Identification of Inhibitors of the Association of ZAP-70 with the T Cell Receptor by High-Throughput Screen

ZAP-70 is a critical molecule in the transduction of T cell antigen receptor signaling and the activation of T cells. Upon activation of the T cell antigen receptor, ZAP-70 is recruited to the intracellular ζ-chains of the T cell receptor, where ZAP-70 is activated and colocalized with its substrates. Inhibitors of ZAP-70 could potentially function as treatments for autoimmune diseases or organ transplantation. In this work, we present the design, optimization, and implementation of a screen for inhibitors that would disrupt the interaction between ZAP-70 and the T cell antigen receptor. The screen is based on a fluorescence polarization assay for peptide binding to ZAP-70.

• Publication year

  2017

• Venue

  SLAS discovery : advancing life sciences R & D

• Publication date

  2017-03-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1177/1087057116681407PMID 27932698PMCID PMC5491343
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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