SCIENCE@home

An isotope tracer study of glucose catabolism pathways in liver.

C. Wenner,S. Weinhouse

Published 1956 in Journal of Biological Chemistry

ABSTRACT

Recognition of addit,ional pathways of glucose dissimilation via pentose (l-3) has led to considerable interest in the extent to which the pentose route participates in the metabolism of glucose in cells. Though enzymes involved in this process have been found widely dist.ributed in nature (4-c), the ext,ent of glucose breakdown t’hrough the pcntose pathway, as compared to t.hat & the classical glycolytic process of Embden and Meyerhof, still remains uncertain. Thus far, most of the published estimates have been based on the relative incorporat,ion of CL4 from variously C”-labeled glucoses into respiratory CO, (7-Y). Although this procedure is capable of providing qualitative evidence of the occurrence of t.he pent.ose pathway under cert.ain circumstances, its application to animal tissue thus far has led to results, the quantitative interpretation of which appears to be in dispute (8). A detailed discussion of the shortcomings of this means of estimation is available (10). Recently a method has been employed in our laboratory which, we believe, provides a more direct, and therefore possibly more reliable, estimation of the occurrence of these processes in cells. Reports of its application to yeast (11) and to a variety of microorganisms and neoplastic and non-neoplastic animal tissue (12) have been published. Though the application of this procedure to animal tissue is still under study, the uncertainty of estimat.ions based on CO2 measurement has prompted us to submit our own findings at this time. The principle of our method has been previously described (11, 12). Experiment,ally, it. involves the simultaneous cat,abolism of carbon l-labeled and uniformly labeled glucose by aliquots of cells in vitro (slices and whole homogenates of animal tissue have been used), followed by isolation and Cl4 assay of a 2or 3-carbon intermediate such as pyruvatc, lactate, or acetate, or a substance directly derived therefrom such as cholesterol, fatty acids, acetoacetate, etc. If there was no endogenous metabolism, 3-carbon

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-15 of 15 references · Page 1 of 1

CITED BY

Showing 1-100 of 136 citing papers · Page 1 of 2

12
Next