DNA-induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase.

No abstract is available for this paper.

• Publication year

  2006

• Venue

  Molecules and Cells

• Publication date

  2006-01-20

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/J.MOLCEL.2005.11.027PMID 16427007
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mechanochemistry of t7 DNA helicase.

  2005influential reference
• Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines

  2005cited by this paper
• The arginine finger of bacteriophage T7 gene 4 helicase: role in energy coupling.

  2004cited by this paper
• Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues.

  2003cited by this paper
• Interaction of adjacent primase domains within the hexameric gene 4 helicase-primase of bacteriophage T7

  2002cited by this paper
• Kinetic Pathway of dTTP Hydrolysis by Hexameric T7 Helicase-Primase in the Absence of DNA*

  2002influential reference
• Site-directed mutagenesis reveals roles for conserved amino acid residues in the hexameric DNA helicase DnaB from Bacillus stearothermophilus.

  2002cited by this paper
• T7 DNA helicase: a molecular motor that processively and unidirectionally translocates along single-stranded DNA.

  2002cited by this paper
• The Nucleotide Binding Site of the Helicase / Primase of Bacteriophage T 7

  2001cited by this paper
• Essential Lysine Residues in the RNA Polymerase Domain of the Gene 4 Primase-Helicase of Bacteriophage T7*

  2001cited by this paper
• A Domain of the Gene 4 HelicaseIPrimase of Bacteriophage T7 Required for the Formation of an Active Hexamer*

  2001cited by this paper
• Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides.

  2000influential reference
• Structure and function of hexameric helicases.

  2000cited by this paper
• DNA binding in the central channel of bacteriophage T7 helicase-primase is a multistep process. Nucleotide hydrolysis is not required.

  2000cited by this paper
• A ring‐opening mechanism for DNA binding in the central channel of the T7 helicase–primase protein

  2000cited by this paper
• Toxic mutations in the recA gene of E. coli prevent proper chromosome segregation.

  1999cited by this paper
• Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7.

  1999cited by this paper
• On the in vivo function of the RecA ATPase.

  1999cited by this paper
• The dTTPase mechanism of T7 DNA helicase resembles the binding change mechanism of the F1-ATPase.

  1997influential reference
• Biochemical Analysis of Mutant T7 Primase/Helicase Proteins Defective in DNA Binding, Nucleotide Hydrolysis, and the Coupling of Hydrolysis with DNA Unwinding*

  1996influential reference
• Catalytic and EPR studies of the beta E204Q mutant of the chloroplast F1-ATPase from Chlamydomonas reinhardtii.

  1996cited by this paper
• An attempt to convert noncatalytic nucleotide binding site of F1-ATPase to the catalytic site: hydrolysis of tethered ATP by mutated alpha subunits in the enzyme.

  1996cited by this paper
• DNA is bound within the central hole to one or two of the six subunits of the T7 DNA helicase

  1996influential reference
• Mechanisms of helicase-catalyzed DNA unwinding.

  1996cited by this paper
• A common topology of proteins catalyzing ATP‐triggered reactions

  1995cited by this paper
• Nucleotide and DNA-induced Conformational Changes in the Bacteriophage T7 Gene 4 Protein (*)

  1995cited by this paper
• Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases.

  1995cited by this paper
• The K318A mutant of bacteriophage T7 DNA primase-helicase protein is deficient in helicase but not primase activity and inhibits primase-helicase protein wild-type activities by heterooligomer formation.

  1994cited by this paper
• Structure at 2.8 Â resolution of F1-ATPase from bovine heart mitochondria

  1994cited by this paper
• Conserved Glu-181 and Arg-182 residues of Escherichia coli H(+)-ATPase (ATP synthase) beta subunit are essential for catalysis: properties of 33 mutants between beta Glu-161 and beta Lys-201 residues.

  1994cited by this paper
• Interactions of bacteriophage T7 DNA primase/helicase protein with single-stranded and double-stranded DNAs.

  1993cited by this paper
• The nucleotide binding site of the helicase/primase of bacteriophage T7. Interaction of mutant and wild-type proteins.

  1993cited by this paper
• The binding change mechanism for ATP synthase--some probabilities and possibilities.

  1993influential reference
• Roles of bacteriophage T7 gene 4 proteins in providing primase and helicase functions in vivo.

  1992cited by this paper
• Large scale purification and biochemical characterization of T7 primase/helicase proteins. Evidence for homodimer and heterodimer formation.

  1992influential reference
• Further examination of seventeen mutations in Escherichia coli F1-ATPase beta-subunit.

  1992cited by this paper
• Structure of the recA protein–ADP complex

  1992influential reference
• DNA helicases.

  1990cited by this paper
• A 7-kDa region of the bacteriophage T7 gene 4 protein is required for primase but not for helicase activity.

  1988cited by this paper
• Nucleotide-dependent binding of the gene 4 protein of bacteriophage T7 to single-stranded DNA.

  1985cited by this paper
• DNA-dependent nucleoside 5'-triphosphatase activity of the gene 4 protein of bacteriophage T7.

  1983cited by this paper
• Template recognition sequence for RNA primer synthesis by gene 4 protein of bacteriophage T7.

  1981cited by this paper
• Large Scale Purification and Biochemical Characterization of T 7 Primase / Helicase Proteins

  year unknowninfluential reference

• DNA translocation by the CMG helicase: the helical inchworm model.

  2026cites this paper
• Molecular Mechanism of ATP Hydrolysis Catalyzed by p97: A QM/MM Study

  2025cites this paper
• DnaB and DciA: mechanisms of helicase loading and translocation on ssDNA

  2025cites this paper
• Single-molecule fluorescence reveals the DNA unwinding mechanism of mitochondrial helicase TWINKLE and its interplay with single-stranded DNA-binding proteins

  2025cites this paper
• Single-molecule FRET probes allosteric effects on protein-translocating pore loops of a AAA+ machine

  2023cites this paper
• Structural and dynamic basis of DNA capture and translocation by mitochondrial Twinkle helicase

  2022influential citation
• DNA Polymerase-Parental DNA Interaction Is Essential for Helicase-Polymerase Coupling during Bacteriophage T7 DNA Replication

  2022influential citation
• Residues located in the primase domain of the bacteriophage T7 primase-helicase are essential for loading the hexameric complex onto DNA

  2022cites this paper
• Computationally exploring the mechanism of bacteriophage T7 gp4 helicase translocating along ssDNA

  2022cites this paper
• Assembling bacteriophage T7 leading-strand replisome for structural investigation.

  2022cites this paper
• Mechanisms of hexameric helicases

  2021cites this paper
• Replication | DNA Helicases: Hexameric Enzyme Action

  2021cites this paper
• DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication

  2021cites this paper
• Non-tight and tight chemomechanical couplings of biomolecular motors under hindering loads.

  2020cites this paper
• The Cryo-EM structure of AAV2 Rep68 in complex with ssDNA reveals a malleable AAA+ machine that can switch between oligomeric states

  2020cites this paper
• Structural studies reveal a ring-shaped architecture of deep-sea vent phage NrS-1 polymerase

  2020cites this paper
• On DNA Motions under Action of Enzymes of Different Types. II

  2019cites this paper
• Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form III)

  2019cites this paper
• Structures and operating principles of the replisome

  2019cites this paper
• Hexameric helicase G40P unwinds DNA in single base pair steps

  2018influential citation
• On DNA Motions under Action of Enzymes of Different Types. I

  2018cites this paper
• Hybrid Methods Reveal Multiple Flexibly Linked DNA Polymerases within the Bacteriophage T7 Replisome.

  2017influential citation
• Cryo-EM structure of the replisome reveals multiple interactions coordinating DNA synthesis

  2017cites this paper
• Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

  2016cites this paper
• The Replication System of Bacteriophage T7.

  2016cites this paper
• Dynamics of monomeric and hexameric helicases.

  2016cites this paper
• It seems like only yesterday.

  2015cites this paper
• Mutant poisoning demonstrates a nonsequential mechanism for digestion of double-stranded DNA by λ exonuclease trimers.

  2015cites this paper
• Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other

  2015cites this paper
• Mechanism of subunit coordination of an AAA+ hexameric molecular nanomachine.

  2015cites this paper
• Helicase Mechanism of Action

  2014cites this paper
• Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase

  2014cites this paper
• Coordination and control inside simple biomolecular machines.

  2014cites this paper
• Helicase Purification for DNA Sequencing

  2014cites this paper
• Mechanical operation and intersubunit coordination of ring-shaped molecular motors: insights from single-molecule studies.

  2014cites this paper
• Single molecule fluorescence studies of replicative helicases

  2014cites this paper
• Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea

  2014influential citation
• Discrete interactions between bacteriophage T7 primase-helicase and DNA polymerase drive the formation of a priming complex containing two copies of DNA polymerase.

  2013cites this paper
• The minichromosome maintenance replicative helicase.

  2013cites this paper
• Helicase Unwinding at the Replication Fork

  2013cites this paper
• Dissecting the Operating Mechanism of a Biological Motor One Molecule at a Time

  2013cites this paper
• Mechanism of RNA packaging motor.

  2012cites this paper
• The hexameric helicase DnaB adopts a nonplanar conformation during translocation.

  2012cites this paper
• Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structures

  2012cites this paper
• Oligomeric Properties of Adeno-Associated Virus Rep68 Reflect Its Multifunctionality

  2012cites this paper
• High degree of coordination and division of labor among subunits in a homomeric ring ATPase.

  2012cites this paper
• MCM structure and mechanics: what we have learned from archaeal MCM.

  2012cites this paper
• Heterohexamer of 56- and 63-kDa Gene 4 Helicase-Primase of Bacteriophage T7 in DNA Replication*

  2012cites this paper
• Dynamic coupling between the motors of DNA replication: hexameric helicase, DNA polymerase, and primase.

  2011cites this paper
• AMYLOID REMODELING BY Hsp 104

  2011cites this paper
• Coupling dTTP Hydrolysis with DNA Unwinding by the DNA Helicase of Bacteriophage T7*

  2011cites this paper
• The nuts and bolts of ring-translocase structure and mechanism.

  2011cites this paper
• The Glutamate Switch of Bacteriophage T7 DNA Helicase

  2011cites this paper
• Crystal structures of λ exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity

  2011cites this paper
• AMYLOID REMODELING BY Hsp104

  2011cites this paper
• Investigating high-affinity non-covalent protein-ligand interaction via variants of streptavidin

  2011cites this paper
• ATP-Induced Helicase Slippage Reveals Highly Coordinated Subunits

  2011cites this paper
• Viral DNA Packaging: One Step at a Time

  2010cites this paper
• FtsK DNA Translocase: The Fast Motor That Knows Where It's Going

  2010cites this paper
• Micro-manipulation de l'ADN Vers une visualisation directe par microscopie de fluorescence

  2010cites this paper
• Molecular Basis for Recognition of Nucleoside Triphosphate by Gene 4 Helicase of Bacteriophage T7*

  2010cites this paper
• Residues in the central β-hairpin of the DNA helicase of bacteriophage T7 are important in DNA unwinding

  2010cites this paper
• Mechanochemistry of a viral DNA packaging motor.

  2010cites this paper
• Dynamic Effects of Cofactors and DNA on the Oligomeric State of Human Mitochondrial DNA Helicase*

  2010cites this paper
• Clinical phenotype of autosomal dominant progressive external ophthalmoplegia in a family with a novel mutation in the C10orf2 gene

  2010cites this paper
• Foot-and-Mouth Disease Virus 2C Is a Hexameric AAA+ Protein with a Coordinated ATP Hydrolysis Mechanism

  2010cites this paper
• Separating speed and ability to displace roadblocks during DNA translocation by FtsK

  2010cites this paper
• ATP Utilization by the DEAD-Box Protein DED1P

  2010cites this paper
• Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins.

  2010cites this paper
• Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase

  2009cites this paper
• Inter-Subunit Coordination in a Homomeric Ring-ATPase

  2009cites this paper
• An in Trans Interaction at the Interface of the Helicase and Primase Domains of the Hexameric Gene 4 Protein of Bacteriophage T7 Modulates Their Activities*

  2009cites this paper
• Chromatin remodeling finds its place in the DNA double-strand break response

  2009cites this paper
• A structure-based sequential mechano-chemical model of hexameric helicases

  2009cites this paper
• Running in reverse: the structural basis for translocation polarity in hexameric helicases.

  2009cites this paper
• Coupling ATP utilization to protein remodeling by ClpB, a hexameric AAA+ protein

  2009cites this paper
• The Mcm Complex: Unwinding the Mechanism of a Replicative Helicase

  2009cites this paper
• Motors, switches, and contacts in the replisome.

  2009cites this paper
• Promiscuous Usage of Nucleotides by the DNA Helicase of Bacteriophage T7

  2009cites this paper
• Physical aspects of the structure and function of helicases as rotary molecular motors

  2009cites this paper
• Modelling of a DNA packaging motor

  2009cites this paper
• On helicases and other motor proteins.

  2008cites this paper
• Modulation of mitochondrial DNA replication and recombination in mammalian tissues and cultured cells

  2008cites this paper
• Coupling and dynamics of subunits in the hexameric AAA+ chaperone ClpB.

  2008cites this paper
• Structural Basis of Mechanochemical Coupling in a Hexameric Molecular Motor*

  2008cites this paper
• Viral Helicases

  2008cites this paper
• Coupling of DNA unwinding to nucleotide hydrolysis in a ring‐shaped helicase

  2008cites this paper
• Systematic Study of the Functions for the Residues around the Nucleotide Pocket in Simian Virus 40 AAA+ Hexameric Helicase

  2008cites this paper
• Physiological and Biochemical Defects in Carboxyl-terminal Mutants of Mitochondrial DNA Helicase*

  2008cites this paper
• RNA packaging motor: From structure to quantum mechanical modelling and sequential-stochastic mechanism

  2008cites this paper
• Communication between subunits critical to DNA binding by hexameric helicase of bacteriophage T7

  2008cites this paper
• Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus NS3 helicase: magnesium provides a bridge for ATP to fuel unwinding.

  2007cites this paper
• Model for RuvAB-mediated branch migration of Holliday junctions.

  2007cites this paper
• Building KiMoSin : design requirements for kinetic interfaces in protein education

  2007cites this paper
• ATPase site architecture and helicase mechanism of an archaeal MCM.

  2007cites this paper
• Expression of catalytic mutants of the mtDNA helicase Twinkle and polymerase POLG causes distinct replication stalling phenotypes

  2007cites this paper
• Structure and mechanism of helicases and nucleic acid translocases.

  2007cites this paper
• Twinkle helicase (PEO1) gene mutation causes mitochondrial DNA depletion

  2007cites this paper
• On translocation mechanism of ring-shaped helicase along single-stranded DNA.

  2007cites this paper
• SURVEY AND SUMMARY Mechanisms of a ring shaped helicase

  2006cites this paper