Angiotensin II but not potassium induces subcellular redistribution of protein kinase C in bovine adrenal glomerulosa cells.

The distribution of calcium-activated, phospholipid-dependent protein kinase (protein kinase C) between cytosol and membrane fractions was examined in bovine adrenal glomerulosa cells treated with angiotensin II or potassium. Protein kinase C was isolated from cytosol and from detergent-solubilized particulate fractions by DEAE-cellulose chromatography. A major peak of activity for both the soluble and particulate forms of adrenal glomerulosa protein kinase C was eluted at 0.05-0.09 M NaCl. The soluble and particulate forms were found to constitute about 95 and 5%, respectively, of the total enzyme activity in unstimulated cells. A second peak of kinase activity was eluted with 0.15-0.19 M NaCl, which was not dependent on the presence of phospholipids. Exposure of isolated cells for 20 min to 10(-8) M angiotensin II resulted in a decrease in cytosolic activity to 30-40% of control values, and in a corresponding increase in protein kinase C activity associated with the particulate fraction. This hormone-induced redistribution was found to be dose-dependent with an ED50 of 2 nM for angiotensin II, and it occurred rapidly, reaching a plateau within 5-10 min. It was prevented by the specific antagonist [Sar1,Ala8]angiotensin II. By contrast, stimulation with 12 mM KCl did not change the subcellular distribution of protein kinase C activity. These results suggest that redistribution of protein kinase C represents an early step in the post-receptor activation cascade following angiotensin II, but not potassium stimulation of adrenal glomerulosa cells.

• Publication year

  1987

• Venue

  Journal of Biological Chemistry

• Publication date

  1987-06-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)47524-2PMID 3597361
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Intracellular calcium and adenosine 3',5'-cyclic monophosphate as mediators of potassium-induced aldosterone secretion.

  1985cited by this paper
• Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs.

  1985cited by this paper
• Phospholipid-sensitive Ca2+-dependent protein kinase: a major protein phosphorylation system.

  1984cited by this paper
• Correlation between cytosolic free Ca2+ and aldosterone production in bovine adrenal glomerulosa cells. Evidence for a difference in the mode of action of angiotensin II and potassium.

  1984cited by this paper
• The effects of extracellular K+ and angiotensin II on cytosolic Ca++ and steroidogenesis in adrenal glomerulosa cells.

  1984cited by this paper
• The temporal integration of the aldosterone secretory response to angiotensin occurs via two intracellular pathways.

  1984cited by this paper
• Hormonal regulation of a calcium-activated, phospholipid-dependent protein kinase in bovine adrenal cortex.

  1984cited by this paper
• Inositol trisphosphate and diacylglycerol as second messengers.

  1984cited by this paper
• Phospholipid turnover in hormone action.

  1984cited by this paper
• Phospholipid-sensitive Ca2+-dependent protein phosphorylation system in various types of leukemic cells from human patients and in human leukemic cell lines HL60 and K562, and its inhibition by alkyl-lysophospholipid.

  1983cited by this paper
• Decrease in cytosolic calcium/phospholipid-dependent protein kinase activity following phorbol ester treatment of EL4 thymoma cells.

  1982cited by this paper
• The role of cyclic AMP in aldosterone production by isolated zona glomerulosa cells.

  1979cited by this paper
• Studies on cyclic nucleotides in the adrenal gland. VIII. Effects of angiotensin on adenosine 3',5'-monophosphate and steroidogenesis in the adrenal cortex.

  1978cited by this paper
• Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. II. Proenzyme and its activation by calcium-dependent protease from rat brain.

  1977cited by this paper
• Studies on a cyclic nucleotide-independent protein kinase and its proenzyme in mammalian tissues. I. Purification and characterization of an active enzyme from bovine cerebellum.

  1977cited by this paper
• Aldosterone production by isolated adrenal glomerulosa cells: stimulation by physiological concentrations of angiotensin II.

  1975cited by this paper
• In vitro effects of ACTH, angiotensins, serotonin and potassium on steroid output and conversion of corticosterone to aldosterone by isolated adrenal cells.

  1970cited by this paper

• Steroidogenesis-adrenal cell signal transduction.

  2014cites this paper
• Regulation of aldosterone synthesis and secretion.

  2014cites this paper
• Acute and chronic regulation of aldosterone production.

  2012cites this paper
• ACTH PKC-in Induced Various Cortisol Secretion Is Mediated by cAMP Adrenocortical Adenomas and

  2006cites this paper
• ACTH and Phorbol Protein Kinase C in Adenoma

  2006cites this paper
• The control by angiotensin II of cholesterol supply for aldosterone biosynthesis.

  2004cites this paper
• Angiotensin and Aldosterone Biosynthesis

  2004cites this paper
• Control of aldosterone secretion: a model for convergence in cellular signaling pathways.

  2004cites this paper
• Angiotensin II activates cholesterol ester hydrolase in bovine adrenal glomerulosa cells through phosphorylation mediated by p42/p44 mitogen-activated protein kinase.

  2003cites this paper
• Control of Calcium Homeostasis by Angiotensin II in Adrenal Glomerulosa Cells through Activation of p38 MAPK*

  2002cites this paper
• Elevated K(+) induces myristoylated alanine-rich C-kinase substrate phosphorylation and phospholipase D activation in glomerulosa cells.

  2001cites this paper
• Age dependence of signal transduction and cell signaling as a major factor of intervention into the aging process.

  2001cites this paper
• Steroidogenic acute regulatory (StAR) protein in bovine adrenal steroidogenesis

  2001cites this paper
• Angiotensin II and calcium channels.

  2000cites this paper
• Changes in the cytosolic Ca2+ concentration and Ca2+‐sensitivity of the contractile apparatus during angiotensin II‐induced desensitization in the rabbit femoral artery

  2000influential citation
• Aldosterone

  1999cites this paper
• Effects of angiotensin II and adrenocorticotropic hormone on myristoylated alanine-rich C-kinase substrate phosphorylation in glomerulosa cells.

  1999cites this paper
• Regulation of Adrenocortical Function by Vasopressin

  1998cites this paper
• Angiotensin II potentiates adrenocorticotrophic hormone-induced cAMP formation in bovine adrenal glomerulosa cells through a capacitative calcium influx.

  1998cites this paper
• ACTH-induced cortisol secretion is mediated by cAMP and PKC in various adrenocortical adenomas.

  1997cites this paper
• Generation of the catalytic fragment of protein kinase C alpha in spastic canine basilar artery.

  1997cites this paper
• Demonstration of an angiotensin II-induced negative feedback effect on aldosterone synthesis in isolated rat adrenal zona glomerulosa cells.

  1996cites this paper
• Calcium Stimulates Intramitochondrial Cholesterol Transfer in Bovine Adrenal Glomerulosa Cells*

  1996cites this paper
• Angiotensinogen gene activation by angiotensin II is mediated by the rel A (nuclear factor-kappaB p65) transcription factor: one mechanism for the renin angiotensin system positive feedback loop in hepatocytes.

  1996influential citation
• Regulation of norepinephrine transport system by angiotensin II in neuronal cultures of normotensive and spontaneously hypertensive rat brains.

  1996cites this paper
• Mechanisms for inducible control of angiotensinogen gene transcription.

  1996cites this paper
• The site of action of Ca2+ in the activation of steroidogenesis: studies in Ca(2+)-clamped bovine adrenal zona-glomerulosa cells.

  1995cites this paper
• Tissue Renin-Angiotensin Systems

  1995cites this paper
• Potassium‐angiotensin interplay in the regulation of aldosterone biosynthesis

  1995cites this paper
• AT1 receptors and angiotensin actions in the brain and neuronal cultures of normotensive and hypertensive rats.

  1995cites this paper
• Comparison of protein phosphorylation patterns produced in adrenal cells by activation of cAMP-dependent protein kinase and Ca-dependent protein kinase.

  1995cites this paper
• Diacylglycerol production, Ca2+ influx, and protein kinase C activation in sustained cellular responses.

  1995cites this paper
• Comparative effects of a highly specific protein kinase C inhibitor, calphostin C and calmodulin inhibitors on angiotensin-stimulated aldosterone secretion.

  1994cites this paper
• Role of calcium and other mediators in aldosterone secretion from the adrenal glomerulosa cells.

  1994cites this paper
• ACTH and phorbol ester stimulated redistribution of protein kinase C in human cortisol-producing adrenal adenoma.

  1994cites this paper
• Receptor-mediated effects of angiotensin II on neurons.

  1994cites this paper
• Role of specific isoforms of protein kinase C in angiotensin II and lipoxygenase action in rat adrenal glomerulosa cells.

  1994cites this paper
• Calcium-mediated intracellular acidification and activation of Na(+)-H+ exchange in adrenal glomerulosa cells stimulated with potassium.

  1993cites this paper
• Early and late effects of angiotensin-II on Ca2+ fluxes in bovine adrenal zona glomerulosa cells.

  1993cites this paper
• Thapsigargin inhibits voltage-activated calcium channels in adrenal glomerulosa cells.

  1993cites this paper
• Mechanism of angiotensin II-induced proliferation in bovine adrenocortical cells.

  1992cites this paper
• Atrial natriuretic peptide-induced inhibition of aldosterone secretion: a quest for mediator(s)

  1992cites this paper
• The role of protein kinase-C in control of aldosterone production by rat adrenal glomerulosa cells: activation of protein kinase-C by stimulation with potassium.

  1992cites this paper
• The roles of protein-tyrosine kinases in neutrophil and airway smooth muscle cell activation

  1992cites this paper
• Stimulation of early gene expression by angiotensin II in bovine adrenal glomerulosa cells: roles of calcium and protein kinase C.

  1992cites this paper
• Greater importance of Ca(2+)-calmodulin in maintenance of ang II- and K(+)-mediated aldosterone secretion: lesser role of protein kinase C.

  1992cites this paper
• Inhibition of human neutrophil responses by α‐cyano‐3,4‐dihydroxythiocinnamamide; a protein‐tyrosine kinase inhibitor

  1992cites this paper
• Signal transduction mechanisms involved in carbachol-induced aldosterone secretion from bovine adrenal glomerulosa cells.

  1992cites this paper
• Inhibition of human neutrophil responses by alpha-cyano-3,4-dihydroxythiocinnamamide; a protein-tyrosine kinase inhibitor.

  1992cites this paper
• Direct evidence for a key role of protein kinase C in the development of vasospasm after subarachnoid hemorrhage.

  1992cites this paper
• Studies on the intracellular mechanism of action of alpha-melanocyte-stimulating hormone on rat adrenal zona glomerulosa.

  1992cites this paper
• Characterization of calcium-independent forms of protein kinase C-beta in phorbol ester-treated rabbit platelets.

  1991cites this paper
• Angiotensin receptor-mediated stimulation of diacylglycerol production in pulmonary artery endothelial cells.

  1991cites this paper
• Angiotensin-II activation of cAMP and corticosterone production in bovine adrenocortical cells: effects of nonpeptide angiotensin-II antagonists.

  1991cites this paper
• [Transmembrane signal. Respective role of free cytosol calcium and of protein kinase C].

  1991cites this paper
• Evidence that angiotensin II enhances noradrenaline release from sympathetic nerves in mouse atria by activating protein kinase C.

  1991cites this paper
• Possible involvement of protein kinase C in the development of raphe neurons in primary culture

  1991cites this paper
• Angiotensin-II-induced changes in diacylglycerol levels and their potential role in modulating the steroidogenic response.

  1991cites this paper
• Phorbol ester, prolactin, and relaxin cause translocation of protein kinase C from cytosol to membranes in human endometrial cells.

  1991cites this paper
• Expression of protein kinase C isoforms in various steroidogenic cell types.

  1991cites this paper
• Control of steroidogenesis by the calcium messenger system in human adrenocortical cells.

  1991cites this paper
• ACTH-induced inhibition of the action of angiotensin II in bovine zona glomerulosa cells. A modulatory effect of cyclic AMP on the angiotensin II receptor.

  1991cites this paper
• Angiotensin II decreases cGMP levels in neuronal cultures from rat brain.

  1991cites this paper
• Angiotensin II-induced activation of Na(+)-H+ exchange in adrenal glomerulosa cells is mediated by protein kinase C.

  1991cites this paper
• Generation and role of calcium signal in adrenal glomerulosa cells

  1991cites this paper
• Involvement of protein kinase C in the coupling between the V1 vasopressin receptor and phospholipase C in rat glomerulosa cells: effects on aldosterone secretion.

  1991cites this paper
• The major endogenous bovine brain protein kinase C inhibitor is a heat‐labile protein

  1991cites this paper
• Contrasting effects of sn-1,2-dioctanoyl glycerol as compared to other protein kinase C activators in adrenal glomerulosa cells.

  1991cites this paper
• Intracellular mediators of potassium-induced aldosterone secretion.

  1990cites this paper
• Protein kinase C activation by platelet-activating factor is independent of enzyme translocation.

  1990cites this paper
• sn-1,2 dioctanoylglycerol mimics the effects of angiotensin II on aldosterone production and potassium permeability in isolated bovine glomerulosa cells.

  1990cites this paper
• Role of protein kinase C on the steroidogenic effect of angiotensin II in the rat adrenal glomerulosa cell.

  1990cites this paper
• Effects of atrial natriuretic factor on angiotensin-II-and phorbol ester-stimulated protein kinase-C and prostacyclin production in cultured rat aortic smooth muscle cells.

  1990cites this paper
• Trypsin stimulation of aldosterone and 18-hydroxycorticosterone production by rat adrenal zona glomerulosa tissue is mediated by activation of protein kinase C.

  1990cites this paper
• A potential role for phospholipase-D in the angiotensin-II-induced stimulation of aldosterone secretion from bovine adrenal glomerulosa cells.

  1990cites this paper
• Protein Kinase C Activity in Rat Brain Cortex

  1990cites this paper
• [18-Hydroxycorticosterone, 18-hydroxydesoxycorticosterone. Why, when, how to determine plasma levels in some adrenal pathologies].

  1990cites this paper
• Regulation of 1,2-diacylglycerol production by angiotensin-II in bovine adrenal glomerulosa cells.

  1990cites this paper
• Evidence that angiotensin II decreases mitochondrial calcium in the glomerulosa cell.

  1990cites this paper
• Calcium as intracellular messenger: from simplicity to complexity.

  1990cites this paper
• Physiological and pharmacological comparisons of angiotensin II receptors in neuronal and astrocyte glial cultures.

  1990cites this paper
• Mode of action of angiotensin II and vasopressin on their target cells.

  1990cites this paper
• Cytosolic free calcium oscillates in single bovine adrenal glomerulosa cells in response to angiotensin II stimulation.

  1989cites this paper
• Role of calcium in angiotensin II-mediated aldosterone secretion.

  1989cites this paper
• Angiotensin II increases diacylglycerol in calf adrenal glomerulosa cells by activating de novo phospholipid synthesis.

  1989cites this paper
• Selective inhibition of angiotensin-II-mediated aldosterone secretion by 5-hydroxyeicosatetraenoic acid.

  1989cites this paper
• Effects of angiotensin II and of phorbol ester on protein kinase C activity and on prostacyclin production in cultured rat aortic smooth-muscle cells.

  1989cites this paper
• Cholinergic agonists prime the beta-cell to glucose stimulation.

  1989cites this paper
• Specific effects of agonists of the calcium messenger system on secretion of 'late-pathway' steroid products by intact tissue and dispersed cells of the rat adrenal zona glomerulosa.

  1989cites this paper
• Protein kinase C mediates the effect of vasopressin in pituitary corticotrophs.

  1989cites this paper
• Stimulation of Formation of Inositol Phosphates in Primary Cultures of Bovine Adrenal Chromaffin Cells by Angiotensin II, Histamine, Bradykinin, and Carbachol

  1988cites this paper
• A spatial-temporal model of cell activation.

  1988cites this paper
• Calcium stimulates steroidogenesis in permeabilized bovine adrenal cortical cells.

  1988cites this paper
• Angiotensin II stimulates vimentin phosphorylation via a Ca2+-dependent, protein kinase C-independent mechanism in cultured vascular smooth muscle cells.

  1988cites this paper
• Stimulation of cortisol production in isolated bovine zona fasciculata cells by phorbol ester: role of ion fluxes.

  1988cites this paper
• Subcellular distribution of protein kinase C in rat adrenal glomerulosa cells.

  1988cites this paper
• Protein kinase-C in the human fetal adrenal gland.

  1988cites this paper
• Inositol trisphosphate isomers in angiotensin II-stimulated adrenal glomerulosa cells.

  1988cites this paper
• Stimulus-secretion coupling in angiotensin-stimulated adrenal glomerulosa cells.

  1988cites this paper
• Differential redistribution of protein kinase C in human aldosteronoma cells and adjacent adrenal cells stimulated with ACTH and angiotensin II.

  1988cites this paper