Pancreatic cancer modeling using retrograde viral vector delivery and in vivo CRISPR/Cas9-mediated somatic genome editing

In this study, Chiou et al. describe a novel system for titratable tumor initiation in the adult pancreas through retrograde ductal injection of adenoviral-Cre-expressing and lentiviral-Cre-expressing vectors. They also demonstrate that CRISPR/Cas9-mediated genome editing can be used efficiently in the pancreas in vivo using viral sgRNA/Cre delivery and a Cre-regulated Cas9 mouse allele, providing a new system for putative tumor and metastatic suppressor genes in pancreatic cancer in vivo.

• Publication year

  2015

• Venue

  Genes & Development

• Publication date

  2015-07-15

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1101/gad.264861.115PMID 26178787PMCID 4526740
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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