In vitro models for the evaluation of angiogenic potential in bone engineering

Blood vessels have a fundamental role both in skeletal homeostasis and in bone repair. Angiogenesis is also important for a successful bone engineering. Therefore, scaffolds should be tested for their ability to favour endothelial cell adhesion, proliferation and functions. The type of endothelial cell to use for in vitro assays should be carefully considered, because the properties of these cells may depend on their source. Morphological and functional relationships between endothelial cells and osteoblasts are evaluated with co-cultures, but this model should still be standardized, particularly for distinguishing the two cell types. Platelet-rich plasma and recombinant growth factors may be useful for stimulating angiogenesis.

• Publication year

  2011

• Venue

  Acta Pharmacologica Sinica

• Publication date

  Unknown publication date

• Fields of study

  Medicine, Chemistry, Engineering

• Identifiers
  DOI 10.1038/aps.2010.143PMID 21042285PMCID 4003307
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Regeneration and Healing

  2012cited by this paper
• Cross-Linking of Gelatin and Chitosan Complex Nanofibers for Tissue-Engineering Scaffolds

  2011cited by this paper
• A porous scaffold for bone tissue engineering/45S5 Bioglass® derived porous scaffolds for co-culturing osteoblasts and endothelial cells

  2010cited by this paper
• Sonic Hedgehog Promotes Angiogenesis and Osteogenesis in a Coculture System Consisting of Primary Osteoblasts and Outgrowth Endothelial Cells

  2010cited by this paper
• αvβ3 Integrin Limits the Contribution of Neuropilin-1 to Vascular Endothelial Growth Factor-induced Angiogenesis*

  2009cited by this paper
• Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases.

  2009cited by this paper
• The Use of Endothelial Progenitor Cells for Prevascularized Bone Tissue Engineering

  2009cited by this paper
• Growth and phenotypic expression of human endothelial cells cultured on a glass-reinforced hydroxyapatite

  2009cited by this paper
• Proteases and receptors in the recruitment of endothelial progenitor cells in neovascularization.

  2009cited by this paper
• A review of the current research on the role of bFGF and VEGF in angiogenesis.

  2009cited by this paper
• Crosstalk between osteoblasts and endothelial cells co-cultured on a polycaprolactone-starch scaffold and the in vitro development of vascularization.

  2009cited by this paper
• Vascular integrity mediated by vascular endothelial cadherin and regulated by sphingosine 1-phosphate and angiopoietin-1.

  2009cited by this paper
• Role of vascular endothelial growth factor in the communication between human osteoprogenitors and endothelial cells

  2009cited by this paper
• Vascular progenitor cells and translational research: the role of endothelial and smooth muscle progenitor cells in endogenous arterial remodelling in the adult.

  2009cited by this paper
• Cell-to-cell communication between osteogenic and endothelial lineages: implications for tissue engineering.

  2009cited by this paper
• Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells.

  2009cited by this paper
• Endothelial cells incubated with platelet‐rich plasma express PDGF‐B and ICAM‐1 and induce bone marrow stromal cell migration

  2009cited by this paper
• Osteoblasts stimulated with pulsed electromagnetic fields increase HUVEC proliferation via a VEGF‐A independent mechanism

  2009cited by this paper
• Role of platelet-derived growth factors in physiology and medicine.

  2008cited by this paper
• Interleukin-3 promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation.

  2008cited by this paper
• The effect of human osteoblasts on proliferation and neo-vessel formation of human umbilical vein endothelial cells in a long-term 3D co-culture on polyurethane scaffolds.

  2008cited by this paper
• Vascularization in tissue engineering.

  2008cited by this paper
• Bone remodeling during fracture repair: The cellular picture.

  2008cited by this paper
• Analysis of the Biological Response of Endothelial and Fibroblast Cells Cultured on Synthetic Scaffolds with Various Hydrophilic/Hydrophobic Ratios: Influence of Fibronectin Adsorption and Conformation

  2008cited by this paper
• Spatial and temporal patterns of bone formation in ectopically pre-fabricated, autologous cell-based engineered bone flaps in rabbits

  2008cited by this paper
• Osteogenesis and angiogenesis: the potential for engineering bone.

  2008cited by this paper
• Induction of angiogenesis in tissue-engineered scaffolds designed for bone repair: A combined gene therapy–cell transplantation approach

  2008cited by this paper
• Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels.

  2008cited by this paper
• Evaluation of VEGF‐mediated signaling in primary human cells reveals a paracrine action for VEGF in osteoblast‐mediated crosstalk to endothelial cells

  2008cited by this paper
• Promotion of osteogenesis in tissue‐engineered bone by pre‐seeding endothelial progenitor cells‐derived endothelial cells

  2008cited by this paper
• Biocompatibility studies of endothelial cells on a novel calcium phosphate/SiO2-xerogel composite for bone tissue engineering

  2008cited by this paper
• Circulating endothelial/skeletal progenitor cells for bone regeneration and healing.

  2008cited by this paper
• The hepatocyte growth factor/c-Met signaling pathway as a therapeutic target to inhibit angiogenesis.

  2008cited by this paper
• The Interleukin-8 Pathway in Cancer

  2008cited by this paper
• Interaction between human umbilical vein endothelial cells and human osteoprogenitors triggers pleiotropic effect that may support osteoblastic function.

  2008cited by this paper
• Expression of cell adhesion receptors in human osteoblasts cultured on biofunctionalized poly-(epsilon-caprolactone) surfaces.

  2007cited by this paper
• Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science

  2007cited by this paper
• Downloaded from jdr.sagepub.com at PENNSYLVANIA STATE UNIV on February 23, 2013 For personal use only. No other uses without permission. International and American Associations for Dental ResearchCRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE VEGF: an Essent

  2007cited by this paper
• Hedgehog Signaling

  2007cited by this paper
• Tissue-like self-assembly in cocultures of endothelial cells and osteoblasts and the formation of microcapillary-like structures on three-dimensional porous biomaterials.

  2007cited by this paper
• Human endothelial cell growth and phenotypic expression on three dimensional poly(lactide‐co‐glycolide) sintered microsphere scaffolds for bone tissue engineering

  2007cited by this paper
• Expression of cell adhesion receptors in human osteoblasts cultured on biofunctionalized poly-(ε-caprolactone) surfaces

  2007cited by this paper
• Gravity Spun Polycaprolactone Fibers for Applications in Vascular Tissue Engineering: Proliferation and Function of Human Vascular Endothelial Cells

  2006cited by this paper
• Therapeutic potential of vasculogenesis and osteogenesis promoted by peripheral blood CD34-positive cells for functional bone healing.

  2006cited by this paper
• Maintenance of the hematopoietic stem cell pool by CXCL12-CXCR4 chemokine signaling in bone marrow stromal cell niches.

  2006cited by this paper
• Gene Expression Profiling Reveals Platelet-Derived Growth Factor Receptor Alpha as a Target of Cell Contact-Dependent Gene Regulation in an Endothelial Cell–Osteoblast Co-culture Model

  2006cited by this paper
• Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry

  2006cited by this paper
• Reduced expression of endothelial connexins 43 and 37 in hypertensive rats is rectified after 7-day carvedilol treatment.

  2006cited by this paper
• Eph receptor and ephrin ligand-mediated interactions during angiogenesis and tumor progression.

  2006cited by this paper
• Recombinant human granulocyte–colony‐stimulating factor–mobilized and apheresis‐collected endothelial progenitor cells: a novel blood cell component for therapeutic vasculogenesis

  2006cited by this paper
• Mobilization of Bone Marrow‐Derived Hematopoietic and Endothelial Stem Cells After Orthotopic Liver Transplantation and Liver Resection

  2006cited by this paper
• Vascular Endothelial Growth Factor-D Activates VEGFR-3 Expressed in Osteoblasts Inducing Their Differentiation*

  2006cited by this paper
• Endothelial Cells Assemble into a 3-Dimensional Prevascular Network in a Bone Tissue Engineering Construct

  2006cited by this paper
• The effect of irradiation modification and RGD sequence adsorption on the response of human osteoblasts to polycaprolactone.

  2005cited by this paper
• Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry

  2005cited by this paper
• VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice.

  2005cited by this paper
• VEGF-activated angiogenesis during bone regeneration.

  2005cited by this paper
• Angiogenesis Is Required for Successful Bone Induction During Distraction Osteogenesis

  2005cited by this paper
• Human Primary Endothelial Cells Stimulate Human Osteoprogenitor Cell Differentiation

  2004cited by this paper
• Functional significance of Tie2 signaling in the adult vasculature.

  2004cited by this paper
• Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of human endothelial cells.

  2004cited by this paper
• Tissue engineering: Creation of long-lasting blood vessels

  2004cited by this paper
• Human microvascular endothelial cells immortalized with human telomerase catalytic protein: a model for the study of in vitro angiogenesis.

  2004cited by this paper
• Characterization of Two Types of Endothelial Progenitor Cells and Their Different Contributions to Neovasculogenesis

  2004cited by this paper
• Platelet-released supernatant increases matrix metalloproteinase-2 production, migration, proliferation, and tube formation of human umbilical vascular endothelial cells.

  2004cited by this paper
• Modulation of In Vitro Angiogenesis in a Three-Dimensional Spheroidal Coculture Model for Bone Tissue Engineering

  2004cited by this paper
• Functionalization of poly-L-lactic-co-ε-caprolactone: effects of surface modification on endothelial cell proliferation and hemocompatibility

  2003cited by this paper
• Diverse Origin and Function of Cells With Endothelial Phenotype Obtained From Adult Human Blood

  2003cited by this paper
• Effects of locally applied vascular endothelial growth factor (VEGF) and VEGF‐inhibitor to the rabbit tibia during distraction osteogenesis

  2003cited by this paper
• Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration

  2003cited by this paper
• Hedgehog stimulates only osteoblastic differentiation of undifferentiated KS483 cells.

  2003cited by this paper
• Ex vivo gene therapy in autologous bone marrow stromal stem cells for tissue-engineered maxillofacial bone regeneration

  2003cited by this paper
• Endothelin-1 promotes osteoprogenitor proliferation and differentiation in fetal rat calvarial cell cultures.

  2003cited by this paper
• The biology of VEGF and its receptors

  2003cited by this paper
• Functionalization of poly-(L-lactic-co-epsilon-caprolactone): effects of surface modification on endothelial cell proliferation and hemocompatibility [corrected].

  2003cited by this paper
• Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres.

  2003cited by this paper
• Role of Angiopoietins and Tie receptor tyrosine kinases in angiogenesis and lymphangiogenesis

  2003cited by this paper
• Vascular endothelial growth factor up-regulates expression of receptor activator of NF-kappa B (RANK) in endothelial cells. Concomitant increase of angiogenic responses to RANK ligand.

  2003cited by this paper
• Vascular Endothelial Growth Factor Up-regulates Expression of Receptor Activator of NF-κB (RANK) in Endothelial Cells

  2003cited by this paper
• Effects of FGF-2 on metaphyseal fracture repair in rabbit tibiae

  2003cited by this paper
• Comparison of the effects of 17alpha-ethinylestradiol and 17beta-estradiol on the proliferation of human breast cancer cells and human umbilical vein endothelial cells.

  2002cited by this paper
• Relationships between trabecular bone remodeling and bone vascularization: a quantitative study.

  2002cited by this paper
• Determination of bone marrow-derived endothelial progenitor cell significance in angiogenic growth factor-induced neovascularization in vivo.

  2002cited by this paper
• Effect of HUVEC on human osteoprogenitor cell differentiation needs heterotypic gap junction communication.

  2002cited by this paper
• Endothelial Progenitor Cell Vascular Endothelial Growth Factor Gene Transfer for Vascular Regeneration

  2002cited by this paper
• In vitro expression of the endothelial phenotype: comparative study of primary isolated cells and cell lines, including the novel cell line HPMEC-ST1.6R.

  2002cited by this paper
• Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells: implications for fracture healing.

  2002cited by this paper
• Software-supported image quantification of angiogenesis in an in vitro culture system: application to studies of biocompatibility.

  2002cited by this paper
• Generation of Human Pulmonary Microvascular Endothelial Cell Lines

  2001cited by this paper
• Evaluation of endothelial cell integrins after in vitro contact with polyethylene terephthalate

  2001cited by this paper
• Tissue specific regulation of VEGF expression during bone development requires Cbfa1/Runx2.

  2001cited by this paper
• Neovascularization of ischemic myocardium by human bone-marrow–derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function

  2001cited by this paper
• Receptor Activator of NF-κB and Osteoprotegerin Expression by Human Microvascular Endothelial Cells, Regulation by Inflammatory Cytokines, and Role in Human Osteoclastogenesis*

  2001cited by this paper
• Polymeric system for dual growth factor delivery

  2001cited by this paper
• Decreased bone anabolic effect of basic fibroblast growth factor at fatty marrow sites in ovariectomized rats.

  2001cited by this paper
• Interleukin-6 expression by cultured human endothelial cells in contact with carbon coated polyethylene terephthalate

  2001cited by this paper
• The morphogen Sonic hedgehog is an indirect angiogenic agent upregulating two families of angiogenic growth factors

  2001cited by this paper
• Vascular endothelial growth factor (VEGF) directly enhances osteoclastic bone resorption and survival of mature osteoclasts

  2000cited by this paper
• Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen.

  2000cited by this paper
• Biomaterial-microvasculature interactions.

  2000cited by this paper

• Cellular Crosstalk Between Periodontal Ligament Fibroblasts and Macrophages: Insights from 2D and 3D Culture Systems in External Inflammatory Root Resorption.

  2025cites this paper
• 3D Multicellular Scaffold Based Model for Advancing Bone Disorder Research

  2025cites this paper
• Strategies in Electrospun Polymer and Hybrid Scaffolds for Enhanced Cell Integration and Vascularization for Bone Tissue Engineering and Organoids.

  2024cites this paper
• 3D geometry orchestrates the transcriptional landscape of metastatic neuroblastoma cells in a multicellular in vitro bone model

  2023cites this paper
• A Comprehensive Look at In Vitro Angiogenesis Image Analysis Software

  2023cites this paper
• THE EFFECT OF ADVANCED GLYCATION END PRODUCT OF HUMAN UMBILICAL CORD MESENCHYMAL STEM CELLS ON VASCULAR ENDOTHELIAL GROWTH FACTOR

  2021cites this paper
• Mechanical and cellular characterization of electrospun poly(l-lactic acid)/gelatin yarns with potential as angiogenesis scaffolds

  2021cites this paper
• Bone Regeneration, Reconstruction and Use of Osteogenic Cells; from Basic Knowledge, Animal Models to Clinical Trials

  2020cites this paper
• Evolving applications of the egg: chorioallantoic membrane assay and ex vivo organotypic culture of materials for bone tissue engineering

  2020cites this paper
• A review of in vitro cell culture testing methods for bioactive glasses and other biomaterials for hard tissue regeneration.

  2020cites this paper
• CO-CULTURE SYSTEMS OF OSTEOBLASTS AND OSTEOCLASTS: SIMULATING IN VITRO BONE REMODELING IN REGENERATIVE APPROACHES.

  2020cites this paper
• Multilayer nanoscale functionalization to treat disorders and enhance regeneration of bone tissue.

  2019cites this paper
• Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts

  2019cites this paper
• From the Clinical Problem to the Basic Research—Co-Culture Models of Osteoblasts and Osteoclasts

  2018cites this paper
• Customizable biomaterials as tools for advanced anti-angiogenic drug discovery.

  2018cites this paper
• The effect of platelet rich fibrin in oral surgery: a literature review

  2017cites this paper
• TLR4 stimulation by LPS enhances angiogenesis in a co‐culture system consisting of primary human osteoblasts and outgrowth endothelial cells

  2017cites this paper
• Investigation of mesenchymal stem cell response to bioactive nanotopography

  2016cites this paper
• Quantification of angiogenic sprouting under different growth factors in a microfluidic platform.

  2016cites this paper
• The influence of platelet-derived products on angiogenesis and tissue repair: a concise update

  2015cites this paper
• Transparent, Nanoporous, and Transferable Membrane‐Based Cell–Cell Paracrine Signaling Assay

  2015cites this paper
• Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix.

  2015cites this paper
• Controlled Release of Bioactive Molecules from Collagen-Based Scaffolds for Bone Repair

  2014cites this paper
• In vitro microenvironments to study breast cancer bone colonisation.

  2014cites this paper
• In vitro und in vivo Analyse von Mono- und Ko-Kultur-Sphäroiden zur potentiellen Anwendung im Knochen-Tissue Engineering

  2014cites this paper
• Three-dimensional osteogenic and chondrogenic systems to model osteochondral physiology and degenerative joint diseases

  2014cites this paper
• Cell/Tissue Microenvironment Engineering and Monitoring in Tissue Engineering, Regenerative Medicine, and In Vitro Tissue Models

  2014cites this paper
• Tuan degenerative joint diseases Three-dimensional osteogenic and chondrogenic systems to model osteochondral physiology and

  2014cites this paper
• Characterization of Silk Fibroin Modified Surface: A Proteomic View of Cellular Response Proteins Induced by Biomaterials

  2014cites this paper
• Amphiphilic degradable polymers for immobilization and sustained delivery of sphingosine 1-phosphate.

  2014cites this paper
• Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part I: Recapitulation of Native Tissue Healing and Variables for the Design of Delivery Systems

  2013cites this paper
• L’ostéoprotégérine, nouvel acteur dans l’angiogenèse : Rôle dans la formation de nouveaux vaisseaux et mécanisme d’action

  2013cites this paper
• Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

  2013cites this paper
• Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect.

  2013cites this paper
• Characterization of the Mechanical and Biological Properties of a New Alumina Scaffold

  2013cites this paper
• Assessing the responses of cellular proteins induced by hyaluronic acid-modified surfaces utilizing a mass spectrometry-based profiling system: over-expression of CD36, CD44, CDK9, and PP2A.

  2012cites this paper
• Blood vessel formation in the tissue-engineered bone with the constitutively active form of HIF-1α mediated BMSCs.

  2012influential citation
• Osteoblasts and their applications in bone tissue engineering

  2012cites this paper
• Effect of hyperglycaemia on VEGF-A splice variants, junctional integrity and vascular leakage in human feto-placental vessels from normal and type 1 diabetic pregnancies

  2012cites this paper
• Photocrosslinkable dextran hydrogel films as substrates for osteoblast and endothelial cell growth

  2012cites this paper