MicroRNA-7a/b Protects against Cardiac Myocyte Injury in Ischemia/Reperfusion by Targeting Poly(ADP-Ribose) Polymerase

Objectives MicroRNA-7 (miR-7) is highly connected to cancerous cell proliferation and metastasis. It is also involved in myocardial ischemia-reperfusion (I/R) injury and is upregulated in cardiomyocyte under simulated I/R (SI/R). We aimed to investigate the role of miR-7 during myocardial I/R injury in vitro and in vivo and a possible gene target. Methods and Results Real-time PCR revealed that miR-7a/b expression was upregulated in H9c2 cells after SI/R. Flow cytometry showed SI/R-induced cell apoptosis was decreased with miR-7a/b mimic transfection but increased with miR-7a/b inhibitor in H9c2 cells. In a rat cardiac I/R injury model, infarct size determination and TUNEL assay revealed that miR-7a/b mimic decreased but miR-7a/b inhibitor increased cardiac infarct size and cardiomyocyte apoptosis as compared with controls. We previously identified an important gene connected with cell apoptosis -- poly(ADP-ribose) polymerase (PARP) -- as a candidate target for miR-7a/b and verified the target by luciferase reporter activity assay and western blot analysis. Conclusions miR-7a/b is sensitive to I/R injury and protects myocardial cells against I/R-induced apoptosis by negatively regulating PARP expression in vivo and in vitro. miR-7a/b may provide a new therapeutic approach for treatment of myocardial I/R injury. Poly(ADP-ribose) polymerase.

• Publication year

  2014

• Venue

  PLoS ONE

• Publication date

  2014-03-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1371/journal.pone.0090096PMID 24594984PMCID 3940823
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• microRNA‐210 negatively regulates LPS‐induced production of proinflammatory cytokines by targeting NF‐κB1 in murine macrophages

  2012cited by this paper
• Alterations in expression of specific microRNAs by combination of 4-HPR and EGCG inhibited growth of human malignant neuroblastoma cells.

  2012cited by this paper
• MicroRNA 21 Inhibits Left Ventricular Remodeling in the Early Phase of Rat Model with Ischemia-reperfusion Injury by Suppressing Cell Apoptosis

  2012cited by this paper
• The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2

  2011cited by this paper
• Usp18 Regulates Epidermal Growth Factor (EGF) Receptor Expression and Cancer Cell Survival via MicroRNA-7*

  2011cited by this paper
• MicroRNA-7 Inhibits the Growth of Human Non-Small Cell Lung Cancer A549 Cells through Targeting BCL-2

  2011cited by this paper
• miR-499 regulates mitochondrial dynamics by targeting calcineurin and dynamin-related protein-1

  2011cited by this paper
• Liposomal Delivery of MicroRNA-7–Expressing Plasmid Overcomes Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor-Resistance in Lung Cancer Cells

  2011cited by this paper
• MiR-204 regulates cardiomyocyte autophagy induced by ischemia-reperfusion through LC3-II

  2011cited by this paper
• MicroRNA-7 targets IGF1R (insulin-like growth factor 1 receptor) in tongue squamous cell carcinoma cells.

  2010cited by this paper
• Synergistic effects of the GATA-4-mediated miR-144/451 cluster in protection against simulated ischemia/reperfusion-induced cardiomyocyte death.

  2010cited by this paper
• Downregulation of microRNA-29 by antisense inhibitors and a PPAR-gamma agonist protects against myocardial ischaemia-reperfusion injury.

  2010cited by this paper
• MicroRNA-494 Targeting Both Proapoptotic and Antiapoptotic Proteins Protects Against Ischemia/Reperfusion-Induced Cardiac Injury

  2010cited by this paper
• MicroRNA-320 Is Involved in the Regulation of Cardiac Ischemia/Reperfusion Injury by Targeting Heat-Shock Protein 20

  2009cited by this paper
• Effects of 3-aminobenzamide on expressions of poly (ADP ribose) polymerase and apoptosis inducing factor in cardiomyocytes of rats with acute myocardial infarction.

  2009cited by this paper
• MicroRNA-1 regulates cardiomyocyte apoptosis by targeting Bcl-2.

  2009cited by this paper
• Downregulation of MiR-199a Derepresses Hypoxia-Inducible Factor-1α and Sirtuin 1 and Recapitulates Hypoxia Preconditioning in Cardiac Myocytes

  2009cited by this paper
• Selective Blockade of MicroRNA Processing by Lin28

  2008cited by this paper
• Taking microRNAs to heart.

  2008cited by this paper
• ERKs/p53 signal transduction pathway is involved in doxorubicin-induced apoptosis in H9c2 cells and cardiomyocytes.

  2008cited by this paper
• Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.

  2008cited by this paper
• MicroRNAs: powerful new regulators of heart disease and provocative therapeutic targets.

  2007cited by this paper
• microRNA functions.

  2007cited by this paper
• Emerging role of microRNAs in cardiovascular biology.

  2007cited by this paper
• The muscle-specific microRNAs miR-1 and miR-133 produce opposing effects on apoptosis by targeting HSP60, HSP70 and caspase-9 in cardiomyocytes

  2007cited by this paper
• Hypoxia/reoxygenation-induced cytotoxicity in cultured human lymphocytes.

  2007cited by this paper
• Control of Stress-Dependent Cardiac Growth and Gene Expression by a MicroRNA

  2007cited by this paper
• Extensive post-transcriptional regulation of microRNAs and its implications for cancer.

  2006cited by this paper
• Bmc Complementary and Alternative Medicine Effect of Curcuma Longa and Ocimum Sanctum on Myocardial Apoptosis in Experimentally Induced Myocardial Ischemic-reperfusion Injury

  2006cited by this paper
• Poly(ADP-ribose) polymerase-1 hyperactivation and impairment of mitochondrial respiratory chain complex I function in reperfused mouse hearts.

  2006cited by this paper
• The Widespread Impact of Mammalian MicroRNAs on mRNA Repression and Evolution

  2005cited by this paper
• MicroRNAs: a developing story.

  2005cited by this paper
• Effects of phytoestrogen genistein on myocardial ischemia/reperfusion injury and apoptosis in rabbits.

  2004influential reference
• Intensified Cloning Efforts Have Revealed Numer

  2004cited by this paper
• MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing.

  2003cited by this paper
• Multiply Attenuated, Self-Inactivating Lentiviral Vectors Efficiently Deliver and Express Genes for Extended Periods of Time in Adult Rat Cardiomyocytes In Vivo

  2003cited by this paper
• ARC inhibits cytochrome c release from mitochondria and protects against hypoxia-induced apoptosis in heart-derived H9c2 cells.

  1999cited by this paper
• Essential contribution of caspase 3/CPP32 to apoptosis and its associated nuclear changes.

  1998cited by this paper
• Caspases and caspase inhibitors.

  1997cited by this paper
• ICE-LAP6, a Novel Member of the ICE/Ced-3 Gene Family, Is Activated by the Cytotoxic T Cell Protease Granzyme B*

  1996cited by this paper

• Ischemic cardiac stromal fibroblast-derived protein mediators in the infarcted myocardium and transcriptomic profiling at single cell resolution

  2024cites this paper
• Hsa-circ-ACSL1 Enhances Apoptosis and Autophagy in Myocarditis Cardiomyocytes Through the miR-7-5p/XBP1 Axis

  2024cites this paper
• Non-coding RNA mediates endoplasmic reticulum stress-induced apoptosis in heart disease

  2023cites this paper
• Sevoflurane up-regulates miR-7a to protect against ischemic brain injury in rats by down-regulating ATG7 and reducing neuronal autophagy.

  2022cites this paper
• The circRNA-miRNA/RBP regulatory network in myocardial infarction

  2022cites this paper
• Diagnostic and Prognostic Value of miRNAs after Coronary Artery Bypass Grafting: A Review

  2021cites this paper
• Long Noncoding RNAs Involved in Cardiomyocyte Apoptosis Triggered by Different Stressors

  2021cites this paper
• MiR-7a-5p Attenuates Hypoxia/Reoxygenation-Induced Cardiomyocyte Apoptosis by Targeting VDAC1

  2021cites this paper
• Circular RNAs in cardiovascular diseases.

  2021cites this paper
• Alternative Splicing in Cardiovascular Disease—A Survey of Recent Findings

  2021influential citation
• MicroRNA-26a/b-5p promotes myocardial infarction-induced cell death by downregulating cytochrome c oxidase 5a

  2021cites this paper
• CircRNAs: role in human diseases and potential use as biomarkers

  2021cites this paper
• Non-coding RNAs in Cardiomyopathy and Heart Failure

  2021cites this paper
• WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA

  2021cites this paper
• Curcumin prevents brain damage and cognitive dysfunction during ischemic-reperfusion through the regulation of miR-7-5p.

  2020cites this paper
• Deep Insights in Circular RNAs: from biogenesis to therapeutics

  2020cites this paper
• Critical functions of microRNA‐30a‐5p‐E2F3 in cardiomyocyte apoptosis induced by hypoxia/reoxygenation

  2020cites this paper
• Circular RNAs and cancer: Opportunities and challenges.

  2020cites this paper
• Protective Effect of Pravastatin on Myocardial Ischemia Reperfusion Injury by Regulation of the miR-93/Nrf2/ARE Signal Pathway

  2020cites this paper
• microRNAs Alterations of Myocardium and Brain Ischemia-Reperfusion Injury: Insight to Improve Infarction

  2020cites this paper
• Icariside II protects cardiomyocytes from hypoxia-induced injury by upregulating the miR-7-5p/BTG2 axis and activating the PI3K/Akt signaling pathway

  2020cites this paper
• Biogenesis, Features, Functions, and Disease Relationships of a Specific Circular RNA: CDR1as

  2020cites this paper
• Regulatory Roles of Circular RNAs in Coronary Artery Disease

  2020cites this paper
• Interactions Among Regulatory Non-coding RNAs Involved in Cardiovascular Diseases.

  2020cites this paper
• Circular RNA-Centered Regulatory Networks in the Physiopathology of Cardiovascular Diseases

  2020cites this paper
• Small Molecules with Big Impacts on Cardiovascular Diseases

  2020cites this paper
• Long noncoding RNA-MEG3 contributes to myocardial ischemia–reperfusion injury through suppression of miR-7-5p expression

  2019cites this paper
• The Role of Poly(ADP-Ribose) Polymerase-1 in Cutaneous Wound Healing

  2019cites this paper
• Overexpression of miRNA-410-3p protects hypoxia-induced cardiomyocyte injury via targeting TRAF5.

  2019cites this paper
• The administration of dexmedetomidine changes microRNA expression profiling of rat hearts.

  2019cites this paper
• A novel strategy of identifying circRNA biomarkers in cardiovascular disease by meta‐analysis

  2019cites this paper
• Implication of serum microRNA-7a/B with myocardial cell injury induced by ischemia reperfusion

  2018cites this paper
• miR-7-5p overexpression suppresses cell proliferation and promotes apoptosis through inhibiting the ability of DNA damage repair of PARP-1 and BRCA1 in TK6 cells exposed to hydroquinone.

  2018cites this paper
• MicroRNA-208a Regulates H9c2 Cells Simulated Ischemia-Reperfusion Myocardial Injury via Targeting CHD9 through Notch/NF-kappa B Signal Pathways.

  2018cites this paper
• A comprehensive review of circRNA: from purification and identification to disease marker potential

  2018cites this paper
• Novel Roles of Non-Coding RNAs in Opioid Signaling and Cardioprotection

  2018influential citation
• Ischemic postconditioning confers cerebroprotection by stabilizing VDACs after brain ischemia

  2018cites this paper
• CircRNA: as a disease marker potential and research strategy

  2018cites this paper
• Dissecting Pathogenetic Mechanisms and Therapeutic Strategies in Drosophila Models of Myotonic Dystrophy Type 1

  2018cites this paper
• Comparison of Cardiac miRNA Transcriptomes Induced by Diabetes and Rapamycin Treatment and Identification of a Rapamycin-Associated Cardiac MicroRNA Signature

  2018cites this paper
• Mir-23a inhibition attenuates ischemic/reperfusion-induced myocardial apoptosis by targeting XIAP.

  2017cites this paper
• MiR-146 b protects cardiomyocytes injury in myocardial ischemia / reperfusion by targeting Smad 4

  2017influential citation
• The novel roles of circular RNAs in metabolic organs

  2017cites this paper
• Circular RNAs in Cardiovascular Disease: An Overview

  2017cites this paper
• Circadian MicroRNAs in Cardioprotection.

  2017cites this paper
• MiR-146b protects cardiomyocytes injury in myocardial ischemia/reperfusion by targeting Smad4.

  2017cites this paper
• Uric acid promotes cardiomyocyte apoptosis through regulating microRNA-21 / PTEN / AKT / eNOS axis in myocardial ischemia / reperfusion injury

  2017cites this paper
• 1,25(OH)2D3 improves cardiac dysfunction, hypertrophy, and fibrosis through PARP1/SIRT1/mTOR‐related mechanisms in type 1 diabetes

  2017cites this paper
• Effects of miRNAs on myocardial apoptosis by modulating mitochondria related proteins

  2017cites this paper
• Rho-kinase signaling pathway promotes the expression of PARP to accelerate cardiomyocyte apoptosis in ischemia/reperfusion

  2017influential citation
• The Circular RNA Cdr1as Promotes Myocardial Infarction by Mediating the Regulation of miR-7a on Its Target Genes Expression

  2016influential citation
• Circular RNAs: Novel rising stars in cardiovascular disease research.

  2016cites this paper
• δ-Opioid Receptor Activation and MicroRNA Expression in the Rat Heart Under Prolonged Hypoxia

  2016cites this paper
• MicroRNA-93 inhibits ischemia-reperfusion induced cardiomyocyte apoptosis by targeting PTEN

  2016influential citation
• miR-7a/b attenuates post-myocardial infarction remodeling and protects H9c2 cardiomyoblast against hypoxia-induced apoptosis involving Sp1 and PARP-1

  2016cites this paper
• Overview of MicroRNAs in Cardiac Hypertrophy, Fibrosis, and Apoptosis

  2016cites this paper
• Protective effect of microRNA-30b on hypoxia/reoxygenation-induced apoptosis in H9C2 cardiomyocytes.

  2015cites this paper
• Sp1 Mediates a Therapeutic Role of MiR-7a/b in Angiotensin II-Induced Cardiac Fibrosis via Mechanism Involving the TGF-β and MAPKs Pathways in Cardiac Fibroblasts

  2015cites this paper
• MicroRNAs in myocardial infarction

  2015cites this paper
• Luteolin Inhibits Ischemia/Reperfusion-Induced Myocardial Injury in Rats via Downregulation of microRNA-208b-3p

  2015cites this paper
• Bone marrow-derived mesenchymal stem cells rescue injured H9c2 cells via transferring intact mitochondria through tunneling nanotubes in an in vitro simulated ischemia/reperfusion model

  2015cites this paper
• miRNA signature of unfolded protein response in H9c2 rat cardiomyoblasts

  2014cites this paper
• The Protective Effect of MicroRNA-320 on Left Ventricular Remodeling after Myocardial Ischemia-Reperfusion Injury in the Rat Model

  2014cites this paper