Biomedical analysis of formalin‐fixed, paraffin‐embedded tissue samples: The Holy Grail for molecular diagnostics

HIGHLIGHTSFormalin fixation with paraffin embedment is most widely used for tissue fixation.Formalin fixation differently affects various biomolecules.Biomarker discovery form FFPE samples is the Holy Grail of molecular diagnostics. ABSTRACT More than a century ago in 1893, a revolutionary idea about fixing biological tissue specimens was introduced by Ferdinand Blum, a German physician. Since then, a plethora of fixation methods have been investigated and used. Formalin fixation with paraffin embedment became the most widely used types of fixation and preservation method, due to its proper architectural conservation of tissue structures and cellular shape. The huge collection of formalin‐fixed, paraffin‐embedded (FFPE) sample archives worldwide holds a large amount of unearthed information about diseases that could be the Holy Grail in contemporary biomarker research utilizing analytical omics based molecular diagnostics. The aim of this review is to critically evaluate the omics options for FFPE tissue sample analysis in the molecular diagnostics field.

• Publication year

  2018

• Venue

  Journal of Pharmaceutical and Biomedical Analysis

• Publication date

  2018-06-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.jpba.2018.03.065PMID 29627729
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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