The ZASP-like Motif in Actinin-associated LIM Protein Is Required for Interaction with the α-Actinin Rod and for Targeting to the Muscle Z-line*

The Z-line is a specialized structure connecting adjacent sarcomeres in muscle cells. α-Actinin cross-links actin filaments in the Z-line. Several PDZ-LIM domain proteins localize to the Z-line and interact with α-actinin. Actinin-associated LIM protein (ALP), C-terminal LIM domain protein (CLP36), and Z band alternatively spliced PDZ-containing protein (ZASP) have a conserved region named the ZASP-like motif (ZM) between PDZ and LIM domains. To study the interactions and function of ALP we used purified recombinant proteins in surface plasmon resonance measurements. We show that ALP and α-actinin 2 have two interaction sites. The ZM motif was required for the interaction of ALP internal region with the α-actinin rod and for targeting of ALP to the Z-line. The PDZ domain of ALP bound to the C terminus of α-actinin. This is the first indication that the ZM motif would have a direct role in a protein-protein interaction. These results suggest that the two interaction sites of ALP would stabilize certain conformations of α-actinin 2 that would strengthen the Z-line integrity.

• Publication year

  2004

• Venue

  Journal of Biological Chemistry

• Publication date

  2004-06-18

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.M401871200PMID 15084604
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The two interaction sites of ALP are suggested to stabilize α-Actinin 2 conformations and strengthen Z-line integrity.

  Confidence 0.78

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ZASP-like motif (ZM) is required for targeting Actinin-associated LIM protein (ALP) to the Z-line and is implicated in direct protein-protein interaction.

  Confidence 0.90

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• The PDZ domain of ALP binds the α-Actinin C terminus.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ZASP-like motif (ZM) is required for binding between the internal region of Actinin-associated LIM protein (ALP) and the α-Actinin rod.

  Confidence 0.96

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• Actinin-associated LIM protein (ALP) and α-Actinin 2 interact through two distinct binding sites.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

• actinin-associated lim protein (alp)

  protein

  A PDZ-LIM domain protein examined for localization to the muscle Z-line and binding to α-actinin.

  Aliases: ALP

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• pdz domain of alp

  protein domain

  The N-terminal PDZ domain of ALP that recognizes partner C-terminal sequences.

  Aliases: ALP PDZ domain

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• zasp-like motif (zm)

  motif

  A conserved sequence segment located between the PDZ and LIM domains of PDZ-LIM proteins.

  Aliases: ZM motif

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• z-line

  cellular structure

  The specialized boundary structure that connects adjacent sarcomeres in muscle cells.

  Aliases: Z band, muscle Z-line

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• α-actinin 2

  protein

  A sarcomeric α-actinin isoform that cross-links actin filaments and serves as the binding partner in the interaction assays.

  Aliases: alpha-actinin 2, α-actinin2

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• α-actinin c terminus

  protein region

  The terminal end of α-actinin that binds the ALP PDZ domain.

  Aliases: C terminus of α-actinin

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• α-actinin rod

  protein region

  The rod region of α-actinin that interacts with the internal region of ALP.

  Aliases: rod region of α-actinin

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

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