The Structure of Sucrose Synthase-1 from Arabidopsis thaliana and Its Functional Implications*

Background: Sucrose flux in plants is partly achieved through sucrose synthesis and cleavage catalyzed by sucrose synthase. Results: The crystal structure of sucrose synthase from Arabidopsis thaliana has been determined. Conclusion: The x-ray structures provide insights into the enzymology and regulation of sucrose synthase. Significance: The structures suggest how sucrose synthase interacts with cellular targets, such as membranes, organelles, and cytoskeletal actin. Sucrose transport is the central system for the allocation of carbon resources in vascular plants. During growth and development, plants control carbon distribution by coordinating sites of sucrose synthesis and cleavage in different plant organs and different cellular locations. Sucrose synthase, which reversibly catalyzes sucrose synthesis and cleavage, provides a direct and reversible means to regulate sucrose flux. Depending on the metabolic environment, sucrose synthase alters its cellular location to participate in cellulose, callose, and starch biosynthesis through its interactions with membranes, organelles, and cytoskeletal actin. The x-ray crystal structure of sucrose synthase isoform 1 from Arabidopsis thaliana (AtSus1) has been determined as a complex with UDP-glucose and as a complex with UDP and fructose, at 2.8- and 2.85-Å resolutions, respectively. The AtSus1 structure provides insights into sucrose catalysis and cleavage, as well as the regulation of sucrose synthase and its interactions with cellular targets.

• Publication year

  2011

• Venue

  Journal of Biological Chemistry

• Publication date

  2011-08-24

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1074/jbc.M111.275974PMID 21865170PMCID PMC3195635
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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