Continuous fluorescence monitoring of rapid cycle DNA amplification. 1997.

Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green I, (ii) a decrease i...

• Publication year

  2013

• Venue

  BioTechniques

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.2144/000114043PMID 23905170
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

  1997cited by this paper
• Cy7PE and Cy7APC: bright new probes for immunofluorescence.

  1996cited by this paper
• Molecular Beacons: Probes that Fluoresce upon Hybridization

  1996cited by this paper
• Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.

  1995cited by this paper
• Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater.

  1995cited by this paper
• PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.

  1994cited by this paper
• Resonance energy transfer: methods and applications.

  1994cited by this paper
• Rapid cycle sequencing in an air thermal cycler.

  1993cited by this paper
• Effect of heat denaturation of target DNA on the PCR amplification.

  1993cited by this paper
• Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

  1993cited by this paper
• Allelic discrimination by nick-translation PCR with fluorogenic probes.

  1993cited by this paper
• A method for accurate amplification of polymorphic CA-repeat sequences.

  1993cited by this paper
• Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus.

  1993cited by this paper
• Simultaneous Amplification and Detection of Specific DNA Sequences

  1992cited by this paper
• Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.

  1991cited by this paper
• Rapid cycle DNA amplification: time and temperature optimization.

  1991cited by this paper
• Minimizing the time required for DNA amplification by efficient heat transfer to small samples.

  1990cited by this paper
• Cyanine dye labeling reagents containing isothiocyanate groups.

  1989cited by this paper
• Automated polymerase chain reaction in capillary tubes with hot air.

  1989cited by this paper
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

  1988cited by this paper
• Large- and small-scale phenol extractions.

  1987cited by this paper

• Evaluation of Reference Gene Stability in Goat Skeletal Muscle Satellite Cells during Proliferation and Differentiation Phases

  2024cites this paper
• Rapid and Sensitive Human-Specific DNA Quantitation Using a Microfluidic Amplification Module at the Point-of-Care

  2023cites this paper
• Leaf PIP2.1 and PIP2.5 are down-regulated in ‘Mandarin’ lime under drought

  2022cites this paper
• Screening of Stably Expressed Internal Reference Genes for Quantitative Real-Time PCR Analysis in Quail

  2022cites this paper
• DCD-chip designed for the digital and ultraprecise quantification of copy number variation.

  2022cites this paper
• Evaluation of candidate reference genes for quantitative real-time PCR analysis in a male rat model of dietary iron deficiency

  2021cites this paper
• Microbial Ecology Metrics to Assess the Effect of Biocide on Souring Control and Improve Souring Modelling

  2021cites this paper
• Establishment of a rapid detection method for wheat crown rot

  2021cites this paper
• Modernizing the Toolkit for Arthropod Bloodmeal Identification

  2021cites this paper
• So Many Diagnostic Tests, So Little Time: Review and Preview of Candida auris Testing in Clinical and Public Health Laboratories

  2021cites this paper
• PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

  2020cites this paper
• Quantitative Assessment of Parental Somatic Mosaicism for Copy‐Number Variant (CNV) Deletions

  2020cites this paper
• Modern Tools for Detection and Diagnosis of Plant Pathogens

  2020cites this paper
• Oncogenic Viruses and Their Molecular Diagnosis in Poultry

  2020influential citation
• Histidine-conjugated DNA as a biomolecular depot for metal ions

  2020cites this paper
• Reactivity of antimony(V) and its effect on the pro-inflammatory response in the RAW 264.7 monocyte/macrophage cell line

  2020cites this paper
• Identification of Reference Genes for Quantitative Gene Expression Studies in Three Tissues of Japanese Quail

  2019cites this paper
• A novel emulsion PCR method coupled with fluorescence spectrophotometry for simultaneous qualitative, quantitative and high-throughput detection of multiple DNA targets

  2019cites this paper
• Molecular Monitoring of Chronic Myeloid Leukemia.

  2019cites this paper
• Revealing the secrets of PCR

  2019cites this paper
• Molecular and New-Generation Techniques for Rapid Detection of Foodborne Pathogens and Characterization of Microbial Communities in Poultry Meat

  2019cites this paper
• A rapid and accurate methylation‐sensitive high‐resolution melting analysis assay for the diagnosis of Prader Willi and Angelman patients

  2019cites this paper
• Clinical and Molecular Diagnosis in Muscular Dystrophies

  2019cites this paper
• Application of Molecular and Sequencing Techniques in Analysis of Microbial Diversity in Agroecosystem

  2019cites this paper
• Simultaneous single-channel multiplex and quantification of carbapenem-resistant genes using multidimensional standard curves

  2018cites this paper
• The Function of G-Protein-Coupled Receptor-Regulatory Cascade in Southern House Mosquitoes (Diptera: Culicidae)

  2018cites this paper
• Use of a Purpose-Built Impression Cytology Device for Gene Expression Quantification at the Ocular Surface Using Quantitative PCR and Droplet Digital PCR

  2018cites this paper
• Confocal epifluorescence sensor with an arc-shaped aperture for slide-based PCR quantification.

  2018cites this paper
• Universally applicable, quantitative PCR method utilizing fluorescent nucleobase analogs

  2018cites this paper
• Experimental Approaches in Omic Sciences

  2018cites this paper
• Effects of PCR inhibitors on mRNA expression for human blood identification.

  2018cites this paper
• A one-step ultrasonic irradiation assisted strategy for the preparation of polymer-functionalized carbon quantum dots and their biological imaging.

  2018cites this paper
• A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids

  2018cites this paper
• Duplex fluorescence melting curve analysis as a new tool for rapid detection and differentiation of genotype I, II and Bartha-K61 vaccine strains of pseudorabies virus

  2018cites this paper
• Laboratory Approaches in Molecular Pathology—The Polymerase Chain Reaction

  2017cites this paper
• A high-throughput analysis method of microdroplet PCR coupled with fluorescence spectrophotometry

  2017cites this paper
• Essential Concepts and Techniques in Molecular Biology

  2017cites this paper
• Molecular Technologies for the Detection and Characterisation of Food‐Borne Pathogens

  2017cites this paper
• Analysis of differentially expressed proteins between the spinetoram-susceptible and -resistant strains of Plutella xylostella (L.)

  2017cites this paper
• Assessing Fungal Biodiversity Using Molecular Markers

  2017cites this paper
• Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers.

  2017cites this paper
• Adaptive PCR Based on Hybridization Sensing of Mirror-Image l-DNA.

  2017cites this paper
• Rapid identification of Bacillus anthracis by real-time PCR with dual hybridization probes in environmental swabs.

  2017cites this paper
• Chapter 5 References

  2017cites this paper
• Regulation of P450-mediated permethrin resistance in Culex quinquefasciatus by the GPCR/Gαs/AC/cAMP/PKA signaling cascade

  2017cites this paper
• Sperm sexing in Nili-Ravi buffalo through modified swim up: Validation using SYBR® green real-time PCR.

  2017cites this paper
• Clinical Manifestations, Treatment, and Diagnosis of Tropheryma whipplei Infections

  2017cites this paper
• PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement.

  2017cites this paper
• A novel quantitative PCR mediated by high-fidelity DNA polymerase

  2017cites this paper
• High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays.

  2017cites this paper
• Rapid and sensitive detection of salmonid alphavirus using TaqMan real-time PCR.

  2017cites this paper
• PKB/Akt kinase localization and role in stemness maintenance in cancer

  2016cites this paper
• Availability: A Metric for Nucleic Acid Strand Displacement Systems

  2016cites this paper
• MnO2 nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

  2016cites this paper
• The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression.

  2016cites this paper
• Nucleic Acid Amplification Methods Overview

  2016cites this paper
• Basic Rules in Nucleic Acid-Mediated Amplification and Hybridization Methods in Food Safety Detection: A Review

  2016cites this paper
• Plant Virus Detection and Diagnosis: Progress and Challenges

  2016cites this paper
• An International Inter-Laboratory Digital PCR Study Demonstrates High Reproducibility for the Measurement of a Rare Sequence Variant

  2016cites this paper
• Molecular Prospecting: Advancement in Diagnosis and Control of Rhizoctonia solani Diseases in Plants

  2016cites this paper
• Molecular Diagnosis of Killer Pathogen of Potato: Phytophthora infestans and Its Management

  2016cites this paper
• Fluorescent Labeling of Plasmid DNA and mRNA: Gains and Losses of Current Labeling Strategies.

  2016cites this paper
• Poisonous or non-poisonous plants? DNA-based tools and applications for accurate identification

  2016cites this paper
• Detection and Quantification of Bremia lactucae by Spore Trapping and Quantitative PCR.

  2016cites this paper
• On‐chip quantitative PCR using integrated real‐time detection by capillary electrophoresis

  2016cites this paper
• Synergistic effect of Trichoderma-derived antifungal metabolites and cell wall degrading enzymes on enhanced biocontrol of Fusarium oxysporum f. sp. cucumerinum

  2016cites this paper
• Use of LightCycler mutation analysis to detect type II adenine phosphoribosyltransferase deficiency in two patients with 2,8-dihydroxyadeninuria

  2016cites this paper
• Quantitative Real-Time PCR: Recent Advances.

  2016cites this paper
• Molecular amplification methods in diagnostic virology

  2016cites this paper
• Molecular characterization of Staphylococcus aureus isolates causing skin and soft tissue infections in patients from Malakand, Pakistan

  2016cites this paper
• The source of SYBR green master mix determines outcome of nucleic acid amplification reactions

  2016cites this paper
• Real-time RT-PCR systems for CTC detection from blood samples of breast cancer and gynaecological tumour patients (Review).

  2016cites this paper
• Wire-guided droplet manipulation for molecular biology

  2016cites this paper
• Detection of Dengue Virus in Mosquito Extracts and Human Clinical Samples Using a Field Expedient Molecular Platform.

  2015cites this paper
• Real-time PCR detection chemistry.

  2015cites this paper
• Development and application of a quantitative loop-mediated isothermal amplification method for detecting genetically modified maize MON863.

  2015cites this paper
• Real-Time PCR Approaches for Analysis of Hydrocarbon-Degrading Bacterial Communities

  2015cites this paper
• Gene expression analysis for prostate cancer management.

  2015cites this paper
• Design of primers and probes for quantitative real-time PCR methods.

  2015cites this paper
• Molecular Genetic Analysis of Wetland Soils

  2015cites this paper
• Thyroid and Parathyroid Tumors

  2015cites this paper
• Multivalent aptamer-RNA based fluorescent probes for carrier-free detection of cellular microRNA-34a in mucin1-expressing cancer cells.

  2015cites this paper
• High-resolution assessment of copy number variation.

  2015cites this paper
• Fluorescence-based real-time quantitative polymerase chain reaction (qPCR) technologies for high throughput screening of pathogens

  2015cites this paper
• How to use molecular biology tools for the study of the anaerobic digestion process?

  2015cites this paper
• Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.

  2015cites this paper
• Identification by real-time PCR with SYBR Green of Leishmania spp. and Serratia marcescens in canine 'sterile' cutaneous nodular lesions.

  2015cites this paper
• Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes

  2014cites this paper
• The Pcr Revolution: Basic Technologies And Applications

  2014cites this paper
• Influence of trypsinization and alternative procedures for cell preparation before RNA extraction on RNA integrity.

  2014cites this paper
• Rapid bacterial colonization of low-density polyethylene microplastics in coastal sediment microcosms

  2014cites this paper
• Single-channel multiplexing without melting curve analysis in real-time PCR

  2014cites this paper
• Closed-tube human leukocyte antigen DQA1∗05 genotyping assay based on switchable lanthanide luminescence probes.

  2014cites this paper
• Mediator probe PCR: detection of real-time PCR by label-free probes and a universal fluorogenic reporter.

  2014cites this paper
• Application of PCR and real-time PCR for monitoring cyanobacteria, Microcystis spp. and Cylindrospermopsis raciborskii in Macau freshwater reservoir

  2014cites this paper
• Development of SYBR Green based real time PCR assay for detection of monodon baculovirus in Penaeus monodon.

  2014cites this paper
• Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis

  2014cites this paper
• Melting curve analysis for mutations of EGFR and KRAS.

  2014cites this paper
• Dual-force aggregation of magnetic particles enhances label-free quantification of DNA at the sub-single cell level.

  2014cites this paper
• High-resolution melting analysis: a promising molecular method for meat traceability

  2014cites this paper