Biosynthetic and structural properties of endothelial cell type VIII collagen.

A highly unusual endothelial cell collagen (Sage, H., Pritzl, P., and Bornstein, P., (1980) Biochemistry 19, 5747-5755) has been characterized in greater detail. Pulse-chase experiments with bovine aortic endothelial cells revealed two nondisulfide-bonded collagens, of apparent chain Mr = 177,000 and 125,000, with an estimated synthesis and secretion time of 75 min. Stepwise, quantitative processing to stable lower molecular weight forms as described for type I procollagen was not observed. Endothelial collagen was secreted over a temperature range of 24-37 degrees C and, prior to heat denaturation, did not display affinity for a gelatin-binding fragment of fibronectin coupled to Sepharose. The presence of a pepsin-resistant domain (Mr = 50,000) in both the soluble and cell layer-associated forms of this protein was shown by ion exchange chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Endothelial collagen was cleaved by vertebrate collagenase into several discrete fragments that differed in molecular weight from the characteristic alpha A and alpha B fragments generated from the interstitial collagens. Nontriple helical domains corresponding to the NH2- and COOH-terminal propeptides of other procollagen types were not found after incubation of endothelial collagen with bacterial collagenase. Additional evidence for the lack of extended noncollagenous sequences was provided by studies with mast cell proteases, which convert native procollagen to collagen but are unreactive toward native interstitial collagens. Endothelial collagen was not cleaved by these enzymes at 37 degrees C, but, as observed for interstitial collagen alpha chains, required prior heating at elevated temperatures for cleavage to occur. In view of this unique set of structural characteristics, and a distribution that is not restricted to the endothelium, we have designated this protein as type VIII collagen.

• Publication year

  1983

• Venue

  Journal of Biological Chemistry

• Publication date

  1983-11-10

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1016/s0021-9258(17)44129-9PMID 6630235
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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