High-efficiency transformation of yeast by electroporation.

No abstract is available for this paper.

• Publication year

  1991

• Venue

  Methods in Enzymology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0076-6879(91)94015-5PMID 2005786
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Essential role for phosphatidylinositol 4,5-bisphosphate in yeast cell proliferation

  1988cited by this paper
• Electroporation: High frequency of occurrence of a transient high‐permeability state in erythrocytes and intact yeast

  1988cited by this paper
• High efficiency transformation of E. coli by high voltage electroporation

  1988cited by this paper
• Transformation of Saccharomyces cerevisiae spheroplasts by high electric pulse

  1985cited by this paper
• Transformation of intact yeast cells treated with alkali cations.

  1984cited by this paper

• Engineering of 2-ketoacid Decarboxylases for Production of Isobutanol and Other Fusel Alcohols in Saccharomyces cerevisiae.

  2026cites this paper
• Valproate independently activates Snf1, inhibits TORC1, and induces repression of INO1 transcription by increasing nuclear localization of Opi1

  2025cites this paper
• SHIP identifies genomic safe harbors in eukaryotic organisms using genomic general feature annotation

  2025cites this paper
• Development of ptxD/Phi as a new dominant selection system for genetic manipulation in Cryptococcus neoformans

  2024cites this paper
• Establishment of an optimized electroporation method for Halomonas sp. YK44 and its application in the coproduction of PHB and isobutanol

  2024cites this paper
• A peroxidase-derived ligand that induces Fusarium graminearum Ste2 receptor-dependent chemotropism

  2024cites this paper
• Increasing gene dosage and chaperones co-expression facilitate the efficient dextranase expression in Pichia pastoris

  2023cites this paper
• Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae

  2023cites this paper
• Valproate regulates inositol synthesis by reducing expression of myo-inositol-3-phosphate synthase

  2023cites this paper
• A novel approach to enhance the performance of kallikrein 6 enzyme using Pichia pastoris GS115 as a host

  2023cites this paper
• Biosynthesis of artificial starch and microbial protein from agricultural residue.

  2023cites this paper
• Influence of codon optimization, promoter, and strain selection on the heterologous production of a β-fructofuranosidase from Aspergillus fijiensis ATCC 20611 in Pichia pastoris

  2022cites this paper
• Modulating DNA Repair Pathways to Diversify Genomic Alterations in Saccharomyces cerevisiae

  2022cites this paper
• A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an Industrial Saccharomyces cerevisiae Strain

  2022cites this paper
• Legume NCRs and nodule-specific defensins of actinorhizal plants—Do they share a common origin?

  2022cites this paper
• Heterologous (Over) Expression of Human SoLute Carrier (SLC) in Yeast: A Well-Recognized Tool for Human Transporter Function/Structure Studies

  2022cites this paper
• Combinatorial library design for improving isobutanol production in Saccharomyces cerevisiae

  2022cites this paper
• Engineering Proteins Containing Noncanonical Amino Acids on the Yeast Surface.

  2022cites this paper
• Mapping mitonuclear epistasis using a novel recombinant yeast population

  2022cites this paper
• Factors and Conditions That Impact Electroporation of Clostridioides difficile Strains

  2020cites this paper
• The ploidy determination of the biotechnologically important yeast Candida utilis

  2020cites this paper
• Electrotransformation of Saccharomyces cerevisiae.

  2020cites this paper
• One‐shot optimization of multiple enzyme parameters: Tailoring glucose oxidase for pH and electron mediators

  2020cites this paper
• Characterization and function of a sunflower (Helianthus annuus L.) Class II acyl-CoA-binding protein.

  2020cites this paper
• Cloning of the SPO11 gene that complements a meiotic recombination defect in sake yeast.

  2020cites this paper
• Modified chemical method for efficient transformation and diagnosis in Pichia pastoris.

  2020cites this paper
• Mutagenesis, breeding, and characterization of sake yeast strains with low production of dimethyl trisulfide precursor.

  2020cites this paper
• A High-Throughput Screening System Based on Droplet Microfluidics for Glucose Oxidase Gene Libraries

  2020cites this paper
• Pex14p Phosphorylation Modulates Import of Citrate Synthase 2 Into Peroxisomes in Saccharomyces cerevisiae

  2020cites this paper
• Meiotic chromosomal recombination defect in sake yeasts.

  2019cites this paper
• Species-specific differences in the susceptibility of fungi towards the antifungal protein AFP depend on C3 saturation of glycosylceramides

  2019cites this paper
• A novel method for genetic transformation of C. albicans using modified-hydroxyapatite nanoparticles as a plasmid DNA vehicle

  2019cites this paper
• Species-Specific Differences in the Susceptibility of Fungi to the Antifungal Protein AFP Depend on C-3 Saturation of Glycosylceramides

  2019cites this paper
• Simplified protocol for faster transformation of (a large number of) Pichia pastoris strains

  2019cites this paper
• Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H.

  2019cites this paper
• QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast

  2019cites this paper
• ABCC Transporters Mediate the Vacuolar Accumulation of Crocins in Saffron Stigmas[OPEN]

  2019cites this paper
• Optimization of Aspergillus fijiensis β-fructofuranosidase expression and production using Pichia pastoris, for the production of fructooligosaccharides from sucrose

  2019cites this paper
• A Lysine Mutation of the Claw-Arm-Like Loop Accelerates Catalysis by Cellobiohydrolases.

  2019cites this paper
• An efficient method for isolating mating‐competent cells from bottom‐fermenting yeast using mating pheromone‐supersensitive mutants

  2018cites this paper
• Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp.

  2018cites this paper
• Fusion of plectasin derivative NZ2114 with hydrophilic random coil polypeptide: Recombinant production in Pichia pastoris and antimicrobial activity against clinical strain MRSA

  2018cites this paper
• Expression of Recombinant Genes in the Yeast Pichia pastoris

  2018cites this paper
• Control of the culture conditions of Chlamydomonas reinhardtii for efficient delivery of exogenous materials in electroporation

  2018cites this paper
• Rationally designed perturbation factor drives evolution in Saccharomyces cerevisiae for industrial application

  2018cites this paper
• Tailor-made mannosylerythritol lipids: current state and perspectives

  2018cites this paper
• Genome-Wide Identification and Characterization of Four Gene Families Putatively Involved in Cadmium Uptake, Translocation and Sequestration in Mulberry

  2018cites this paper
• Production and manipulation of blue copper oxidases for technological applications.

  2018cites this paper
• Heterologous Biosynthesis of the Fungal Sesquiterpene Trichodermol in Saccharomyces cerevisiae

  2018cites this paper
• Molecular Characterization of a Geranyl Diphosphate-Specific Prenyltransferase Catalyzing Stilbenoid Prenylation from Morus alba

  2018cites this paper
• Modified protocol for quick and large-scale transformation of Pichia pastoris

  2018cites this paper
• Zinc Finger Proteins

  2018cites this paper
• Gene Editing in Channel Catfish via Double Electroporation of Zinc-Finger Nucleases.

  2018cites this paper
• IDENTIFICATION OF COMPOUNDS CAPABLE OF ABROGATION HIV-1 GAG CYCLOPHILIN COMPLEX FORMATION

  2017cites this paper
• Fast and efficient genetic transformation of oleaginous yeast Rhodosporidium toruloides by using electroporation

  2017cites this paper
• Virus-induced gene silencing of the two squalene synthase isoforms of apple tree (Malus × domestica L.) negatively impacts phytosterol biosynthesis, plastid pigmentation and leaf growth

  2017cites this paper
• β‐Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae

  2017cites this paper
• Impact of Chromosomal Translocations (CTs) on reproductive isolation and fitness in natural yeast isolates

  2017influential citation
• Produção heteróloga de ácido hialurônico em Klyuveromyces lactis

  2017cites this paper
• PEROXISOME ASSEMBLY IN YARROW/A UPOLYTICA PEROXISOME ASSEMBLY IN YARROW/A UPOLYTICA

  2017cites this paper
• Yeast Cytokinesis

  2016cites this paper
• Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast

  2016cites this paper
• Identification of a second PAD1 in Brettanomyces bruxellensis LAMAP2480

  2016cites this paper
• Improved production of fatty acids by Saccharomyces cerevisiae through screening a cDNA library from the oleaginous yeast Yarrowia lipolytica.

  2016cites this paper
• Optimizing Recombinant Adhesion Molecule Constructs for Targeted Therapeutic Nanoparticles

  2016cites this paper
• Nutrient supplements boost yeast transformation efficiency

  2016cites this paper
• Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice.

  2016cites this paper
• A Review of Fluorescent Proteins for Use in Yeast.

  2016cites this paper
• Cloning, expression and cytotoxicity of granulin A, a novel polypeptide contained in human progranulin.

  2016cites this paper
• Structure-Based Design of Mucor pusillus Pepsin for the Improved Ratio of Clotting Activity/Proteolytic Activity in Cheese Manufacture.

  2015cites this paper
• Transformation of the yeast Trichosporonoides oedocephalis

  2015cites this paper
• Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells

  2015cites this paper
• Genetic Manipulation of Meyerozyma guilliermondii

  2015cites this paper
• Electroporation of Pichia pastoris

  2015cites this paper
• Screening and Characterization of a Mutant Fungal Aspartic Proteinase from Mucor pusillus

  2015cites this paper
• A Novel GH7 Endo-β-1,4-Glucanase from Neosartorya fischeri P1 with Good Thermostability, Broad Substrate Specificity and Potential Application in the Brewing Industry

  2015cites this paper
• Transformation of the yeast Trichosporonoides oedocephalis

  2015cites this paper
• Methods for the directed evolution of proteins

  2015cites this paper
• Synthese der Polyketide Flaviolin und Oxytetracyclin in permeabilisierten Zellen genetisch modifizierter Escherichia coli

  2015cites this paper
• The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation

  2014cites this paper
• Torulopsis versatilis strains with increased salt tolerance carry mutations in the glycerol transporter gene FPS1

  2014cites this paper
• The wine and beer yeast Dekkera bruxellensis

  2014cites this paper
• Physical methods for genetic transformation of fungi and yeast.

  2014cites this paper
• Identification of the Dekkera bruxellensis phenolic acid decarboxylase (PAD) gene responsible for wine spoilage

  2014cites this paper
• Phosphorylation of alfalfa mosaic virus movement protein in vivo

  2014cites this paper
• Improvement of Electrotransformation Efficiency by Lysozyme Treatment in Cronobacter sakazakii

  2014cites this paper
• Secretion and assembly of calicivirus-like particles in high-cell-density yeast fermentations: strategies based on a recombinant non-specific BPTI-Kunitz-type protease inhibitor

  2014cites this paper
• Ultra-high-throughput screening method for the directed evolution of glucose oxidase.

  2014cites this paper
• Yeast competence for exogenous DNA uptake: towards understanding its genetic component

  2013cites this paper
• Directed multistep biocatalysis using tailored permeabilized cells.

  2013cites this paper
• A Saccharomyces cerevisiae Assay System to Investigate Ligand/AdipoR1 Interactions That Lead to Cellular Signaling

  2013cites this paper
• A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5.

  2013cites this paper
• Bacteriophage T7 RNA polymerase-based expression in Pichia pastoris.

  2013cites this paper
• Expression of HA1 fragment of H1N1 swine influenza viral protein, hemagglutinin, on the surface of yeast Saccharomyces cerevisiae using pYD5 shuttle vector

  2012cites this paper
• Construction of a new recombinant protein expression system in the basidiomycetous yeast Cryptococcus sp. strain S-2 and enhancement of the production of a cutinase-like enzyme

  2012cites this paper
• Isolation and partial characterisation of PHT1;5, a putative high affinity phosphate transporter from Arabidopsis thaliana

  2012cites this paper
• Blocking Double-Stranded RNA-Activated Protein Kinase PKR by Japanese Encephalitis Virus Nonstructural Protein 2A

  2012cites this paper
• Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmid

  2012cites this paper
• The dityrosine cross-link as an intrinsic donor for assembling FRET pairs in the study of protein structure.

  2012cites this paper
• Mecanismo de inactivación catabólica de las proteínas de membrana plasmática de Sccharomyces cerevisiae

  2012cites this paper