Co-aggregation of penicillin g acylase and polyionic polymers: an easy methodology to prepare enzyme biocatalysts stable in organic media.

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Biomacromolecules

• Publication date

  2004-03-18

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1021/BM0343895PMID 15132672
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Overview of screening for new microbial catalysts and their uses in organic synthesis--selection and optimization of biocatalysts.

  2002cited by this paper
• Optimization of yield in kinetically controlled synthesis of ampicillin with immobilized penicillin acylase in organic media

  2002cited by this paper
• Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.

  2000cited by this paper
• Why are enzymes less active in organic solvents than in water?

  1997cited by this paper
• Dynamic reaction design of enzymic biotransformations in organic media: equilibrium‐controlled synthesis of antibiotics by penicillin G acylase

  1996cited by this paper
• Thermal inactivation of immobilized penicillin acylase in the presence of substrate and products

  1996cited by this paper
• Enzyme reaction engineering: synthesis of antibiotics catalysed by stabilized penicillin G acylase in the presence of organic cosolvents.

  1991cited by this paper

• Polyethylenimine-glutaraldehyde modification of Novozym® 435 enhances stability and sugar ester synthesis in a xylose-based natural deep eutectic solvent

  2026cites this paper
• Poly (Ethylene-Alt-Maleic Anhydride) Ionic Modification of Lipase B from Candida antarctica Immobilized on Octyl Agarose Beads Alters Its Catalytic Properties.

  2026cites this paper
• Orthogonal Valorization of 5‐Hydroxymethylfurfural Using Self‐Sufficient Heterogeneous Biocatalysts Composed of Two Co‐Immobilized Dehydrogenases

  2025cites this paper
• Statistically Assisted Preparation of Novel Alkaline Recombinant Crosslinked Enzyme Aggregates Lipase KV1 and Their Physiochemical Attributes

  2025cites this paper
• An Overview of Crosslinked Enzyme Aggregates: Concept of Development and Trends of Applications

  2024influential citation
• PEI-based functional materials: Fabrication techniques, properties, and biomedical applications.

  2024cites this paper
• Cationic Polymers Remarkably Boost Haloalkane Dehalogenase Activity in Organic Solvent Solutions and the Molecular Implications

  2023cites this paper
• Cross-linked enzyme aggregates immobilization: preparation, characterization, and applications

  2022cites this paper
• Enzyme immobilized nanomaterials

  2022cites this paper
• Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization.

  2022cites this paper
• Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies.

  2021cites this paper
• Employment of polysaccharides in enzyme immobilization

  2021cites this paper
• Immobilization of Pseudomonas stutzeri lipase through Cross-linking Aggregates (CLEA) for reactions in Deep Eutectic Solvents.

  2021cites this paper
• Entrapment of enzyme aggregates in chitosan beads for aroma release in white wines.

  2020cites this paper
• Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy

  2020cites this paper
• Tunable Polymeric Scaffolds for Enzyme Immobilization

  2020cites this paper
• Immobilized lipase-CLEA aggregates encapsulated in Lentikats® as robust biocatalysts for continuous processes in Deep Eutectic Solvents.

  2020cites this paper
• Entrapment of porous cross-linked enzyme aggregates of maltogenic amylase from Bacillus lehensis G1 into calcium alginate for maltooligosaccharides synthesis.

  2020cites this paper
• Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review

  2020cites this paper
• Pectin lyase immobilization using the glutaraldehyde chemistry increases the enzyme operation range.

  2020cites this paper
• Improvements in the production of Aspergillus oryzae β-galactosidase crosslinked aggregates and their use in repeated-batch synthesis of lactulose.

  2019cites this paper
• Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress

  2019cites this paper
• Preparation of lipase cross-linked enzyme aggregates in octyl-modified mesocellular foams.

  2019cites this paper
• Meso-molding three-dimensionally ordered macroporous alumina: A new platform to immobilize enzymes with high performance

  2019cites this paper
• CLEAs, Combi-CLEAs and ‘Smart’ Magnetic CLEAs: Biocatalysis in a Bio-Based Economy

  2019cites this paper
• Combi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor

  2019cites this paper
• Carbonic Anhydrase@ZIF-8 Hydrogel Composite Membrane with Improved Recycling and Stability for Efficient CO2 Capture.

  2019cites this paper
• Techniques for Preparation of Cross-Linked Enzyme Aggregates and Their Applications in Bioconversions

  2018cites this paper
• Optimization protocols and improved strategies for metal-organic frameworks for immobilizing enzymes: Current development and future challenges

  2018cites this paper
• Designing cross-linked xylanase aggregates for bioconversion of agroindustrial waste biomass towards potential production of nutraceuticals.

  2018cites this paper
• Remarkable stability of Candida antarctica lipase B immobilized via cross-linking aggregates (CLEA) in deep eutectic solvents

  2018cites this paper
• Preparation, characterization and optimization of cross-linked fructosyltransferase aggregates for the production of prebiotic fructooligosaccharides

  2018cites this paper
• Highly efficient production of chiral amines in batch and continuous flow by immobilized ω-transaminases on controlled porosity glass metal-ion affinity carrier

  2018cites this paper
• Cross-Linked Enzyme Aggregates for Applications in Aqueous and Nonaqueous Media.

  2017cites this paper
• Organic–inorganic hybrid nanoflowers: A novel host platform for immobilizing biomolecules

  2017cites this paper
• Effect of feather meal as proteic feeder on combi-CLEAs preparation for grape juice clarification

  2017cites this paper
• Synthesis of Benzyl Acetate Catalyzed by Lipase Immobilized in Nontoxic Chitosan-Polyphosphate Beads

  2017cites this paper
• Industrial and Therapeutic Enzymes: Penicillin Acylase

  2017cites this paper
• Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts.

  2017cites this paper
• Bioremediation: A Green, Sustainable and Eco‐Friendly Technique for the Remediation of Pollutants

  2017cites this paper
• Diffusion effects of bovine serum albumin on cross-linked aggregates of catalase

  2016cites this paper
• Cross-linked enzyme aggregates (CLEAs) of Pencilluim notatum lipase enzyme with improved activity, stability and reusability characteristics.

  2016cites this paper
• Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities.

  2016cites this paper
• APERFEIÇOAMENTO DAS PROPRIEDADES CATALÍTICAS DE LECITASE IMOBILIZADA UTILIZANDO POLÍMEROS IÔNICOS (um espaço) (um espaço)

  2015cites this paper
• Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges

  2015cites this paper
• Amphiphilic polymer conetworks with defined nanostructure and tailored swelling behavior for exploring the activation of an entrapped lipase in organic solvents

  2015cites this paper
• Xylanase Immobilized on Novel Multifunctional Hyperbranched Polyglycerol-Grafted Magnetic Nanoparticles: An Efficient and Robust Biocatalyst.

  2015cites this paper
• Easily handling penicillin G acylase magnetic cross-linked enzymes aggregates: Catalytic and morphological studies

  2014cites this paper
• Preparation of Cross-Linked Enzyme Aggregates of Trehalose Synthase via Co-aggregation with Polyethyleneimine

  2014cites this paper
• Hybrid Magnetic Cross-Linked Enzyme Aggregates of Phenylalanine Ammonia Lyase from Rhodotorula glutinis

  2014cites this paper
• Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers.

  2014cites this paper
• Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

  2014cites this paper
• Carbon Nanotubes as Activating Tyrosinase Supports for the Selective Synthesis of Catechols

  2014cites this paper
• Preparation of cross-linked enzyme aggregates in water-in-oil emulsion: Application to trehalose synthase

  2014cites this paper
• Versatile and Efficient Immobilization of 2-Deoxyribose-5-phosphate Aldolase (DERA) on Multiwalled Carbon Nanotubes

  2014cites this paper
• Modulation of the Microenvironment Surrounding the Active Site of Penicillin G Acylase Immobilized on Acrylic Carriers Improves the Enzymatic Synthesis of Cephalosporins

  2013cites this paper
• Potential Applications of Carbohydrases Immobilization in the Food Industry

  2013cites this paper
• A Simple Technique of Preparing Stable CLEAs of Phenylalanine Ammonia Lyase Using Co-aggregation with Starch and Bovine Serum Albumin

  2013cites this paper
• New opportunities for immobilization of enzymes.

  2013cites this paper
• Effect of inactivation and reactivation conditions on activity recovery of enzyme catalysts

  2013cites this paper
• Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs)

  2013cites this paper
• Polymer Directed Protein Assemblies

  2013cites this paper
• Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties.

  2013cites this paper
• Novel magnetic cross-linked enzyme aggregates (magnetic CLEAs) of alpha amylase.

  2012cites this paper
• Preparation of cross-linked enzyme aggregates of L-aminoacylase via co-aggregation with polyethyleneimine

  2012cites this paper
• Reactivation of penicillin acylase biocatalysts: Effect of the intensity of enzyme–support attachment and enzyme load

  2012cites this paper
• Immobilization of Candida antarctica A and Thermomyces lanuginosus lipases on cotton terry cloth fibrils using polyethyleneimine.

  2012cites this paper
• Chemical amination of lipase B from Candida antarctica is an efficient solution for the preparation of crosslinked enzyme aggregates

  2012cites this paper
• Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking

  2012cites this paper
• University of Groningen Properties of Immobilized Candida antarctica Lipase B on Highly Macroporous Copolymer Handayani, Nurrahmi; Miletic, Nemanja; Loos, Katja; Achmad, Sadijah; Wahyuningrum, Deana

  2011cites this paper
• Preparation of cross-linked enzyme aggregates of Aspergillus melleus aminoacylase for enantioselective synthesis of unnatural amino acids

  2011influential citation
• Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase

  2011cites this paper
• Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

  2011cites this paper
• Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

  2011cites this paper
• Immobilized β-galactosidase onto magnetic particles coated with polyaniline: Support characterization and galactooligosaccharides production

  2011cites this paper
• Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization.

  2011cites this paper
• Properties of Immobilized Candida antarctica Lipase B on Highly Macroporous Copolymer

  2011cites this paper
• Enzyme stabilization via cross-linked enzyme aggregates.

  2011cites this paper
• Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process

  2010cites this paper
• Proteases as Powerful Catalysts for Organic Synthesis

  2010cites this paper
• Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support.

  2010cites this paper
• Post-immobilization of modified macromolecular reagents using assembled penicillin acylase for microenvironmental regulation of nanopores and enhancement of enzyme stability

  2010cites this paper
• Biotransformation of amides to acids using a co-cross-linked enzyme aggregate of Rhodococcus erythropolis amidase.

  2010cites this paper
• Preparation of cross-linked aggregates of aminoacylase from Aspergillus melleus by using bovine serum albumin as an inert additive.

  2010cites this paper
• Use of enzymes in the production of semi-synthetic penicillins and cephalosporins: drawbacks and perspectives.

  2010cites this paper
• Penicillin G splitting in a flow-through electro-membrane reactor with the membrane-bound enzyme

  2009cites this paper
• Quantitative characteristic of the catalytic properties and microstructure of cross-linked enzyme aggregates of penicillin acylase

  2009cites this paper
• Lipase immobilization on modified zirconia nanoparticles: Studies on the effects of modifiers

  2009cites this paper
• Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

  2009cites this paper
• Enhancement of microwave-assisted covalent immobilization of penicillin acylase using macromolecular crowding and glycine quenching.

  2009cites this paper
• Formation of cross-linked chloroperoxidase aggregates in the pores of mesocellular foams: characterization by SANS and catalytic properties.

  2009cites this paper
• Effect of the degree of cross-linking on the properties of different CLEAs of penicillin acylase

  2009cites this paper
• Penicillin acylase immobilization depending on macromolecular crowding and catalysis in aqueous–organic medium

  2009cites this paper
• Synthesis of Cephalexin in Aqueous Medium with Carrier-bound and Carrier-free Penicillin Acylase Biocatalysts

  2009cites this paper
• Simple strategy of reactivation of a partially inactivated penicillin g acylase biocatalyst in organic solvent and its impact on the synthesis of β‐lactam antibiotics

  2009cites this paper
• Enzyme immobilization on amphiphilic polymer particles having grafted polyionic polymer chains

  2009cites this paper
• Thermodynamic and kinetic stability of penicillin acylase from Escherichia coli.

  2008cites this paper
• Study Cases of Enzymatic Processes

  2008cites this paper
• Immobilization of Biological Catalysts

  2008cites this paper
• Carrier-bound and carrier-free penicillin acylase biocatalysts for the thermodynamically controlled synthesis of β-lactam compounds in organic medium

  2008cites this paper