SCIENCE@home

ABSTRACT

: Streptococcus mutans ( S. mutans ) antisense vicK RNA (AS vicK ) is a non-coding RNA that regulates cariogenic virulence and metabolic activity. Dimethylaminohexadecyl methacrylate (DMAHDM), a quaternary ammonium methacrylate used in dental materials, has strong antibacterial activity. This study examined the effects of S. mutans AS vicK on DMAHDM susceptibility and their combined impact on inhibiting S. mutans biofilm formation and protecting enamel hardness. The parent S. mutans UA159 and AS vicK overexpressing S. mutans (AS vicK ) were tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations for planktonic bacteria (MBC-P) and biofilms (MBC-B) were measured. As the AS vicK MBC-B was 175 µ g/mL, live/dead staining, metabolic activity (MTT), colony-forming units (CFUs), biofilm biomass, polysaccharide, and lactic acid production were investigated at 175 µ g/mL and 87.5 µ g/mL. The MIC, MBC-P, and MBC-B values for DMAHDM for the AS vicK strain were half those of the UA159 strain. In addition, combining S. mutans AS vicK with DMAHDM resulted in a significant 4-log CFU reduction ( p < 0.05), with notable decreases in polysaccharide levels and lactic acid production. In the in vitro cariogenic model, the combination achieved the highest enamel hardness at 67.1% of sound enamel, while UA159 without DMAHDM had the lowest at 16.4% ( p < 0.05). Thus, S. mutans AS vicK enhanced DMAHDM susceptibility, and their combination effectively inhibited biofilm formation and minimized enamel demineralization. The S. mutans AS vicK + DMAHDM combination shows great potential for anti-caries dental applications.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-100 of 495 references · Page 1 of 5

12345
Next

CITED BY

Showing 1-100 of 407 citing papers · Page 1 of 5

12345
Next