Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion

The colocalization of integrins alpha 2 beta 1 and alpha 3 beta 1 at intercellular contact sites of keratinocytes in culture and in epidermis suggests that these integrins may mediate intercellular adhesion (ICA). P1B5, an anti-alpha 3 beta 1 mAb previously reported to inhibit keratinocyte adhesion to epiligrin, was also found to induce ICA. Evidence that P1B5-induced ICA was mediated by alpha 2 beta 1 and alpha 3 beta 1 was obtained using both ICA assays and assays with purified, mAb-immobilized integrins. Selective binding of alpha 2 beta 1-coated beads to epidermal cells or plate-bound alpha 3 beta 1 was observed. This binding was inhibited by mAbs to integrin alpha 3, alpha 2, or beta 1 subunits and could be stimulated by P1B5. We also demonstrate a selective and inhibitable interaction between affinity- purified integrins alpha 2 beta 1 and alpha 3 beta 1. Finally, we show that expression of alpha 2 beta 1 by CHO fibroblasts results in the acquisition of collagen and alpha 3 beta 1 binding. Binding to both of these ligands is inhibited by P1H5, an anti-alpha 2 beta 1 specific mAb. Results of these in vitro experiments suggest that integrins alpha 2 beta 1 and alpha 3 beta 1 can interact and may do so to mediate ICA in vivo. Thus, alpha 3 beta 1 mediates keratinocyte adhesion to epiligrin and plays a second role in ICA via alpha 2 beta 1.

• Publication year

  1993

• Venue

  Journal of Cell Biology

• Publication date

  1993-01-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.120.2.523PMID 8421064PMCID 2119521
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1993cited by this paper
• Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts.

  1992cited by this paper
• Signal transduction by integrins: increased protein tyrosine phosphorylation caused by clustering of beta 1 integrins.

  1991cited by this paper
• Epiligrin, a new cel adhesion ligand for integrin α3β1 in epithelial basement membranes

  1991influential reference
• Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogeneous cytoplasmic domains

  1991cited by this paper
• Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules.

  1991cited by this paper
• Integrin expression during human epidermal development in vivo and in vitro.

  1991cited by this paper
• Cadherin cell adhesion receptors as a morphogenetic regulator.

  1991cited by this paper
• Expression, distribution, and biochemistry of human CD39. Role in activation-associated homotypic adhesion of lymphocytes.

  1991cited by this paper
• The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells

  1990cited by this paper
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  1990cited by this paper
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  1990cited by this paper
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  1990influential reference
• VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site.

  1990cited by this paper
• Changes in keratinocyte adhesion during terminal differentiation: Reduction in fibronectin binding precedes α5β1 integrin loss from the cell surface

  1990cited by this paper
• Adhesion receptors of the immune system

  1990cited by this paper
• A monoclonal antibody to VLA-4 alpha-chain (CDw49d) induces homotypic lymphocyte aggregation.

  1990cited by this paper
• Epidermal differentiation: the bare essentials

  1990influential reference
• Phorbol ester induces increased expression, altered glycosylation, and reduced adhesion of K562 erythroleukemia cell fibronectin receptors.

  1989cited by this paper
• The primary structure of the VLA-2/collagen receptor alpha 2 subunit (platelet GPIa): homology to other integrins and the presence of a possible collagen-binding domain

  1989cited by this paper
• Characterization of primary human keratinocytes transformed by human papillomavirus type 18

  1988cited by this paper
• A monoclonal antibody (NKI-L16) directed against a unique epitope on the alpha-chain of human leukocyte function-associated antigen 1 induces homotypic cell-cell interactions.

  1988cited by this paper
• SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat

  1988cited by this paper
• The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa

  1988cited by this paper
• Epithelial stem cells in vivo

  1988cited by this paper
• Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1).

  1987cited by this paper
• Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits

  1987influential reference
• Integrins: a family of cell surface receptors.

  1987cited by this paper
• Cell migration is essential for sustained growth of keratinocyte colonies: the roles of transforming growth factor-alpha and epidermal growth factor.

  1987cited by this paper
• A-CAM: a 135-kD receptor of intercellular adherens junctions. I. Immunoelectron microscopic localization and biochemical studies

  1986cited by this paper
• A-CAM: a 135-kD receptor of intercellular adherens junctions. II. Antibody-mediated modulation of junction formation

  1986cited by this paper
• Cultivation, frozen storage, and clonal growth of normal human epidermal keratinocytes in serum-free media

  1985cited by this paper
• Epidermal stem cells.

  1983cited by this paper
• Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.

  1975cited by this paper
• Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma.

  1975cited by this paper
• Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell is

  1975cited by this paper
• Structure and function of intercellular junctions.

  1974influential reference

• Cutaneous Innervation in the Red-Eared Slider Turtle: Novel Identification and Analysis of Scale Sensilla and Sensory Corpuscles With Immunofluorescence Insights and Ecological Implications.

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• Laminin 511 E8 fragment promotes to form basement membrane-like structure in human skin equivalents

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• Immunotoxicity and allergenic potential induced by topical application of perfluorooctanoic acid (PFOA) in a murine model.

  2020cites this paper
• Interplay between Cell-Surface Receptors and Extracellular Matrix in Skin

  2020cites this paper
• Mapping transmembrane binding partners for E-cadherin ectodomains

  2020cites this paper
• Deoxycholic acid promotes development of gastroesophageal reflux disease and Barrett's oesophagus by modulating integrin‐αv trafficking

  2017cites this paper
• Histopathological and Immunohistochemical Changes in the Amniotic Membrane of Gestational Diabetic Mothers

  2017cites this paper
• Regulation of Intracellular Trafficking of Laminin Binding Integrins in Prostate Cancer

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• Immunohistochemical distribution of Ki67 in epidermis of thick glabrous skin of human digits

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• Effects of hyperbaric oxygen therapy on rat facial skin

  2016cites this paper
• Global Transcriptome Abnormalities of the Eutopic Endometrium From Women With Adenomyosis

  2016cites this paper
• Integrins as Therapeutic Targets for Respiratory Diseases

  2015cites this paper
• Modulation de l'expression du gène de la sous-unité a5 de l'intégrine a5B1 par les composantes de la matrice extracellulaire durant la cicatrisation de l'épithélium cornéen

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• Interplay between cadherins and α2β1 integrin differentially regulates melanoma cell invasion

  2015cites this paper
• Development of In Vitro Method for Assaying Anti-Angiogenic Effect of Drugs

  2014cites this paper
• Cooperation between integrins and growth factor receptors in signaling and endocytosis.

  2011cites this paper
• Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β

  2010influential citation
• Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy.

  2010cites this paper
• Identification of Integrin α3 as a New Substrate of the Adenovirus E4orf6/E1B 55-Kilodalton E3 Ubiquitin Ligase Complex

  2009cites this paper
• Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in adenomyosis.

  2009cites this paper
• The role of integrins in corneal wound healing.

  2009cites this paper
• Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3.

  2009cites this paper
• Identification of Integrin α 3 as a New Substrate of the Adenovirus E 4 orf 6 / E 1 B 55 K E 3 Ubiquitin Ligase Complex

  2009cites this paper
• Keratinocyte integrins in wound healing and chronic inflammation of the human periodontium.

  2008cites this paper
• The tetraspanin CD9 modulates epidermal growth factor receptor signaling in cancer cells

  2008cites this paper
• Expression of the VLA (31 Integrin Family in Bladder Cancer

  2007cites this paper
• What is the alpha2beta1 integrin doing in the epidermis?

  2007cites this paper
• What Is the α2β1 Integrin Doing in the Epidermis

  2007cites this paper
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  2007cites this paper
• Control of integrin genes expression in the eye.

  2007cites this paper
• Hemopexin domains as multifunctional liganding modules in matrix metalloproteinases and other proteins

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• Stem cell niches

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• Characterization of the promoter for the alpha3 integrin gene in various tumor cell lines: roles of the Ets- and Sp-family of transcription factors.

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• Integrin alpha6beta4-erbB2 complex inhibits haptotaxis by up-regulating E-cadherin cell-cell junctions in keratinocytes.

  2005cites this paper
• Mucous membrane pemphigoid.

  2005cites this paper
• Expression of integrin receptors and ICAM-1 on keratinocytes in vivo and in an in vitro reconstructed epidermis: Effect of sodium dodecyl sulphate

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• Integrin receptors and extracellular matrix components in parotid neoplasms.

  2005cites this paper
• alpha3beta1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development.

  2004cites this paper
• Physiological and pathological roles of alpha3beta1 integrin.

  2004cites this paper
• Laminin 5 deposition regulates keratinocyte polarization and persistent migration

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• Regulation of melanoma cell migration and invasion by laminin-5 and α3β1 integrin (VLA-3)

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  2004cites this paper
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• The role of E‐cadherin in nevogenesis: an experimental study using epidermal reconstructs

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• CD151 regulates epithelial cell–cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization

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• Functions of pulmonary epithelial integrins: from development to disease.

  2003cites this paper
• Alpha 6 beta 4 integrin regulates keratinocyte chemotaxis through differential GTPase activation and antagonism of alpha 3 beta 1 integrin.

  2003cites this paper
• Negative impact of DEP exposure on human airway epithelial cell adhesion, stiffness, and repair.

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• Modification to the approach of the diagnosis of mucous membrane pemphigoid: A case report and literature review.

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• Interaction of integrin receptors with extracellular matrix is involved in trophoblast giant cell migration in bovine placentomes.

  2003cites this paper
• EGF receptor ligands.

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• Differential regulation of cellular adhesion and migration by recombinant laminin‐5 forms with partial deletion or mutation within the G3 domain of α3 chain

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• Characterization of the promoter for the mouse a 3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity

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• Role of the alpha3beta1 and alpha6beta4 integrins in tumor invasion.

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• Characterization of the promoter for the mouse alpha 3 integrin gene.

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• Characterization of the promoter for the mouse α3 integrin gene

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• Implantation-Associated Changes in Bovine Uterine Expression of Integrins and Extracellular Matrix1

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• Regulation of melanoma cell migration and invasion by laminin-5 and alpha3beta1 integrin (VLA-3).

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• Reduced expression of CD9 in oral squamous cell carcinoma: CD9 expression inversely related to high prevalence of lymph node metastasis.

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• The expression of laminin-5 and ultrastructure of the interface between basal cells and underlying stroma in the keratoconus cornea.

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• Activation of beta1 integrins induces cell-cell adhesion.

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• Lymphoid adhesion promotes human thymic epithelial cell survival via NF-(kappa)B activation.

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• Beta(1)-integrins are involved in migration of human fetal tracheal epithelial cells and tubular morphogenesis.

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• Integrin alpha 2 beta 1-dependent EGF receptor activation at cell-cell contact sites.

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• Adhesive properties of human basal epidermal cells: an analysis of keratinocyte stem cells, transit amplifying cells, and postmitotic differentiating cells.

  2000cites this paper
• Cell-cell and cell-substrate adhesion in cultured Drosophila imaginal disc cells

  2000cites this paper
• Markers of bladder cancer State of the art.

  2000cites this paper
• Changing patterns of localization of putative stem cells in developing human hair follicles.

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• Expression of integrin subunits correlates with differentiation of epithelial cell lineages in developing human gastric mucosa

  2000cites this paper
• Integrins as receptors for laminins

  2000cites this paper
• Bi-directional signal transduction by integrin receptors.

  2000cites this paper
• Galectin-8 binding to integrins inhibits cell adhesion and induces apoptosis.

  2000cites this paper
• Differences of alpha3beta1 integrin glycans from different human bladder cell lines.

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• Distinct functions of alpha3 and alpha(v) integrin receptors in neuronal migration and laminar organization of the cerebral cortex.

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• Ultrastructural localization of alpha‐3 integrin subunit in malignant melanoma and adjacent epidermis

  1999cites this paper
• Integrins and human intestinal cell functions.

  1999cites this paper
• Characterization of mouse integrin alpha3 subunit gene.

  1999cites this paper
• Contemporary issues in the diagnosis of oral pemphigoid: a selective review of the literature.

  1999cites this paper
• (Alpha)3(beta)1 integrin regulates epithelial cytoskeletal organization.

  1999cites this paper
• Cyclic modulation of integrin expression in bovine endometrium.

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• Characterization of Mouse Integrin α3 Subunit Gene

  1999cites this paper
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