New constructs and strategies for efficient PCR‐based gene manipulations in yeast

No abstract is available for this paper.

• Publication year

  1998

• Venue

  Yeast

• Publication date

  1998-09-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1002/(SICI)1097-0061(19980915)14:12<1139::AID-YEA306>3.0.CO;2-BPMID 9778799
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1998cited by this paper
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  1997influential reference
• One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins.

  1996cited by this paper
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  1996cited by this paper
• PCR‐synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae

  1996cited by this paper
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  1996cited by this paper
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  1996cited by this paper
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  1995influential reference
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  1995cited by this paper
• A highly conserved ATPase protein as a mediator between acidic activation domains and the TATA-binding protein

  1995cited by this paper
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  1995cited by this paper
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  1995influential reference
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  1995influential reference
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  1994cited by this paper
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  1994cited by this paper
• A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.

  1993influential reference
• Polymerase chain reaction strategy.

  1992cited by this paper
• A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.

  1992cited by this paper
• Rapid analysis of yeast transformants using colony-PCR.

  1992cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1989cited by this paper
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  1989cited by this paper
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  1987influential reference
• Transformation of intact yeast cells treated with alkali cations.

  1984cited by this paper
• One-step gene disruption in yeast.

  1983cited by this paper
• High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

  1979influential reference
• Isolation and characterisation of a yeast chromosomal replicator

  1979cited by this paper

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