Antibody-mediated target cell lysis by nonimmune cells: the use of anti-immunoglobulin to distinguish effector cell population.

The role of a goat IgG anti-rabbit immunoglobulin on antibody-mediated target cell lysis by nonimmune cells was investigated. Using a homologous system of rabbit effector cells and rabbit anti-fowl erythrocyte serum, two populations of cells effective in the lysis of antibody-coated target cells could be differentiated by anti-immunoglobulin. In the presence of the anti-immunoglobulin, the cytotoxicity caused by phagocytic cells was suppressed. The IgG or (Fab′)2 fraction of the anti-immunoglobulin enhanced target cell destruction initiated by purified lymph node and spleen lymphocytes and to a lesser extent that initiated by purified thymus cells. Monovalent (Fab) anti-immunoglobulin decreased the cytotoxic activity of all effector cells. The mechanism of enhancement appeared to be due to cross-linking between effector lymphocytes and antibody-coated target cells by the divalent anti-immunoglobulin.

• Publication year

  1974

• Venue

  Journal of Immunology

• Publication date

  1974-02-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.112.2.792PMID 4544363
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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