Electrostatic Stabilization of a Native Protein Structure in the Gas Phase**

Recently, a general picture has been proposed of how long, and to what extent, native protein structure can be retained in the gas phase.1a In particular, molecular dynamics simulations suggest that salt bridges and ionic hydrogen bonds on the protein surface can transiently stabilize the global fold shortly after desolvation.1b However, the use of native mass spectrometry2 for studying protein solution structure is still controversial, mostly because site-specific experimental gas-phase data3 is scarce. Here we report electron capture dissociation (ECD)4 data on the gas-phase structures of the three-helix bundle protein KIX5 (Figure 1) that indicate substantial preservation of the native solution structure on a timescale of at least 4 s. We demonstrate that in the gas phase, the most stable regions are those stabilized by salt bridges and ionic hydrogen bonds.

• Publication year

  2010

• Venue

  Angewandte Chemie

• Publication date

  2010-11-09

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1002/anie.201005112PMID 21246681PMCID 3045662
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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