The structure of human thioredoxin reductase 1 provides insights into C-terminal rearrangements during catalysis.

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Journal of Molecular Biology

• Publication date

  2007-06-29

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/J.JMB.2007.04.044PMID 17512005
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Truncated mutants of human thioredoxin reductase 1 do not exhibit glutathione reductase activity

  2006cited by this paper
• Mutational Studies Confirm the Catalytic Triad in the Human Selenoenzyme Thioredoxin Reductase Predicted by Molecular Modeling

  2006cited by this paper
• On the potential of thioredoxin reductase inhibitors for cancer therapy.

  2006cited by this paper
• Studies of an active site mutant of the selenoprotein thioredoxin reductase: the Ser-Cys-Cys-Ser motif of the insect orthologue is not sufficient to replace the Cys-Sec dyad in the mammalian enzyme.

  2006cited by this paper
• Thioredoxin and Its Related Molecules: Update 2005

  2005cited by this paper
• Inhibition of thioredoxin reductase but not of glutathione reductase by the major classes of alkylating and platinum-containing anticancer compounds.

  2005cited by this paper
• The Functional Role of Selenocysteine (Sec) in the Catalysis Mechanism of Large Thioredoxin Reductases: Proposition of a Swapping Catalytic Triad Including a Sec‐His‐Glu State

  2005cited by this paper
• Crystal structures of oxidized and reduced mitochondrial thioredoxin reductase provide molecular details of the reaction mechanism.

  2005cited by this paper
• Evidence for intriguingly complex transcription of human thioredoxin reductase 1.

  2004cited by this paper
• The thioredoxin system—From science to clinic

  2004cited by this paper
• The Mammalian Testis-Specific Thioredoxin System

  2004cited by this paper
• Flavoprotein disulfide reductases: advances in chemistry and function.

  2004cited by this paper
• The Mechanism of High Mr Thioredoxin Reductase from Drosophila melanogaster*

  2003cited by this paper
• Glutathione reductase of the malarial parasite Plasmodium falciparum: crystal structure and inhibitor development.

  2003cited by this paper
• Rapid Induction of Cell Death by Selenium-compromised Thioredoxin Reductase 1 but Not by the Fully Active Enzyme Containing Selenocysteine*

  2003cited by this paper
• Non‐animal origin of animal thioredoxin reductases: Implications for selenocysteine evolution and evolution of protein function through carboxy‐terminal extensions

  2003cited by this paper
• Selenoprotein oxidoreductase with specificity for thioredoxin and glutathione systems

  2001cited by this paper
• Roles of N-terminal active cysteines and C-terminal cysteine-selenocysteine in the catalytic mechanism of mammalian thioredoxin reductase.

  2001cited by this paper
• Overexpression of wild type and SeCys/Cys mutant of human thioredoxin reductase in E. coli: the role of selenocysteine in the catalytic activity.

  2001cited by this paper
• Heterogeneity within Animal Thioredoxin Reductases

  2001cited by this paper
• Three-dimensional structure of a mammalian thioredoxin reductase: Implications for mechanism and evolution of a selenocysteine-dependent enzyme

  2001cited by this paper
• Thioredoxin-thioredoxin reductase--a system that has come of age.

  2000cited by this paper
• Mammalian thioredoxin reductase: oxidation of the C-terminal cysteine/selenocysteine active site forms a thioselenide, and replacement of selenium with sulfur markedly reduces catalytic activity.

  2000cited by this paper
• Thioredoxin reductase.

  2000cited by this paper
• Physiological functions of thioredoxin and thioredoxin reductase.

  2000cited by this paper
• Structure and mechanism of mammalian thioredoxin reductase: the active site is a redox-active selenolthiol/selenenylsulfide formed from the conserved cysteine-selenocysteine sequence.

  2000cited by this paper
• Essential Role of Selenium in the Catalytic Activities of Mammalian Thioredoxin Reductase Revealed by Characterization of Recombinant Enzymes with Selenocysteine Mutations*

  2000cited by this paper
• Interaction of peroxynitrite with selenoproteins and glutathione peroxidase mimics.

  2000cited by this paper
• Production of functional human selenocysteine-containing KDRF/thioredoxin reductase in E. coli.

  2000cited by this paper
• High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes.

  1999cited by this paper
• Cloning, sequencing and functional expression of a novel human thioredoxin reductase

  1999cited by this paper
• Further additions to MolScript version 1.4, including reading and contouring of electron-density maps.

  1999cited by this paper
• Human mitochondrial thioredoxin reductase cDNA cloning, expression and genomic organization.

  1999cited by this paper
• Redox Regulation of Cell Signaling by Selenocysteine in Mammalian Thioredoxin Reductases*

  1999cited by this paper
• Human thioredoxin reductase from HeLa cells: selective alkylation of selenocysteine in the protein inhibits enzyme activity and reduction with NADPH influences affinity to heparin.

  1998cited by this paper
• Human Placenta Thioredoxin Reductase

  1998cited by this paper
• A hypothesis on the catalytic mechanism of the selenoenzyme thioredoxin reductase.

  1998cited by this paper
• Crystallography & NMR system: A new software suite for macromolecular structure determination.

  1998cited by this paper
• The mechanism of thioredoxin reductase from human placenta is similar to the mechanisms of lipoamide dehydrogenase and glutathione reductase and is distinct from the mechanism of thioredoxin reductase from Escherichia coli.

  1997cited by this paper
• Raster3D: photorealistic molecular graphics.

  1997cited by this paper
• Thioredoxin reductase activity is decreased by selenium deficiency.

  1997cited by this paper
• Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene.

  1996cited by this paper
• Kinetics and Crystallographic Analysis of Human Glutathione Reductase in Complex with a Xanthene Inhibitor (*)

  1996cited by this paper
• Cloning and sequencing of a human thioredoxin reductase

  1995cited by this paper
• Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants

  1993cited by this paper
• PROCHECK: a program to check the stereochemical quality of protein structures

  1993cited by this paper
• Chemistry and Biochemistry of Flavoenzymes: Volume I

  1991cited by this paper
• Improved methods for building protein models in electron density maps and the location of errors in these models.

  1991cited by this paper
• A program to produce both detailed and schematic plots of protein structures

  1991cited by this paper
• A crystallographic study of the glutathione binding site of glutathione reductase at 0.3-nm resolution.

  1989cited by this paper
• Refined structure of glutathione reductase at 1.54 A resolution.

  1987cited by this paper

• Dual-Proximity Labeling Strategy Identifies TrxR1 as a Cuproprotein that Regulates Apoptosis via Caspase-3 S-Nitrosylation.

  2026cites this paper
• Cysteine Alkylation in Enzymes and Transcription Factors: A Therapeutic Strategy for Cancer

  2025cites this paper
• Apoptosis-inducing factor (AIF), dihydrolipoamide dehydrogenase (DLD), glutathione disulfide reductase (GSR), and thioredoxin reductase (TrxR) in cancer and neurological disorders: Structural insights, redox regulation, and therapeutic potential.

  2025cites this paper
• From selenium to sulfur: predictive modeling unveils conformational and bonding changes in selenoproteins.

  2025cites this paper
• Unconventional chalcogen-containing azolylidene metal complexes as potential anticancer therapeutics

  2025cites this paper
• Cysteine-based biomolecules regulate cellular copper- and redox-homeostasis

  2025cites this paper
• Antiproliferative effects, mechanism of action and tumor reduction studies in a lung cancer xenograft mouse model of an organometallic gold(i) alkynyl complex

  2025cites this paper
• Organometallic and coordination gold(I)-azole drugs compounds: Synthesis, characterization, antitumor evaluation, and interactions with biomolecules.

  2025cites this paper
• Mutational analysis of TXNRD1 reveals the essential role of Trp114 in TRP14 reduction and identifies key determinants of enzymatic activity and thermostability.

  2025cites this paper
• Targeting Apicomplexan Parasites: Structural and Functional Characterization of Cryptosporidium Thioredoxin Reductase as a Novel Drug Target.

  2025cites this paper
• S-Se oxidative addition to auranofin derivatives: a DFT study.

  2024cites this paper
• The C-terminal selenenylsulfide of extracellular/non-reduced thioredoxin reductase endows this protein with selectivity to small-molecule electrophilic reagents under oxidative conditions

  2024cites this paper
• Building on a theme: The redox hierarchy of pyridine nucleotide-disulfide oxidoreductases.

  2024cites this paper
• Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif

  2024influential citation
• Hypothiocyanous acid reductase is critical for host colonization and infection by Streptococcus pneumoniae

  2024cites this paper
• Simulating the Motion Underlying the Mechanism of Thioredoxin Reductase

  2024cites this paper
• Investigation on X-ray diffraction, IR, UV-visible, Fluorescence, Thermogravimetric, DFT studies, and Molecular docking approach of a new C6H17.16Cl3CuN3O0.58 complex

  2024cites this paper
• Exploring the Anticancer Activity of Tamoxifen-Based Metal Complexes Targeting Mitochondria

  2023cites this paper
• Enzymatic Redox Properties and Cytotoxicity of Irreversible Nitroaromatic Thioredoxin Reductase Inhibitors in Mammalian Cells

  2023cites this paper
• Selenium—More than Just a Fortuitous Sulfur Substitute in Redox Biology

  2023cites this paper
• Cytotoxic auranofin analogues bearing phosphine, arsine and stibine ligands: A study on the possible role of the ligand on the biological activity.

  2023cites this paper
• Exploration of 3,4-unsubstituted coumarins as thioredoxin reductase 1 inhibitors for cancer therapy.

  2023cites this paper
• Thioredoxin Reductase and Organometallic Complexes: A Pivotal System to Tackle Multidrug Resistant Tumors?

  2023cites this paper
• Novel starting points for fragment-based drug design against mycobacterial thioredoxin reductase identified using crystallographic fragment screening

  2023cites this paper
• Gold(III) heteroleptic complexes with SNS-thiosemicarbazonate ligands as cytotoxic agents: experimental and computational insights into the mechanism of action

  2022cites this paper
• Adenanthin Is an Efficient Inhibitor of Peroxiredoxins from Pathogens, Inhibits Bacterial Growth, and Potentiates Antibiotic Activities.

  2022cites this paper
• Deciphering the Path of S-nitrosation of Human Thioredoxin: Evidence of an Internal NO Transfer and Implication for the Cellular Responses to NO

  2022cites this paper
• Conformational change due to replacement of disulfide with selenosulfide and diselenide in dipeptide vicinal cysteine loop

  2022cites this paper
• Analysis of Pleurotin binding to human thioredoxin reductase using docking and molecular dynamics simulation

  2022influential citation
• Thioredoxin reductase selenoproteins from different organisms as potential drug targets for treatment of human diseases.

  2022cites this paper
• S-Denitrosylation: A Crosstalk between Glutathione and Redoxin Systems

  2022cites this paper
• Au4 cluster inhibits human thioredoxin reductase activity via specifically binding of Au to Cys189

  2022cites this paper
• Characterization of a small molecule inhibitor of disulfide reductases that induces oxidative stress and lethality in lung cancer cells.

  2022cites this paper
• Biochemical and structural characterizations of thioredoxin reductase selenoproteins of the parasitic filarial nematodes Brugia malayi and Onchocerca volvulus

  2022cites this paper
• Selenocysteine of thioredoxin reductase as the primary target for the antitumor metallodrugs: A computational point of view

  2022cites this paper
• A novel small molecule that induces cytotoxicity in lung cancer cells inhibits disulfide reductases GSR and TXNRD1

  2021cites this paper
• Nitroisatin dithiocarbazate: Synthesis, structural characterization, DFT, and docking studies

  2021cites this paper
• Thioredoxin reductase 1 inhibitor shikonin promotes cell necroptosis via SecTRAPs generation and oxygen-coupled redox cycling.

  2021cites this paper
• Role of the Enzymatic Environment in the Reactivity of the AuIII-C^N^C Anticancer Complexes.

  2021cites this paper
• [Potential Anticancer Activity of Auranofin].

  2021cites this paper
• Probing the Surface of a Parasite Drug Target Thioredoxin Glutathione Reductase Using Small Molecule Fragments.

  2021cites this paper
• Selective, Modular Probes for Thioredoxins Enabled by Rational Tuning of a Unique Disulfide Structure Motif.

  2021cites this paper
• Characterization of lead compounds targeting the selenoprotein thioredoxin glutathione reductase for treatment of schistosomiasis.

  2020cites this paper
• Density functional theory study of gold(III)-dithiocarbamate complexes with characteristic anticancer potentials.

  2020cites this paper
• Effects of Mammalian Thioredoxin Reductase Inhibitors.

  2020cites this paper
• Studies of the thioredoxin system in redox signaling and oxidative stress

  2020cites this paper
• Perspectives of TrxR1-based cancer therapies

  2020cites this paper
• Novel electrophilic amides amenable by the Ugi reaction perturb thioredoxin system via thioredoxin reductase 1 (TrxR1) inhibition: Identification of DVD-445 as a new lead compound for anticancer therapy.

  2019cites this paper
• Mitochondria-Targeting Anticancer Metal Complexes.

  2019cites this paper
• A gold(i) biscarbene complex with improved activity as a TrxR inhibitor and cytotoxic drug: comparative studies with different gold metallodrugs.

  2019cites this paper
• Methylmercury's chemistry: From the environment to the mammalian brain.

  2019cites this paper
• Proteasome vs Thioredoxin reductase competition as possible biological targets in antitumor mixed thiolate- dithiocarbamate gold(III) complexes

  2019cites this paper
• Virtual screening‐guided discovery of thioredoxin reductase inhibitors

  2019cites this paper
• Targeting the Thioredoxin System as a Strategy for Cancer Therapy.

  2019cites this paper
• DFT Study of the Structural and Electronic Properties of Selected Organogold(III) Compounds with Characteristic Anticancer Activity

  2019cites this paper
• Structural and biochemical studies on three Aspergillus fumigatus proteins that present as targets for novel antifungal drugs

  2018cites this paper
• Proteasome versus Thioredoxin Reductase Competition as Possible Biological Targets in Antitumor Mixed Thiolate-Dithiocarbamate Gold(III) Complexes.

  2018cites this paper
• Interaction of High Molecular Weight Compounds with a, β-Unsaturated Carbonyl Moiety with Mammalian and Drosophila Melanogaster Thioredoxin Reductase

  2018cites this paper
• Cloning and functional characterization of thioredoxin genes from large yellow croaker Larimichthys crocea

  2018cites this paper
• Thioredoxin reductase inhibitors: a patent review

  2017cites this paper
• Preclinical anti-cancer activity and multiple mechanisms of action of a cationic silver complex bearing N-heterocyclic carbene ligands.

  2017cites this paper
• In vitro antitumor activity, metal uptake and reactivity with ascorbic acid and BSA of some gold(III) complexes with N,N'-ethylenediamine bidentate ester ligands.

  2017cites this paper
• The structure of Lactococcus lactis thioredoxin reductase reveals molecular features of photo-oxidative damage

  2017cites this paper
• The Enzymatic and Structural Basis for Inhibition of Echinococcus granulosus Thioredoxin Glutathione Reductase by Gold(I)

  2017cites this paper
• Targeting the Thioredoxin System for Cancer Therapy.

  2017cites this paper
• Light Sensitivity of Lactococcus lactis Thioredoxin Reductase

  2017cites this paper
• Organoselenium compounds as mimics of selenoproteins and thiol modifier agents.

  2017cites this paper
• Synthesis, SAR and biological evaluation of a novel series of 1-(2-chloroethyl)-1-nitroso-3-(2-(3-oxobenzoelenazol-2(3H)-yl)ethyl) urea: Organoselenium compounds for cancer therapy.

  2016cites this paper
• Ligand Exchange Reaction of Au(I) R-N-Heterocyclic Carbene Complexes with Cysteine.

  2016cites this paper
• Lactococcus lactis Thioredoxin Reductase Is Sensitive to Light Inactivation

  2016cites this paper
• Details in the catalytic mechanism of mammalian thioredoxin reductase 1 revealed using point mutations and juglone-coupled enzyme activities.

  2016cites this paper
• Fasciola gigantica thioredoxin glutathione reductase: Biochemical properties and structural modeling.

  2016cites this paper
• Cloning and characterization of selenoprotein thioredoxin reductase gene in Haematococcus pluvialis

  2016cites this paper
• Novel antitumor adamantane–azole gold(I) complexes as potential inhibitors of thioredoxin reductase

  2016cites this paper
• The role of electrostatics in TrxR electron transfer mechanism: A computational approach

  2016cites this paper
• Differentiating the Roles of Redox-Active Cysteine Residues in Plasmodium falciparum Thioredoxin Reductase by using a "Seleno Effect"

  2015cites this paper
• Lactococcus lactis thioredoxin reductase is sensitive to light inactivation.

  2015cites this paper
• TrxR1 as a Potent Regulator of the Nrf2-Keap1 Response System

  2015cites this paper
• Main cellular redox couples

  2015cites this paper
• Thioredoxin Cross-Linking by Nitrogen Mustard in Lung Epithelial Cells: Formation of Multimeric Thioredoxin/Thioredoxin Reductase Complexes and Inhibition of Disulfide Reduction.

  2015cites this paper
• Synthesis, characterization, and antitumor activity of unusual pseudo five coordinate gold(III) complexes: distinct cytotoxic mechanism or expensive ligand delivery systems?

  2014cites this paper
• Intricate aspects of the thioredoxin system in redox signaling

  2014cites this paper
• In-silico Screening of Gold-Based Compounds as Potential Non Competitive Inhibitors for Human Mitochondrial Thioredoxin Reductase

  2014cites this paper
• Antiglioma activity of GoPI-sugar, a novel gold(I)-phosphole inhibitor: chemical synthesis, mechanistic studies, and effectiveness in vivo.

  2014cites this paper
• The combined therapeutical effect of metal-based drugs and radiation therapy: the present status of research.

  2014cites this paper
• Gold organometallics with biological properties

  2014cites this paper
• Thioredoxin Reductase and its Inhibitors

  2014influential citation
• Acetaminophen Reactive Intermediates Target Hepatic Thioredoxin Reductase

  2014cites this paper
• Why Is Mammalian Thioredoxin Reductase 1 So Dependent upon the Use of Selenium?

  2014cites this paper
• A soluble bis-chelated gold(I) diphosphine compound with strong anticancer activity and low toxicity.

  2013cites this paper
• Antitumor properties of five-coordinate gold(III) complexes bearing substituted polypyridyl ligands.

  2013cites this paper
• Identification of Michael Acceptor-Centric Pharmacophores with Substituents That Yield Strong Thioredoxin Reductase Inhibitory Character Correlated to Antiproliferative Activity

  2013cites this paper
• A diterpenoid derivate compound targets selenocysteine of thioredoxin reductases and induces Bax/Bak-independent apoptosis.

  2013cites this paper
• Crystal structure of the Plasmodium falciparum thioredoxin reductase-thioredoxin complex.

  2013cites this paper
• Recent advances in the development of thioredoxin reductase inhibitors as anticancer agents.

  2012cites this paper
• Discovery and biochemical characterization of Plasmodium thioredoxin reductase inhibitors from an antimalarial set.

  2012cites this paper
• Inhibition of thioredoxin reductase by a novel series of bis-1,2-benzisoselenazol-3(2H)-ones: Organoselenium compounds for cancer therapy.

  2012cites this paper
• Reactions and structural characterization of gold(III) complexes with amino acids, peptides and proteins.

  2012cites this paper
• Gold(I) carbene complexes causing thioredoxin 1 and thioredoxin 2 oxidation as potential anticancer agents.

  2012cites this paper
• Thioredoxin reductase, an emerging target for anticancer metallodrugs

  2011cites this paper