Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice

The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.

• Publication year

  2016

• Venue

  Scientific Reports

• Publication date

  2016-06-06

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/srep27351PMID 27272971PMCID 4894368
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mitofusin 2 ablation increases endoplasmic reticulum–mitochondria coupling

  2015cited by this paper
• Mitochondrial lipid trafficking.

  2014cited by this paper
• MAM (mitochondria-associated membranes) in mammalian cells: lipids and beyond.

  2014cited by this paper
• Mitochondrial form and function

  2014cited by this paper
• General statistical framework for quantitative proteomics by stable isotope labeling.

  2014cited by this paper
• Mitochondria-Associated Endoplasmic Reticulum Membrane (MAM) Integrity Is Required for Insulin Signaling and Is Implicated in Hepatic Insulin Resistance

  2014cited by this paper
• The endoplasmic reticulum-mitochondria connection: one touch, multiple functions.

  2014cited by this paper
• The human HDL proteome displays high inter-individual variability and is altered dynamically in response to angioplasty-induced atheroma plaque rupture.

  2014cited by this paper
• New functions of mitochondria associated membranes in cellular signaling.

  2014cited by this paper
• Mitochondria-associated ER membranes (MAMs) and glycosphingolipid enriched microdomains (GEMs): isolation from mouse brain.

  2013cited by this paper
• Caveolin-1 is necessary for hepatic oxidative lipid metabolism: evidence for crosstalk between caveolin-1 and bile acid signaling.

  2013cited by this paper
• Mitochondria-associated ER membranes in Alzheimer disease.

  2013cited by this paper
• Caveolae as plasma membrane sensors, protectors and organizers

  2013cited by this paper
• Upregulated function of mitochondria-associated ER membranes in Alzheimer disease

  2012cited by this paper
• Intrahepatic role of exchangeable apolipoproteins in lipoprotein assembly and secretion.

  2012cited by this paper
• A Robust Method for Quantitative High-throughput Analysis of Proteomes by 18O Labeling*

  2011cited by this paper
• Caveolin-1 deficiency causes cholesterol-dependent mitochondrial dysfunction and apoptotic susceptibility.

  2011cited by this paper
• Mitochondrial Cholesterol: A Connection Between Caveolin, Metabolism, and Disease

  2011cited by this paper
• Myelination transition zone astrocytes are constitutively phagocytic and have synuclein dependent reactivity in glaucoma

  2011cited by this paper
• A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

  2010cited by this paper
• Imaging interorganelle contacts and local calcium dynamics at the ER-mitochondrial interface.

  2010cited by this paper
• Universal sample preparation method for proteome analysis

  2009cited by this paper
• Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells

  2009cited by this paper
• Major Urinary Protein-1 Increases Energy Expenditure and Improves Glucose Intolerance through Enhancing Mitochondrial Function in Skeletal Muscle of Diabetic Mice*

  2009cited by this paper
• GM1-ganglioside accumulation at the mitochondria-associated ER membranes links ER stress to Ca(2+)-dependent mitochondrial apoptosis.

  2009cited by this paper
• Role of caveolin-1 in the regulation of lipoprotein metabolism.

  2008cited by this paper
• Properties of Average Score Distributions of SEQUEST

  2008cited by this paper
• Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

  2008cited by this paper
• Identification and Characterization of Associated with Lipid Droplet Protein 1: A Novel Membrane‐Associated Protein That Resides on Hepatic Lipid Droplets

  2006cited by this paper
• Opinion: Lipid droplets: a unified view of a dynamic organelle

  2006cited by this paper
• Loss of Caveolae, Vascular Dysfunction, and Pulmonary Defects in Caveolin-1 Gene-Disrupted Mice

  2001cited by this paper

• Identification of Central Genes in the Pathogenesis of Lipid Metabolism in Chronic Lymphocytic Leukaemia: A Multi-Omics Study Integrating Machine Learning and Mendelian Randomisation.

  2026cites this paper
• Mitochondria–ER crosstalk via MAMS: Bridging cellular homeostasis and cancer progression

  2026cites this paper
• Natural products modulate mitochondria-associated membranes to prevent cardiovascular diseases.

  2026cites this paper
• Role of mitochondria-associated membranes (MAMs) in inflammatory signaling: implications for acute lung injury pathogenesis

  2025cites this paper
• Exploring the interplay between mitochondria and endoplasmic reticulum in pulmonary arterial hypertension

  2025cites this paper
• Orchestrating movement: the role of Caveolin-1 in migration and metastasis

  2025cites this paper
• Regulation of endoplasmic reticulum–mitochondria transfer and its importance in GBM

  2025cites this paper
• Mitochondria‐Associated Endoplasmic Reticulum Membranes in Health and Diseases

  2025cites this paper
• Schisandra chinensis mixture attenuates diabetic kidney disease via VDAC1/Grp75/IP3R-mediated MAMs stabilization and apoptosis-autophagy regulation.

  2025cites this paper
• Endoplasmic reticulum-mediated organelle crosstalk in kidney disease

  2025cites this paper
• The role of mitochondria-endoplasmic reticulum crosstalk in colorectal cancer

  2025cites this paper
• Revisiting the role of Annexins in membrane trafficking

  2025cites this paper
• FMO2 Prevents Pathological Cardiac Hypertrophy by Maintaining the ER-Mitochondria Association Through Interaction With IP3R2-Grp75-VDAC1

  2025cites this paper
• Mitochondria‐Associated Membranes: A Key Point of Neurodegenerative Diseases

  2025cites this paper
• Biophysical characterization of anion channels in Mitochondrion-Endoplasmic-Reticulum Contact sites (MERCs).

  2025cites this paper
• MAM kinases: physiological roles, related diseases, and therapeutic perspectives—a systematic review

  2025cites this paper
• The perspective of modern transplant science – transplant arteriosclerosis: inspiration derived from mitochondria associated endoplasmic reticulum membrane dysfunction in arterial diseases

  2025cites this paper
• Mitochondria associated membranes in dilated cardiomyopathy: connecting pathogenesis and cellular dysfunction

  2025cites this paper
• The fundamental role of mitochondria–endoplasmic reticulum contacts in ageing and declining healthspan

  2025cites this paper
• CPT1A-mediated MFF succinylation promotes stemness maintenance in ovarian cancer stem cells

  2025cites this paper
• Mitochondrial Calcium Signaling in Hepatocyte Health and Disease.

  2025cites this paper
• The role of mitochondrial-associated endoplasmic reticulum membranes (MAMs) in diabetic microvascular complications: a review

  2025cites this paper
• A new perspective on liver diseases: focusing on the mitochondria-associated endoplasmic reticulum membranes.

  2024cites this paper
• The contribution of mitochondria-associated ER membranes to cholesterol homeostasis

  2024cites this paper
• Endoplasmic Reticulum Stress in Bronchopulmonary Dysplasia: Contributor or Consequence?

  2024cites this paper
• Mechanisms of drug resistance in nutrient-depleted colorectal cancer cells: insights into lysosomal and mitochondrial drug sequestration

  2024cites this paper
• Current Insights on Mitochondria-Associated Endoplasmic Reticulum Membranes (MAMs) and their significance in the pathophysiology of ocular disorders.

  2024cites this paper
• Unraveling the complex interplay between Mitochondria-Associated Membranes (MAMs) and cardiovascular Inflammation: Molecular mechanisms and therapeutic implications.

  2024cites this paper
• Role of mitochondria‐endoplasmic reticulum contacts in neurodegenerative, neurodevelopmental and neuropsychiatric conditions

  2024cites this paper
• Targeting protein interaction networks in mitochondrial dynamics for neurodegenerative diseases

  2024cites this paper
• Mitochondrial structure and function: a new direction for the targeted treatment of chronic liver disease with Chinese herbal medicine.

  2024cites this paper
• The gluconeogenesis enzyme PCK2 has a non-enzymatic role in proteostasis in endothelial cells

  2024cites this paper
• Mitochondria-associated endoplasmic reticulum membranes as a therapeutic target for cardiovascular diseases

  2024cites this paper
• Deciphering mitochondrial dysfunction: Pathophysiological mechanisms in vascular cognitive impairment.

  2024cites this paper
• Interplay of mitochondria associated membrane proteins and autophagy: Implications in neurodegeneration.

  2024cites this paper
• Mitochondria-associated endoplasmic reticulum membrane (MAM): a dark horse for diabetic cardiomyopathy treatment

  2024influential citation
• Identification of myoferlin as a mitochondria-associated membranes component required for calcium signaling in PDAC cell lines

  2024cites this paper
• Broadening horizons: the contribution of mitochondria-associated endoplasmic reticulum membrane (MAM) dysfunction in diabetic kidney disease

  2023cites this paper
• Mitochondria-associated endoplasmic reticulum membranes (MAMs) and their role in glaucomatous retinal ganglion cell degeneration—a mini review

  2023cites this paper
• Novel tumor therapy strategies targeting endoplasmic reticulum-mitochondria signal pathways.

  2023cites this paper
• Hakuna MAM-Tata: Investigating the role of mitochondrial-associated membranes in ALS.

  2023cites this paper
• Ca2+ and Annexins - Emerging Players for Sensing and Transferring Cholesterol and Phosphoinositides via Membrane Contact Sites.

  2023cites this paper
• Caveolae Mechanotransduction at the Interface between Cytoskeleton and Extracellular Matrix

  2023cites this paper
• Key genes expressed in mitochondria‑endoplasmic reticulum contact sites in cancer (Review)

  2023cites this paper
• Mitochondria-Associated Endoplasmic Reticulum Membrane (MAM) Is a Promising Signature to Predict Prognosis and Therapies for Hepatocellular Carcinoma (HCC)

  2023influential citation
• Synergistic mechanism between the endoplasmic reticulum and mitochondria and their crosstalk with other organelles

  2023influential citation
• Cholesterol Redistribution in Pancreatic β-Cells: A Flexible Path to Regulate Insulin Secretion

  2023cites this paper
• Mitochondria-associated endoplasmic reticulum membranes (MAMs): Possible therapeutic targets in heart failure

  2023cites this paper
• DsbA-L: A Promising Therapeutic Target for Metabolic Diseases.

  2023cites this paper
• Novel tumor therapy strategies targeting endoplasmic reticulum-mitochondria signal pathwaysRunning title_ Tumor therapy based on ER-mitochondria interactions

  2023cites this paper
• TRPV1 Channels Are New Players in the Reticulum–Mitochondria Ca2+ Coupling in a Rat Cardiomyoblast Cell Line

  2023cites this paper
• The role of endoplasmic reticulum-mitochondria-associated membranes in diabetic kidney disease.

  2023cites this paper
• Mitochondrial Dysfunction and the Glycolytic Switch Induced by Caveolin-1 Phosphorylation Promote Cancer Cell Migration, Invasion, and Metastasis

  2022cites this paper
• Compositions and Functions of Mitochondria-Associated Endoplasmic Reticulum Membranes and Their Contribution to Cardioprotection by Exercise Preconditioning

  2022cites this paper
• Caveolin-1 controls mitochondrial damage and ROS production by regulating fission - fusion dynamics and mitophagy

  2022cites this paper
• Endoplasmic Reticulum-Mitochondria Contacts Modulate Reactive Oxygen Species-Mediated Signaling and Oxidative Stress in Brain Disorders: The Key Role of Sigma-1 Receptor

  2022cites this paper
• Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle

  2022cites this paper
• Endoplasmic reticulum-mitochondria signaling in neurons and neurodegenerative diseases.

  2022cites this paper
• Get Closer to the World of Contact Sites: A Beginner's Guide to Proximity-Driven Fluorescent Probes

  2022cites this paper
• Heterogeneity of the endoplasmic reticulum Ca2+ store determines colocalization with mitochondria.

  2022cites this paper
• Mitochondria-endoplasmic reticulum contacts in sepsis-induced myocardial dysfunction

  2022cites this paper
• The regulation and functions of ACSL3 and ACSL4 in the liver and hepatocellular carcinoma

  2022cites this paper
• Liver mitochondria-associated endoplasmic reticulum membrane proteomics for studying the effects of ZiBuPiYin recipe on Zucker diabetic fatty rats after chronic psychological stress

  2022cites this paper
• Caveolin-1 Signaling-Driven Mitochondrial Fission and Cytoskeleton Remodeling Promotes Breast Cancer Migration.

  2022cites this paper
• UPRmt and coordinated UPRER in type 2 diabetes

  2022cites this paper
• Proteomic Analysis of Retinal Mitochondria-Associated ER Membranes Identified Novel Proteins of Retinal Degeneration in Long-Term Diabetes

  2022cites this paper
• Annexins Bridging the Gap: Novel Roles in Membrane Contact Site Formation

  2022influential citation
• The Adhesion GPCR VLGR1/ADGRV1 Regulates the Ca2+ Homeostasis at Mitochondria-Associated ER Membranes

  2022cites this paper
• Functional diversity of apolipoprotein E: from subcellular localization to mitochondrial function

  2022cites this paper
• Caveolin‐1 influences mitochondrial plasticity and function in hepatic stellate cell activation

  2022cites this paper
• Miro proteins and their role in mitochondrial transfer in cancer and beyond

  2022cites this paper
• Insight into Liver lncRNA and mRNA Profiling at Four Developmental Stages in Ningxiang Pig

  2021cites this paper
• Mitochondria Associated Membranes (MAMs): Architecture and physiopathological role.

  2021cites this paper
• Mitochondria-Associated Endoplasmic Reticulum Membranes in Breast Cancer

  2021cites this paper
• ER Stress-Sensor Proteins and ER-Mitochondrial Crosstalk—Signaling Beyond (ER) Stress Response

  2021cites this paper
• Role of Sigma-1 Receptor in Calcium Modulation: Possible Involvement in Cancer

  2021cites this paper
• Targeting Lipid Rafts as a Strategy Against Coronavirus

  2021cites this paper
• Perspectives on Organelle Interaction, Protein Dysregulation, and Cancer Disease

  2021cites this paper
• Oleic Acid and Eicosapentaenoic Acid Reverse Palmitic Acid-induced Insulin Resistance in Human HepG2 Cells via the Reactive Oxygen Species/JUN Pathway

  2021cites this paper
• Mind the Gap: Mitochondria and the Endoplasmic Reticulum in Neurodegenerative Diseases

  2021cites this paper
• Mitochondrial associated ER membrane (MAM) compartment and its dysregulation in Amyotrophic lateral sclerosis (ALS).

  2021cites this paper
• Endoplasmic reticulum stress and muscle dysfunction in congenital Lipodystrophies.

  2021cites this paper
• Calcium-dependent insulin resistance in hepatocytes: mathematical model.

  2021cites this paper
• TCAIM controls effector T cell generation by preventing Mitochondria-Endoplasmic Reticulum Contact Site-initiated Cholesterol Biosynthesis

  2021cites this paper
• Post-Translational Modification of Cysteines: A Key Determinant of Endoplasmic Reticulum-Mitochondria Contacts (MERCs)

  2021cites this paper
• Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes

  2021cites this paper
• The ER-mitochondria Ca2+ signaling in cancer progression: Fueling the monster.

  2021cites this paper
• Miro proteins connect mitochondrial function and intercellular transport

  2021cites this paper
• Identification of Interorganelle Crosstalk Effect on Various Diseases, Including Kidney Disease: A Systematic Review and Meta-analysis

  2021cites this paper
• Molecular Dysfunctions of Mitochondria-Associated Endoplasmic Reticulum Contacts in Atherosclerosis

  2021cites this paper
• Loss and gain of function of Grp75 or mitofusin 2 distinctly alter cholesterol metabolism, but all promote triglyceride accumulation in hepatocytes.

  2021cites this paper
• Balancing life and death: BCL‐2 family members at diverse ER–mitochondrial contact sites

  2021cites this paper
• The Mitochondrial-Associated Endoplasmic Reticulum Membrane and Its Role in Diabetic Nephropathy

  2021cites this paper
• Caveolin-1 Regulates Cellular Metabolism: A Potential Therapeutic Target in Kidney Disease

  2021cites this paper
• Caveolin‐3 deficiency associated with the dystrophy P104L mutation impairs skeletal muscle mitochondrial form and function

  2020cites this paper
• Mitochondria-Associated Membranes (MAMs): A Novel Therapeutic Target for Treating Metabolic Syndrome.

  2020cites this paper
• The caveolar-mitochondrial interface: regulation of cellular metabolism in physiology and pathophysiology.

  2020cites this paper
• Mitochondrial Membrane Intracellular Communication in Healthy and Diseased Myocardium

  2020cites this paper
• ER-mitochondria contact sites in neurodegeneration: genetic screening approaches to investigate novel disease mechanisms

  2020cites this paper
• A Role for Caveolin-3 in the Pathogenesis of Muscular Dystrophies

  2020cites this paper