Point Mutants of Elongation Factor Tu from E. Coli Impaired in Binding Aminoacyl-tRNA

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• Publication year

  1999

• Venue

  Unknown venue

• Publication date

  Unknown publication date

• Fields of study

  Biology, Chemistry

• Identifiers
  DOI 10.1007/978-94-011-4485-8_14
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• WHOLE, UNMODIFIED, EF-TU(ELONGATION FACTOR TU).

  1999cited by this paper
• Investigation of Functional Aspects of the N-terminal Region of Elongation Factor Tu from Escherichia coli Using a Protein Engineering Approach*

  1998cited by this paper
• Solution structure of the ternary complex between aminoacyl-tRNA, elongation factor Tu, and guanosine triphosphate.

  1998cited by this paper
• The role of Glu259 in Escherichia coli elongation factor Tu in ternary complex formation.

  1998cited by this paper
• Molecular movement inside the translational engine.

  1998cited by this paper
• Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors

  1998cited by this paper
• Visualization of elongation factor G on the Escherichia coli 70S ribosome: the mechanism of translocation.

  1998cited by this paper
• The 80S rat liver ribosome at 25 A resolution by electron cryomicroscopy and angular reconstitution.

  1998cited by this paper
• The stretch of C-terminal acidic amino acids of translational release factor eRF1 is a primary binding site for eRF3 of fission yeast.

  1998cited by this paper
• Protein biosynthesis: structural studies of the elongation cycle

  1998cited by this paper
• Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing.

  1998cited by this paper
• Mutational analysis of Glu272 in elongation factor 1A of E. coli

  1998cited by this paper
• An intact conformation at the tip of elongation factor G domain IV is functionally important

  1998cited by this paper
• Escherichia coli 70 S ribosome at 15 A resolution by cryo-electron microscopy: localization of fMet-tRNAfMet and fitting of L1 protein.

  1998cited by this paper
• Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome

  1997cited by this paper
• Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins.

  1997cited by this paper
• Crystal structure of the EF-Tu˙EF-Ts complex from Thermus thermophilus

  1997cited by this paper
• Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region.

  1997cited by this paper
• Arrangement of tRNAs in pre- and posttranslocational ribosomes revealed by electron cryomicroscopy.

  1997cited by this paper
• Contribution of Arg288 of Escherichia coli elongation factor Tu to translational functionality.

  1997cited by this paper
• The ternary complex of aminoacylated tRNA and EF-Tu-GTP. Recognition of a bond and a fold.

  1996cited by this paper
• Mapping Escherichia coli Elongation Factor Tu Residues Involved in Binding of Aminoacyl-tRNA*

  1996cited by this paper
• The structure of the Escherichia coli EF-Tu· EF-Ts complex at 2.5 Å resolution

  1996cited by this paper
• An α to β conformational switch in EF-Tu

  1996cited by this paper
• The GTP binding motif: variations on a theme

  1996cited by this paper
• Initial Binding of the Elongation Factor Tu·GTP·Aminoacyl-tRNA Complex Preceding Codon Recognition on the Ribosome (*)

  1996cited by this paper
• Protein synthesis: Imprinting through molecular mimicry

  1996cited by this paper
• Direct Visualization of A-, P-, and E-Site Transfer RNAs in the Escherichia coli Ribosome

  1996cited by this paper
• Mapping the rRNA neighborhood of the acceptor end of tRNA in the ribosome.

  1996cited by this paper
• Elongation in bacterial protein biosynthesis.

  1996cited by this paper
• Helix unwinding in the effector region of elongation factor EF-Tu-GDP.

  1996cited by this paper
• Conserved motifs in prokaryotic and eukaryotic polypeptide release factors: tRNA-protein mimicry hypothesis.

  1996cited by this paper
• Mutants of EF‐Tu defective in binding aminoacyl‐tRNA

  1996cited by this paper
• Structure of the ternary complex of EF-Tu: macromolecular mimicry in translation.

  1996cited by this paper
• Mutation of the conserved Gly83 and Gly94 in Escherichia coli elongation factor Tu. Indication of structural pivots.

  1995cited by this paper
• Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding.

  1995cited by this paper
• Structure-based sequence alignment of elongation factors Tu and G with related GTPases involved in translation.

  1995cited by this paper
• The 70S Escherichia coli ribosome at 23 A resolution: fitting the ribosomal RNA.

  1995cited by this paper
• Site‐directed mutagenesis of Thermus thermophilus EF‐Tu: the substitution of threonine‐62 by serine or alanine

  1995cited by this paper
• Selection of an RNA molecule that mimics a major autoantigenic epitope of human insulin receptor.

  1995cited by this paper
• Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase–uracil glycosylase inhibitor protein complex

  1995cited by this paper
• Phosphorylation of Elongation Factor Tu Prevents Ternary Complex Formation (*)

  1995cited by this paper
• Crystal Structure of the Ternary Complex of Phe-tRNAPhe, EF-Tu, and a GTP Analog

  1995cited by this paper
• Antibiotic resistance mechanisms of mutant EF-Tu species in Escherichia coli.

  1995cited by this paper
• Relevance of histidine‐84 in the elongation factor Tu GTPase activity and in poly(Phe) synthesis: Its substitution by glutamine and alanine

  1995cited by this paper
• Mutation of the conserved Gly94 and Gly126 in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles.

  1995cited by this paper
• Three‐dimensional structure of the ribosomal translocase: elongation factor G from Thermus thermophilus.

  1994cited by this paper
• The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution.

  1994cited by this paper
• Histidine‐118 of elongation factor Tu: its role in aminoacyl‐tRNA binding and regulation of the GTPase activity

  1994cited by this paper
• Elongation factor 2 mutants deficient in diphthamide formation show temperature-sensitive cell growth.

  1994cited by this paper
• The structural and functional basis for the kirromycin resistance of mutant EF‐Tu species in Escherichia coli.

  1994cited by this paper
• Escherichia coli elongation-factor-Tu mutants with decreased affinity for aminoacyl-tRNA.

  1994cited by this paper
• Mutations to kirromycin resistance occur in the interface of domains I and III of EF‐Tu·GTP

  1994cited by this paper
• Effector region of the translation elongation factor EF-Tu.GTP complex stabilizes an orthoester acid intermediate structure of aminoacyl-tRNA in a ternary complex.

  1994cited by this paper
• The 530 loop of 16S rRNA: a signal to EF-Tu?

  1994cited by this paper
• Elongation factor Tu: a regulatory GTPase with an integrated effector.

  1994cited by this paper
• A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome

  1993cited by this paper
• Crystal structure of active elongation factor Tu reveals major domain rearrangements

  1993cited by this paper
• Yeast tRNAAsp recognition by its cognate class II aminoacyl-tRNA synthetase

  1993cited by this paper
• The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation.

  1993cited by this paper
• One-step purification of E. coli elongation factor Tu.

  1992cited by this paper
• Refined structure of elongation factor EF-Tu from Escherichia coli.

  1992cited by this paper
• Isolation and functional analysis of histidine-tagged elongation factor Tu.

  1992cited by this paper
• Software Engineering in Health Informatics

  1991cited by this paper
• The GTPase superfamily: conserved structure and molecular mechanism

  1991cited by this paper
• Structure-function relationships of elongation factor Tu as studied by mutagenesis.

  1991cited by this paper
• Suggestions for "safe" residue substitutions in site-directed mutagenesis.

  1991cited by this paper
• Mutagenesis of the NH2-terminal domain of elongation factor Tu.

  1990cited by this paper
• Deciphering the message in protein sequences: tolerance to amino acid substitutions.

  1990cited by this paper
• Interaction of elongation factor Tu from Escherichia coli with aminoacyl-tRNA carrying a fluorescent reporter group on the 3' terminus.

  1989cited by this paper
• Structure-function relationships of elongation factor Tu. Isolation and activity of the guanine-nucleotide-binding domain.

  1989cited by this paper
• Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA

  1988cited by this paper
• Zone-interference gel electrophoresis: a new method for studying weak protein-nucleic acid complexes under native equilibrium conditions.

  1988cited by this paper
• A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.

  1987cited by this paper
• Evidence that the G2661 region of 23S rRNA is located at the ribosomal binding sites of both elongation factors.

  1987cited by this paper
• Properties of a genetically engineered G domain of elongation factor Tu.

  1987cited by this paper
• Structural features in aminoacyl‐tRNAs required for recognition by elongation factor Tu

  1987cited by this paper
• Tinkering with enzymes: what are we learning?

  1987cited by this paper
• Elongation factor Tu localized on the exterior surface of the small ribosomal subunit.

  1986cited by this paper
• Characterization of the elongation factors from calf brain. 2. Functional properties of EF-1 alpha, the action of physiological ligands and kirromycin.

  1986cited by this paper
• Localization of elongation factor Tu on the ribosome

  1986cited by this paper
• Characterization of the elongation factors from calf brain. 3. Properties of the GTPase activity of EF-1 alpha and mode of action of kirromycin.

  1986cited by this paper
• Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X‐ray crystallography.

  1985cited by this paper
• Direct determination of the association constant between elongation factor Tu X GTP and aminoacyl-tRNA using fluorescence.

  1985cited by this paper
• Mechanism of action of kirromycin-like antibiotics.

  1985cited by this paper
• Kinetic studies of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate-aminoacyl-tRNA complexes.

  1985influential reference
• The elongation factor Tu binds aminoacyl-tRNA in the presence of GDP.

  1982cited by this paper
• Localization of the elongation factor g on escherichia coli ribosome

  1981cited by this paper
• Role of the 5'-terminal phosphate of tRNA for its function during protein biosynthesis elongation cycle.

  1980cited by this paper
• The determination of binding parameters from protection experiments. A quantitative assay for ternary complex formation of elongation factor Tu, GTP, and aminoacyl-tRNA.

  1979cited by this paper
• Cross-linking of initiation factor IF2 to proteins L7/L12 in 70 S ribosomes of Escherichia coli.

  1976cited by this paper
• Structure of yeast phenylalanine tRNA at 3 Å resolution

  1974cited by this paper

• Challenges of site-specific selenocysteine incorporation into proteins by Escherichia coli

  2018cites this paper