Effects of Indole-3-Acetic Acid on the Transcriptional Activities and Stress Tolerance of Bradyrhizobium japonicum

A genome-wide transcriptional profile of Bradyrhizobium japonicum, the nitrogen-fixing endosymbiont of the soybean plant, revealed differential expression of approximately 15% of the genome after a 1 mM treatment with the phytohormone indole-3-acetic acid (IAA). A total of 1,323 genes were differentially expressed (619 up-regulated and 704 down-regulated) at a two-fold cut off with q value ≤ 0.05. General stress response genes were induced, such as those involved in response to heat, cold, oxidative, osmotic, and desiccation stresses and in exopolysaccharide (EPS) biosynthesis. This suggests that IAA is effective in activating a generalized stress response in B. japonicum. The transcriptional data were corroborated by the finding that stress tolerance of B. japonicum in cell viability assays was enhanced when pre-treated with 1 mM IAA compared to controls. The IAA treatment also stimulated biofilm formation and EPS production by B. japonicum, especially acidic sugar components in the total EPS. The IAA pre-treatment did not influence the nodulation ability of B. japonicum. The data provide a comprehensive overview of the potential transcriptional responses of the symbiotic bacterium when exposed to the ubiquitous hormone of its plant host.

• Publication year

  2013

• Venue

  PLoS ONE

• Publication date

  2013-10-02

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0076559PMID 24098533PMCID 3788728
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2008cited by this paper
• Comprehensive transcriptome analysis of auxin responses in Arabidopsis.

  2008cited by this paper
• Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L.)

  2008cited by this paper
• Auxin and nitric oxide control indeterminate nodule formation

  2007cited by this paper
• An oligonucleotide microarray resource for transcriptional profiling of Bradyrhizobium japonicum.

  2007cited by this paper
• Transcriptional and Physiological Responses of Bradyrhizobium japonicum to Desiccation-Induced Stress

  2007cited by this paper
• Phytohormone production by three strains of Bradyrhizobium japonicum and possible physiological and technological implications

  2007cited by this paper
• Alginate Production by Pseudomonas putida Creates a Hydrated Microenvironment and Contributes to Biofilm Architecture and Stress Tolerance under Water-Limiting Conditions

  2007cited by this paper
• Exopolysaccharides from Burkholderia cenocepacia Inhibit Neutrophil Chemotaxis and Scavenge Reactive Oxygen Species*

  2006cited by this paper
• Indole-3-acetic acid improves Escherichia coli’s defences to stress

  2006cited by this paper
• Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli.

  2006cited by this paper
• Auxin in action: signalling, transport and the control of plant growth and development

  2006cited by this paper
• Crucial Role of Extracellular Polysaccharides in Desiccation and Freezing Tolerance in the Terrestrial Cyanobacterium Nostoc commune

  2005cited by this paper
• Genome-Wide Transcriptional Profiling of the Steady-State Response of Pseudomonas aeruginosa to Hydrogen Peroxide

  2005cited by this paper
• Colorimetric Method for Identifying Plant Essential Oil Components That Affect Biofilm Formation and Structure

  2004cited by this paper
• Reduced Water Availability Influences the Dynamics, Development, and Ultrastructural Properties of Pseudomonas putida Biofilms

  2003cited by this paper
• DNA Microarrays: A Molecular Cloning Manual.

  2003cited by this paper
• Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.

  2003cited by this paper
• Plant biology: Locks, keys and symbioses

  2003cited by this paper
• DNA Microarrays: A Molecular Cloning Manual

  2002cited by this paper
• Microtiter Plate Assay for Assessment of Listeria monocytogenes Biofilm Formation

  2002cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• In silico prediction of protein-protein interactions in human macrophages

  2001cited by this paper
• Structure and carbohydrate analysis of the exopolysaccharide capsule of Pseudomonas putida G7.

  2001cited by this paper
• New method for exopolysaccharide determination in culture broth using stirred ultrafiltration cells

  2001cited by this paper
• Biosynthesis of indole-3-acetic acid in Azospirillum brasilense. Insights from quantum chemistry.

  2001cited by this paper
• Keys to Symbiotic Harmony

  2000cited by this paper
• Analysis of Variance for Gene Expression Microarray Data

  2000cited by this paper
• A high-affinity cbb3-type cytochrome oxidase terminates the symbiosis-specific respiratory chain of Bradyrhizobium japonicum

  1996cited by this paper
• Catabolism of indole-3-acetic acid and 4- and 5-chloroindole-3-acetic acid in Bradyrhizobium japonicum

  1995cited by this paper
• Indolepyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. A new enzyme involved in peptide fermentation.

  1994cited by this paper
• Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis.

  1993cited by this paper
• The role of auxin in plant-disease development.

  1993cited by this paper
• Molecular mechanisms of crown gall tumorigenesis

  1991cited by this paper
• Production of Indole-3-Acetic Acid by Bradyrhizobium japonicum: A Correlation with Genotype Grouping and Rhizobitoxine Production

  1991cited by this paper
• Oxygen-dependent catabolism of indole-3-acetic acid in Bradyrhizobium japonicum

  1991influential reference
• Cell Biology of Agrobacterium Infection and Transformation of Plants

  1988cited by this paper
• Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean

  1987cited by this paper
• Influence of 5-Methyltryptophan-Resistant Bradyrhizobium japonicum on Soybean Root Nodule Indole-3-Acetic Acid Content

  1987cited by this paper
• Purification and characterization of adhesive exopolysaccharides from Pseudomonas putida and Pseudomonas fluorescens.

  1987cited by this paper
• Insertion sequence elements of Pseudomonas savastanoi: Nucleotide sequence and homology with Agrobacterium tumefaciens transfer DNA.

  1986cited by this paper
• Effect of lectin on nodulation by wild-type Bradyrhizobium japonicum and a nodulation-defective mutant

  1986cited by this paper
• Stimulated nodulation of soybeans by Rhizobium japonicum mutant (B-14075) that catabolizes the conversion of tryptophan to indol-3yl-acetic acid

  1985cited by this paper
• Metabolite gene regulation of the L-arabinose operon in Escherichia coli with indoleacetic acid and other indole derivatives.

  1980cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• New method for quantitative determination of uronic acids.

  1973cited by this paper
• The Growth Of Rhizobium in Synthetic Media

  1961cited by this paper
• COLORIMETRIC METHOD FOR DETER-MINATION OF SUGAR AND RELATED SUBSTANCE

  1956cited by this paper
• Ueber das Verhalten der Skatolcarbonsäure im Organismus.

  year unknowncited by this paper

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  2026cites this paper
• MibR and LibR are involved in the transcriptional regulation of the ipdC gene in Azospirillum brasilense Sp7.

  2025cites this paper
• Long-term continuous cropping reshapes soybean rhizosphere microbiome and metabolome to alleviate allelopathic stress and enhance disease suppression

  2025cites this paper
• Auxin-mediated regulation of susceptibility to toxic metabolites, c-di-GMP levels, and phage infection in the rhizobacterium Serratia plymuthica

  2024cites this paper
• Systematic characterization of plant-associated bacteria that can degrade indole-3-acetic acid

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• Regulation of indole‐3‐acetic acid biosynthesis and consequences of auxin production deficiency in Serratia plymuthica

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• Excess boron stress and alleviation of its toxicity in plants: mechanisms and strategies

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• Transcriptome analysis of Armillaria gallica 012 m in response to auxin

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• Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress

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