Topoisomerase I‐mediated cleavage at unrepaired ribonucleotides generates DNA double‐strand breaks

No abstract is available for this paper.

• Publication year

  2017

• Venue

  EMBO Journal

• Publication date

  2017-02-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.15252/embj.201592426PMID 27932446PMCID PMC5286372
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Topoisomerase I‐mediated cleavage at unrepaired ribonucleotides generates DNA double‐strand breaks

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• Processing ribonucleotides incorporated during eukaryotic DNA replication

  2016cited by this paper
• The Balancing Act of Ribonucleotides in DNA.

  2016cited by this paper
• Roles of eukaryotic topoisomerases in transcription, replication and genomic stability

  2016cited by this paper
• Parallel analysis of ribonucleotide-dependent deletions produced by yeast Top1 in vitro and in vivo

  2016cited by this paper
• Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon

  2016cited by this paper
• Topoisomerase I Alone Is Sufficient to Produce Short DNA Deletions and Can Also Reverse Nicks at Ribonucleotide Sites*

  2015cited by this paper
• Stimulation of Chromosomal Rearrangements by Ribonucleotides

  2015cited by this paper
• Error‐free and mutagenic processing of topoisomerase 1‐provoked damage at genomic ribonucleotides

  2015influential reference
• Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity

  2015cited by this paper
• Evidence that processing of ribonucleotides in DNA by topoisomerase 1 is leading-strand specific

  2015cited by this paper
• Defective removal of ribonucleotides from DNA promotes systemic autoimmunity.

  2015influential reference
• Ribonucleotides in DNA: origins, repair and consequences.

  2014cited by this paper
• RNA intrusions change DNA elastic properties and structure.

  2014cited by this paper
• A Saccharomyces cerevisiae RNase H2 Interaction Network Functions To Suppress Genome Instability

  2014cited by this paper
• Ribonuclease H2 in health and disease.

  2014influential reference
• How the misincorporation of ribonucleotides into genomic DNA can be both harmful and helpful to cells

  2014cited by this paper
• Avoidance of ribonucleotide-induced mutations by RNase H2 and Srs2-Exo1 mechanisms

  2014influential reference
• Sources of DNA double-strand breaks and models of recombinational DNA repair.

  2014cited by this paper
• RNase H2 roles in genome integrity revealed by unlinking its activities

  2013cited by this paper
• Two distinct mechanisms of Topoisomerase 1-dependent mutagenesis in yeast.

  2013cited by this paper
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  2013cited by this paper
• Ribonucleotides Misincorporated into DNA Act as Strand-Discrimination Signals in Eukaryotic Mismatch Repair

  2013cited by this paper
• Aprataxin resolves adenylated RNA-DNA junctions to maintain genome integrity

  2013cited by this paper
• Aicardi–Goutières syndrome: clues from the RNase H2 knock-out mouse

  2013influential reference
• Topoisomerase 1-mediated removal of ribonucleotides from nascent leading-strand DNA.

  2013cited by this paper
• Ribonucleotides are signals for mismatch repair of leading-strand replication errors.

  2013cited by this paper
• RNase H and Postreplication Repair Protect Cells from Ribonucleotides Incorporated in DNA

  2012cited by this paper
• Endogenous DNA replication stress results in expansion of dNTP pools and a mutator phenotype

  2012cited by this paper
• Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae.

  2012cited by this paper
• Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity

  2012influential reference
• Enzymatic Removal of Ribonucleotides from DNA Is Essential for Mammalian Genome Integrity and Development

  2012influential reference
• RNase H2-initiated ribonucleotide excision repair.

  2012cited by this paper
• Solution structure of the Dickerson DNA dodecamer containing a single ribonucleotide.

  2012cited by this paper
• Mismatch repair-independent tandem repeat sequence instability resulting from ribonucleotide incorporation by DNA polymerase ε.

  2011cited by this paper
• RNase H and multiple RNA biogenesis factors cooperate to prevent RNA:DNA hybrids from generating genome instability.

  2011cited by this paper
• Mutagenic Processing of Ribonucleotides in DNA by Yeast Topoisomerase I

  2011cited by this paper
• Highly mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint

  2010cited by this paper
• Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases

  2010cited by this paper
• Genome instability due to ribonucleotide incorporation into DNA

  2010cited by this paper
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  2010cited by this paper
• Role for topoisomerase 1 in transcription-associated mutagenesis in yeast

  2010cited by this paper
• Ribonuclease H: the enzymes in eukaryotes

  2009cited by this paper
• Division of labor at the eukaryotic replication fork.

  2008cited by this paper
• DNA cleavage assay for the identification of topoisomerase I inhibitors

  2008cited by this paper
• In vivo assembly and disassembly of Rad51 and Rad52 complexes during double‐strand break repair

  2004cited by this paper
• Recombination proteins in yeast.

  2004cited by this paper
• Colocalization of multiple DNA double-strand breaks at a single Rad52 repair centre

  2003cited by this paper
• Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts

  2002cited by this paper
• In Vivo Consequences of Putative Active Site Mutations in Yeast DNA Polymerases α, ε, δ, and ζ

  2001cited by this paper
• In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta.

  2001cited by this paper
• From the Cover: Rad52 forms DNA repair and recombination centers during S phase

  2001influential reference
• Mutator Phenotypes Conferred by MLH1Overexpression and by Heterozygosity for mlh1Mutations

  1999cited by this paper
• Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group

  1999cited by this paper
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  1999cited by this paper
• Induction of Reversible Complexes between Eukaryotic DNA Topoisomerase I and DNA-containing Oxidative Base Damages

  1999cited by this paper
• Trapping of Mammalian Topoisomerase I and Recombinations Induced by Damaged DNA Containing Nicks or Gaps

  1997cited by this paper
• Site-specific ribonuclease activity of eukaryotic DNA topoisomerase I.

  1997cited by this paper
• Physiological concentrations of purines and pyrimidines

  1994cited by this paper
• A single 2'-hydroxyl group converts B-DNA to A-DNA. Crystal structure of the DNA-RNA chimeric decamer duplex d(CCGGC)r(G)d(CCGG) with a novel intermolecular G-C base-paired quadruplet.

  1994cited by this paper
• Analysis of the biased distribution of topoisomerase I break sites on replicating simian virus 40 DNA.

  1993cited by this paper
• Effect of local DNA sequence on topoisomerase I cleavage in the presence or absence of camptothecin.

  1991cited by this paper
• Mapping in vivo topoisomerase I sites on simian virus 40 DNA: asymmetric distribution of sites on replicating molecules

  1989cited by this paper

• Application of Biological Modifiers to a Multiplexed, Human Cell‐Based DNA Damage Assay Provides Mechanistic Information on Genotoxicity and Molecular Targets

  2025cites this paper
• Genome-wide ribonucleotide detection in Archaea

  2025cites this paper
• Emerging roles of RNA:DNA hybrid regulation by mammalian ribonuclease H2 in replication stress and cancer

  2025cites this paper
• Endogenous base damage as a driver of genomic instability in homologous recombination-deficient cancers.

  2024cites this paper
• PNKP safeguards stalled replication forks from nuclease-dependent degradation during replication stress.

  2024cites this paper
• Atypical genotoxicity of carcinogenic nickel(II): Linkage to dNTP biosynthesis, DNA-incorporated rNMPs, and impaired repair of TOP1-DNA crosslinks

  2023cites this paper
• Structures of LIG1 uncover the mechanism of sugar discrimination against a ribonucleotide at 3’- and 5’-end of the nick DNA

  2023cites this paper
• RNase H2 degrades toxic RNA:DNA hybrids behind stalled forks to promote replication restart

  2023cites this paper
• Molecular basis for processing of topoisomerase 1-triggered DNA damage by Apn2/APE2

  2022cites this paper
• Structures of LIG1 uncover a lack of sugar discrimination against a ribonucleotide at the 3'-end of nick DNA

  2022influential citation
• Human topoisomerases and their roles in genome stability and organization

  2022cites this paper
• Signatures of TOP1 transcription-associated mutagenesis in cancer and germline

  2022cites this paper
• Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase

  2022cites this paper
• Ribodysgenesis: sudden genome instability in the yeast Saccharomyces cerevisiae arising from RNase H2 cleavage at genomic-embedded ribonucleotides

  2022influential citation
• Ribonucleotide Incorporation by Eukaryotic B-family Replicases and Its Implications for Genome Stability.

  2022cites this paper
• Ribonucleotide incorporation into DNA during DNA replication and its consequences

  2021cites this paper
• Senataxin and RNase H2 act redundantly to suppress genome instability during class switch recombination

  2021cites this paper
• SURVEY AND SUMMARY Mechanisms of damage tolerance and repair during DNA replication

  2021cites this paper
• High Temperature Drives Topoisomerase Mediated Chromosomal Break Repair Pathway Choice

  2021cites this paper
• Mechanisms of damage tolerance and repair during DNA replication

  2021cites this paper
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  2021cites this paper
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  2021cites this paper
• Structural basis for proficient oxidized ribonucleotide insertion in double strand break repair

  2021cites this paper
• High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductase

  2020cites this paper
• RNA: a double-edged sword in genome maintenance

  2020cites this paper
• RNases H1 and H2: guardians of the stability of the nuclear genome when supply of dNTPs is limiting for DNA synthesis

  2020cites this paper
• Origin of Genome Instability and Determinants of Mutational Landscape in Cancer Cells

  2020influential citation
• Next-generation DNA damage sequencing.

  2020cites this paper
• Tyrosyl-DNA phosphodiesterases are involved in mutagenic events at a ribonucleotide embedded into DNA in human cells

  2020cites this paper
• The Functional Consequences of Eukaryotic Topoisomerase 1 Interaction with G-Quadruplex DNA

  2020cites this paper
• Topoisomerase I-driven repair of UV-induced damage in NER-deficient cells

  2020cites this paper
• RNase H1 and H2 Are Differentially Regulated to Process RNA-DNA Hybrids.

  2019cites this paper
• SAMHD1 and the innate immune response to cytosolic DNA during DNA replication.

  2019cites this paper
• Current perspectives on mechanisms of ribonucleotide incorporation and processing in mammalian DNA

  2019cites this paper
• RNase H activities counteract a toxic effect of Polymerase η in cells replicating with depleted dNTP pools

  2019cites this paper
• Genome instability consequences of RNase H2 Aicardi-Goutières syndrome alleles.

  2019cites this paper
• RNase H1 and H2 are differentially regulated to eliminate RNA-DNA hybrids

  2019cites this paper
• Genome-wide mutagenesis resulting from topoisomerase 1-processing of unrepaired ribonucleotides in DNA.

  2019cites this paper
• DNA Damage by an essential enzyme: A delicate balance act on the tightrope.

  2019cites this paper
• Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks.

  2019cites this paper
• Processing of a single ribonucleotide embedded into DNA by human nucleotide excision repair and DNA polymerase η

  2019cites this paper
• Topoisomerases and cancer chemotherapy: recent advances and unanswered questions

  2019cites this paper
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  2019influential citation
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  2019cites this paper
• Replication Stress and Consequential Instability of the Genome and Epigenome

  2019cites this paper
• RNase H2-RED carpets the path to eukaryotic RNase H2 functions.

  2019influential citation
• Differential Activities of DNA Polymerases in Processing Ribonucleotides during DNA Synthesis in Archaea.

  2018cites this paper
• Ribonucleotide Excision Repair Is Essential to Prevent Squamous Cell Carcinoma of the Skin.

  2018cites this paper
• CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions

  2018cites this paper
• Dihydropyrimidinase protects from DNA replication stress caused by cytotoxic metabolites

  2018cites this paper
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  2017cites this paper
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• Cytosine deamination and base excision repair cause R-loop–induced CAG repeat fragility and instability in Saccharomyces cerevisiae

  2017cites this paper
• The Top1 paradox: Friend and foe of the eukaryotic genome.

  2017cites this paper
• Telomere Length Determines TERRA and R-Loop Regulation through the Cell Cycle.

  2017cites this paper
• Topoisomerase I‐mediated cleavage at unrepaired ribonucleotides generates DNA double‐strand breaks

  2017cites this paper
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  2017cites this paper
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  2017cites this paper
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  2017cites this paper
• Genome instabilities arising from ribonucleotides in DNA.

  2017cites this paper
• The role of RNase H2 in processing ribonucleotides incorporated during DNA replication.

  2017cites this paper
• Characterization of Nucleic Acids and Nucleic Acid Binding Proteins Using NMR

  2017cites this paper