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A study of mixed cell interactions between sensitized and allogeneic mouse spleen cells in vitro using the hemolytic plaque assay.

S. Hirano,E. Uyeki

Published 1971 in Journal of Immunology

ABSTRACT

By using the in vitro production of PFC (plaque-forming cells) as an indicator of cytotoxic potency, allogeneic spleen cell interactions were investigated. Dispersed spleen cells from two allogeneic mouse strains were mixed at the beginning of the culture period, and a wide range of interaction was observed (i.e., suppression or stimulation of PFC production). The degree of suppression of PFC was generally related to the disparity of the H-2 allele. In some combinations of normal allogeneic lymphoid cells, moderate stimulation of PFC production was observed and was associated with allogeneic strains of weak histoincompatibility. Suppression of PFC production in mixed cultures by effector cells (spleen cells from pre-sensitized animals) had the following characteristics: a) marked suppression of PFC production was observed only when effector cells were combined with specific donor cells, b) initial cell-cell contact was necessary and c) the factor(s) responsible for the suppression of PFC production did not depend on the action of complement and was not detectable in aqueous extracts from effector cells. This factor(s) appeared earlier than humoral antibodies in mice sensitized against target lymphoid cells.

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