Early and late effects of prenatal corticosteroid treatment on the microRNA profiles of lung tissue in rats

Glucocorticoids have been administered to mothers at risk of premature delivery to induce maturation of preterm fetal lungs and prevent the development of respiratory distress syndrome. Micro (mi)RNAs serve various crucial functions in cell proliferation, differentiation and organ development; however, few studies have demonstrated an association between miRNAs and lung development. The aim of the present study was to investigate alterations in the miRNA profiles of rat lung tissue following prenatal glucocorticoid therapy for fetal lung development. The differences in miRNA expression profiles were compared between postnatal days 7 (D7) and 120 (D120) rat lung tissues, followed by validation using reverse transcription-quantitative polymerase chain reaction. The miRNA profiles of rat lung tissues following prenatal dexamethasone (DEX) therapy were also investigated. miRNAs with 2-fold changes were selected for further analysis. At D120, 6 upregulated and 6 downregulated miRNAs were detected, compared with D7. Among these differentially expressed miRNAs, miR-101-3p and miR-99b-5p were associated with the lowest and highest expressions of miRNA at D7, respectively. A limited impact on the miRNA profiles of rat lung tissues was observed following prenatal DEX treatment, which may help to further clarify the mechanisms underlying normal lung development. However, the results of the present study cannot entirely elucidate the effects of prenatal DEX treatment on the lung development of premature infants, and further studies investigating the impact of prenatal corticosteroids on fetal lung miRNA profiles are required.

• Publication year

  2016

• Venue

  Experimental and Therapeutic Medicine

• Publication date

  2016-01-13

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3892/etm.2016.2992PMID 26997989PMCID 4774352
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Expression analysis of serum microRNAs in idiopathic pulmonary fibrosis.

  2014cited by this paper
• Downregulation of miR-101-3p by hepatitis B virus promotes proliferation and migration of hepatocellular carcinoma cells by targeting Rab5a

  2014cited by this paper
• Functional analysis of miR-101-3p and Rap1b involved in hepatitis B virus-related hepatocellular carcinoma pathogenesis.

  2014cited by this paper
• Prenatal dexamethasone exposure in rats results in long‐term epigenetic histone modifications and tumour necrosis factor‐α production decrease

  2014cited by this paper
• Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA‐26a

  2014cited by this paper
• miRSeq: A User-Friendly Standalone Toolkit for Sequencing Quality Evaluation and miRNA Profiling

  2014cited by this paper
• Role of microRNAs in lung development and pulmonary diseases

  2013cited by this paper
• Cross-talk between MET and EGFR in non-small cell lung cancer involves miR-27a and Sprouty2

  2013cited by this paper
• Distinct microRNA Expression Profile in Prostate Cancer Patients with Early Clinical Failure and the Impact of let-7 as Prognostic Marker in High-Risk Prostate Cancer

  2013cited by this paper
• Lamin A/C Haploinsufficiency Modulates the Differentiation Potential of Mouse Embryonic Stem Cells

  2013cited by this paper
• Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells

  2012cited by this paper
• Regulation of lung surfactant secretion by microRNA-150.

  2012cited by this paper
• MiR-101 and miR-144 Regulate the Expression of the CFTR Chloride Channel in the Lung

  2012cited by this paper
• Upregulation of miR-27a contributes to the malignant transformation of human bronchial epithelial cells induced by SV40 small T antigen

  2011cited by this paper
• MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2

  2011cited by this paper
• microRNA-101 inhibits lung cancer invasion through the regulation of enhancer of zeste homolog 2.

  2011cited by this paper
• miR-130 Suppresses Adipogenesis by Inhibiting Peroxisome Proliferator-Activated Receptor γ Expression

  2010cited by this paper
• Microrna let-7: an emerging next-generation cancer therapeutic

  2010cited by this paper
• Potential uses of microRNA in lung cancer diagnosis, prognosis, and therapy.

  2009cited by this paper
• Low-level expression of microRNAs let-7d and miR-205 are prognostic markers of head and neck squamous cell carcinoma.

  2009cited by this paper
• MicroRNA-127 modulates fetal lung development.

  2009cited by this paper
• Micro‐RNA‐375 inhibits lung surfactant secretion by altering cytoskeleton reorganization

  2009influential reference
• Antenatal Corticosteroids for Accelerating Fetal Lung Maturation for Women at Risk of Preterm Birth

  2007cited by this paper
• Maternally imprinted microRNAs are differentially expressed during mouse and human lung development

  2007cited by this paper
• Hippo signaling in organ size control.

  2007cited by this paper
• Mechanisms underlying the role of glucocorticoids in the early life programming of adult disease.

  2007cited by this paper
• RAS is regulated by the let-7 microRNA family.

  2005cited by this paper
• Intergenerational consequences of fetal programming by in utero exposure to glucocorticoids in rats.

  2005cited by this paper
• Reduced Expression of the let-7 MicroRNAs in Human Lung Cancers in Association with Shortened Postoperative Survival

  2004cited by this paper
• Different antigens trigger different Th1/Th2 reactions in neonatal mononuclear cells (MNCs) relating to T‐bet/GATA‐3 expression

  2003cited by this paper
• Programmed cell death contributes to postnatal lung development.

  1998cited by this paper
• Impact of advances in physiology, biochemistry, and molecular biology on pulmonary disease in neonates.

  1998cited by this paper
• Prenatal glucocorticoid and T4 effects on lung morphology in preterm lambs.

  1997cited by this paper
• Effects of antenatal and postnatal corticosteroids on the preterm lung

  1997cited by this paper
• Cloning and Expression of Glucocorticoid-induced Genes in Fetal Rat Lung Fibroblasts

  1995cited by this paper
• Cloning and expression of glucocorticoid-induced genes in fetal rat lung fibroblasts. Transforming growth factor-beta 3.

  1995cited by this paper
• The role of cortisol in preparing the fetus for birth.

  1994cited by this paper
• Corticosteroids, Thyrotropin-Releasing Hormone, and Antioxidant Enzymes in Preterm Lamb Lungs

  1991cited by this paper
• Regulation of fetal lung maturation.

  1990cited by this paper
• The effects of corticosteroid administration before preterm delivery: an overview of the evidence from controlled trials

  1990cited by this paper
• Betamethasone and the Rhesus Fetus: Effect on Lung Morphometry and Connective Tissue

  1981cited by this paper

• Epigenetic effects of endogenous and exogenous glucocorticosteroids during pregnancy on the offspring: a systematic-narrative review

  2025cites this paper
• Lung regeneration with rat fetal lung implantation and promotion of alveolar stem cell differentiation by corticosteroids

  2023cites this paper
• Association of umbilical cord blood miR-375 with neonatal respiratory distress syndrome and adverse neonatal outcomes in premature infants.

  2022cites this paper
• A History of Preterm Delivery Is Associated with Aberrant Postpartal MicroRNA Expression Profiles in Mothers with an Absence of Other Pregnancy-Related Complications

  2021cites this paper
• Resveratrol intake during pregnancy and lactation re-programs adiposity and ameliorates leptin resistance in male progeny induced by maternal high-fat/high sucrose plus postnatal high-fat/high sucrose diets via fat metabolism regulation

  2020cites this paper
• Obesity programmed by prenatal dexamethasone and postnatal high-fat diet leads to distinct alterations in nutrition sensory signals and circadian-clock genes in visceral adipose tissue

  2019cites this paper
• Prenatal dexamethasone and postnatal high-fat diet have a synergistic effect of elevating blood pressure through a distinct programming mechanism of systemic and adipose renin–angiotensin systems

  2018cites this paper
• Recent advances in our understanding of the mechanisms of late lung development and bronchopulmonary dysplasia.

  2017cites this paper