by this paper
• An operator at -280 base pairs that is required for repression of araBAD operon promoter: addition of DNA helical turns between the operator and promoter cyclically hinders repression.

  1984cited by this paper
• Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling.

  1984cited by this paper
• Kinetics and mechanism in the reaction of gene regulatory proteins with DNA.

  1984cited by this paper
• One-step purification of hybrid proteins which have beta-galactosidase activity.

  1984cited by this paper
• Role of DNA regions flanking the tryptophan promoter of Escherichia coli. II. Insertion of lac operator fragments.

  1984cited by this paper
• Possible ideal lac operator: Escherichia coli lac operator-like sequences from eukaryotic genomes lack the central G X C pair.

  1984cited by this paper
• Demonstration of two operator elements in gal: in vitro repressor binding studies.

  1984cited by this paper
• On the different binding affinities of CRP at the lac, gal and malT promoter regions.

  1983cited by this paper
• A perfectly symmetric lac operator binds the lac repressor very tightly.

  1983cited by this paper
• A control element within a structural gene: the gal operon of Escherichia coli.

  1983cited by this paper
• Energetics of DNA twisting. I. Relation between twist and cyclization probability.

  1983cited by this paper
• Characterization of two mutations in the Escherichia coli galE gene inactivating the second galactose operator and comparative studies of repressor binding.

  1983cited by this paper
• Energetics of DNA twisting. II. Topoisomer analysis.

  1983cited by this paper
• Escherichia coli lac repressor is elongated with its operator DNA binding domains located at both ends.

  1982cited by this paper
• Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

  1982cited by this paper
• Lac repressor - lac operator interaction. Circular dichroism study.

  1981cited by this paper
• A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system

  1981cited by this paper
• Diffusion-driven mechanisms of protein translocation on nucleic acids. 2. The Escherichia coli repressor--operator interaction: equilibrium measurements.

  1981cited by this paper
• DNA flexibility studied by covalent closure of short fragments into circles.

  1981cited by this paper
• Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.

  1981cited by this paper
• Sequence of the lactose permease gene

  1980cited by this paper
• DNA binding characteristics of lactose repressor and the trypsin-resistant core repressor.

  1980cited by this paper
• Construction, isolation and implications of repressor-galactosidase - beta-galactosidase hybrid molecules.

  1977cited by this paper
• A new method for sequencing DNA.

  1977cited by this paper
• Studies on the lactose operon. III. Visualization and physical mapping of the lactose repressor-operator complex.

  1976cited by this paper
• The functional repressor parts of a tetrameric lac repressor-beta-galactosidase chimaera are organized as dimers.

  1976cited by this paper
• Lac repressor and lac operator.

  1975cited by this paper
• The location of the repressor binding sites in the lac operon.

  1974cited by this paper
• A new preparation method for dark-field electron microscopy of biomacromolecules.

  1971cited by this paper
• The lac repressor-operator interaction. 3. Kinetic studies.

  1970cited by this paper

• Repression via DNA looping by the Gram-positive global transcriptional regulator ScoC from Geobacillus

  2025cites this paper
• Reversible DNA condensation drives natural transformation

  2025cites this paper
• Unified Memory Kernel for Polymer Dynamics: Coupling Hydrodynamic and Excluded Volume Effects in Generalized Langevin Equations

  2025cites this paper
• Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coli

  2024cites this paper
• Transcription Attenuation in Synthetic Promoters in Nonoverlapping Tandem Formation

  2024cites this paper
• Image-based 3D genomics through chromatin tracing

  2024cites this paper
• Engineering allosteric transcription factors guided by the LacI topology.

  2023cites this paper
• Loops are geometric catalysts for DNA integration

  2023cites this paper
• Transcription attenuation in synthetic promoters in tandem formation

  2023cites this paper
• Silencing Transcription from an Influenza Reverse Genetics Plasmid in E. coli Enhances Gene Stability

  2023cites this paper
• Explicit analytical form for memory kernel in the generalized Langevin equation for end-to-end vector of Rouse chains.

  2022cites this paper
• The loopometer: a quantitative in vivo assay for DNA-looping proteins

  2021cites this paper
• The rDNA is biomolecular condensate formed by polymer–polymer phase separation and is sequestered in the nucleolus by transcription and R-loops

  2021cites this paper
• Segregational Drift Constrains the Evolutionary Rate of Prokaryotic Plasmids

  2021cites this paper
• Designed architectural proteins that tune DNA looping in bacteria

  2021cites this paper
• Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

  2020cites this paper
• Mechanical Flexibility of DNA: A Quintessential Tool for DNA Nanotechnology

  2020cites this paper
• Evolution of Regulated Transcription

  2020cites this paper
• Long‐term evolution of the natural isolate of Escherichia coli 536 in the mouse gut colonized after maternal transmission reveals convergence in the constitutive expression of the lactose operon

  2019cites this paper
• Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production.

  2019cites this paper
• Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over‐looked physiological factors

  2019cites this paper
• Cyclization and Relaxation Dynamics of Finite-Length Collapsed Self-Avoiding Polymers.

  2019cites this paper
• Bridging chromatin structure and function over a range of experimental spatial and temporal scales by molecular modeling

  2019cites this paper
• Dependence of DNA looping on Escherichia coli culture density.

  2019cites this paper
• Measurement of Mesoscale Conformational Dynamics of Freely Diffusing Molecules with Tracking FCS.

  2018cites this paper
• Biomolecular Assemblies: Moving from Observation to Predictive Design.

  2018cites this paper
• Structure-guided approach to site-specific fluorophore labeling of the lac repressor LacI

  2018cites this paper
• Reconciling in vitro and in vivo activities of engineered, LacI-based repressor proteins: Contributions of DNA looping and operator sequence variation

  2018cites this paper
• Computational modeling of DNA elastic energy to predict structure and topology of protein mediated DNA loops

  2017cites this paper
• Intramolecular dynamics of single molecules in free diffusion

  2017cites this paper
• The mechanics of DNA loops bridged by proteins unveiled by single-molecule experiments.

  2017cites this paper
• Glutamine synthetase stabilizes the binding of GlnR to nitrogen fixation gene operators

  2017cites this paper
• Self-consistent theory of transcriptional control in complex regulatory architectures

  2017cites this paper
• Polymer physics of nuclear organization and function

  2016cites this paper
• Pseudomonas aeruginosa AmrZ Binds to Four Sites in the algD Promoter, Inducing DNA-AmrZ Complex Formation and Transcriptional Activation

  2016cites this paper
• Insights into genome architecture deduced from the properties of short Lac repressor-mediated DNA loops

  2016influential citation
• Direct observation of a 91 bp LacI‐mediated, negatively supercoiled DNA loop by atomic force microscope

  2016cites this paper
• Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects

  2016cites this paper
• Protein-induced DNA linking number change by sequence-specific DNA binding proteins and its biological effects

  2016cites this paper
• Insights into genome architecture deduced from the properties of short Lac repressor-mediated DNA loops

  2016cites this paper
• Supercoiling Effects on Short-Range DNA Looping in E. coli

  2016cites this paper
• Coupled binding-bending-folding: The complex conformational dynamics of protein-DNA binding studied by atomistic molecular dynamics simulations.

  2015cites this paper
• Exploring LacI-DNA dynamics by multiscale simulations using the SIRAH force field.

  2015cites this paper
• High-resolution mapping of architectural DNA binding protein facilitation of a DNA repression loop in Escherichia coli

  2015cites this paper
• Engineering of genetic control tools in Synechocystis sp. PCC 6803 using rational design techniques.

  2015cites this paper
• DNA looping-dependent autorepression of LEE1 P1 promoters by Ler in enteropathogenic Escherichia coli (EPEC)

  2014cites this paper
• Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

  2014cites this paper
• Tethered particle motion reveals that LacI·DNA loops coexist with a competitor-resistant but apparently unlooped conformation.

  2014cites this paper
• Allostery and applications of the lac repressor

  2014cites this paper
• A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping.

  2014cites this paper
• From adjacent activation in Escherichia coli and DNA cyclization to eukaryotic enhancers: the elements of a puzzle

  2014cites this paper
• What Controls DNA Looping?

  2014cites this paper
• Bacterial promoter repression by DNA looping without protein–protein binding competition

  2014cites this paper
• Arabidopsis SEPALLATA proteins differ in cooperative DNA-binding during the formation of floral quartet-like complexes

  2014cites this paper
• EVALUATING IN VITRO AND IN VIVO LOOPING EFFICIENCIES OF ARTIFICIAL DNA-BINDING PROTEINS

  2014cites this paper
• Rationally designed coiled-coil DNA looping peptides control DNA topology

  2013cites this paper
• Simulating the Mechanics of Protein-Induced DNA Looping and Protein-Constrained DNA Buckling.

  2013cites this paper
• DNA Looping in Prokaryotes: Experimental and Theoretical Approaches

  2013cites this paper
• Weak operator binding enhances simulated lac repressor‐mediated DNA looping

  2013cites this paper
• Sequence dependence of transcription factor-mediated DNA looping

  2012cites this paper
• Mechanobiology of Short DNA Molecules: A Single Molecule Perspective

  2012cites this paper
• Mechanism of promoter repression by Lac repressor–DNA loops

  2012cites this paper
• A regulatory element for interneuron progenitors in the developing vertebrate central nervous system

  2012cites this paper
• Intra-nuclear mobility and target search mechanisms of transcription factors: a single-molecule perspective on gene expression.

  2012cites this paper
• Novel insights from hybrid LacI/GalR proteins: family-wide functional attributes and biologically significant variation in transcription repression

  2012cites this paper
• FRET studies of a landscape of Lac repressor-mediated DNA loops

  2012cites this paper
• Stratégie du leurre moléculaire en thérapie génique : ciblage de facteurs de transcription par un minicercle d'ADN multisites et étude de l'interaction entre l'ADN platiné et NF-κB

  2012cites this paper
• Differentiating a Landscape of Lac Repressor Mediated DNA Loops Using FRET

  2011cites this paper
• Structural insights into transcription complexes.

  2011cites this paper
• On the continuum formulation for modeling DNA loop formation

  2011cites this paper
• Biochemical Studies of the CTCF Insulator Protein: Determination of Protein Interactions with CTCF using Tandem Affinity Purification, Characterization of its Post-translational Modification by the Small Ubiquitin-like Modifier Proteins and Studies of CTCF DNA Looping Ability

  2011cites this paper
• EspR, a key regulator of Mycobacterium tuberculosis virulence, adopts a unique dimeric structure among helix-turn-helix proteins

  2011cites this paper
• DNA modeling reveals an extended lac repressor conformation in classic in vitro binding assays.

  2011influential citation
• Quantitative methods for measuring DNA flexibility in vitro and in vivo.

  2011cites this paper
• DNA bending and looping in the transcriptional control of bacteriophage phi29.

  2010cites this paper
• The evolutionary origin of "floral quartets" : clues from molecular interactions of orthologues of floral homeotic proteins from the gymnosperm Gnetum gnemon

  2010cites this paper
• The Topology and Organization of Eukaryotic Chromatin

  2010cites this paper
• Single-molecule Approaches to Probe the Structure, Kinetics, and Thermodynamics of Nucleoprotein Complexes That Regulate Transcription*

  2010cites this paper
• Regulating gene expression through DNA mechanics: Tightly looped DNA represses transcription

  2010cites this paper
• Coordinate control of gene expression noise and interchromosomal interactions in a MAPK pathway

  2010cites this paper
• Role of HU in Regulation of gal Promoters

  2010cites this paper
• Mechanics and function of DNA looping and supercoiling

  2010influential citation
• The CTCF insulator protein forms an unusual DNA structure

  2010cites this paper
• DNA curvature and flexibility in vitro and in vivo

  2010influential citation
• Studies of Sequence-Nonspecific HMGB DNA-Binding Proteins

  2010cites this paper
• DNA linking number change induced by sequence-specific DNA-binding proteins

  2010cites this paper
• Bending the rules of transcriptional repression: tightly looped DNA directly represses T7 RNA polymerase.

  2010cites this paper
• Tetramer opening in LacI-mediated DNA looping

  2009cites this paper
• Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes.

  2009cites this paper
• Protein–protein interactions between CbbR and RegA (PrrA), transcriptional regulators of the cbb operons of Rhodobacter sphaeroides

  2009cites this paper
• Mechanisms of distant enhancer action on DNA and in chromatin

  2009cites this paper
• Enhancement of DNA flexibility in vitro and in vivo by HMGB box A proteins carrying box B residues.

  2009cites this paper
• Molecular geometry of CsrA (RsmA) binding to RNA and its implications for regulated expression.

  2009cites this paper
• Reconstitution of ‘floral quartets’ in vitro involving class B and class E floral homeotic proteins

  2009cites this paper
• Computational analysis of looping of a large family of highly bent DNA by LacI.

  2008cites this paper
• Minicircle‐DNA production by site specific recombination and protein–DNA interaction chromatography

  2008cites this paper
• Ligand-induced conformational changes and conformational dynamics in the solution structure of the lactose repressor protein.

  2008cites this paper
• Concentration and Length Dependence of DNA Looping in Transcriptional Regulation

  2008cites this paper
• The class E floral homeotic protein SEPALLATA3 is sufficient to loop DNA in ‘floral quartet’-like complexes in vitro

  2008influential citation
• Description of nonspecific DNA-protein interaction and facilitated diffusion with a dynamical model.

  2008cites this paper