Isolation and functional analysis of histidine-tagged elongation factor Tu.

No abstract is available for this paper.

• Publication year

  1992

• Venue

  European Journal of Biochemistry

• Publication date

  1992-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1111/J.1432-1033.1992.TB17406.XPMID 1446670
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Refined structure of elongation factor EF-Tu from Escherichia coli.

  1992cited by this paper
• Kirromycin‐induced modifications facilitate the separation of EF‐Tu species and reveal intermolecular interactions

  1991cited by this paper
• Kirromycin drastically reduces the affinity of Escherichia coli elongation factor Tu for aminoacyl-tRNA.

  1991cited by this paper
• Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus.

  1991cited by this paper
• Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.

  1991cited by this paper
• Isolation and stability of ternary complexes of elongation factor Tu, GTP and aminoacyl-tRNA.

  1991cited by this paper
• Engineering proteins for purification.

  1990cited by this paper
• Bioassay for trans-activation using purified human immunodeficiency virus tat-encoded protein: trans-activation requires mRNA synthesis.

  1989cited by this paper
• Translational frameshifts induced by mutant species of the polypeptide chain elongation factor Tu of Escherichia coli.

  1989cited by this paper
• Crosslinking of elongation factor Tu to tRNAPhe by trans‐diamminedichloroplatinum (II) Characterization of two crosslinking sites on EF‐Tu

  1989cited by this paper
• Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

  1988cited by this paper
• Structure‐function relationships in the GTP binding domain of EF‐Tu: mutation of Val20, the residue homologous to position 12 in p21.

  1988cited by this paper
• Zone-interference gel electrophoresis: a new method for studying weak protein-nucleic acid complexes under native equilibrium conditions.

  1988cited by this paper
• Effects of the mutation glycine-222----aspartic acid on the functions of elongation factor Tu.

  1987cited by this paper
• New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

  1987cited by this paper
• Rapid and efficient site-specific mutagenesis without phenotypic selection.

  1987cited by this paper
• Transcription of the tRNA-tufB operon of Escherichia coli: activation, termination and antitermination.

  1987cited by this paper
• Structural features in aminoacyl‐tRNAs required for recognition by elongation factor Tu

  1987cited by this paper
• Mutant forms of tufA and tufB independently suppress nonsense mutations.

  1987cited by this paper
• Crosslinking of elongation factor Tu to tRNA(Phe) by trans-diamminedichloroplatinum (II). Characterization of two crosslinking sites in the tRNA.

  1987cited by this paper
• GTPase center of elongation factor Tu is activated by occupation of the second tRNA binding site.

  1986cited by this paper
• Mutants of the elongation factor EF‐Tu, a new class of nonsense suppressors.

  1985cited by this paper
• A second tRNA binding site on elongation factor Tu is induced while the factor is bound to the ribosome.

  1985cited by this paper
• Effect of trypsin modification of the Escherichia coli elongation factor Tu on the ternary complex with aminoacyl-tRNA.

  1985cited by this paper
• Rapid and efficient site-specific mutagenesis without phenotypic selection.

  1985cited by this paper
• Mechanism of action of kirromycin-like antibiotics.

  1985cited by this paper
• Cross-linking of tRNA at two different sites of the elongation factor Tu.

  1984cited by this paper
• Modification of amino groups in EF-Tu.GTP and the ternary complex EF-Tu.GTP.valyl-tRNAVal.

  1984cited by this paper
• Histidine residues in elongation factor EF-tu from Escherichia coli protected by aminoacyl-tRNA against photo-oxidation.

  1984cited by this paper
• The role of EF-Tu in the expression of tufA and tufB genes.

  1983cited by this paper
• N‐tosyl‐L‐phenylalanylchloromethane reacts with cysteine 81 in the molecule of elongation factor Tu from Escherichia coli

  1982cited by this paper
• The antibiotics kirromycin and pulvomycin bind to different sites on the elongation factor Tu from Escherichia coli.

  1982cited by this paper
• Hydrolysis of GTP by elongation factor Tu can be induced by monovalent cations in the absence of other effectors.

  1982cited by this paper
• Identification of a histidine residue near the aminoacyl transfer ribonucleic acid binding site of elongation factor Tu.

  1981cited by this paper
• A simplified procedure for the isolation of bacterial polypeptide elongation factor EF-Tu.

  1980cited by this paper
• Reactivity of amino groups in various complexes of the peptide chain elongation factor EF-Tu from Escherichia coli. A new method of competitive labelling using reductive methylation.

  1978cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper
• Studies on the role of factor Ts in polypeptide synthesis.

  1970cited by this paper

• Optimized purification workflow for advanced eEF1A studies: Impact of His-tagging on stability, functionality, and yield.

  2025cites this paper
• Cotemporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation.

  2022cites this paper
• Production of bioactive recombinant ovine cysteine-rich secretory protein 1 in Escherichia coli

  2021cites this paper
• Broad range of missense error frequencies in cellular proteins

  2019cites this paper
• Co-temporal Force and Fluorescence Measurements Reveal a Ribosomal Gear Shift Mechanism of Translation Regulation by Structured mRNAs.

  2019cites this paper
• IF2 and unique features of initiator tRNAfMet help establish the translational reading frame

  2018cites this paper
• ANKRD16 prevents neuron loss caused by an editing-defective tRNA synthetase

  2018cites this paper
• EF-G catalyzed translocation dynamics in the presence of ribosomal frameshifting stimulatory signals

  2016cites this paper
• The C-terminal Helix of Pseudomonas aeruginosa Elongation Factor Ts Tunes EF-Tu Dynamics to Modulate Nucleotide Exchange*

  2016cites this paper
• Methodology for the analysis of transcription and translation in transcription-coupled-to-translation systems in vitro.

  2015cites this paper
• Kinetic analyses on two translational GTPases : LepA and EF-Tu

  2013cites this paper
• The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu

  2013cites this paper
• Construction of a fully active Cys-less elongation factor Tu: functional role of conserved cysteine 81.

  2011cites this paper
• Properties of Escherichia coli EF-Tu mutants designed for fluorescence resonance energy transfer from tRNA molecules.

  2010cites this paper
• Short spacing between the Shine–Dalgarno sequence and P codon destabilizes codon–anticodon pairing in the P site to promote +1 programmed frameshifting

  2010cites this paper
• Two partners of the ribosome, EF-Tu and LepA

  2009cites this paper
• A biotin-based protein tagging system.

  2009cites this paper
• RNA-dependent lipid remodeling by bacterial multiple peptide resistance factors

  2008cites this paper
• Following translation by single ribosomes one codon at a time

  2008cites this paper
• The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit.

  2008cites this paper
• Intersubunit movement is required for ribosomal translocation

  2007cites this paper
• Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.

  2007cites this paper
• The highly conserved LepA is a ribosomal elongation factor that back-translocates the ribosome.

  2006influential citation
• On the Mechanism of Action of 9-O-Arylalkyloxime Derivatives of 6-O-Mycaminosyltylonolide, a New Class of 16-Membered Macrolide Antibiotics

  2006cites this paper
• Reverse translocation of tRNA in the ribosome.

  2006cites this paper
• Competition between trans-translation and termination or elongation of translation

  2005cites this paper
• The Role of an Activating Peptide in Protease-mediated Suicide of Escherichia coli K12*

  2005cites this paper
• mRNA helicase activity of the ribosome.

  2005cites this paper
• tRNA 3`-CCA ROLLIST TRANSLATSIOONIL JA AMINOHAPPE ROLLIST AMINOATSÜÜL-tRNA SELEKTSIOONIL

  2005cites this paper
• Identification of essential residues within Lit, a cell death peptidase of Escherichia coli K-12.

  2004cites this paper
• Dissecting the ribosomal inhibition mechanisms of edeine and pactamycin: the universally conserved residues G693 and C795 regulate P-site RNA binding.

  2004cites this paper
• Isolation of Qbeta polymerase complexes containing mutant species of elongation factor Tu.

  2003cites this paper
• Isolation of Qβ polymerase complexes containing mutant species of elongation factor Tu

  2003cites this paper
• Mechanism of Elongation Factor (EF)-Ts-catalyzed Nucleotide Exchange in EF-Tu

  2002cites this paper
• Dissection of the mechanism for the stringent factor RelA.

  2002cites this paper
• Immobilized metal-ion affinity chromatography: From phenomenological hallmarks to structure-based molecular insights

  2002cites this paper
• Heterologous expression of maize chloroplast protein synthesis elongation factor (EF-Tu) enhances Escherichia coli viability under heat stress

  2002cites this paper
• Functional Importance of the 3′-Terminal Adenosine of tRNA in Ribosomal Translation*

  2002cites this paper
• Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA.

  2001cites this paper
• Simultaneous and Functional Binding of SmpB and EF-TuGT Pt oth eAlany lAccepto rAr mo ftmRNA

  2001cites this paper
• Efficient separation of Thermus aquaticus EF-Tu functional complexes.

  2001cites this paper
• Complex of Transfer-messenger RNA and Elongation Factor Tu

  2001cites this paper
• Elongation factor G-induced structural change in helix 34 of 16S rRNA related to translocation on the ribosome.

  2001cites this paper
• The variant tuf3 gene of Streptomyces coelicolor A3(2) encodes a real elongation factor Tu, as shown in a novel Streptomyces in vitro translation system.

  2001cites this paper
• GE2270A-resistant mutations in elongation factor Tu allow productive aminoacyl-tRNA binding to EF-Tu.GTP.GE2270A complexes.

  2000cites this paper
• Transit of tRNA through the Escherichia coli ribosome. Cross-linking of the 3' end of tRNA to specific nucleotides of the 23 S ribosomal RNA at the A, P, and E sites.

  2000cites this paper
• Kinetic parameters for tmRNA binding to alanyl-tRNA synthetase and elongation factor Tu from Escherichia coli.

  2000cites this paper
• The Major Head Protein of Bacteriophage T4 Binds Specifically to Elongation Factor Tu*

  2000cites this paper
• The effect of mutations in EF-Tu on its affinity for tRNA as measured by two novel and independent methods of general applicability.

  2000cites this paper
• Point Mutants of Elongation Factor Tu from E. Coli Impaired in Binding Aminoacyl-tRNA

  1999cites this paper
• Either of the chromosomal tuf genes of E. coli K-12 can be deleted without loss of cell viability

  1999cites this paper
• Mutant EF-Tu species reveal novel features of the enacyloxin IIa inhibition mechanism on the ribosome.

  1999cites this paper
• Binding Interaction between Tet(M) and the Ribosome: Requirements for Binding

  1998cites this paper
• A kirromycin-resistant EF-Tu species reverses streptomycin dependence of Escherichia coli strains mutated in ribosomal protein S12.

  1998cites this paper
• Function in vivo of separate segments of the β subunit of Escherichia coli RNA polymerase

  1998cites this paper
• Purification of elongation factors EF-Tu and EF-G from Escherichia coli by covalent chromatography on thiol-sepharose.

  1998cites this paper
• Specific peptide-activated proteolytic cleavage of Escherichia coli elongation factor Tu.

  1998cites this paper
• Natural kirromycin resistance of elongation factor Tu from the kirrothricin producer Streptomyces cinnamoneus.

  1997cites this paper
• The G222D mutation in elongation factor Tu inhibits the codon‐induced conformational changes leading to GTPase activation on the ribosome.

  1996cites this paper
• Translational activities of EF-Tu [G222D] which cannot be reconciled with the classical scheme of the polypeptide chain elongation cycle.

  1996cites this paper
• Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli.

  1995cites this paper
• Substitution of Arg230 and Arg233 in Escherichia coli elongation factor Tu strongly enhances its pulvomycin resistance.

  1995cites this paper
• Complete purification of tRNA, charged or modified with hydrophobic groups, by reversed-phase high-performance liquid chromatography on a C4/C18 column system

  1994cites this paper
• Elongation Factor Tu, a Model Protein for Studying Structural-Functional Relationships and Regulatory Mechanisms

  1994cites this paper
• Elongation factors in protein synthesis.

  1993cites this paper
• Specific Functions of Elongation Factor Tu, a Molecular Switch in Protein Biosynthesis, as Studied by Site-Directed Mutagenesis

  1993cites this paper
• Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins

  1993cites this paper