Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.

Stoichiometric amounts of MgATP disassemble dephosphorylated smooth muscle and nonmuscle myosin filaments to a 10 S monomer. Phosphorylation of the regulatory light chain reassembles the myosin into filaments (Suzuki, H., Onishi, H., Takahashi, K., and Watanabe, S. (1978) J. Biochem. (Tokyo) 84, 1529-1542). The conformation of the dephosphorylated 10 S monomer is highly unusual in that the 1500 A long myosin tail is folded into approximately equal thirds (Onishi, H., and Wakabayashi, T. (1982) J. Biochem. (Tokyo) 92, 871-879; Trybus, K. M., Huiatt, T. W., and Lowey, S. (1982) Proc. Natl. Acad. Sci. U. S. A. 79, 6151-6155). It was recently reported that phosphorylation of the regulatory light chain causes the bent monomer to unfold to the extended conformation characteristic of 6 S myosin in high salt (Craig, R., Smith, R., and Kendrick-Jones, J. (1983) Nature (Lond.) 302, 436-439). Here we show that phosphorylated myosin can exist in a stable 10 S conformation provided that the salt concentration is kept sufficiently low. Only in a narrow range of salt concentration does the monomer conformation depend on the state of phosphorylation. Above 0.3 M KCl, all myosins revert to the extended form; below 0.1 M KCl, all monomeric myosin is folded. As the salt concentration is decreased to 0.05 M KCl, the 10 S monomers form antiparallel folded dimers. Because phosphorylation increases filament formation even when 10 S monomer remains in equilibrium with polymer, assembly could proceed via the association of 10 S monomers or by a transient 6 S intermediate.

• Publication year

  1984

• Venue

  Journal of Biological Chemistry

• Publication date

  1984-07-10

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(17)39767-3PMID 6610679
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Correlation of enzymatic properties and conformation of smooth muscle myosin.

  1983cited by this paper
• Light-chain phosphorylation controls the conformation of vertebrate non-muscle and smooth muscle myosin molecules

  1983cited by this paper
• Electron microscopic studies of myosin molecules from chicken gizzard muscle II: The effect of thiophosphorylation of the 20K-dalton light chain on the ATP-induced change in the conformation of myosin monomers.

  1983cited by this paper
• Fibronectin in extended and compact conformations. Electron microscopy and sedimentation analysis.

  1983cited by this paper
• Dependence of the shape of the plasma fibronectin molecule on solvent composition. Ionic strength and glycerol content.

  1983cited by this paper
• Transmission of conformational change in insulin

  1983cited by this paper
• Electron microscopic studies of myosin molecules from chicken gizzard muscle I: the formation of the intramolecular loop in the myosin tail.

  1982cited by this paper
• A bent monomeric conformation of myosin from smooth muscle.

  1982cited by this paper
• Regulation of myosin-filament assembly by light-chain phosphorylation.

  1982cited by this paper
• Adenosine triphosphate-induced reversible change in the conformation of chicken gizzard myosin and heavy meromyosin.

  1982cited by this paper
• Three-dimensional reconstruction of thin filaments decorated with a Ca2+-regulated myosin.

  1982cited by this paper
• Purification and characterization of smooth muscle myosin light chain kinase.

  1981cited by this paper
• Polymerization of myosin from smooth muscle of the calf aorta.

  1981cited by this paper
• Myosin filaments have non-phosphorylated light chains in relaxed smooth muscle

  1981cited by this paper
• Reversible phosphorylation of smooth muscle myosin, heavy meromyosin, and platelet myosin.

  1981cited by this paper
• Myosin phosphorylation and the cross-bridge cycle in arterial smooth muscle.

  1981cited by this paper
• Rotary shadowing of extended molecules dried from glycerol.

  1980cited by this paper
• Structure and function of chicken gizzard myosin.

  1978cited by this paper
• Shape and flexibility of the myosin molecule.

  1978cited by this paper
• Mode of filament assembly of myosins from muscle and nonmuscle cells.

  1978cited by this paper
• Assembly of smooth muscle myosin into side-polar filaments

  1977cited by this paper
• Effect of adenosine di- and triphosphates on the stability of synthetic myosin filaments.

  1972cited by this paper
• An electrophoretic study of the low-molecular-weight components of myosin.

  1970cited by this paper
• ULTRASTRUCTURAL STUDIES ON THE CONTRACTILE MECHANISM OF SMOOTH MUSCLE

  1969cited by this paper
• Substructure of the myosin molecule as visualized by electron microscopy.

  1967cited by this paper

• "QuickStainer": a rapid negative staining device for improved preservation of molecular structure.

  2026cites this paper
• “QuickStainer”: a rapid negative staining device for improved preservation of molecular structure

  2025cites this paper
• Reassessing the unifying hypothesis for hypercontractility caused by myosin mutations in hypertrophic cardiomyopathy

  2024cites this paper
• Non-Muscle Myosin II A: Friend or Foe in Cancer?

  2024cites this paper
• Engines of change: Nonmuscle myosin II in mechanobiology.

  2024cites this paper
• Divergence of cytokinesis and dimorphism control by myosin II regulatory light chain in fission yeasts

  2023cites this paper
• Visualization of cardiac thick filament dynamics in ex vivo heart preparations.

  2023cites this paper
• UNC‐82/NUAK kinase is required by myosin A, but not myosin B, to assemble and function in the thick filament arms of C. elegans striated muscle

  2023cites this paper
• Membrane Adhesion Junctions Regulate Airway Smooth Muscle Function and Phenotype.

  2023cites this paper
• Myosin II regulatory light chain phosphorylation and formin availability modulate cytokinesis upon changes in carbohydrate metabolism

  2022cites this paper
• II folds into a 10S form via two of tail for dynamic subcellular localization

  2022influential citation
• Structural changes induced in Ca2+-regulated myosin filaments Structural changes induced in Ca2+-regulated myosin filaments by Ca2+ and ATP by Ca2+ and ATP

  2022cites this paper
• Dilated cardiomyopathy mutation E525K in human beta-cardiac myosin stabilizes the interacting-heads motif and super-relaxed state of myosin

  2022cites this paper
• Review on the structural understanding of the 10S myosin II in the era of Cryo-electron microscopy

  2022influential citation
• Filament evanescence of myosin II and smooth muscle function

  2021cites this paper
• Targeting cytoskeletal phosphorylation in cancer

  2021influential citation
• Muscle myosins form folded monomers, dimers, and tetramers during filament polymerization in vitro

  2020influential citation
• Linking the Landscape of MYH9-Related Diseases to the Molecular Mechanisms that Control Non-Muscle Myosin II-A Function in Cells

  2020cites this paper
• Cryo-EM structure of the inhibited (10S) form of myosin II

  2020cites this paper
• The central role of the tail in switching off 10S myosin II activity

  2019cites this paper
• The mesa trail and the interacting heads motif of myosin II.

  2019cites this paper
• Evaluation of nanoparticle tracking analysis for the detection of rod-shaped particles and protein aggregates.

  2019cites this paper
• The Central Role of the Tail in Switching Off Myosin II in Cells

  2019cites this paper
• Deciphering the super relaxed state of human β-cardiac myosin and the mode of action of mavacamten from myosin molecules to muscle fibers

  2018cites this paper
• Interacting-heads motif has been conserved as a mechanism of myosin II inhibition since before the origin of animals

  2018cites this paper
• Polymerization pathway of mammalian nonmuscle myosin 2s

  2018influential citation
• Mutation in the tail region of MYH9 inhibits disassembly of nonmuscle myosin IIA

  2017cites this paper
• Non‐muscle (NM) myosin heavy chain phosphorylation regulates the formation of NM myosin filaments, adhesome assembly and smooth muscle contraction

  2017influential citation
• Genome Analysis and Human Health

  2017cites this paper
• Myo2 Motor Function in the Contractile Ring and the Regulation of Fission Yeast Cytokinesis

  2017cites this paper
• Fission yeast myosin Myo2 is down-regulated in actin affinity by light chain phosphorylation

  2017cites this paper
• Effect of ATP and regulatory light-chain phosphorylation on the polymerization of mammalian nonmuscle myosin II

  2017cites this paper
• Non-muscle myosin II motor proteins in human health and diseases

  2017cites this paper
• Positively charged residues within the MYO19 MyMOMA domain are essential for proper localization of MYO19 to the mitochondrial outer membrane

  2016cites this paper
• Peripheral Airway Smooth Muscle, but Not the Trachealis, Is Hypercontractile in an Equine Model of Asthma.

  2016cites this paper
• Myosins: Domain Organisation, Motor Properties, Physiological Roles and Cellular Functions.

  2016cites this paper
• Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.

  2015cites this paper
• to structural malleability Emergence of airway smooth muscle functions related

  2015cites this paper
• Investigating Myosin Light Chain Kinase Binding with Actin in Human Airway Smooth Muscle Cells

  2015cites this paper
• Diffusion of myosin light chain kinase on actin: A mechanism to enhance myosin phosphorylation rates in smooth muscle

  2015cites this paper
• The role of caldesmon and its phosphorylation by ERK on the binding force of unphosphorylated myosin to actin.

  2014cites this paper
• Life without double-headed non-muscle myosin II motor proteins

  2014cites this paper
• Forces measured with micro-fabricated cantilevers during actomyosin interactions produced by filaments containing different myosin isoforms and loop 1 structures.

  2013cites this paper
• Forces measured with micro-fabricated cantilevers during actomyosin interactions produced by filaments containing different myosin isoforms and loop 1 structures.

  2013cites this paper
• Regulatory elements of Drosophila non-muscle myosin II

  2013influential citation
• leukemogenic inversion 16 chimera Molecular basis for a dominant inactivation of RUNX1/AML1 by the

  2013cites this paper
• Characterization of Three Full-length Human Nonmuscle Myosin II Paralogs*

  2013cites this paper
• The heavy chain has its day

  2013cites this paper
• Avian synaptopodin 2 (fesselin) stabilizes myosin filaments and actomyosin in the presence of ATP.

  2013cites this paper
• Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization

  2013cites this paper
• Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.

  2013cites this paper
• Contraction of Stress Fibers Extracted from Smooth Muscle Cells: Effects of Varying Ionic Strength

  2012cites this paper
• 4.8 Myosin Motors: Structural Aspects and Functionality

  2012cites this paper
• 4.14 Smooth Muscle and Myosin Regulation

  2012cites this paper
• Phosphorylated smooth muscle heavy meromyosin shows an open conformation linked to activation.

  2012cites this paper
• MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility.

  2012cites this paper
• Role of the tail in the regulated state of myosin 2.

  2011cites this paper
• Direct evidence for functional smooth muscle myosin II in the 10S self-inhibited monomeric conformation in airway smooth muscle cells

  2011cites this paper
• Emergence of airway smooth muscle functions related to structural malleability.

  2011cites this paper
• Inhibition of MLC Phosphorylation Restricts Replication of Influenza Virus—A Mechanism of Action for Anti-Influenza Agents

  2011cites this paper
• Regulatory light chain phosphorylation and N-terminal extension increase cross-bridge binding and power output in Drosophila at in vivo myofilament lattice spacing.

  2011cites this paper
• Association between α4 integrin cytoplasmic tail and non-muscle myosin IIA regulates cell migration

  2011cites this paper
• Reconstitution of contractile actomyosin bundles.

  2011cites this paper
• Common Structural Motifs for the Regulation of Divergent Class II Myosins*

  2010cites this paper
• Multiple tail domain interactions stabilize nonmuscle myosin II bipolar filaments

  2010cites this paper
• Modeling smooth muscle myosin's two heads: long-lived enzymatic roles and phosphorylation-dependent equilibria.

  2010cites this paper
• Mechanical and structural plasticity.

  2010cites this paper
• Cell Biological and Microfabrication Approaches Towards the Understanding of Transmigration and Nonmuscle Myosin II Assembly

  2010influential citation
• Chronic activation in shortened airway smooth muscle: a synergistic combination underlying airway hyperresponsiveness?

  2010cites this paper
• Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cells.

  2010cites this paper
• Drosophila non-muscle myosin II bipolar filament formation: Importance of charged residues and specific domains for self-assembly

  2009influential citation
• A FERM domain autoregulates Drosophila myosin 7a activity

  2009cites this paper
• Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.

  2009cites this paper
• Phosphorylation and the N-terminal extension of the regulatory light chain help orient and align the myosin heads in Drosophila flight muscle.

  2009cites this paper
• Disrupting actin-myosin-actin connectivity in airway smooth muscle as a treatment for asthma?

  2009cites this paper
• Smooth Muscle Heavy Meromyosin Phosphorylated on One of Its Two Heads Supports Force and Motion*

  2009cites this paper
• Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes.

  2009cites this paper
• Regulation of the function of mammalian myosin and its conformational change.

  2008cites this paper
• Folding and regulation in myosins II and V

  2008cites this paper
• Affinity for MgADP and force of unbinding from actin of myosin purified from tonic and phasic smooth muscle.

  2008influential citation
• Caldesmon and thin-filament regulation of muscle contraction

  2008cites this paper
• Identification and Characterization of Myosin from Rat Testicular Peritubular Myoid Cells1

  2008cites this paper
• Smooth-Muscle Myosin II

  2008cites this paper
• Non-Muscle Myosin II

  2008cites this paper
• Head-head and head-tail interaction: a general mechanism for switching off myosin II activity in cells.

  2008cites this paper
• Myosin V from head to tail

  2008cites this paper
• Physical Integrity of Smooth Muscle Myosin Filaments is Enhanced by Phosphorylation of the Regulatory Myosin Light Chain

  2007cites this paper
• The N-terminal Lobes of Both Regulatory Light Chains Interact with the Tail Domain in the 10 S-inhibited Conformation of Smooth Muscle Myosin*

  2006cites this paper
• Myosin filament assembly in an ever-changing myofilament lattice of smooth muscle.

  2005cites this paper
• Conformational change and regulation of myosin molecules.

  2005cites this paper
• Quantitative atomic force microscopy image analysis of unusual filaments formed by the Acanthamoeba castellanii myosin II rod domain.

  2005cites this paper
• Critical regions for assembly of vertebrate nonmuscle myosin II.

  2005cites this paper
• Site-directed spin labeling reveals a conformational switch in the phosphorylation domain of smooth muscle myosin.

  2005cites this paper
• Molecular basis for a dominant inactivation of RUNX1/AML1 by the leukemogenic inversion 16 chimera.

  2004cites this paper
• Cryo-atomic Force Microscopy of Unphosphorylated and Thiophosphorylated Single Smooth Muscle Myosin Molecules*

  2003cites this paper
• Essential light chain modulates phosphorylation-dependent regulation of smooth muscle myosin.

  2002cites this paper
• Myosin light chain phosphorylation facilitates in vivo myosin filament reassembly after mechanical perturbation.

  2002cites this paper
• The Tip of the Coiled-coil Rod Determines the Filament Formation of Smooth Muscle and Nonmuscle Myosin*

  2001cites this paper
• Myosin assembly critical for the enzyme activity of smooth muscle myosin phosphatase: effects of MgATP, ionic strength, and Mg(2+).

  2001cites this paper
• Macrophage caldesmon is an actin bundling protein.

  2001cites this paper