Uptake and metabolism of ammonium and nitrate in response to drought stress in Malus prunifolia.

Using a hydroponics culture system, we monitored morphological, physiological, and molecular changes in Malus prunifolia seedlings when drought conditions induced by 5% polyethylene glycol (PEG) were combined with a low or normal supply of N (0.05 mM or 1 mM NH4NO3, respectively). Under either nutrient level, drought stress negatively inhibited seedling performance, as manifested by reduced photosynthesis and biomass production, decreased accumulations of total N, and inhibited root growth. Concentrations of NO3- and NH4+ and the activities of enzymes involved in N metabolism (nitrate reductase, glutamine synthetase, and glutamate synthase) were also significantly decreased under drought stress. The net influx of NO3- at the surface of the fine roots declined while that of NH4+ rose markedly, suggesting that the latter may play a more important role in improving drought tolerance in M. prunifolia. Consistently, two ammonium transporters (AMT1;2 and AMT4;2) were notably up-regulated in response to drought stress, whereas most genes related to nitrate uptake, reduction, and N metabolism were down-regulated. At the normal N level, PEG-treated plants showed higher values for biomass production, root growth, and N uptake/reduction when compared with plants exposed to the lower N supply. These results suggest that the negative effect of drought stress on M. prunifolia may be alleviated when more nitrogen is available.

• Publication year

  2018

• Venue

  Plant physiology and biochemistry : PPB

• Publication date

  2018-06-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.plaphy.2018.03.031PMID 29609174
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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