Purification and characterization of transcription factor IIIC2.

Transcription factor IIIC2 (TFIIIC2), together with other transcription factors (TFIIIB and TFIIIC1), is required for the in vitro transcription of tRNA and adenovirus VA genes by RNA polymerase III. Previous studies have shown that TFIIIC2 is a high molecular weight (approximately 500,000) protein which binds with high affinity to the B-box promoter element of tRNA-type genes. A polypeptide of Mr approximately 250,000 is in close association with DNA in the specific complex between TFIIIC2 and the B-box promoter element. Here we describe the purification of TFIIIC2 by a factor of approximately 25,000 from nuclear extracts of HeLa cells by ionic exchange, affinity, and hydrophobic chromatography and sedimentation velocity centrifugation. The most purified fractions contain polypeptides of approximately 230 kDa (corresponding to the polypeptide which can be cross-linked to VA1 DNA), 110, 100, 80, and 60 kDa which co-sediment with TFIIIC2 B-box specific binding and in vitro transcriptional activities.

• Publication year

  1989

• Venue

  Journal of Biological Chemistry

• Publication date

  1989-06-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)81682-9PMID 2732244
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transcription by RNA polymerase III.

  1988cited by this paper
• Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID

  1988cited by this paper
• Function of a yeast TATA element-binding protein in a mammalian transcription system

  1988cited by this paper
• Ordering promoter binding of class III transcription factors TFIIIC1 and TFIIIC2

  1988cited by this paper
• Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.

  1988cited by this paper
• Activation of transcription factor IIIC by the adenovirus E1A protein.

  1988cited by this paper
• Multiple proteins bind to VA RNA genes of adenovirus type 2

  1987cited by this paper
• Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: inactivation of specific transcription factors

  1987cited by this paper
• The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.

  1987cited by this paper
• Resolution of human transcription factor TFIIIC into two functional components.

  1987cited by this paper
• Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.

  1987cited by this paper
• DNA-binding properties and characterization of human transcription factor TFIIIC2.

  1987cited by this paper
• Separation of TFIIIC into two functional components by sequence specific DNA affinity chromatography.

  1987cited by this paper
• Rapid enrichment of HeLa transcription factors IIIB and IIIC by using affinity chromatography based on avidin-biotin interactions

  1986cited by this paper
• Affinity purification of sequence-specific DNA binding proteins.

  1986cited by this paper
• Selective proteolysis defines two DNA binding domains in yeast transcription factor τ

  1986cited by this paper
• Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.

  1986cited by this paper
• A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor

  1986cited by this paper
• Adenovirus stimulation of transcription by RNA polymerase III: evidence for an E1A‐dependent increase in transcription factor IIIC concentration.

  1986cited by this paper
• Purification of nuclear factor I by DNA recognition site affinity chromatography.

  1986cited by this paper
• Enhancement of RNA polymerase III transcription by the E1A gene product of adenovirus.

  1985cited by this paper
• A split binding site for transcription factor tau on the tRNA3Glu gene.

  1985cited by this paper
• Transcription of class III genes activated by viral immediate early proteins.

  1985cited by this paper
• Differential activation of RNA polymerase III-transcribed genes by the polyomavirus enhancer and the adenovirus E1A gene products.

  1985cited by this paper
• Repetitive zinc‐binding domains in the protein transcription factor IIIA from Xenopus oocytes.

  1985cited by this paper
• Effects of temperature and single-stranded DNA on the interaction of an RNA polymerase III transcription factor with a tRNA gene.

  1985cited by this paper
• Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.

  1984cited by this paper
• Correlations between transcription of a yeast tRNA gene and transcription factor-DNA interactions.

  1984cited by this paper
• Transcription by RNA polymerase III.

  1983cited by this paper
• Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

  1983cited by this paper
• Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.

  1980cited by this paper
• A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

  1980cited by this paper
• A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase.

  1977cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• Structural insights into human TFIIIC promoter recognition

  2023influential citation
• Regulation of tRNA synthesis by posttranslational modifications of RNA polymerase III subunits.

  2017cites this paper
• Specific and programmable release of target molecules via DNA strand displacement reaction

  2014cites this paper
• Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription

  2014cites this paper
• Transcription termination by the eukaryotic RNA polymerase III.

  2013cites this paper
• Viral subversion of host functions for picornavirus translation and RNA replication.

  2012cites this paper
• Regulation of RNA polymerase III transcription by DNA methylation and chromatin

  2012cites this paper
• RNA polymerase III transcription control elements: themes and variations.

  2012cites this paper
• Heat shock factor binding in Alu repeats expands its involvement in stress through an antisense mechanism

  2011cites this paper
• Novel insights into human RNA polymerase III transcription: non canonical termination and biogenesis of potential regulatory RNAs

  2010cites this paper
• Regulation of mammalian gene expression by retroelements and non‐coding tandem repeats

  2008influential citation
• In vivo studies of repressors of RNA polymerase III transcription

  2008cites this paper
• Different Functional Modes of p300 in Activation of RNA Polymerase III Transcription from Chromatin Templates

  2008cites this paper
• Identification, Molecular Cloning, and Characterization of the Sixth Subunit of Human Transcription Factor TFIIIC*

  2007cites this paper
• A test of the model that RNA polymerase III transcription is regulated by selective induction of the 110 kDa subunit of TFIIIC

  2006cites this paper
• Reconstitution of the Yeast RNA Polymerase III Transcription System with All Recombinant Factors*

  2006cites this paper
• Regulation of RNA polymerase III transcription during differentiation.

  2005cites this paper
• The regulation of Pol III transcription by mTOR.

  2005cites this paper
• A minimal RNA polymerase III transcription system from human cells reveals positive and negative regulatory roles for CK2.

  2003cites this paper
• The interaction of cytoplasmic RNA viruses with the nucleus.

  2003cites this paper
• Recruitment of RNA polymerase III to its target promoters.

  2002influential citation
• Characterization of RNA polymerase III transcription factor TFIIIC from the mulberry silkworm, Bombyx mori.

  2002cites this paper
• Effects of Picornavirus Proteinases on Host Cell Transcription

  2002cites this paper
• Evidence for a posttranscriptional role of a TFIIICalpha-like protein in Chironomus tentans.

  2002cites this paper
• Multiple Mechanisms Contribute to the Activation of RNA Polymerase III Transcription in Cells Transformed by Papovaviruses*

  2002cites this paper
• The RNA polymerase III transcription apparatus.

  2001cites this paper
• Genetic interactions within TFIIIC, the promoter-binding factor of yeast RNA polymerase III

  2001cites this paper
• RNA polymerase III transcription factor TFIIIC2 is overexpressed in ovarian tumors.

  2000cites this paper
• Survey and summary: transcription by RNA polymerases I and III.

  2000cites this paper
• RNA polymerase III transcription: its control by tumor suppressors and its deregulation by transforming agents.

  2000cites this paper
• The Retinoblastoma Tumor Suppressor Protein Targets Distinct General Transcription Factors To Regulate RNA Polymerase III Gene Expression

  2000cites this paper
• Transcription efficiency of human polymerase III genes in vitro does not depend on the RNP-forming autoantigen La.

  2000cites this paper
• Nuclear factor 1 (NF1) affects accurate termination and multiple‐round transcription by human RNA polymerase III

  2000cites this paper
• Cloning and Characterization of Two Evolutionarily Conserved Subunits (TFIIIC102 and TFIIIC63) of Human TFIIIC and Their Involvement in Functional Interactions with TFIIIB and RNA Polymerase III

  1999cites this paper
• Human TFIIIC Relieves Chromatin-Mediated Repression of RNA Polymerase III Transcription and Contains an Intrinsic Histone Acetyltransferase Activity

  1999cites this paper
• Lead inhibition of DNA-binding mechanism of Cys(2)His(2) zinc finger proteins.

  1999cites this paper
• The TFIIIC90 Subunit of TFIIIC Interacts with Multiple Components of the RNA Polymerase III Machinery and Contains a Histone-Specific Acetyltransferase Activity

  1999cites this paper
• Activation of RNA Polymerase III Transcription in Cells Transformed by Simian Virus 40

  1999cites this paper
• Regulation of RNA Polymerase III Transcription

  1998cites this paper
• DNA topoisomerase I and PC4 can interact with human TFIIIC to promote both accurate termination and transcription reinitiation by RNA polymerase III.

  1998cites this paper
• A Chimeric Subunit of Yeast Transcription Factor IIIC Forms a Subcomplex with τ95

  1998influential citation
• Tau91, an essential subunit of yeast transcription factor IIIC, cooperates with tau138 in DNA binding.

  1998cites this paper
• The activity binding to the termination region of several pol III genes represents a separate entity and is distinct from a novel component enhancing U6 snRNA transcription.

  1998cites this paper
• τ91, an Essential Subunit of Yeast Transcription Factor IIIC, Cooperates with τ138 in DNA Binding

  1998influential citation
• Transcription Factors Utilized by RNA Polymerase III

  1998cites this paper
• Human transcription factors IIIC2 , IIIC1 and a novel component IIIC0 fulfil different aspects of DNA binding to various pol III genes.

  1997cites this paper
• Specific binding sites for a pol III transcriptional repressor and pol II transcription factor YY1 within the internucleosomal spacer region in primate Alu repetitive elements.

  1996cites this paper
• RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB

  1996cites this paper
• A human B‐box‐binding protein downregulated in adenovirus 5‐transformed human cells

  1996cites this paper
• RNA polymerase III and class III transcription factors from Saccharomyces cerevisiae.

  1996cites this paper
• DNA binding domain and subunit interactions of transcription factor IIIC revealed by dissection with poliovirus 3C protease

  1996cites this paper
• TFIIIC1 acts through a downstream region to stabilize TFIIIC2 binding to RNA polymerase III promoters

  1996cites this paper
• Transcription of Eukaryotic tRNA Genes

  1995cites this paper
• Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors.

  1995cites this paper
• Transcription of adenovirus RNA polymerase III genes.

  1995cites this paper
• RNA polymerase III dependence of the human L1 promoter and possible participation of the RNA polymerase II factor YY1 in the RNA polymerase III transcription system.

  1995cites this paper
• A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors.

  1994cites this paper
• Induction of Drosophila RNA polymerase III gene expression by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is mediated by transcription factor IIIB

  1994cites this paper
• Purification and characterization of human transcription factor IIIA.

  1994cites this paper
• Calcium-dependent inactivation of RNA polymerase III transcription.

  1994cites this paper
• Isolation of transcription factor IIIC from Dictyostelium discoideum.

  1994cites this paper
• Human transcription factor IIIC box B binding subunit.

  1994cites this paper
• TATA binding protein and associated factors in mammalian transcription : characterization of the B-TFIID activity

  1994cites this paper
• Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC

  1994influential citation
• Probing DNA-protein interactions in vitro with the CpG DNA methyltransferase.

  1993cites this paper
• RNA polymerase III. Genes, factors and transcriptional specificity.

  1993cites this paper
• The tyrosine phosphatase cdc25 selectively inhibits transcription of the Xenopus oocyte-type tRNAtyrC gene.

  1993cites this paper
• Gene expression: surprises from the class III side

  1993cites this paper
• The novel purification of functional RNA polymerase III VAI transcription preinitiation complexes.

  1993cites this paper
• Functional characterization of elements in a human U6 small nuclear RNA gene distal control region

  1993cites this paper
• On the subunit composition, stoichiometry, and phosphorylation of the yeast transcription factor TFIIIC/tau.

  1993cites this paper
• TATA-binding protein and associated factors in polymerase II and polymerase III transcription

  1993cites this paper
• Purification and characterization of two forms of human transcription factor IIIC.

  1992cites this paper
• Differential expression of oocyte-type class III genes with fraction TFIIIC from immature or mature oocytes

  1992cites this paper
• Interaction of Xenopus TFIIIC with the TFIIIA.5 S RNA gene complex.

  1992cites this paper
• A TBP complex essential for transcription from TATA-less but not TATA-containing RNA polymerase III promoters is part of the TFIIIB fraction.

  1992cites this paper
• TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC.

  1992cites this paper
• A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.

  1992cites this paper
• Cloning of TFC1, the Saccharomyces cerevisiae gene encoding the 95-kDa subunit of transcription factor TFIIIC.

  1992cites this paper
• The Xenopus YB3 protein binds the B box element of the class III promoter.

  1991cites this paper
• Displacement of Xenopus transcription factor IIIA from a 5S rRNA gene by a transcribing RNA polymerase

  1991cites this paper
• RNA polymerase III (C) and its transcription factors.

  1991cites this paper
• Displacement ofXenopusTranscription Factor IIIAfroma 5S rRNAGenebya Transcribing RNA Polymerase

  1991cites this paper
• The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.

  1991cites this paper
• Purification of sequence-specific binding proteins by DNA affinity chromatography.

  1991cites this paper
• Sarkosyl defines three intermediate steps in transcription initiation by RNA polymerase III: application to stimulation of transcription by E1A.

  1990cites this paper
• Purification and characterization of Saccharomyces cerevisiae transcription factor TFIIIC. Polypeptide composition defined with polyclonal antibodies.

  1990cites this paper
• The hepatitis B virus X‐gene product trans‐activates both RNA polymerase II and III promoters.

  1990cites this paper
• The identification of two antagonistic activities in a Xenopus oocyte extract that can modulate the in vitro transcription of RNA polymerase III genes.

  1990cites this paper
• Specific interaction of a partially purified Xenopus transcription factor IIIC (TFIIIC) with frog tRNA gene.

  1990cites this paper
• In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein

  1990cites this paper
• A transcriptionally active form of TFIIIC is modified in poliovirus-infected HeLa cells

  1990influential citation
• Human transcription factor IIIC contains a polypeptide of 55 kDa specifically binding to Pol III genes.

  1990cites this paper
• Photoaffinity labeling of RNA polymerase III transcription complexes by nascent RNA.

  1990cites this paper
• Unraveling the complexities of transcription by RNA polymerase III.

  1990cites this paper
• Affinity chromatographic purification of a protein which binds specifically to the yeast leucine tRNA gene.

  1990cites this paper
• InVitro Transcriptional Activation, Dimerization, andDNA-Binding Specificity oftheEpstein-Barr VirusZtaProtein

  1990cites this paper
• A DNA-binding domain of human transcription factor IIIC2.

  1989cites this paper
• Regulation of eukaryotic transcription factors by post-translational modification.

  1989cites this paper
• Identification of a wound-induced inhibitor of a nuclear factor that binds the carrot extensin gene

  1989cites this paper