Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting.

The sorting of transmembrane proteins (e.g., cell surface receptors) into the multivesicular body (MVB) pathway to the lysosomal/vacuolar lumen requires the function of the ESCRT protein complexes. The soluble coiled-coil-containing proteins Vps2, Vps20, Vps24, and Snf7 are recruited from the cytoplasm to endosomal membranes where they oligomerize into a protein complex, ESCRT-III. ESCRT-III contains two functionally distinct subcomplexes. The Vps20-Snf7 subcomplex binds to the endosomal membrane, in part via the myristoyl group of Vps20. The Vps2-Vps24 subcomplex binds to the Vps20-Snf7 complex and thereby serves to recruit additional cofactors to this site of protein sorting. We provide evidence for a role for ESCRT-III in sorting and/or concentration of MVB cargoes.

• Publication year

  2002

• Venue

  Developmental Cell

• Publication date

  2002-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S1534-5807(02)00220-4PMID 12194857
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2001cited by this paper
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  2001cited by this paper
• Sorting in the endosomal system in yeast and animal cells.

  2000cited by this paper
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  2000cited by this paper
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  2000cited by this paper
• The Doa4 deubiquitinating enzyme is required for ubiquitin homeostasis in yeast.

  1999cited by this paper
• Cloning, characterisation, and functional expression of the Mus musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and the yeast VPS4 genes are orthologues and involved in intracellular protein trafficking.

  1999cited by this paper
• The Vps4p AAA ATPase regulates membrane association of a Vps protein complex required for normal endosome function

  1998influential reference
• Endocytosis and intracellular sorting of receptor tyrosine kinases.

  1998cited by this paper
• Fab1p PtdIns(3)P 5-kinase function essential for protein sorting in the multivesicular body.

  1998influential reference
• A Role for Saccharomyces cerevisiae Fatty Acid Activation Protein 4 in Regulating ProteinN-Myristoylation during Entry into Stationary Phase*

  1998cited by this paper
• Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component

  1997cited by this paper
• Ubiquitin‐dependent internalization and down‐regulation of plasma membrane proteins

  1997cited by this paper
• Endosomal transport function in yeast requires a novel AAA‐type ATPase, Vps4p

  1997cited by this paper
• Vps10p cycles between the late-Golgi and prevacuolar compartments in its function as the sorting receptor for multiple yeast vacuolar hydrolases

  1996cited by this paper
• Multilamellar endosome-like compartment accumulates in the yeast vps28 vacuolar protein sorting mutant.

  1996cited by this paper
• Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and then fuse directly with lysosomes

  1996cited by this paper
• The cytoplasmic tail domain of the vacuolar protein sorting receptor Vps10p and a subset of VPS gene products regulate receptor stability, function, and localization.

  1995cited by this paper
• VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae

  1995cited by this paper
• The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene.

  1994cited by this paper
• Multivesicular bodies in HEp-2 cells are maturing endosomes.

  1993cited by this paper
• An MBoC Favorite: Morphological classification of the yeast vacuolar protein-sorting mutants: evidence for a prevacuolar compartment in class E vps mutants

  1992cited by this paper
• Multifunctional yeast high-copy-number shuttle vectors.

  1992cited by this paper
• Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane.

  1992cited by this paper
• Kinase activity controls the sorting of the epidermal growth factor receptor within the multivesicular body.

  1990cited by this paper
• Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases

  1988cited by this paper
• Transformation of intact yeast cells treated with alkali cations.

  1984cited by this paper
• Transformation of intact yeast cells treated with alkali cations

  1983cited by this paper
• Methods in yeast genetics

  1979cited by this paper
• Epidermal growth factor: morphological demonstration of binding, internalization, and lysosomal association in human fibroblasts.

  1978cited by this paper
• Epidermal growth factor

  1972cited by this paper
• Experiments in molecular genetics

  1972cited by this paper

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