Direct determination of acetyl-enzyme intermediate in the acetylcholinesterase-catalyzed hydrolysis of acetylcholine and acetylthiocholine.

Acetylcholinesterase from Electrophorus electricus was acetylated during the hydrolysis of [3H]acetylcholine and [3H]acetylthiocholine. The steady state levels of [3H]acetyl-enzyme were measured at different pH and different concentrations of substrate. The maximum acetylation fraction [S)----infinity) at pH 7.0 in 0.5 M salt was 0.65 with acetylcholine as substrate and 0.57 with acetylthiocholine as substrate. Acetylation is faster than deacetylation. The fraction of acetyl-enzyme was not affected by pH which indicates that acetylation and deacetylation are equally affected by changes in pH. This results supports the concept that acetylation and deacetylation involve similar mechanisms.

• Publication year

  1984

• Venue

  Journal of Biological Chemistry

• Publication date

  1984-09-10

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0021-9258(18)90614-9PMID 6469995
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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