Structure of Hepatitis C Virus Polymerase in Complex with Primer-Template RNA

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Journal of Virology

• Publication date

  2012-04-11

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1128/JVI.00386-12PMID 22496223PMCID PMC3393583
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Nucleoside, nucleotide, and non-nucleoside inhibitors of hepatitis C virus NS5B RNA-dependent RNA-polymerase.

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  2011influential reference
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• Use of thermal melt curves to assess the quality of enzyme preparations.

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• Gene Composer: database software for protein construct design, codon engineering, and gene synthesis

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• Phaser crystallographic software

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• Correspondence e-mail:

  2000cited by this paper
• Crystal structure of the RNA-dependent RNA polymerase of hepatitis C virus.

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  1999cited by this paper
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  1998cited by this paper
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  1998cited by this paper
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  1997cited by this paper
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  1996cited by this paper

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