RNAi Screening for Fat Regulatory Genes with SRS Microscopy

Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.

• Publication year

  2010

• Venue

  Nature Methods

• Publication date

  2010-12-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.1556PMID 21240281PMCID 3061290
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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