Site‐directed mutagenesis of a fatty acid elongase ELO‐like condensing enzyme

The condensation step of fatty acid elongation is the addition of a C2 unit from malonyl‐CoA to an acyl primer catalyzed by one of two families of enzymes, the 3‐ketoacyl‐CoA synthases and the ELO‐like condensing enzymes. 3‐Ketoacyl‐CoA synthases use a Claisen‐like reaction mechanism while the mechanism of the ELO‐catalyzed condensation reaction is unknown. We have used site‐directed mutagenesis of Dictyostelium discoideum EloA to identify residues important to catalytic activity and/or structure. Mutation of highly conserved polar residues to alanine resulted in an inactive enzyme strongly suggesting that these residues play a role in the condensation reaction.

• Publication year

  2013

• Venue

  FEBS Letters

• Publication date

  2013-11-29

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.febslet.2013.10.011PMID 24157363
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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