Effects of medium composition on the morphology and function of rat hepatocytes cultured as spheroids and monolayers

No abstract is available for this paper.

• Publication year

  2001

• Venue

  In Vitro Cellular & Developmental Biology - Animal

• Publication date

  2001-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1290/1071-2690(2001)037<0656:EOMCOT>2.0.CO;2PMID 11776971
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

  2010cited by this paper
• Structural and functional features of bile canaliculi in adult rat hepatocyte spheroids.

  2008cited by this paper
• Attachment and long term survival of adult rat hepatocytes in primary monolayer cultures: Comparison of different substrata and tissue culture media formulations

  2007cited by this paper
• Amino acid and energy requirements for rat hepatocytes in primary culture

  2007cited by this paper
• Amino acid and energy requirements for rat hepatocytes in primary culture

  2007cited by this paper
• The use of liver spheroids as an in vitro model for studying induction of the stress response as a marker of chemical toxicity.

  2000cited by this paper
• Serum-free, long-term cultures of human hepatocytes: maintenance of cell morphology, transcription factors, and liver-specific functions.

  2000cited by this paper
• The differential cytotoxicity of methotrexate in rat hepatocyte monolayer and spheroid cultures.

  2000cited by this paper
• The use of Long-term Hepatocyte Cultures for Detecting Induction of Drug Metabolising Enzymes: The Current Status

  1999cited by this paper
• Progressive maturation resistance to microcystin-LR cytotoxicity in two different hepatospheroidal models

  1999cited by this paper
• Formation of actin filament networks in cultured rat hepatocytes treated with DMSO and glucagon.

  1997cited by this paper
• Maintenance of cytochrome P450 content and phase I and phase II enzyme activities in trout hepatocytes cultured as spheroidal aggregates

  1996cited by this paper
• Efficient assembly of rat hepatocyte spheroids for tissue engineering applications

  1996cited by this paper
• Large-Scale Preparation and Function of Porcine Hepatocyte Spheroids

  1996cited by this paper
• Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids

  1996cited by this paper
• Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes

  1996cited by this paper
• An improved HPLC assay for the assessment of liver slice metabolic viability using 7-ethoxycoumarin.

  1995cited by this paper
• Hepatocyte-based in vitro models and their application in pharmacotoxicology Report of an EC DGXII meeting with representatives of the European pharmaceutical industry.

  1995cited by this paper
• Modulation of xenobiotic-inducible cytochrome P450 gene expression by dexamethasone in primary rat hepatocytes.

  1995cited by this paper
• The influence of medium composition on the maintenance of cytochrome P-450, glutathione content and urea synthesis: a comparison of rat and sheep primary hepatocyte cultures.

  1995cited by this paper
• Culture of Animal Cells: A Manual of Basic Technique, R. Ian Freshney (Ed.). Wiley-Liss, Inc., New York (1994)

  1995cited by this paper
• A morphological study of differentiated hepatocytes in vitro

  1995cited by this paper
• Collagen gel immobilization: a useful cell culture technique for long-term metabolic studies on human hepatocytes.

  1994cited by this paper
• Effect of culture medium on the maintenance of steroid metabolism in cultured adult rat hepatocytes

  1994cited by this paper
• A new three‐dimensional culture system for hepatocytes using reticulated polyurethane

  1994cited by this paper
• An alternative to hepatotoxicity testing using multicellular spheroids composed of rat liver cells

  1994cited by this paper
• Hepatocyte Spheroids: Prolonged Hepatocyte Viability for in Vitro Modeling of Nongenotoxic Carcinogenesis

  1993cited by this paper
• Factors Affecting Secretory Protein Production in Primary Cultures of Rat Hepatocytes

  1993cited by this paper
• Hepatocyte spheroids: prolonged hepatocyte viability for in vitro modeling of nongenotoxic carcinogenesis.

  1993cited by this paper
• Regulation of RNA degradation in cultured rat hepatocytes: Effects of specific amino acids and insulin

  1993cited by this paper
• Hepatocyte Aggregate Culture Technique for Bioreactors in Hybrid Liver Support Systems

  1993cited by this paper
• Reestablishment of cell polarity of rat hepatocytes in primary culture

  1993cited by this paper
• The importance of proline on long‐term hepatocyte function in a collagen gel sandwich configuration: Regulation of protein secretion

  1992cited by this paper
• Long-term culture of adult rat hepatocyte spheroids.

  1992cited by this paper
• A simplified method for the culturing of primary adult rat and human hepatocytes as multicellular spheroids

  1992cited by this paper
• Rat hepatocytes cultured on a monkey kidney cell line: Expression of biotransformation and hepatic metabolic activities.

  1991cited by this paper
• Long‐Term in Vitro Function of Adult Hepatocytes in a Collagen Sandwich Configuration

  1991cited by this paper
• Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments.

  1990cited by this paper
• A Diagnostic Programme for Quantitative Analysis of Proteinuria

  1989cited by this paper
• Immunocytochemical localization of myosin in normal and phalloidin-treated rat hepatocytes.

  1989cited by this paper
• Hepatotoxicity and molecular aspects of hepatocyte function in primary culture.

  1988cited by this paper
• Regulation of albumin synthesis by hormones and amino acids in primary cultures of rat hepatocytes.

  1987cited by this paper
• Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities

  1985cited by this paper
• Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.

  1985cited by this paper
• Direct effect of insulin on albumin gene expression in primary cultures of rat hepatocytes.

  1985cited by this paper
• Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type.

  1983cited by this paper
• Insulin inhibition of protein degradation in cell monolayers

  1980cited by this paper
• Amino acid inhibition of the autophagic/lysosomal pathway of protein degradation in isolated rat hepatocytes.

  1980cited by this paper
• [5] Media and growth requirements

  1979cited by this paper
• Molecular properties of the Busycon hemocyanin--SDS complex with reference to its usefulness as a high molecular weight standard.

  1978cited by this paper
• A fluorometric method for determination of oxidized and reduced glutathione in tissues.

  1976cited by this paper
• Conditions affecting primary cell cultures of functional adult rat hepatocytes

  1976cited by this paper
• Conditions affecting primary cell cultures of functional adult rat hepatocytes

  1976cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Preparation of rat liver cells. II. Effects of ions and chelators on tissue dispersion.

  1973cited by this paper

• Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces

  2025cites this paper
• Advancing osteosarcoma 3D modeling in vitro for novel tumor microenvironment-targeted therapies development.

  2024cites this paper
• Assessing Receptor Activation in 2D and 3D Cultured Hepatocytes: Responses to a Single Compound and a Complex Mixture

  2024cites this paper
• A Step Forward in the Characterization of Primary Brown Trout Hepatocytic Spheroids as Experimental Models

  2023cites this paper
• Advances in Recapitulating Alzheimer’s Disease Phenotypes Using Human Induced Pluripotent Stem Cell-Based In Vitro Models

  2022cites this paper
• Concentration‐ and time‐dependent induction of Cyp1a and DNA damage response by benzo(a)pyrene in LMH three‐dimensional spheroids

  2021cites this paper
• Treatment with 188Re Reduces Cancerous Phenotype in Liver Cancer Cells

  2021cites this paper
• Demonstration of the first‐pass metabolism in the skin of the hair dye, 4‐amino‐2‐hydroxytoluene, using the Chip2 skin–liver microphysiological model

  2021cites this paper
• Microfluidic technologies to engineer mesenchymal stem cell aggregates—applications and benefits

  2020cites this paper
• Recent Advances in Three-Dimensional Multicellular Spheroid Formation for Biomedical Research

  2020cites this paper
• Leveraging and manufacturing in vitro multicellular spheroid-based tumor cell model as a preclinical tool for translating dysregulated tumor metabolism into clinical targets and biomarkers

  2020cites this paper
• Evaluation of the Aryl Hydrocarbon Receptor Response in LMH 3D Spheroids

  2020cites this paper
• On‐Demand Coalescence and Splitting of Liquid Marbles and Their Bioapplications

  2019cites this paper
• Anticancer drug discovery using multicellular tumor spheroid models

  2019cites this paper
• Challenges and Opportunities in the Design of Liver-on-Chip Microdevices.

  2019cites this paper
• Cleavable cellulosic sponge for functional hepatic cell culture and retrieval.

  2019cites this paper
• Tuning microenvironment for multicellular spheroid formation in thermo-responsive anionic microgel scaffolds.

  2018cites this paper
• A novel approach to producing uniform 3-D tumor spheroid constructs using ultrasound treatment

  2018cites this paper
• Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells

  2018cites this paper
• A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries.

  2018cites this paper
• Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis

  2018cites this paper
• Liver-on-a-chip for evaluating hepatic activation of clopidogrel in patients with coronary stents

  2017cites this paper
• Microenvironment of a tumor-organoid system enhances hepatocellular carcinoma malignancy-related hallmarks

  2017cites this paper
• A mechanistic study on tumour spheroid formation in thermosensitive hydrogels: Experiments and mathematical modelling

  2016cites this paper
• Investigating Nonalcoholic Fatty Liver Disease in a Liver-on-a-Chip Microfluidic Device

  2016cites this paper
• Three-dimensional Organotypic Culture Models of Human Hepatocellular Carcinoma

  2016cites this paper
• Thermoresponsive Microgels for Multicellular Spheroids Formation

  2016cites this paper
• Tissue engineering of the liver

  2016cites this paper
• Superiority of three-dimensional stem cell clusters over monolayer culture: An archetype to biological application

  2016cites this paper
• Development and evaluation of a novel approach to producing uniform 3-D tumor spheroid constructs

  2016cites this paper
• Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models

  2015cites this paper
• Cancer cell spheroids for screening of chemotherapeutics and drug-delivery systems.

  2015cites this paper
• Organ-on-a-Chip: New Platform for Biological Analysis

  2015cites this paper
• 3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip.

  2015cites this paper
• Micropatterned Cell–Cell Interactions Enable Functional Encapsulation of Primary Hepatocytes in Hydrogel Microtissues

  2014cites this paper
• Generation of a multi-organ-chip-based liver equivalent for toxicity testing

  2014cites this paper
• Serum‐free spheroid suspension culture maintains mesenchymal stem cell proliferation and differentiation potential

  2014cites this paper
• Higher Expressions of Cytochrome P450, UDP-Glucuronosyltransferase,and Transporter Genes in Nanopillar-Cultured Rat Hepatocyte Spheroids

  2013cites this paper
• Effects of Culture Conditions on a Micropatterned Co-culture of Rat Hepatocytes with 3T3 cells

  2013cites this paper
• Reversal of the multidrug resistance by drug combination using multifunctional liposomes

  2013cites this paper
• Towards a more representative in vitro method for fish ecotoxicology: morphological and biochemical characterisation of three-dimensional spheroidal hepatocytes

  2012cites this paper
• The gap between cell and animal models: nanoparticle drug-delivery development and characterization using microtissue models.

  2012cites this paper
• Three-Dimensional Spheroid Culture: A Promising Tool for Cancer Research

  2012cites this paper
• Effect of vitrifying solutions, ethylene glycol and 1, 2-propandiol, on intracellular K+ and lactate dehydrogenase (LDH) of hepatocytes in primary culture

  2012cites this paper
• Organotypic systems in drug metabolism and toxicity: challenges and opportunities

  2012cites this paper
• Aggregation-promoted expansion of neuraly committed human umbilical cord blood progenitors in vitro.

  2011cites this paper
• Issues in Bioartificial Liver Support Therapy for Acute Liver Failure

  2011cites this paper
• SREBP isoform and SREBP target gene expression during rat primary hepatocyte culture

  2010cites this paper
• Formation of Hepatocyte Spheroids with Structural Polarity and Functional Bile Canaliculi Using Nanopillar Sheets

  2010cites this paper
• An investigation of Cytochrome P450 function in human cell Lines for potential use in a bioartificial liver device

  2010cites this paper
• Microsphere-mediated control of embryoid body microenvironments

  2010cites this paper
• 3D automatic quantification applied to optically sectioned images to improve microscopy analysis.

  2009cites this paper
• Modulation of hepatocyte phenotype in vitro via chemomechanical tuning of polyelectrolyte multilayers.

  2009cites this paper
• A two-fluid model for tissue growth within a dynamic flow environment

  2008cites this paper
• Recent advances in three‐dimensional multicellular spheroid culture for biomedical research

  2008cites this paper
• Multiphase modelling of tissue growth in dynamic culture conditions

  2008cites this paper
• Bioartificial liver systems: why, what, whither?

  2008cites this paper
• Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells.

  2007cites this paper
• An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture.

  2007influential citation
• Rotary Suspension Culture Enhances the Efficiency, Yield, and Homogeneity of Embryoid Body Differentiation

  2007cites this paper
• In Vitro Matrices for Studying Tumor Cell Invasion

  2006cites this paper
• Cell Motility in Cancer Invasion and Metastasis

  2006cites this paper
• Rat Hepatocyte Cultures

  2006cites this paper
• Compositions cellulaires et leurs procédés de préparation

  2006cites this paper
• Compositions cellulaires et procédé permettant de les préparer

  2006cites this paper
• Actin organization and hepatocyte differentiation are regulated by extracellular matrix via PI‐4,5‐bisphosphate in the rat

  2006cites this paper
• Integration of technologies for hepatic tissue engineering.

  2006cites this paper
• Primary human hepatocytes in spheroid formation to study hepatitis C infection.

  2006cites this paper
• Rat Hepatocyte Cultures : Conventional Monolayer and Cocultures With Rat Liver Epithelial Cells.

  2006cites this paper
• Dexamethasone Effects on Rat Hepatocyte Spheroid Formation and Function

  2005cites this paper
• Hybrid artificial liver support system for treatment of severe liver failure.

  2005cites this paper
• Impact of dimethyl sulfoxide on expression of nuclear receptors and drug-inducible cytochromes P450 in primary rat hepatocytes.

  2004cites this paper
• Development of Co-Culture System of Hepatocytes with Bone Marrow Cells for Expression and Maintenance of Hepatic Functions

  2004cites this paper
• Ultrastructural and Functional Investigations of Adult Hepatocyte Spheroids during in Vitro Cultivation

  2004cites this paper
• Altered mitochondrial function and cholesterol synthesis influences protein synthesis in extended HepG2 spheroid cultures.

  2004cites this paper
• Membrane-Based PDMS Microbioreactor for Perfused 3D Primary Rat Hepatocyte Cultures

  2004cites this paper
• Hepatocyte Culture as an In Vitro Model for Evaluating the Hepatotoxicity of Food-Borne Toxicants and Microbial Pathogens: A Review

  2003cites this paper
• Generation of confluent cardiomyocyte monolayers derived from embryonic stem cells in suspension: a cell source for new therapies and screening strategies.

  2003cites this paper