Protein Kinase CK2α′ Is Induced by Serum as a Delayed Early Gene and Cooperates with Ha-ras in Fibroblast Transformation*

Protein kinase CK2 is an ubiquitous and pleiotropic Ser/Thr protein kinase composed of two catalytic (α and/or α′) and two noncatalytic (β) subunits forming a heterotetrameric holoenzyme involved in cell growth and differentiation. Here we report the identification, cloning, and oncogenic activity of the murine CK2α′ subunit. Serum treatment of quiescent mouse fibroblasts induces CK2α′ mRNA expression, which peaks at 4 h. The kinetics ofCK2α′ expression correlate with increased kinase activity toward a specific CK2 holoenzyme peptide substrate. The ectopic expression of CK2α′ (or CK2α) cooperates with Ha-ras in foci formation of rat primary embryo fibroblasts. Moreover, we observed that BALB/c 3T3 fibroblasts transformed with Ha-ras and CK2α′ show a faster growth rate than cells transformed with Ha-rasalone. In these cells the higher growth rate correlates with an increase in calmodulin phosphorylation, a protein substrate specifically affected by isolated CK2 catalytic subunits but not by CK2 holoenzyme, suggesting that unbalanced expression of a CK2 catalytic subunit synergizes with Ha-ras in cell transformation.

• Publication year

  1998

• Venue

  Journal of Biological Chemistry

• Publication date

  1998-08-14

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.273.33.21291PMID 9694889
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• On the physiological role of casein kinase II in Saccharomyces cerevisiae.

  1998cited by this paper
• Temperature-sensitive Mutations of the CKA1 Gene Reveal a Role for Casein Kinase II in Maintenance of Cell Polarity inSaccharomyces cerevisiae *

  1998cited by this paper
• Mutational analysis of residues implicated in the interaction between protein kinase CK2 and peptide substrates.

  1997cited by this paper
• Regulation of protein phosphatase 2A by direct interaction with casein kinase 2alpha.

  1997cited by this paper
• Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation.

  1997cited by this paper
• Tal‐1 induces T cell acute lymphoblastic leukemia accelerated by casein kinase IIalpha.

  1996cited by this paper
• Identification of a c-fos-induced gene that is related to the platelet-derived growth factor/vascular endothelial growth factor family.

  1996cited by this paper
• Cloning, expression and properties of the alpha' subunit of casein kinase 2 from zebrafish (Danio rerio).

  1996cited by this paper
• Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation

  1995cited by this paper
• Casein kinase II alpha transgene-induced murine lymphoma: relation to theileriosis in cattle

  1995cited by this paper
• Casein kinase II in theileriosis

  1995cited by this paper
• A new platelet-derived growth factor-regulated genomic element which binds a serine/threonine phosphoprotein mediates induction of the slow immediate-early gene MCP-1

  1995cited by this paper
• Casein Kinase II Is Required for Cell Cycle Progression during G1 and G2/M in Saccharomyces cerevisiae(*)

  1995cited by this paper
• Casein kinase 2 down-regulation and activation by polybasic peptides are mediated by acidic residues in the 55-64 region of the beta-subunit. A study with calmodulin as phosphorylatable substrate.

  1994cited by this paper
• Regulation of casein kinase II by growth factors: a reevaluation.

  1994cited by this paper
• Casein kinase II is required for transition of G0/G1, early G1, and G1/S phases of the cell cycle.

  1994cited by this paper
• Recombinant human casein kinase II. A study with the complete set of subunits (alpha, alpha' and beta), site-directed autophosphorylation mutants and a bicistronically expressed holoenzyme.

  1994cited by this paper
• Recombinant human casein kinase II

  1994cited by this paper
• Substrate specificity of protein kinase CK2.

  1994cited by this paper
• Evidence for the induction of casein kinase II in bovine lymphocytes transformed by the intracellular protozoan parasite Theileria parva.

  1993cited by this paper
• Casein kinases: pleiotropic mediators of cellular regulation.

  1993cited by this paper
• Activation of mammalian DNA ligase I through phosphorylation by casein kinase II.

  1992cited by this paper
• Casein kinase II phosphorylates the eukaryote‐specific C‐terminal domain of topoisomerase II in vivo.

  1992cited by this paper
• Casein kinase II phosphorylation increases the rate of serum response factor‐binding site exchange.

  1992cited by this paper
• Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.

  1992cited by this paper
• Yeast GCN4 as a probe for oncogenesis by AP-1 transcription factors: transcriptional activation through AP-1 sites is not sufficient for cellular transformation.

  1992cited by this paper
• Phosphorylation of casein kinase II by p34cdc2 in vitro and at mitosis.

  1992cited by this paper
• Cloning and characterization of the casein kinase II alpha subunit gene from the lymphocyte-transforming intracellular protozoan parasite Theileria parva.

  1992cited by this paper
• Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.

  1991cited by this paper
• Phosphorylation of transcriptional factors and cell-cycle-dependent proteins by casein kinase II.

  1991cited by this paper
• Myb DNA binding inhibited by phosphorylation at a site deleted during oncogenic activation

  1990cited by this paper
• Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II.

  1990cited by this paper
• The p53 tumour suppressor protein is phosphorylated at serine 389 by casein kinase II.

  1990cited by this paper
• Casein kinase 2: an 'eminence grise' in cellular regulation?

  1990cited by this paper
• Molecular cloning of the human casein kinase II alpha subunit.

  1989cited by this paper
• Myc oncoproteins are phosphorylated by casein kinase II.

  1989cited by this paper
• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

  1987cited by this paper
• An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

  1987cited by this paper
• Induction of c-fos gene and protein by growth factors precedes activation of c-myc

  1984cited by this paper
• Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.

  1983cited by this paper
• Isolation of phosphorylated peptides and proteins on ion exchange papers.

  1978cited by this paper

• Discovery of KDX1381, a Bivalent CK2α Inhibitor for the Treatment of Solid Tumors as a Single Agent or in Combination.

  2025cites this paper
• Regulation of Mouse CK2α (Csnk2a1) Promoter Expression In Vitro and in Cell Lines

  2025cites this paper
• Circadian Control of Protein Synthesis

  2024cites this paper
• Protein Kinase CK2α’, More than a Backup of CK2α

  2023cites this paper
• Casein Kinase 2 (CK2): A Possible Therapeutic Target in Acute Myeloid Leukemia

  2023cites this paper
• CK2-induced cooperation of HHEX with the YAP-TEAD4 complex promotes colorectal tumorigenesis

  2022cites this paper
• Protein kinase CK2: a potential therapeutic target for diverse human diseases

  2021cites this paper
• How can a traffic light properly work if it is always green? The paradox of CK2 signaling

  2021influential citation
• Binding of ellagic acid and urolithin metabolites to the CK2 protein, based on the ONIOM method and molecular docking calculations

  2019cites this paper
• Up-Regulation of the Alpha Prime Subunit of Protein Kinase CK2 as a Marker of Fast Proliferation in GL261 Cultured Cells

  2019cites this paper
• Design And Assessment of Small Molecules as Free Radical Scavengers and Potential Multi-Target Therapeutic Agents against Alzheimer’s Disease, Diabetes and Cancer

  2018cites this paper
• The multifunctional role of phospho-calmodulin in pathophysiological processes

  2018cites this paper
• Casein Kinase 2 Is Linked to Stress Granule Dynamics through Phosphorylation of the Stress Granule Nucleating Protein G3BP1

  2016cites this paper
• Misregulation of the proline rich homeodomain (PRH/HHEX) protein in cancer cells and its consequences for tumour growth and invasion

  2016cites this paper
• Systematic investigation of hierarchical phosphorylation by protein kinase CK2.

  2015cites this paper
• Identification of Ellagic acid analogues as potent inhibitor of protein Kinase CK2:A chemopreventive role in oral Cancer

  2015cites this paper
• QSAR study of CK2 inhibitors by GA-MLR and GA-SVM methods

  2015cites this paper
• Investigating the interplay between protein kinases and caspases

  2013cites this paper
• Characterizing the convergence of protein kinase CK2 and caspase-3 reveals isoform-specific phosphorylation of caspase-3 by CK2α′: implications for pathological roles of CK2 in promoting cancer cell survival

  2013cites this paper
• CK2 in Embryonic Development

  2013cites this paper
• WITHDRAWN: QSAR and pharmacophore modeling of some benzimidazole derivatives as Protein Kinase CK2 Inhibitors

  2013cites this paper
• Nuclear Extracellular Signal-Regulated Kinase 1 and 2 Translocation Is Mediated by Casein Kinase 2 and Accelerated by Autophosphorylation

  2011cites this paper
• Structure‐Based Pharmacophore Modeling from Multicomplex: a Comprehensive Pharmacophore Generation of Protein Kinase CK2 and Virtual Screening Based on it for Novel Inhibitors

  2011cites this paper
• Urolithin as a Converging Scaffold Linking Ellagic acid and Coumarin Analogues: Design of Potent Protein Kinase CK2 Inhibitors

  2011cites this paper
• Study on the Anticancer Activity of Coumarin Derivatives by Molecular Modeling

  2011cites this paper
• Casein Kinase 2 Regulates the mRNA-destabilizing Activity of Tristetraprolin*

  2011cites this paper
• Lack of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is accompanied by increased CK2α′ levels

  2011cites this paper
• CK2α is essential for embryonic morphogenesis

  2011cites this paper
• Protein kinase CK 2 signal in neoplasia

  2010cites this paper
• Characterization and optimization of antigen-specific T cell responses during ex vivo expansion of melanoma tumor-infiltrating lymphocytes

  2010cites this paper
• The role of post-translation regulation of Twist expression in the tumor progression of squamous cell carcinoma of head and neck

  2010cites this paper
• Mitotic Regulation of Protein Kinase CK2

  2010cites this paper
• CK2 Modulation of NF-κB, TP53, and the Malignant Phenotype in Head and Neck Cancer by Anti-CK2 Oligonucleotides In vitro or In vivo via Sub–50-nm Nanocapsules

  2010cites this paper
• The p53-p21Cip1/WAF1 pathway is necessary for cellular senescence induced by the inhibition of protein kinase CKII in human colon cancer cells

  2009cites this paper
• Evidence for Regulation of Mitotic Progression through Temporal Phosphorylation and Dephosphorylation of CK2α

  2009cites this paper
• Protein Kinase CK2: a major regulator of the G1/S transition in Saccharomyces cerevisiae

  2009cites this paper
• Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library

  2009cites this paper
• Silencing of the CKII alpha and CKII alpha' genes during cellular senescence is mediated by DNA methylation.

  2009cites this paper
• Coumarin as attractive casein kinase 2 (CK2) inhibitor scaffold: an integrate approach to elucidate the putative binding motif and explain structure-activity relationships.

  2008cites this paper
• KIF5C: A new binding partner for protein kinase CK2 with a preference for the CK2α’ subunit

  2008cites this paper
• The regulation of RNA polymerase III transcription by protein kinase CK2

  2008cites this paper
• Too much of a good thing: the role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2.

  2008cites this paper
• Protein kinase CK2, an important regulator of the inflammatory response?

  2008cites this paper
• Protein kinase CK2 as a druggable target.

  2008cites this paper
• Protein kinase CK2 catalyzes tyrosine phosphorylation in mammalian cells.

  2008cites this paper
• Protein Kinase CK2α as an Unfavorable Prognostic Marker and Novel Therapeutic Target in Acute Myeloid Leukemia

  2007cites this paper
• Pharmacological inhibition of protein kinase CK2 reverts the multidrug resistance phenotype of a CEM cell line characterized by high CK2 level

  2007cites this paper
• Inhibition of Margination and Diapedesis of Neutrophils by Protein Synthesis Blockade

  2007cites this paper
• Phosphorylation of CKBBP2/CRIF1 by protein kinase CKII promotes cell proliferation.

  2007cites this paper
• Tetrabromocinnamic Acid (TBCA) and Related Compounds Represent a New Class of Specific Protein Kinase CK2 Inhibitors

  2007cites this paper
• Protein kinase CK2alpha as an unfavorable prognostic marker and novel therapeutic target in acute myeloid leukemia.

  2007cites this paper
• Downregulation of protein kinase CKII is associated with cellular senescence

  2006cites this paper
• Evaluation of 3-carboxy-4(1H)-quinolones as inhibitors of human protein kinase CK2.

  2006cites this paper
• Protein kinase casein kinase 2 mediates inhibitor-kappaB kinase and aberrant nuclear factor-kappaB activation by serum factor(s) in head and neck squamous carcinoma cells.

  2006cites this paper
• Differential Expression of Skin Cancer and Hair-Follicle Cycle Regulated Genes in Tumor Susceptible K14-Agouti Mice

  2006cites this paper
• Identification of ellagic acid as potent inhibitor of protein kinase CK2: a successful example of a virtual screening application.

  2006cites this paper
• Insight into the complex genetic network of tetraploid Atlantic salmon (Salmo salar L.): description of multiple novel Pax-7 splice variants.

  2006cites this paper
• Factor ( s ) in Head and Neck Squamous Carcinoma Cells B Activation by Serum κ Kinase and Aberrant Nuclear Factor-B κ Protein Kinase Casein Kinase 2 Mediates Inhibitor-Updated

  2006cites this paper
• Tetrabromobenzotriazole (TBBt) and tetrabromobenzimidazole (TBBz) as selective inhibitors of protein kinase CK2: evaluation of their effects on cells and different molecular forms of human CK2.

  2005cites this paper
• Differential expression of genes encoding protein kinase CK2 subunits in the plant cell cycle.

  2005cites this paper
• The Multiple Personalities of the Regulatory Subunit of Protein Kinase CK2: CK2 Dependent and CK2 Independent Roles Reveal a Secret Identity for CK2β

  2005cites this paper
• B23 is a downstream target of polyamine- modulated CK2

  2005cites this paper
• CK2 inhibits apoptosis and changes its cellular localization following ionizing radiation.

  2005cites this paper
• Expression of the gene for hematopoietic cell specific protein is not restricted to cells of hematopoietic origin.

  2005cites this paper
• Inspecting the structure-activity relationship of protein kinase CK2 inhibitors derived from tetrabromo-benzimidazole.

  2005cites this paper
• Validation of protein kinase CK2 as oncological target

  2005cites this paper
• 2-Dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole: a novel powerful and selective inhibitor of protein kinase CK2.

  2004cites this paper
• Mitogenic stimulation of transcription by RNA polymerase III.

  2004cites this paper
• Optimization of protein kinase CK2 inhibitors derived from 4,5,6,7-tetrabromobenzimidazole.

  2004cites this paper
• Contribution of RasGRP1 to BCR-induced deletion in the immature B cell line WEHI 231

  2004cites this paper
• Inhibition of protein kinase CK2 by condensed polyphenolic derivatives. An in vitro and in vivo study.

  2004cites this paper
• Order or chaos? An evaluation of the regulation of protein kinase CK2.

  2004cites this paper
• Casein Kinase II Alpha Subunit and C1-Inhibitor Are Independent Predictors of Outcome in Patients with Squamous Cell Carcinoma of the Lung

  2004cites this paper
• Protein kinase CKIIa interacts with the Bcr moiety of Bcr/Abl and mediates proliferation of Bcr/Abl-expressing cells

  2004cites this paper
• Inhibition of Protein Kinase CK 2 by Condensed Polyphenolic Derivatives . An in Vitro and in Vivo Study †

  2004cites this paper
• Casein Kinase II Alpha Subunit and C 1-Inhibitor Are Independent Predictors of Outcome in Patients with Squamous Cell Carcinoma of the Lung

  2004cites this paper
• Phosphorylation of Threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by Protein Kinase CKII Promotes the Degradation of IκBα and p27Kip1*

  2003cites this paper
• The protein of a new gene, Tctex4, interacts with protein kinase CK2beta subunit and is highly expressed in mouse testis.

  2003cites this paper
• Protein kinase CKIIα interacts with the Bcr moiety of Bcr/Abl and mediates proliferation of Bcr/Abl-expressing cells

  2003cites this paper
• Tyrosine phosphorylation of protein kinase CK2 by Src-related tyrosine kinases correlates with increased catalytic activity.

  2003cites this paper
• Protein kinase CK2: structure, regulation and role in cellular decisions of life and death.

  2003cites this paper
• Spermatogenesis of mice lacking CK2α′: Failure of germ cell survival and characteristic modifications of the spermatid nucleus

  2003cites this paper
• Inhibition of Protein Kinase CK2 by Anthraquinone-related Compounds

  2003cites this paper
• The genes encoding human protein kinase CK2 and their functional links.

  2003cites this paper
• The raison d'être of constitutively active protein kinases: the lesson of CK2.

  2003cites this paper
• A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2.

  2003cites this paper
• Phosphorylation of recombinant human spermidine/spermine N(1)-acetyltransferase by CK1 and modulation of its binding to mitochondria: a comparison with CK2.

  2002cites this paper
• Transcription in Human Cells and Activates RNA Polymerase III CK 2 Forms a Stable Complex with TFIIIB

  2002cites this paper
• Activation of protein kinase CK2 is an early step in the ultraviolet B-mediated increase in interstitial collagenase (matrix metalloproteinase-1; MMP-1) and stromelysin-1 (MMP-3) protein levels in human dermal fibroblasts.

  2002cites this paper
• Protein kinase CK2 promotes aberrant activation of nuclear factor-kappaB, transformed phenotype, and survival of breast cancer cells.

  2002cites this paper
• CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation.

  2002cites this paper
• Inhibitory effect and its kinetic analysis of tyrphostin AG1478 on recombinant human protein kinase CK2 holoenzyme.

  2002cites this paper
• Unique Activation Mechanism of Protein Kinase CK2

  2002cites this paper
• Protein Kinase CK 2 Promotes Aberrant Activation of Nuclear Factor-B , Transformed Phenotype , and Survival of Breast Cancer Cells 1

  2002cites this paper
• Protein Kinase CK2 Promotes Aberrant Activation of Nuclear Factor-κB, Transformed Phenotype, and Survival of Breast Cancer Cells

  2002cites this paper
• Phosphorylation regulates the stability of the regulatory CK2β subunit

  2002cites this paper
• CK2 Forms a Stable Complex with TFIIIB and Activates RNA Polymerase III Transcription in Human Cells

  2002cites this paper
• Protein kinase CK2 inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) induces apoptosis and caspase-dependent degradation of haematopoietic lineage cell-specific protein 1 (HS1) in Jurkat cells.

  2002cites this paper
• Toward the rational design of protein kinase casein kinase-2 inhibitors.

  2002cites this paper
• CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with β-TrCP and enhances β-catenin degradation

  2002cites this paper