Physical and biological properties of cationic triesters of phosphatidylcholine.

The properties of a new class of phospholipids, alkyl phosphocholine triesters, are described. These compounds were prepared from phosphatidylcholines through substitution of the phosphate oxygen by reaction with alkyl trifluoromethylsulfonates. Their unusual behavior is ascribed to their net positive charge and absence of intermolecular hydrogen bonding. The O-ethyl, unsaturated derivatives hydrated to generate large, unilamellar liposomes. The phase transition temperature of the saturated derivatives is very similar to that of the precursor phosphatidylcholine and quite insensitive to ionic strength. The dissociation of single molecules from bilayers is unusually facile, as revealed by the surface activity of aqueous liposome dispersions. Vesicles of cationic phospholipids fused with vesicles of anionic lipids. Liquid crystalline cationic phospholipids such as 1, 2-dioleoyl-sn-glycero-3-ethylphosphocholine triflate formed normal lipid bilayers in aqueous phases that interacted with short, linear DNA and supercoiled plasmid DNA to form a sandwich-structured complex in which bilayers were separated by strands of DNA. DNA in a 1:1 (mol) complex with cationic lipid was shielded from the aqueous phase, but was released by neutralizing the cationic charge with anionic lipid. DNA-lipid complexes transfected DNA into cells very effectively. Transfection efficiency depended upon the form of the lipid dispersion used to generate DNA-lipid complexes; in the case of the O-ethyl derivative described here, large vesicle preparations in the liquid crystalline phase were most effective.

• Publication year

  1999

• Venue

  Biophysical Journal

• Publication date

  1999-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0006-3495(99)77095-5PMID 10545361PMCID PMC1300535
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Cationic phospholipid dispersions formed DNA-lipid complexes that transfected cells very effectively, with large vesicle preparations in the liquid crystalline phase giving the best efficiency.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• Saturated alkyl phosphocholine triesters showed phase transition temperatures very similar to their phosphatidylcholine precursors and were largely insensitive to ionic strength.

  Confidence 0.93

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• The O-ethyl unsaturated alkyl phosphocholine triesters hydrated to form large unilamellar liposomes.

  Confidence 0.95

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• Alkyl phosphocholine triesters were synthesized from phosphatidylcholines by substituting a phosphate oxygen with alkyl trifluoromethylsulfonates.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review

• alkyl phosphocholine triesters

  chemical class

  A class of phospholipid derivatives bearing an alkyl phosphocholine triester headgroup.

  Aliases: cationic triesters of phosphatidylcholine

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• alkyl trifluoromethylsulfonates

  reagent, chemical compound

  Alkylating reagents used to replace phosphate oxygen during triester synthesis.

  Aliases: alkyl triflates

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• cationic phospholipids

  lipid class

  Phospholipid molecules carrying a net positive charge in their headgroup region.

  Aliases: cationic lipid

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• dna-lipid complexes

  complex

  Assemblies formed when cationic lipid bilayers interact with DNA strands or plasmids.

  Aliases: lipoplexes

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• large unilamellar liposomes

  liposome, vesicle

  Single-bilayer vesicles formed by hydrating selected lipid derivatives in aqueous solution.

  Aliases: LUVs

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• liquid crystalline phase

  phase

  A fluid bilayer state of lipid assemblies used here to characterize vesicle dispersions.

  Aliases: Lalpha phase

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review
• phosphatidylcholines

  lipid class

  The parent phospholipids used as starting materials for making the triester derivatives.

  Aliases: PCs

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) reviewKiller Whale (322360f1c1) review

• Factors governing the assembly of cationic phospholipid-DNA complexes.

  2000cited by this paper
• O-ethylphosphatidylcholine: A metabolizable cationic phospholipid which is a serum-compatible DNA transfection agent.

  1999influential reference
• Directly Observed Membrane Fusion Between Oppositely Charged Phospholipid Bilayers

  1999cited by this paper
• A microplate fluorimetric assay for transfection of the beta-galactosidase reporter gene.

  1998cited by this paper
• An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery.

  1998influential reference
• Membrane fusion with cationic liposomes: effects of target membrane lipid composition.

  1997cited by this paper
• Efficient in vivo delivery of DNA to pulmonary cells using the novel lipid EDMPC

  1997cited by this paper
• Structure of DNA-Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes

  1997cited by this paper
• The Structure of DNA−Liposome Complexes

  1997cited by this paper
• The effect of lipid molecular packing stress on cationic liposome-induced rabbit erythrocyte fusion.

  1997cited by this paper
• Biophysical characterization of cationic lipid: DNA complexes.

  1997influential reference
• Structure of in‐serum transfecting DNA–cationic lipid complexes

  1997cited by this paper
• Delivery of membrane-impermeant fluorescent probes into living neural cell populations by lipotransfer.

  1996cited by this paper
• Converting an alcohol to an amine in a cationic lipid dramatically alters the co-lipid requirement, cellular transfection activity and the ultrastructure of DNA-cytofectin complexes.

  1996cited by this paper
• THE RELATIONSHIP AND INTERACTIONS BETWEEN LIPID BILAYERS VESICLES AND LIPID MONOLAYERS AT THE AIR/WATER INTERFACE

  1996cited by this paper
• Morphology of Cationic Liposome/DNA Complexes in Relation to Their Chemical Composition

  1996cited by this paper
• [Human gene therapy].

  1996cited by this paper
• Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.

  1996cited by this paper
• The counterion influence on cationic lipid-mediated transfection of plasmid DNA.

  1996cited by this paper
• Efficient lipid-mediated transfection of DNA into primary rat hepatocytes

  1995cited by this paper
• Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid (*)

  1995cited by this paper
• A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

  1995cited by this paper
• Engineering liposomes for drug delivery: progress and problems.

  1995cited by this paper
• Endosomolytic activity of cationic liposomes enhances the delivery of human immunodeficiency virus-1 trans-activator protein (TAT) to mammalian cells.

  1995cited by this paper
• A novel series of amphiphilic imidazolinium compounds for in vitro and in vivo gene delivery.

  1995cited by this paper
• The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer.

  1995influential reference
• Experimental tests for protrusion and undulation pressures in phospholipid bilayers.

  1995cited by this paper
• Complexes between cationic liposomes and DNA visualized by cryo-TEM.

  1995cited by this paper
• The evolving role of the liposome in gene delivery:Historical and Conceptual Background

  1995cited by this paper
• A mild procedure for etherification of alcohols with primary alkyl halides in the presence of silver triflate

  1994cited by this paper
• Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations.

  1994cited by this paper
• Design of a new cationic amphiphile with efficient DNA-transfection ability.

  1994cited by this paper
• A metastable state of high surface activity produced by sonication of phospholipids.

  1994cited by this paper
• Cationic Liposomes for Direct Gene Transfer in Therapy of Cancer and Other Diseases a

  1994cited by this paper
• New structures in complex formation between DNA and cationic liposomes visualized by freeze—fracture electron microscopy

  1994cited by this paper
• Liposome-mediated cytoplasmic delivery of proteins: an effective means of accessing the MHC class I-restricted antigen presentation pathway.

  1994cited by this paper
• Introduction of hepatitis delta virus into animal cell lines via cationic liposomes

  1994cited by this paper
• Human gene therapy.

  1993cited by this paper
• Gene Transfer Optimization with Lipospermine-Coated DNA

  1993influential reference
• Mode of formation and structural features of DNA-cationic liposome complexes used for transfection.

  1993influential reference
• Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides.

  1992cited by this paper
• A novel cationic liposome reagent for efficient transfection of mammalian cells.

  1991influential reference
• Regulation of gene expression in vivo by liposome-mediated delivery of a purified transcription factor.

  1990cited by this paper
• Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles.

  1990influential reference
• Fusion of liposomes containing a novel cationic lipid, N-[2,3-(dioleyloxy)propyl]-N,N,N-trimethylammonium: induction by multivalent anions and asymmetric fusion with acidic phospholipid vesicles.

  1989cited by this paper
• Cholesterol modifies the short-range repulsive interactions between phosphatidylcholine membranes.

  1989cited by this paper
• Interactions of cationic lipid vesicles with negatively charged phospholipid vesicles and biological membranes.

  1988influential reference
• Steric repulsion between phosphatidylcholine bilayers.

  1987cited by this paper
• Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

  1987cited by this paper
• Lipid monolayer states and their relationships to bilayers.

  1987cited by this paper
• Use of a recombinant retrovirus to study post‐implantation cell lineage in mouse embryos.

  1986cited by this paper
• Hydration force and bilayer deformation: a reevaluation.

  1986cited by this paper
• Thermodynamics and kinetics of phospholipid monomer-vesicle interaction.

  1985cited by this paper
• Thermodynamics and kinetics of phospholipid monomer-vesicle interaction.

  1985cited by this paper
• Thermodynamics and kinetics of protein incorporation into membranes.

  1983cited by this paper
• Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles.

  1983cited by this paper
• The three-dimensional structure of DNA.

  1982cited by this paper
• A fluorescence assay to monitor vesicle fusion and lysis.

  1982cited by this paper
• Perfluoroalkanesulfonic Esters: Methods of Preparation and Applications in Organic Chemistry

  1982cited by this paper
• Small DNA-free liposomes stimulate transfection of streptomyces protoplasts

  1982cited by this paper
• Use of resonance energy transfer to monitor membrane fusion.

  1981cited by this paper
• The influence of charge on bilayer membranes. Calorimetric investigations of phosphatidic acid bilayers.

  1979cited by this paper
• Calorimetric Investigations of Phosphatidic Acid Bilayers

  1978cited by this paper
• Electrostatic interactions at charged lipid membranes. Measurement of surface pH with fluorescent lipoid pH indicators.

  1978cited by this paper
• INTERACTIONS BETWEEN LIPID VESICLES AND CELL MEMBRANES *

  1978cited by this paper
• A direct analysis of lamellar x-ray diffraction from hydrated oriented multilayers of fully functional sarcoplasmic reticulum.

  1977influential reference
• Lipid vesicle-cell interactions. III. Introduction of a new antigenic determinant into erythrocyte membranes

  1976cited by this paper
• Electrostatic interactions at charged lipid membranes. I. Effects of pH and univalent cations on membrane structure.

  1976cited by this paper
• Ionic influences on the phase transition of dipalmitoylphosphatidylserine.

  1976influential reference
• Phospholipid exchange between bilayer membrane vesicles.

  1976cited by this paper
• A comparison of negatively and positively charged liposomes containing entrapped polyinosinic-polycytidylic acid for interferon induction in mice.

  1976cited by this paper
• Lipid vesicle-cell interactions. II. Induction of cell fusion

  1976cited by this paper
• Liposomes can mimic virus membranes

  1974cited by this paper
• Interferon Induction by Poly(I):Poly(C) Enclosed in Phospholipid Particles

  1974cited by this paper
• Structure and polymorphism of the hydrocarbon chains of lipids: a study of lecithin-water phases.

  1973cited by this paper
• Temperature dependence of optical properties of aqueous dispersions of phosphatidylcholine

  1973cited by this paper
• Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. I. Use of spin labels and optical probes as indicators of the phase transition.

  1972cited by this paper
• Bilayer structure in membranes.

  1971cited by this paper
• Demonstration of blocking the carboxyl moiety in the active center of pepsin by diazocarbonyl inhibitor

  1969cited by this paper
• Treatment of low angle x-ray data from planar and concentric multilayered structures.

  1966influential reference
• Diffusion of univalent ions across the lamellae of swollen phospholipids.

  1965cited by this paper
• Phosphorus assay in column chromatography.

  1959cited by this paper

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  2024cites this paper
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  2023influential citation
• Study of the correlation between the structure of selected triester of phosphatidylcholine and their impact on physicochemical properties of model mammalian membranes.

  2023cites this paper
• Lipid Nanoparticles-From Liposomes to mRNA Vaccine Delivery, a Landscape of Research Diversity and Advancement.

  2021cites this paper
• Mucus-producing epithelial models for investigating the activity of gene delivery systems in the lung.

  2021cites this paper
• Lipids and Lipid Derivatives for RNA Delivery.

  2021cites this paper
• The influence of cationic lipoid - 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine - on model lipid membranes.

  2020cites this paper
• Cationic liposomes for generic signal amplification strategies in bioassays

  2020cites this paper
• Reorganization from Kinetically Stable Aggregation States to Thermodynamically Stable Nanotubes of BINOL-Derived Amphiphiles in Water

  2019cites this paper
• Salen‐Based Amphiphiles: Directing Self‐Assembly in Water by Metal Complexation

  2019cites this paper
• Studies on the Interactions of 2-Hydroxyoleic Acid with Monolayers and Bilayers Containing Cationic Lipid: Searching for the Formulations for More Efficient Drug Delivery to Cancer Cells.

  2019cites this paper
• Relevance of the protein macrodipole in the membrane-binding process. Interactions of fatty-acid binding proteins with cationic lipid membranes

  2018cites this paper
• Influence of Cationic Phosphatidylcholine Derivative on Monolayer and Bilayer Artificial Bacterial Membranes.

  2018cites this paper
• Coil-globule structure transition and binding characteristics of DNA molecules induced by isoquinoline-based photoactive ionic liquid surfactant

  2017cites this paper
• Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.

  2017cites this paper
• Cubic phases in phosphatidylethanolamine dispersions: Formation, stability and phase transitions.

  2017cites this paper
• A modular platform for one-step assembly of multi-component membrane systems by fusion of charged proteoliposomes

  2016cites this paper
• Nonlamellar Phases in Cationic Phospholipids, Relevance to Drug and Gene Delivery.

  2015cites this paper
• Synthetic nucleic acid delivery systems: present and perspectives.

  2015cites this paper
• The interdigitated gel phase in mixtures of cationic and zwitterionic phospholipids.

  2015cites this paper
• A lipid fusion based method for the single molecule study of ATP synthase

  2014cites this paper
• Enhancing nucleic acid delivery, insights from the cationic phospholipid carriers.

  2014cites this paper
• Molecular dynamics shows that ion pairing and counterion anchoring control the properties of triflate micelles: a comparison with triflate at the air/water interface.

  2014cites this paper
• AH peptide-mediated formation of charged planar lipid bilayers.

  2014cites this paper
• A RESPONSIVE PARTICLE COATING FOR EMULSION FORMATION AT THE OIL-WATER INTERFACE

  2014influential citation
• Pyridinium-based cationic lipids: correlations of molecular structure with nucleic acid transfection efficiency

  2014cites this paper
• Enhanced Intrapulmonary Delivery of Anticancer siRNA for Lung Cancer Therapy Using Cationic Ethylphosphocholine-based Nanolipoplexes

  2013cites this paper
• Lipid-mediated DNA and siRNA Transfection Efficiency Depends on Peptide Headgroup.

  2013cites this paper
• Synthesis, self-assembling and gene delivery potential of a novel highly unsaturated, conjugated cationic phospholipid.

  2013cites this paper
• Differential Scanning Calorimetry Studies of Phospholipid Membranes: The Interdigitated Gel Phase

  2013cites this paper
• Bioresponsive Deciduous-Charge Amphiphiles for Liposomal Delivery of DNA and siRNA

  2013influential citation
• Phospholipid-detergent conjugates as novel tools for siRNA delivery.

  2013cites this paper
• Highly efficient cationic ethylphosphatidylcholine siRNA carrier for GFP suppression in modified breast cancer cells.

  2012cites this paper
• Distinct initial SNARE configurations underlying the diversity of exocytosis.

  2012cites this paper
• The Role of Supramolecular Chemistry in Responsive Vectors for Gene Delivery

  2012cites this paper
• A cationic phospholipid-detergent conjugate as a new efficient carrier for siRNA delivery.

  2012cites this paper
• Functional lipids and lipoplexes for improved gene delivery.

  2012cites this paper
• Light and host-guest inclusion mediated salmon sperm DNA/surfactant interactions.

  2011cites this paper
• Polyamine – Lipid Conjugates as Effective Gene Carriers: Chemical Structure, Morphology, and Gene Transfer Activity

  2011influential citation
• Neutral Liposomes and DNA Transfection

  2011cites this paper
• DNA-lipid self-assembly: phase behavior and phase structures of a DNA-surfactant complex mixed with lecithin and water

  2011cites this paper
• Gene vectors based on DOEPC/DOPE mixed cationic liposomes: a physicochemical study

  2011cites this paper
• Cationic phospholiposomes: efficient delivery vehicles of anticancer derivatives of ATP to multiple myeloma cells

  2011cites this paper
• Nanoparticle assemblies for biological and chemical sensing

  2010cites this paper
• Cationic lipids: molecular structure/ transfection activity relationships and interactions with biomembranes.

  2010influential citation
• Analysis of lipoplex structure and lipid phase changes.

  2010cites this paper
• Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.

  2010cites this paper
• The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.

  2010cites this paper
• Binding characteristics and molecular mechanism of interaction between ionic liquid and DNA.

  2010cites this paper
• Effect of lipid composition on the structure and theoretical phase diagrams of DC-Chol/DOPE-DNA lipoplexes.

  2010cites this paper
• Liposomal polyamine-dialkyl phosphate conjugates as effective gene carriers: chemical structure, morphology, and gene transfer activity.

  2010cites this paper
• Gene Therapy with Plasmid DNA

  2010cites this paper
• Nanoparticle assembly for 1D and 2D ordered structures

  2009cites this paper
• Cationic phospholipids: structure transfection activity relationships

  2009cites this paper
• Factors controlling the stability of a kinetically hindered lamellar-lamellar transition.

  2009cites this paper
• Advances in gene delivery through molecular design of cationic lipids.

  2009cites this paper
• A theoretical and experimental approach to the compaction process of DNA by dioctadecyldimethylammonium bromide/zwitterionic mixed liposomes.

  2009cites this paper
• Identification of tight junction modulating lipids.

  2009cites this paper
• Cationic nucleoside lipids based on a 3-nitropyrrole universal base for siRNA delivery.

  2009cites this paper
• Hydrophobic moiety of cationic lipids strongly modulates their transfection activity.

  2009cites this paper
• Cell transfection by DNA-lipid complexes — Lipoplexes

  2009cites this paper
• Electrochemical and spectroscopic study of octadecyltrimethylammonium bromide/DNA surfoplexes.

  2009cites this paper
• Compaction process of calf thymus DNA by mixed cationic-zwitterionic liposomes: a physicochemical study.

  2008cites this paper
• Non-linear pharmacodynamics in the transfection efficiency of a non-viral gene delivery system.

  2008cites this paper
• Enhanced transfection efficiency of multicomponent lipoplexes in the regime of optimal membrane charge density.

  2008cites this paper
• Lipophilic peptides for gene delivery.

  2008cites this paper
• A physicochemical characterization of the interaction between DC-Chol/DOPE cationic liposomes and DNA.

  2008cites this paper
• Modulation of a membrane lipid lamellar-nonlamellar phase transition by cationic lipids: a measure for transfection efficiency.

  2008cites this paper
• Physicochemical parameters of non-viral vectors that govern transfection efficiency.

  2008cites this paper
• Lipid Phases Eye View to Lipofection. Cationic Phosphatidylcholine Derivatives as Efficient DNA Carriers for Gene Delivery

  2008cites this paper
• A new helper phospholipid for gene delivery.

  2008cites this paper
• Cationic phospholipids forming cubic phases: lipoplex structure and transfection efficiency.

  2008cites this paper
• Characterization of DODAB/DPPC vesicles.

  2008cites this paper
• Natural lipid extracts and biomembrane-mimicking lipid compositions are disposed to form nonlamellar phases, and they release DNA from lipoplexes most efficiently.

  2007cites this paper
• Synergy in lipofection by cationic lipid mixtures: superior activity at the gel-liquid crystalline phase transition.

  2007cites this paper
• Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA.

  2007cites this paper
• 2D aggregation and selective desorption of nanoparticle probes: a new method to probe DNA mismatches and damages.

  2007cites this paper
• Single lipoplex study of cationic lipoid-DNA, self-assembled complexes.

  2007cites this paper
• Structural stability against disintegration by anionic lipids rationalizes the efficiency of cationic liposome/DNA complexes.

  2007cites this paper
• Synergistic effect between components of mixtures of cationic amphipaths in transfection of primary endothelial cells.

  2007cites this paper
• Lateral organization of a membrane protein in a supported binary lipid domain: direct observation of the organization of bacterial light-harvesting complex 2 by total internal reflection fluorescence microscopy.

  2006cites this paper
• An intracellular lamellar–nonlamellar phase transition rationalizes the superior performance of some cationic lipid transfection agents

  2006cites this paper
• Transfection activity of binary mixtures of cationic o-substituted phosphatidylcholine derivatives: the hydrophobic core strongly modulates physical properties and DNA delivery efficacy.

  2006cites this paper
• Surface properties of dioleoyl-sn-glycerol-3-ethylphosphocholine, a cationic phosphatidylcholine transfection agent, alone and in combination with lipids or DNA.

  2006cites this paper
• DNA detection method based on the two-dimensional aggregation and selective desorption of nanoparticle probes.

  2006cites this paper
• Phospholipids and the origin of cationic gating charges in voltage sensors

  2006cites this paper
• Basic and clinical aspects of gene therapy for retinopathy induced by diabetes.

  2006cites this paper
• Lipid phase control of DNA delivery.

  2005cites this paper
• Negative dipole potentials of uncharged langmuir monolayers due to fluorination of the hydrophilic heads.

  2005cites this paper
• Analysis of the structure and composition of individual lipoplex particles by flow fluorometry.

  2005cites this paper
• Lipid transfer between cationic vesicles and lipid-DNA lipoplexes: effect of serum.

  2005cites this paper
• Nucleoside phosphocholine amphiphile for in vitro DNA transfection.

  2005cites this paper
• Novel molecular approaches to cystic fibrosis gene therapy.

  2005cites this paper
• Reactifs de transfection

  2004cites this paper
• Compositional effects of cationic lipid/DNA delivery systems on transgene expression in cell culture.

  2004cites this paper
• Regulation of PutA-membrane associations by flavin adenine dinucleotide reduction.

  2004cites this paper
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  2004cites this paper
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  2004cites this paper
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