Making antibody fragments using phage display libraries

No abstract is available for this paper.

• Publication year

  1991

• Venue

  Nature

• Publication date

  1991-08-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/352624A0PMID 1907718
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning.

  1991cited by this paper
• Sequences of proteins of immunological interest

  1991cited by this paper
• Man-made antibodies

  1991cited by this paper
• PCR: a practical approach.

  1991cited by this paper
• Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.

  1991cited by this paper
• Use of 2D NMR, protein engineering, and molecular modeling to study the hapten-binding site of an antibody Fv fragment against 2-phenyloxazolone.

  1991cited by this paper
• Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage lambda immunoexpression library.

  1990cited by this paper
• Random peptide libraries: a source of specific protein binding molecules.

  1990cited by this paper
• Hormone phage: An enrichment method for variant proteins with altered binding properties

  1990cited by this paper
• Regulated expression of foreign genes fused to lac: control by glucose levels in growth medium.

  1990cited by this paper
• A single-step procedure for cloning and selection of antibody-secreting hybridomas.

  1990cited by this paper
• Searching for peptide ligands with an epitope library.

  1990cited by this paper
• Phage antibodies: filamentous phage displaying antibody variable domains

  1990cited by this paper
• Peptides on phage: a vast library of peptides for identifying ligands.

  1990cited by this paper
• Influenza virus hemagglutinin-specific antibodies isolated from a combinatorial expression library are closely related to the immune response of the donor.

  1990cited by this paper
• Three‐dimensional structure determination of an anti‐2‐phenyloxazolone antibody: the role of somatic mutation and heavy/light chain pairing in the maturation of an immune response.

  1990cited by this paper
• A comparison of strategies to stabilize immunoglobulin Fv-fragments.

  1990cited by this paper
• Cloning immunoglobulin variable domains for expression by the polymerase chain reaction.

  1989cited by this paper
• Direct clone characterization from plaques and colonies by the polymerase chain reaction.

  1989cited by this paper
• Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli

  1989cited by this paper
• Immune Network Theory

  1989cited by this paper
• Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

  1989cited by this paper
• Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda.

  1989cited by this paper
• Antibodies: A Laboratory Manual

  1988cited by this paper
• High efficiency transformation of E. coli by high voltage electroporation

  1988cited by this paper
• Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

  1988cited by this paper
• Escherichia coli secretion of an active chimeric antibody fragment.

  1988cited by this paper
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

  1988cited by this paper
• Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli.

  1987cited by this paper
• Production of single-stranded plasmid DNA.

  1987cited by this paper
• Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusion.

  1987cited by this paper
• An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.

  1986cited by this paper
• Molecular events during maturation of the immune response to oxazolone

  1985cited by this paper
• Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.

  1985cited by this paper
• A new classification of mouse VH sequences.

  1984cited by this paper
• Purified lambda regulatory protein cII positively activates promoters for lysogenic development.

  1981cited by this paper
• Purified λ regulatory protein cII positively activates promoters for lysogenic development

  1981cited by this paper
• Transcription antitermination by bacteriophage lambda N gene product.

  1980cited by this paper
• Inheritance of antibody specificity V. Anti-2-phenyloxazolone in the mouse

  1978cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper

• Screening of Anti-Erythropoietin Antibody from Autodisplayed Combinatorial Twin-Chain Fv Libraries

  2026cites this paper
• Contrastive learning for antibody-antigen sequence-to-specificity prediction

  2026cites this paper
• 3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders.

  2026cites this paper
• The Midas touch: Recombinant antibodies transforming diagnostic platforms.

  2026cites this paper
• Technologies for Monoclonal Antibody Discovery and Development

  2025cites this paper
• High-throughput strategies for monoclonal antibody screening: advances and challenges

  2025cites this paper
• Enhancing monoclonal antibody diversity by integrating bulk sorting and machine learning

  2025cites this paper
• Shining a light on VEGF detection: Phage display-mediated immuno-fluorescence assay for precise cancer biomarker analysis

  2025cites this paper
• An IgE antibody targeting HER2 identified by clonal selection restricts breast cancer growth via immune-stimulating activities

  2025cites this paper
• Decoding protein binding plasticity via integrated deep ribosome display and deep learning

  2025cites this paper
• Rotary-Type Atomic Layer Deposition of Aluminum Oxide Coating on Micropowder for Secondary Battery Anode Applications

  2025cites this paper
• Construction and characterization of a novel single-chain variable fragment against Bacillus thuringiensis vegetative insecticidal protein.

  2025cites this paper
• Applying artificial intelligence to accelerate and de-risk antibody discovery

  2024cites this paper
• Translation of Deoxyribonucleic Acid into Synthetic Alpha Helical Peptides for Darwinian Evolution

  2024cites this paper
• Development of a new genotype–phenotype linked antibody screening system

  2024cites this paper
• Two-dimensional high-throughput on-cell screening of immunoglobulins against broad antigen repertoires

  2024cites this paper
• Biolayer interferometry for measuring the kinetics of protein–protein interactions and nanobody binding

  2024cites this paper
• Rapid generation of human recombinant monoclonal antibodies from antibody-secreting cells using ferrofluid-based technology

  2024cites this paper
• Phage display for discovery of anticancer antibodies.

  2024cites this paper
• Meta learning addresses noisy and under-labeled data in machine learning-guided antibody engineering.

  2024cites this paper
• Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits

  2024cites this paper
• Engineering an Escherichia coli strain for production of long single-stranded DNA

  2024cites this paper
• Generation of canine neutralizing antibodies against canine parvovirus by single B cell antibody technology

  2024cites this paper
• Synthetic integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits

  2024cites this paper
• Biosensing-based quality control monitoring of the higher-order structures of therapeutic antibody domains.

  2024cites this paper
• Engineering an Escherichia coli strain for production of long single-stranded DNA

  2024cites this paper
• Generation and Selection of Phage Display Antibody Libraries in Fab Format.

  2024cites this paper
• Navigating directed evolution efficiently: optimizing selection conditions and selection output analysis

  2024cites this paper
• Advancements in the preparation technology of small molecule artificial antigens and their specific antibodies: a comprehensive review.

  2024cites this paper
• A New Architecture for DNA-Templated Synthesis in Which Abasic Sites Protect Reactants from Degradation.

  2024cites this paper
• Monoclonal antibodies: From magic bullet to precision weapon

  2024cites this paper
• Extracellular vesicles for developing targeted hearing loss therapy.

  2024cites this paper
• Meta Learning Improves Robustness and Performance in Machine Learning-Guided Protein Engineering

  2023cites this paper
• Evolution of phage display libraries for therapeutic antibody discovery

  2023cites this paper
• Antibody Isolation from Human Synthetic Libraries of Single-Chain Antibodies and Analysis Using NGS.

  2023cites this paper
• Resistance to host antimicrobial peptides mediates resilience of gut commensals during infection and aging in Drosophila

  2023cites this paper
• Effects of the Magnetic Orientation of M13 Bacteriophage on Phage Display Selection.

  2023cites this paper
• Antibody Libraries as Platforms to Exploring Target and Receptor Pleiotropy

  2023cites this paper
• Construction of Human Immune and Naive scFv Phage Display Libraries.

  2023cites this paper
• A Customizable Suite of Methods to Sequence and Annotate Cattle Antibodies

  2023cites this paper
• Production of nanobodies in Andean camelids and their most common applications: A general review in the medical field.

  2023cites this paper
• Impaired Testicular Function without Altering Testosterone Concentration Using an Anti-Follicular-Stimulating Hormone Receptor (Anti-FSHr) Single-Chain Variable Fragment (scFv) in Long-Tailed Macaques (Macaca fascicularis)

  2023cites this paper
• Construction of Chicken and Ostrich Antibody Libraries.

  2023cites this paper
• Quenchbodies That Enable One-Pot Detection of Antigens: A Structural Perspective

  2023cites this paper
• The power of phages: revolutionizing cancer treatment

  2023cites this paper
• AbNatiV: VQ-VAE-based assessment of antibody and nanobody nativeness for hit selection, humanisation, and engineering

  2023cites this paper
• “Redirecting an anti-IL-1β antibody to bind a new, unrelated and computationally predicted epitope on hIL-17A”

  2023cites this paper
• Methods for Engineering Binders to Multi-Pass Membrane Proteins

  2023cites this paper
• Reducing affinity as a strategy to boost immunomodulatory antibody agonism

  2023cites this paper
• Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display.

  2022cites this paper
• Exploring phage engineering to advance nanobiotechnology

  2022cites this paper
• Higher Affinity Antibodies Bind With Lower Hydration and Flexibility in Large Scale Simulations

  2022cites this paper
• Genome-wide investigation of maize RAD51 binding affinity through phage display

  2022cites this paper
• Selection of Cancer Stem Cell-Targeting Agents Using Bacteriophage Display.

  2022cites this paper
• Protein Engineering: Advances in Phage Display for Basic Science and Medical Research

  2022cites this paper
• Non-specificity as the sticky problem in therapeutic antibody development

  2022cites this paper
• A Novel Specific Single-Chain Variable Fragment Diagnostic System for Viral Hemorrhagic Septicemia Virus

  2022cites this paper
• Next-Generation Molecular Discovery: From Bottom-Up In Vivo and In Vitro Approaches to In Silico Top-Down Approaches for Therapeutics Neogenesis

  2022influential citation
• Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy

  2022cites this paper
• Research Progress and Applications of Multivalent, Multispecific and Modified Nanobodies for Disease Treatment

  2022cites this paper
• Development of therapeutic antibodies for the treatment of diseases

  2022cites this paper
• A dataset comprised of binding interactions for 104,972 antibodies against a SARS-CoV-2 peptide

  2022cites this paper
• DNA-Encoded Libraries: Towards Harnessing their Full Power with Darwinian Evolution.

  2022cites this paper
• Isolation and characterization of human anti-CD20 single-chain variable fragment (scFv) from a Naive human scFv library

  2022cites this paper
• Catalytic Peptides: the Challenge between Simplicity and Functionality

  2022cites this paper
• Identification of a rabbit Ig light chain recombinant protein bound to serum immunoglobulins from different marine fish species.

  2022cites this paper
• Anti-tumor effect of aquaporin 3 monoclonal antibody on syngeneic mouse tumor model

  2022cites this paper
• An alpaca single-domain antibody (VHH) phage-display library constructed by CDR shuffling provided high affinity VHHs against desired protein antigens.

  2022cites this paper
• Antibody display technologies: selecting the cream of the crop

  2021cites this paper
• The history of the antibody as a tool.

  2021cites this paper
• Utilizing Immunocytokines for Cancer Therapy

  2021cites this paper
• Sensing the future of bio-informational engineering

  2021cites this paper
• Discovery of phosphotyrosine-binding oligopeptides with supramolecular target selectivity

  2021cites this paper
• Discovering melamine-specific bioreceptors via phage display, constructing its validation method based on the quenching on nanocomplex, and applying screened bioreceptor to the electrochemical assay of melamine

  2021cites this paper
• A new single-chain variable fragment (scFv) antibody provides sensitive and specific detection of citrus tristeza virus.

  2021cites this paper
• Homing Peptides for Cancer Therapy.

  2021cites this paper
• Recent Advances on the Selection Methods of DNA‐Encoded Libraries

  2021cites this paper
• Bispecific antibody targeting TROP2xCD3 suppresses tumor growth of triple negative breast cancer

  2021cites this paper
• Recent Advances in Cell Surface Display Technologies for Directed Protein Evolution

  2021cites this paper
• Phage display and other peptide display technologies.

  2021cites this paper
• Antibody Phage Display

  2021cites this paper
• Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies

  2021cites this paper
• Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy

  2021cites this paper
• Adeno-Associated Virus (AAV) Gene Delivery: Dissecting Molecular Interactions upon Cell Entry

  2021cites this paper
• A System for the Evolution of Protein-Protein Interaction Inducers.

  2021cites this paper
• The development of a high-affinity conformation-sensitive antibody mimetic using a biocompatible copolymer carrier (iBody)

  2021cites this paper
• Antibody-drug discovery

  2021cites this paper
• Bispecific T Cell Engagers for the Treatment of Multiple Myeloma: Achievements and Challenges

  2021cites this paper
• Phage-Displayed Peptides for Targeting Tyrosine Kinase Membrane Receptors in Cancer Therapy

  2021cites this paper
• Antibody Libraries as Tools to Discover Functional Antibodies and Receptor Pleiotropism

  2021cites this paper
• Production of antibodies in SHuffle Escherichia coli strains.

  2021cites this paper
• Synthesis of libraries and multi-site mutagenesis using a PCR-derived, dU-containing template

  2021cites this paper
• Review: The Nose as a Route for Therapy. Part 2 Immunotherapy

  2021cites this paper
• Targeting Tumors Using Peptides

  2020cites this paper
• Protein Engineering in the Ubiquitin System: Tools for Discovery and Beyond

  2020cites this paper
• Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

  2020cites this paper
• Development of therapeutic antibodies for the treatment of diseases

  2020cites this paper
• Isolation of Polyclonal Single-Chain Fragment Variable (scFv) Antibodies Against Venomous Snakes of Iran and Evaluation of Their Capability in Neutralizing the Venom

  2020cites this paper
• From Polyclonal Sera to Recombinant Antibodies: A Review of Immunological Detection of Gluten in Foodstuff

  2020cites this paper
• Antibody Display Systems

  2020cites this paper