Ex vivo expansion of hematopoietic stem cells by cytokines.

Autologous or allogeneic hematopoietic stem cell (HSC) transplantation has been used successfully in the treatment of hematological diseases, neoplasias, primary immunodeficiency diseases, and metabolic disorders of children and adult patients. Historically, the bone marrow (BM) has represented the main source of HSCs in pediatric and adult individuals. However, in many cases, a suitable donor is hardly found, thus limiting the applicability of this lifesaving treatment. This difficulty has led to a search for alternative sources of HSCs for use in human transplantations. Two sources of human HSCs have been identified: cord blood (CB) and peripheral blood (PB). PB is considered to be practical sources of HSCs, especially because methods exist which can mobilize significant numbers of stem/progenitor cells into circulation after administration of G-CSF. However, the poor mobilization happens sometimes, and more importantly, concerns about donor safety have been apprehended because of splenic rupture during GCSF application [1]. HSCs derived from CB have many advantages for transplantation, but the recipient is restricted to pediatric patients because of its small volume. These inconveniences have been considered to be resolved if ex vivo expansion of HSCs would turn out to reality. Recently, the identification, cloning, and production of recombinant cytokines together with the identification and purification of HSCs and progenitor cells have greatly increased our understanding of the hematopoietic system. As a result of these fundamental discoveries, many investigators are now attempting ex vivo manipulation of HSCs for potential therapeutic purposes. In general, the objectives of these manipulations are to expand HSCs responsible for long-term hematopoietic repopulation. This review will summarize the present understandings of the roles of cytokines for expansion of human HSCs, together with the development of assay systems to reflect human HSC activity correctly. Moreover, therapeutic applications for ex vivo expanded HSCs will also be discussed [2–4].

• Publication year

  2002

• Venue

  Biochimica et Biophysica Acta

• Publication date

  2002-11-11

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S0167-4889(02)00324-5PMID 12421675
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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