Uniform Serial Sectioning for Transmission Electron Microscopy

The transmission electron microscope (TEM) was first used approximately half a century ago to answer important neurobiological questions, showing unequivocally that neurons communicate via synaptic junctions ([Palay and Palade, 1955][1]; [Gray, 1959][2]). TEM usually requires that biological

• Publication year

  2006

• Venue

  Journal of Neuroscience

• Publication date

  2006-11-22

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1523/JNEUROSCI.3994-06.2006PMID 17122034PMCID PMC6675417
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Spine growth precedes synapse formation in the adult neocortex in vivo

  2006cited by this paper
• Towards neural circuit reconstruction with volume electron microscopy techniques.

  2006cited by this paper
• Polyribosomes redistribute from dendritic shafts into spines with enlarged synapses during LTP in developing rat hippocampal slices.

  2002cited by this paper
• Preservation of neuronal ultrastructure in hippocampal slices using rapid microwave-enhanced fixation.

  1989cited by this paper
• A systematic approach to reconstructing microcircuitry by electron microscopy of serial sections.

  1980cited by this paper
• Ultrastructure and three-dimensional organization of synaptic glomeruli in rat somatosensory thalamus.

  1974cited by this paper
• Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study.

  1959cited by this paper
• Graphic reconstruction of the third dimension from serial electron microphotographs.

  1957cited by this paper
• Reconstruction of the nuclear sites of Salmonella typhimurium from electron micrographs of serial sections.

  1955cited by this paper
• THE FINE STRUCTURE OF NEURONS

  1955cited by this paper

• Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.

  2026cites this paper
• Perisynaptic Astroglial Response to In Vivo Long-Term Potentiation and Concurrent Long-Term Depression in the Hippocampal Dentate Gyrus

  2025cites this paper
• Perisynaptic astroglial response to in vivo long-term potentiation and concurrent long-term depression in the hippocampal dentate gyrus

  2025cites this paper
• Pcdh20 is a POU2F3 target gene required for proper tuft cell microvillus organization

  2025cites this paper
• PyReconstruct: A fully open-source, collaborative successor to Reconstruct

  2025cites this paper
• Multiplexed genetic tags for electron and fluorescence microscopy.

  2025cites this paper
• A workflow for semi‐automated volume correlative light microscopy and transmission electron tomography

  2025cites this paper
• ssEM Image Restoration via Diffusion Models With Multi-Output Joint Strategy for Noise Estimation

  2025cites this paper
• Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microscopy Imaging of Single Stimulated Synapses.

  2025cites this paper
• Two- and three-dimensional electron microscopy techniques: powerful tools for studying the brain under physiological and pathological conditions

  2024cites this paper
• Array tomography of in vivo labeled synaptic receptors.

  2024cites this paper
• A robust transformer-based pipeline of 3D cell alignment, denoise and instance segmentation on electron microscopy sequence images.

  2024cites this paper
• Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution

  2024cites this paper
• Array tomography: trails to discovery

  2024cites this paper
• 3D Reconstruction of the Mitochondrial Network within the Neuronal Soma from SBF-SEM Volume Data.

  2024cites this paper
• Long-Term Potentiation Produces a Sustained Expansion of Synaptic Information Storage Capacity in Adult Rat Hippocampus

  2024cites this paper
• iTome Volumetric Serial Sectioning Apparatus for TEM

  2024cites this paper
• Cross-dimension Affinity Distillation for 3D EM Neuron Segmentation

  2024cites this paper
• Ultrastructural 3D Microscopy for Biomedicine: Principles, Applications, and Perspectives

  2024cites this paper
• Quantitative Geometric Modeling of Blood Cells from X-ray Histotomograms of Whole Zebrafish Larvae

  2023cites this paper
• Roadmap for focused ion beam technologies

  2023cites this paper
• Neurons on tape: Automated Tape Collecting Ultramicrotomy-mediated volume EM for targeting neuropathology.

  2023cites this paper
• Techniques to Render Dendritic Spines Visible in the Microscope.

  2023cites this paper
• Regional and LTP-Dependent Variation of Synaptic Information Storage Capacity in Rat Hippocampus

  2023cites this paper
• Quantifying Intracellular Nanoparticle Distributions with Three-Dimensional Super-Resolution Microscopy.

  2023cites this paper
• CellRemorph: A Toolkit for Transforming, Selecting, and Slicing 3D Cell Structures on the Road to Morphologically Detailed Astrocyte Simulations

  2023cites this paper
• Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy

  2023cites this paper
• Induction of lysosomal and mitochondrial biogenesis by AMPK phosphorylation of FNIP1

  2023cites this paper
• Synergistic Effect of Cypermethrin and Sodium Fluoride on Kidney Histo Pathology of Albino Mice

  2023cites this paper
• Liver inter-organelle membrane contact sites revealed by serial section electron tomography.

  2023cites this paper
• PS-Net: human perception-guided segmentation network for EM cell membrane

  2023cites this paper
• A practical guide to starting SEM array tomography-An accessible volume EM technique.

  2023cites this paper
• Semi-Supervised Neuron Segmentation via Reinforced Consistency Learning

  2022cites this paper
• Deep residual contextual and subpixel convolution network for automated neuronal structure segmentation in micro-connectomics

  2022cites this paper
• Neuronal Morphological Model-Driven Image Registration for Serial Electron Microscopy Sections

  2022cites this paper
• Dendritic spine density scales with microtubule number in rat hippocampal dendrites.

  2022cites this paper
• Petascale pipeline for precise alignment of images from serial section electron microscopy

  2022cites this paper
• Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy.

  2022cites this paper
• A Unified Deep Learning Framework for ssTEM Image Restoration

  2022cites this paper
• Evaluation of the validity of large-scale serial sectioning TEM for three-dimensional reconstruction of rice mesophyll cells and chloroplasts

  2022cites this paper
• Volumetric 3D reconstruction of plant leaf cells using SEM, ion milling, TEM, and serial sectioning

  2022cites this paper
• How innovations in methodology offer new prospects for volume electron microscopy

  2022cites this paper
• U-RISC: An Ultra-high Resolution EM 1 Dataset Challenging Existing Deep 2 Learning Algorithms 3

  2021cites this paper
• Three-Dimensional Structure of Dendritic Spines Revealed by Volume Electron Microscopy Techniques

  2021cites this paper
• Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM

  2021cites this paper
• Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

  2021cites this paper
• 1 Single-cell transcriptomics reveals a conserved metaplasia program in pancreatic injury

  2021cites this paper
• Single-cell transcriptomics reveals a conserved metaplasia program in pancreatic injury

  2021cites this paper
• Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

  2021cites this paper
• Preparation of Samples for Large-Scale Automated Electron Microscopy of Tissue and Cell Ultrastructure

  2021influential citation
• Electron Microscopy Techniques for Investigating Structure and Composition of Hair-Cell Stereociliary Bundles

  2021cites this paper
• Advanced Technologies for Local Neural Circuits in the Cerebral Cortex

  2021cites this paper
• Preparation of large-scale digitization samples for automated electron microscopy of tissue and cell ultrastructure

  2021cites this paper
• HUMAN PERCEPTION-BASED EVALUATION CRITE-

  2020cites this paper
• Volume electron microscopy: analyzing the lung

  2020cites this paper
• Ultra-Microtome for the Preparation of TEM Specimens from Battery Cathodes

  2020cites this paper
• Human Perception-based Evaluation Criterion for Ultra-high Resolution Cell Membrane Segmentation

  2020cites this paper
• Learning to Restore ssTEM Images from Deformation and Corruption

  2020cites this paper
• Transforming FIB-SEM Systems for Large-Volume and

  2020cites this paper
• New insights in osteocyte imaging by synchrotron radiation

  2020cites this paper
• Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits

  2020cites this paper
• Capillary-Based and Stokes-Based Trapping of Serial Sections for Scalable 3D-EM Connectomics

  2020cites this paper
• Probing the Ultrastructure of Spheroids and Their Uptake of Magnetic Nanoparticles by FIB–SEM

  2020cites this paper
• A Low-Tech Approach to Serial Arrays

  2020cites this paper
• Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions

  2020cites this paper
• Synaptic Odyssey

  2020cites this paper
• Serial block-face scanning electron microscopy: A provocative technique to define 3-dimensional ultrastructure of microvascular thrombosis.

  2020cites this paper
• Combined membrane potential imaging and connectome of behavioral circuits in an annelid worm

  2020cites this paper
• Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology

  2020cites this paper
• OBSOLETE: The Senses: Retinal Connectomics

  2020cites this paper
• Skeleton-based image registration of serial electron microscopy sections

  2019cites this paper
• Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation

  2019cites this paper
• Serial block-face scanning electron microscopy reveals neuronal-epithelial cell fusion in the mouse cornea

  2019cites this paper
• A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy

  2019cites this paper
• MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

  2019cites this paper
• 3D mesh processing using GAMer 2 to enable reaction-diffusion simulations in realistic cellular geometries

  2019cites this paper
• Structural plasticity of dendritic secretory compartments during LTP-induced synaptogenesis

  2019cites this paper
• GAMer 2: A system for 3D mesh processing of cellular electron micrographs

  2019cites this paper
• The viral replication organelles within cells studied by electron microscopy

  2019cites this paper
• 3D CLEM

  2019cites this paper
• Multiscale structures in particle–fluid systems: Characterization, modeling, and simulation

  2019cites this paper
• Immunogold labeling of synaptic vesicle proteins in developing hippocampal neurons

  2019cites this paper
• Enabling FIB-SEM Systems for Large Volume Connectomics and Cell Biology

  2019cites this paper
• A carbon nanotube tape for serial-section electron microscopy of brain ultrastructure

  2018cites this paper
• Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

  2018cites this paper
• Multi-Beam Scanning Electron Microscopy for High-Throughput Imaging in Connectomics Research

  2018cites this paper
• Methodological Improvements With Conductive Materials for Volume Imaging of Neural Circuits by Electron Microscopy

  2018cites this paper
• An Adversarial and Densely Dilated Network for Connectomes Segmentation

  2018cites this paper
• Automatic Mitochondria Segmentation for EM Data Using a 3D Supervised Convolutional Network

  2018cites this paper
• Large-scale neuroanatomy using LASSO: Loop-based Automated Serial Sectioning Operation

  2018cites this paper
• Morphology-Retained Non-Linear Image Registration of Serial Electron Microscopy Sections

  2018cites this paper
• Thrombospondin receptor α2δ-1 promotes synaptogenesis and spinogenesis via postsynaptic Rac1

  2018cites this paper
• Effective automated pipeline for 3D reconstruction of synapses based on deep learning

  2018cites this paper
• FIB‐SEM imaging properties of Drosophila melanogaster tissues embedded in Lowicryl HM20

  2018cites this paper
• Loss and remodeling of striatal dendritic spines in Parkinson’s disease: from homeostasis to maladaptive plasticity?

  2018cites this paper
• Structure and diversity of human dendritic spines evidenced by a new three-dimensional reconstruction procedure for Golgi staining and light microscopy.

  2018cites this paper
• Technologies for Cellular-Resolution Connectomics

  2018cites this paper
• Long-term potentiation expands information content of hippocampal dentate gyrus synapses

  2018cites this paper
• Electron tomography of whole cultured cells using novel transmission electron imaging technique.

  2018cites this paper
• Comparison of methods for the characterization of voids in glass fiber composites

  2018cites this paper