Demonstration by in situ hybridization of dissimilar IL-1 beta gene expression in human alveolar macrophages and blood monocytes in response to lipopolysaccharide.

IL-1 beta is the major form of IL-1 produced by mononuclear phagocytes. To evaluate the possible mechanisms underlying the observation that mature populations of human mononuclear phagocytes as alveolar macrophages are relatively poor IL-1 producers compared with blood monocytes, the expression of the IL-1 beta gene mRNA transcripts was evaluated in LPS-stimulated normal autologous blood monocytes and alveolar macrophages by using a IL-1 beta cDNA probe. Although Northern analysis demonstrated that stimulated monocytes and alveolar macrophages both express 1.8-kb IL-1 beta mRNA transcripts, cytoplasmic dot blot analysis showed that the total IL-1 beta mRNA content in alveolar macrophages was only 38 +/- 5% of that in blood monocytes. In situ hybridization with antisense and sense IL-1 beta RNA probes demonstrated that whereas most of stimulated blood monocytes contained IL-1 beta mRNA transcripts, a significant proportion of autologous alveolar macrophages stimulated in an identical fashion did not express the IL-1 beta gene. Within 4 h after LPS stimulation, IL-1 beta mRNA transcripts were detected in 81 +/- 6% monocytes, whereas only 16 +/- 9% of alveolar macrophages were positive, and by 18 h this had increased only to 43 +/- 15%. Quantification of the size distribution of the IL-1 beta mRNA expressing mononuclear phagocytes demonstrated that, among the population of alveolar macrophages, the cells expressing this gene were not confined to those that were "monocyte-like." These observations demonstrate that there is a heterogeneity among population of mononuclear phagocytes in their ability to express the gene for IL-1 beta, which could explain the differences observed in IL-1 production.

• Publication year

  1988

• Venue

  Journal of Immunology

• Publication date

  1988-06-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.4049/jimmunol.140.11.3822PMID 3259599
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Human alveolar macrophages suppress interleukin-1 (IL-1) activity via the secretion of prostaglandin E2.

  2015cited by this paper
• Evaluation of alveolar macrophages in normals and individuals with active pulmonary sarcoidosis for the spontaneous expression of the interleukin-1 beta gene.

  1987cited by this paper
• Interleukin 1 induces interleukin 1. II. Recombinant human interleukin 1 induces interleukin 1 production by adult human vascular endothelial cells.

  1987cited by this paper
• Expression in Escherichia coli of fully active recombinant human IL 1 beta: comparison with native human IL 1 beta.

  1987cited by this paper
• Interleukin 1 induces interleukin 1. I. Induction of circulating interleukin 1 in rabbits in vivo and in human mononuclear cells in vitro.

  1987cited by this paper
• Spatial patterns of metallothionein mRNA expression in the sea urchin embryo.

  1986cited by this paper
• Human interleukin 1.

  1986cited by this paper
• Human Monocyte Production of Interleukin‐1: Parameters of the Induction of Interleukin‐1 Secretion by Lipopolysaccharides

  1986cited by this paper
• Interleukin-1 production and antigen presentation by normal human peritoneal macrophages.

  1986cited by this paper
• Detection of lymphocytes expressing human T-lymphotropic virus type III in lymph nodes and peripheral blood from infected individuals by in situ hybridization.

  1986cited by this paper
• Immunocytochemical detection of interleukin 1 within stimulated human monocytes

  1986cited by this paper
• Interleukin 1 alpha and interleukin 1 beta bind to the same receptor on T cells.

  1986cited by this paper
• Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line.

  1986cited by this paper
• Human pulmonary macrophages. Functional comparison of cells obtained from whole lung and by bronchoalveolar lavage.

  1986cited by this paper
• Molecular probes for the development and plasticity of neural crest derivatives.

  1985cited by this paper
• Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs

  1985cited by this paper
• Characterization of mononuclear phagocyte subpopulations in the human lung by using monoclonal antibodies: changes in alveolar macrophage phenotype associated with pulmonary sarcoidosis.

  1985cited by this paper
• Human monocyte heterogeneity: Interleukin 1 and prostaglandin E2 production by separate subsets

  1985cited by this paper
• An update on human interleukin-1: From molecular biology to clinical relevance

  1985cited by this paper
• Lack of binding of bacterial lipopolysaccharide to mouse lung macrophages and restoration of binding by gamma interferon

  1985cited by this paper
• The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities.

  1985cited by this paper
• Differential interleukin 1 elaboration by unfractionated and density fractionated human alveolar macrophages and blood monocytes: relationship to cell maturity.

  1985cited by this paper
• Interleukin 1: an immunological perspective.

  1985cited by this paper
• Amino acid sequence analysis of human interleukin 1 (IL-1). Evidence for biochemically distinct forms of IL-1

  1985cited by this paper
• Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.

  1984cited by this paper
• Normal human alveolar macrophages obtained by bronchoalveolar lavage have a limited capacity to release interleukin-1.

  1984cited by this paper
• Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed

  1983cited by this paper
• A simple and very efficient method for generating cDNA libraries.

  1983cited by this paper
• Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.

  1982cited by this paper
• The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte-activating factor produced by rabbit alveolar macrophages.

  1981cited by this paper
• Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

  1980cited by this paper
• Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage.

  1979cited by this paper
• Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

  1978cited by this paper
• The separation, long-term cultivation, and maturation of the human monocyte

  1977cited by this paper
• RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

  1977cited by this paper
• Ribonucleic acid isolated by cesium chloride centrifugation.

  1974cited by this paper

• Expression of SAA1, SAA2 and SAA4 genes in human primary monocytes and monocyte-derived macrophages

  2019cites this paper
• LPS-induced cytokine production in human monocytes and macrophages.

  2011cites this paper
• processing and release of IL-1 Differential requirement for the activation of the inflammasome for

  2009cites this paper
• Differential requirement for the activation of the inflammasome for processing and release of IL-1beta in monocytes and macrophages.

  2009cites this paper
• processing and release of IL-1{beta} in monocytes and macrophages Differential requirement for the activation of the inflammasome for

  2009cites this paper
• The role of NLRs and TLRs in the activation of the inflammasome

  2008cites this paper
• Asbestos-induced Mkp-3 Expression Augments Tnf-a Gene Expression in Human Monocytes

  2008cites this paper
• Asbestos-induced MKP-3 expression augments TNF-alpha gene expression in human monocytes.

  2008cites this paper
• The role of NLRs and TLRs in the activation of the inflammasome.

  2008cites this paper
• Evaluation ofFibronectin Gene Expression by In Situ Hybridization Diferential Expression ofthe Fibronectin Gene Among Populations of Human Alveolar Macrophages

  2007cites this paper
• Induction of interleukin-1 α and β gene transcription in mouse peritoneal exudate cells after intraperitoneal infection with herpes simplex virus-1

  2005cites this paper
• An Inducible Enhancer Controls the Expression of the Human Interleukin 1 $ Gene

  2005cites this paper
• High levels of catalase and glutathione peroxidase activity dampen H2O2 signaling in human alveolar macrophages.

  2004cites this paper
• Upregulation of bronchioloalveolar carcinoma-derived C-X-C chemokines by tumor infiltrating inflammatory cells

  2003cites this paper
• Human Neutrophils Express the al-Antitrypsin Gene and Produce alAn ti trypsin

  2003cites this paper
• EXPRESSION OF THE NEUTROPHIL ELASTASE GENE DURING HUMAN BONE MARROW CELL DIFFERENTIATION

  2003cites this paper
• Fibrinogen cooperates with cytokines to induce interleukin-6 receptor mRNA expression in human hematopoietic CD34+ progenitors.

  2001cites this paper
• p21(cip1) mRNA is controlled by endogenous transforming growth factor-beta1 in quiescent human hematopoietic stem/progenitor cells.

  2000cites this paper
• Differential gene regulation of interleukin-1 ligands and receptors in bovine peripheral blood neutrophils and mononuclear cells in response to E. coli lipopolysaccharide (LPS).

  2000cites this paper
• The high proliferative potential-quiescent (HPP-Q) cell assay allows an optimized evaluation of gene transfer efficiency into primitive hematopoietic stem/progenitor cells

  2000cites this paper
• p21cip1 mRNA is controlled by endogenous transforming growth factor‐β1 in quiescent human hematopoietic stem/progenitor cells

  2000cites this paper
• Induction of Interleukin 8 (IL‐8) Production by Pseudomonas Nitrite Reductase in Human Alveolar Macrophages and Epithelial Cells

  1999cites this paper
• Pseudomonas Nitrite Reductase in Human Alveolar Macrophages and Epithelial Cells

  1999cites this paper
• Regulation of interleukin-12 production in human cells stimulated with Mycobacterium bovis BCG.

  1998cites this paper
• Effects of phosphodiesterase inhibitors on secretions of human monokines.

  1998cites this paper
• Induction of gamma interferon production in human alveolar macrophages by Mycobacterium tuberculosis

  1997cites this paper
• The role of alveolar macrophages and dendritic cells in allergic airway sensitization

  1996cites this paper
• Regulation of human alveolar macrophage inflammatory cytokine production by interleukin-10.

  1996cites this paper
• IL-3, GM-CSF and CSF-1 modulate c-fms mRNA more rapidly in human early monocytic progenitors than in mature or transformed monocytic cells.

  1996cites this paper
• Human alveolar macrophages produce predominantly the 35-kD pro-forms of interleukin-1 alpha and interleukin-1 beta when stimulated with lipopolysaccharide.

  1995cites this paper
• Expression of a 2.8-kb PDGF-B/c-sis transcript and synthesis of PDGF-like protein by human lung fibroblasts.

  1995cites this paper
• EMERGENCE OF INFLAMMATORY ALVEOLAR MACROPHAGES DURING REJECTION OR INFECTION AFTER LUNG TRANSPLANTATION

  1994cites this paper
• Functional profile of tissue-infiltrating and circulating CD68+ cells in giant cell arteritis. Evidence for two components of the disease.

  1994cites this paper
• HIV-1 in human alveolar macrophages from infected patients is latent in vivo but replicates after in vitro stimulation.

  1994cites this paper
• Gene Transfer to Freshly Isolated Human Respiratory Epithelial Cells In Vitro Using a Replication-Deficient Adenovirus Containing the Human Cystic Fibrosis Transmembrane Conductance Regulator cDNA

  1994cites this paper
• Application of fine-needle aspiration to the demonstration of ERBB2 and MYC expression by in situ hybridization in breast carcinoma.

  1994cites this paper
• Alveolar macrophages from patients with beryllium disease and sarcoidosis express increased levels of mRNA for tumor necrosis factor-alpha and interleukin-6 but not interleukin-1 beta.

  1994cites this paper
• Production of interleukin 6 by granulomas of giant cell arteritis.

  1994cites this paper
• Expression of Tumor Necrosis Factor-α Gene in Alveolar Macrophages from Patients with the Adult Respiratory Distress Syndrome

  1993cites this paper
• Evidence that granulocyte macrophage-colony-stimulating factor regulates the distribution and differentiated state of dendritic cells/Langerhans cells in human lung and lung cancers.

  1993cites this paper
• CSF‐1 control of C‐FMS expression in normal human bone marrow progenitors

  1993cites this paper
• Expression of tumor necrosis factor-alpha gene in alveolar macrophages from patients with the adult respiratory distress syndrome.

  1993cites this paper
• Mechanisms generating functionally heterogeneous macrophages: chaos revisited

  1993cites this paper
• The Interaction of Interleukin-1 With the Actors of Asthma

  1993cites this paper
• In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium.

  1992cites this paper
• In situ hybridization of the mRNA for interferon-gamma, interferon-alpha E, interferon-beta, interleukin-1 beta and interleukin-6 and characterization of infiltrating cells in thyroid tissues.

  1992cites this paper
• Cytokines in symptomatic asthma airways.

  1992cites this paper
• Cytokines of the lung.

  1992cites this paper
• Synthetic surfactant (Exosurf) inhibits endotoxin-stimulated cytokine secretion by human alveolar macrophages.

  1992cites this paper
• Review: transcriptional and post-transcriptional regulation of interleukin 1 gene expression.

  1992cites this paper
• Evidence by in situ hybridization that c-erbB-2 proto-oncogene expression is a marker of malignancy and is expressed in lung adenocarcinomas.

  1992cites this paper
• Macrophage and monocyte IL-1 beta regulation differs at multiple sites. Messenger RNA expression, translation, and post-translational processing.

  1992cites this paper
• In situ hybridization of the mRNA for interferon-γ, interferon-αE, interferon-β, interleukin-1β and interleukin-6 and characterization of infiltrating cells in thyroid tissues

  1992cites this paper
• Processing of interleukin-1 in cells of monocytic lineage is differentiation-dependent.

  1992cites this paper
• T-lymphocytes that accumulate in the lung in sarcoidosis have evidence of recent stimulation of the T-cell antigen receptor.

  1992cites this paper
• Cloning, expression and characterization of ovine interleukins 1 alpha and beta.

  1992cites this paper
• Cloning, expression and characterization of ovine interleukins 1α and β

  1992cites this paper
• Secretion by Human Alveolar Macrophages

  1992cites this paper
• c-fos mRNA constitutive expression by mature human megakaryocytes.

  1992cites this paper
• Evaluation of the expression of the cationic peptide gene in various types of leukocytes

  1992cites this paper
• Adenovirus–mediated in vivo gene transfer and expression in normal rat liver

  1992cites this paper
• Lipopolysaccharide binding protein enhances the responsiveness of alveolar macrophages to bacterial lipopolysaccharide. Implications for cytokine production in normal and injured lungs.

  1992cites this paper
• In Situ Hybridization of Interferon‐Gamma Producing Peripheral Blood Mononuclear Cells

  1991cites this paper
• Activated Macrophage Conditioned Medium: Identification of the Soluble Factors Inducing Cytotoxicity and the L‐Arginine Dependent Effector Mechanism

  1991cites this paper
• Induction of interleukin-1 beta mRNA in adult rat brain.

  1991cites this paper
• Interleukin-8 gene expression from human alveolar macrophages: the role of adherence.

  1991cites this paper
• Pasteurella haemolytica A1 purified capsular polysaccharide does not stimulate interleukin-1 and tumor necrosis factor release by bovine monocytes and alveolar macrophages.

  1991cites this paper
• Macrophage heterogeneity occurs through a developmental mechanism.

  1991cites this paper
• Recombinant interleukin-1 beta inhibits elastin formation by a neonatal rat lung fibroblast subtype.

  1991cites this paper
• Recent advances in the immunology of interstitial lung disease.

  1991cites this paper
• Substance P does not alter interleukin-1 expression by splenic or granuloma macrophages in murine schistosomiasis.

  1991cites this paper
• Immunocytochemical analysis of interferon-1β production by human monocytes

  1991cites this paper
• Human neutrophils express the alpha 1-antitrypsin gene and produce alpha 1-antitrypsin.

  1991cites this paper
• Applications of in situ hybridization.

  1991cites this paper
• Recent advances in the immunology of interstitial lung disease

  1991cites this paper
• Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo.

  1991cites this paper
• Involvement of Epidermal and Lymph Node Dendritic Cells in Skin HSV-1 Infection

  1991cites this paper
• Induction of interleukin-1β mRNA in adult rat brain

  1991cites this paper
• Increased expression of the interleukin-8 gene by alveolar macrophages in idiopathic pulmonary fibrosis. A potential mechanism for the recruitment and activation of neutrophils in lung fibrosis.

  1991cites this paper
• Demonstration of interleukin-1 beta transcripts in acute myeloblastic leukemic cells by in situ hybridization.

  1990cites this paper
• Lipopolysaccharide-induced production of cytokines by bone marrow-derived macrophages: dissociation between intracellular interleukin 1 production and interleukin 1 release.

  1990cites this paper
• An inducible enhancer controls the expression of the human interleukin 1 beta gene.

  1990cites this paper
• Signals involved in interleukin 1 synthesis and release by lipopolysaccharide-stimulated monocytes/macrophages.

  1990cites this paper
• The production of alveolar macrophage-derived growth-regulating proteins in response to lung injury.

  1990cites this paper
• Localization by in situ hybridization of gamma-glutamyl transpeptidase mRNA in the rat kidney using 35S-labeled RNA probes.

  1990cites this paper
• Analysis of IL-1 and TNF-alpha gene expression in human rheumatoid synoviocytes and normal monocytes by in situ hybridization.

  1990cites this paper
• Induction of interleukin-1 alpha and beta gene transcription in mouse peritoneal exudate cells after intraperitoneal infection with herpes simplex virus-1.

  1990cites this paper
• Hyporesponsiveness of human alveolar leukocytes to interferon-alpha and interferon-gamma inducers.

  1990cites this paper
• Secretion of interleukin-1 beta by a leukemia cell line in response to lipopolysaccharide and mezerein.

  1990cites this paper
• Human alveolar macrophage and blood monocyte interleukin-6 production.

  1990cites this paper
• Rat tracheal epithelial cells produce granulocyte/macrophage colony-stimulating factor.

  1990cites this paper
• Increased elastase secretion by peripheral blood monocytes in cystic fibrosis patients

  1990cites this paper
• Inflammation in organic dust-induced lung disease: new approaches for research into underlying mechanisms.

  1990cites this paper
• Tissue-specific expression of two gamma-glutamyl transpeptidase mRNAs with alternative 5' ends encoded by a single copy gene in the rat.

  1990cites this paper
• Interleukin-l in the Acute Phase Response

  1989cites this paper
• Expression of the neutrophil elastase gene during human bone marrow cell differentiation

  1989cites this paper
• IL-1 beta production in cultured human monocytes is regulated at multiple levels.

  1989cites this paper
• Interleukin-1 and interleukin-1 inhibitor production by human adherent cells stimulated with periodontopathic bacteria.

  1989cites this paper
• Interleukin-1 in the Acute Phase Response

  1989cites this paper
• Differential regulation of lipopolysaccharide‐induced interleukin 1 and tumor necrosis factor synthesis: effects of endogenous and exogenous glucocorticoids and the role of the pituitary‐adrenal axis

  1989cites this paper