Cbfa1 Is a Positive Regulatory Factor in Chondrocyte Maturation*

Cbfa1 is a transcription factor that belongs to the runt domain gene family. Cbfa1-deficient mice showed a complete lack of bone formation due to the maturational arrest of osteoblasts, demonstrating that Cbfa1 is an essential factor for osteoblast differentiation. Further, chondrocyte maturation was severely disturbed in Cbfa1-deficient mice. In this study, we examined the possibility that Cbfa1 is also involved in the regulation of chondrocyte differentiation. mRNAs for both Cbfa1 isotypes, type I Cbfa1 (Pebp2αA/Cbfa1) and type II Cbfa1 (Osf2/Cbfa1 or til-1), which are different in N-terminal domain, were expressed in terminal hypertrophic chondrocytes as well as osteoblasts. In addition, mRNA for type I Cbfa1 was expressed in other hypertrophic chondrocytes and prehypertrophic chondropcytes. In a chondrogenic cell line, ATDC5, the expression of type I Cbfa1 was elevated prior to differentiation to the hypertrophic phenotype, which is characterized by type X collagen expression. Treatment with antisense oligonucleotides for type I Cbfa1 severely reduced type X collagen expression in ATDC5 cells. Retrovirally forced expression of either type I or type II Cbfa1 in chick immature chondrocytes induced type X collagen and MMP13 expression, alkaline phosphatase activity, and extensive cartilage-matrix mineralization. These results indicate that Cbfa1 is an important regulatory factor in chondrocyte maturation.

• Publication year

  2000

• Venue

  Journal of Biological Chemistry

• Publication date

  2000-03-24

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.275.12.8695PMID 10722711
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cbfa1 Is a Positive Regulatory Factor in Chondrocyte Maturation

  2001cited by this paper
• Induction of matrix metalloproteinases and collagenolysis in chick embryonic membranes before hatching.

  1999cited by this paper
• Regulation of chondrocyte differentiation by Cbfa1.

  1999cited by this paper
• Cbfa1 Isoforms Exert Functional Differences in Osteoblast Differentiation*

  1999cited by this paper
• The physiological role of sterol regulatory element-binding protein-2 in cultured human cells.

  1999cited by this paper
• Collagenase 3 Is a Target of Cbfa1, a Transcription Factor of the runt Gene Family Involved in Bone Formation

  1999cited by this paper
• Maturational disturbance of chondrocytes in Cbfa1‐deficient mice

  1999cited by this paper
• Two Domains Unique to Osteoblast-Specific Transcription Factor Osf2/Cbfa1 Contribute to Its Transactivation Function and Its Inability To Heterodimerize with Cbfβ

  1998cited by this paper
• Cbfa1 in bone development.

  1998cited by this paper
• Transcriptional regulation of osteopontin gene in vivo by PEBP2αA/CBFA1 and ETS1 in the skeletal tissues

  1998cited by this paper
• Expression of the PEBP2alphaA/AML3/CBFA1 gene is regulated by BMP4/7 heterodimer and its overexpression suppresses type I collagen and osteocalcin gene expression in osteoblastic and nonosteoblastic mesenchymal cells.

  1998cited by this paper
• Transcriptional regulation of osteopontin gene in vivo by PEBP2alphaA/CBFA1 and ETS1 in the skeletal tissues.

  1998cited by this paper
• Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts.

  1997cited by this paper
• Runt homology domain proteins in osteoblast differentiation: AML3/CBFA1 is a major component of a bone‐specific complex

  1997cited by this paper
• Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development.

  1997cited by this paper
• Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia.

  1997cited by this paper
• Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation.

  1997cited by this paper
• Cellular Hypertrophy and Calcification of Embryonal Carcinoma‐Derived Chondrogenic Cell Line ATDC5 In Vitro

  1997cited by this paper
• Missense mutations abolishing DNA binding of the osteoblast-specific transcription factor OSF2/CBFA1 in cleidocranial dysplasia

  1997cited by this paper
• Proviral insertions induce the expression of bone-specific isoforms of PEBP2alphaA (CBFA1): evidence for a new myc collaborating oncogene.

  1997cited by this paper
• 1 alpha,25-dihydroxyvitamin D3 inhibits cell growth and chondrogenesis of a clonal mouse EC cell line, ATDC5.

  1996cited by this paper
• Interpreting cDNA sequences: Some insights from studies on translation

  1996cited by this paper
• 1α,25‐dihydroxyvitamin D3 inhibits cell growth and chondrogenesis of a clonal mouse EC cell line, ATDC5

  1996cited by this paper
• Characterization of structural sequences in the chicken osteocalcin gene: Expression of osteocalcin by maturing osteoblasts and by hypertrophic chondrocytes in vitro

  1995cited by this paper
• Subcellular localization of the alpha and beta subunits of the acute myeloid leukemia-linked transcription factor PEBP2/CBF

  1995cited by this paper
• PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene.

  1993cited by this paper
• Responsiveness to retinoic acid changes during chondrocyte maturation.

  1993cited by this paper
• Expression and role of c-myc in chondrocytes undergoing endochondral ossification.

  1993cited by this paper
• A rapid and versatile method to synthesize internal standards for competitive PCR.

  1993cited by this paper
• Method for detecting the expression of bone matrix protein by in situ hybridization using decalcified mineralized tissue

  1993cited by this paper
• Characterization of the mouse type X collagen gene.

  1993cited by this paper
• A chondrogenic cell line derived from a differentiating culture of AT805 teratocarcinoma cells.

  1990cited by this paper
• The Drosophila segmentation gene runt encodes a novel nuclear regulatory protein that is also expressed in the developing nervous system.

  1990cited by this paper
• Type X collagen gene expression is transiently up-regulated by retinoic acid treatment in chick chondrocyte cultures.

  1990cited by this paper
• Cartilage contains mixed fibrils of collagen types II, IX, and XI

  1989cited by this paper
• Ascorbic acid induces alkaline phosphatase, type X collagen, and calcium deposition in cultured chick chondrocytes.

  1989cited by this paper
• Cartilage type IX collagen-proteoglycan contains a large amino-terminal globular domain encoded by multiple exons.

  1988cited by this paper
• The calcification of cartilage matrix in chondrocyte culture: studies of the C-propeptide of type II collagen (chondrocalcin)

  1987cited by this paper
• Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors

  1987cited by this paper
• Collagen gene expression during limb cartilage differentiation

  1986cited by this paper
• A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.

  1982cited by this paper
• A rapid in situ deoxyribonucleic acid assay for determining cell number in culture and tissue.

  1982cited by this paper
• A Simple and Sensitive Histochemical Method for Calcium

  1952cited by this paper

• Osteochondrosis in horses: An overview of genetic and other factors

  2025cites this paper
• RUNX2 is essential for maintaining synchondrosis chondrocytes and cranial base growth

  2025cites this paper
• An Osteoblast-Specific Enhancer and Subenhancer Cooperatively Regulate Runx2 Expression in Chondrocytes

  2025cites this paper
• Chondrocyte maturation bridges two cross-inhibitory subnetworks of the skeletal cell gene regulatory network.

  2025cites this paper
• Runx2 and Polycystins in Bone Mechanotransduction: Challenges for Therapeutic Opportunities

  2024cites this paper
• A Sendai virus-based expression system directs efficient induction of chondrocytes by transcription factor-mediated reprogramming

  2024cites this paper
• Collagen type X expression and chondrocyte hypertrophic differentiation during OA and OS development.

  2024cites this paper
• Bone development by Hedgehog and Wnt signaling, Runx2, and Sp7

  2024cites this paper
• Ddx5 participates in regulation of Col10a1 expression and chondrocyte hypertrophic differentiation in vitro.

  2024cites this paper
• Inhibition of HOXD11 promotes cartilage degradation and induces osteoarthritis development

  2024cites this paper
• Extremely low frequency–electromagnetic fields promote chondrogenic differentiation of adipose-derived mesenchymal stem cells through a conventional genetic program

  2024cites this paper
• Molecular mechanism of miR-203a targeting Runx2 to regulate thiram induced-chondrocyte development.

  2024cites this paper
• Effects of Germanium embedded fabric on the chondrogenic differentiation of adipose derived stem cells.

  2024cites this paper
• Regulation of Skeletal Development and Maintenance by Runx2 and Sp7

  2024cites this paper
• Effects of Different Surface Functionalizations of Silica Nanoparticles on Mesenchymal Stem Cells.

  2024cites this paper
• Emerging biological functions of Twist1 in cell differentiation

  2024cites this paper
• Identification of a second genetic alteration in patients with SHOX deficiency individuals: a potential explanation for phenotype variability.

  2023cites this paper
• Hypertrophic chondrocytes at the junction of musculoskeletal structures

  2023cites this paper
• Multiparity and Aging Impact Chondrogenic and Osteogenic Potential at Symphyseal Enthesis: New Insights into Interpubic Joint Remodeling

  2023cites this paper
• Autonomic nervous regulation of cellular processes during subchondral bone remodeling in osteoarthritis.

  2023cites this paper
• Osteoarthritis: pathogenic signaling pathways and therapeutic targets

  2023cites this paper
• Overexpression of transcription factor FoxA2 in the developing skeleton causes an enlargement of the cartilage hypertrophic zone, but it does not trigger ectopic differentiation in immature chondrocytes.

  2022cites this paper
• Effects of Separate and Combined Exposure of Cadmium and Lead on the Endochondral Ossification in Bufo gargarizans

  2022cites this paper
• Downregulation of miR-30b-5p Facilitates Chondrocyte Hypertrophy and Apoptosis via Targeting Runx2 in Steroid-Induced Osteonecrosis of the Femoral Head

  2022cites this paper
• Hypoplasia of medial pterygoid process in sphenoid bone relates to decreased mesenchymal cell proliferation in the Runx2-haploinsufficient cleidocranial dysplasia mouse model.

  2022cites this paper
• Multiple roles of Runt-related transcription factor-2 in tooth eruption: bone formation and resorption.

  2022cites this paper
• DLX Genes in the Development and Maintenance of the Vertebrate Skeleton: Implications for Human Pathologies

  2022cites this paper
• Effects of Recombinant Adeno-Associated Virus-Mediated Overexpression of Bone Morphogenetic Protein 3 on the Chondrogenic Fate of Human Bone Marrow-Derived Mesenchymal Stromal Cells

  2022cites this paper
• Identification of a familial cleidocranial dysplasia with a novel RUNX2 mutation and establishment of patient-derived induced pluripotent stem cells

  2021cites this paper
• The hypertrophic chondrocyte: To be or not to be.

  2021cites this paper
• RUNX2 regulates leukemic cell metabolism and chemotaxis in high-risk T cell acute lymphoblastic leukemia.

  2021cites this paper
• Dmrt2 promotes transition of endochondral bone formation by linking Sox9 and Runx2

  2021cites this paper
• miR-140-3p regulates the osteogenic differentiation ability of bone marrow mesenchymal stem cells by targeting spred2-mediated autophagy

  2021cites this paper
• Dmrt2 promotes transition of endochondral bone formation by linking Sox9 and Runx2

  2021cites this paper
• Runt‐related transcription factor‐2 (Runx2) is required for bone matrix protein gene expression in committed osteoblasts in mice

  2021cites this paper
• Single-cell rna seq analysis identifies the biomarkers and differentiation of chondrocyte in human osteoarthritis.

  2020cites this paper
• In-silico Identification of Novel Drug Target for Osteoarthritisinhuman using System Network Biology Approaches

  2020cites this paper
• Expression of a Constitutively Active Form of Hck in Chondrocytes Activates Wnt and Hedgehog Signaling Pathways, and Induces Chondrocyte Proliferation in Mice

  2020cites this paper
• Evolutionary repression of chondrogenic genes in the vertebrate osteoblast

  2020influential citation
• Astragaloside accelerates fracture healing via modulating miR-122/p53 and miR-221/RUNX2 signaling pathways

  2020cites this paper
• Molecular Mechanism of Runx2-Dependent Bone Development

  2020cites this paper
• Human pluripotent stem cell-derived chondroprogenitors for cartilage tissue engineering

  2020cites this paper
• Role of thyroid hormones in craniofacial development

  2020cites this paper
• Significance of MEF2C and RUNX3 Regulation for Endochondral Differentiation of Human Mesenchymal Progenitor Cells

  2020cites this paper
• Tissue Engineering of Cartilage Using a Random Positioning Machine

  2020cites this paper
• Species-specific sensitivity to TGFβ signaling and changes to the Mmp13 promoter underlie avian jaw development and evolution

  2020cites this paper
• Runx2 is essential for the transdifferentiation of chondrocytes into osteoblasts

  2020cites this paper
• New Insights Into Cranial Synchondrosis Development: A Mini Review

  2020cites this paper
• TBX1 Regulates Chondrocyte Maturation in the Spheno-occipital Synchondrosis

  2020cites this paper
• Lineage‐specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1‐RUNX2 association in neural crest‐derived osteoprogenitors

  2020cites this paper
• Runx2 silencing promotes adipogenesis via down‐regulation of DLK1 in chondrogenic differentiating MSCs

  2020cites this paper
• Overview of Skeletal Development.

  2020cites this paper
• Antxr1, Which is a Target of Runx2, Regulates Chondrocyte Proliferation and Apoptosis

  2020cites this paper
• Regulation der Chondrozytendifferenzierung durch Atoh8

  2019cites this paper
• Maintenance of SOX9 stability and ECM homeostasis by selenium-sensitive PRMT5 in cartilage.

  2019cites this paper
• Differentiation potential of mesenchymal stem cells through electrical stimulation

  2019cites this paper
• The role of chondroitin sulfate in regulating hypertrophy during MSC chondrogenesis in a cartilage mimetic hydrogel under dynamic loading.

  2019cites this paper
• The osteoblast lineage

  2019cites this paper
• TGFβ/BMP Signaling Pathway in Cartilage Homeostasis

  2019cites this paper
• Epigenetic mechanisms underlying the pathogenesis of osteoarthritis and their clinical relevance

  2019cites this paper
• Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx2

  2019cites this paper
• RUNX transcription factors: orchestrators of development

  2019cites this paper
• Biology of Bone and Cartilage

  2018cites this paper
• 13 Genetic Mouse Models for Osteoarthritis Research

  2018cites this paper
• Smad4 deficiency impairs chondrocyte hypertrophy via the Runx2 transcription factor in mouse skeletal development

  2018cites this paper
• Runx2, an inducer of osteoblast and chondrocyte differentiation

  2018cites this paper
• Layered biomimetic nanocomposites replicate bone surface in three-dimensional cell cultures

  2018cites this paper
• Runx2, an inducer of osteoblast and chondrocyte differentiation

  2018cites this paper
• Runx2 mediated Induction of Novel Targets ST2 and Runx3 Leads to Cooperative Regulation of Hypertrophic Differentiation in ATDC5 Chondrocytes

  2017cites this paper
• DNA methylation of the RUNX2 P1 promoter mediates MMP13 transcription in chondrocytes

  2017cites this paper
• Technology Open Access book

  2017cites this paper
• Cartilage‐Specific and Cre‐Dependent Nkx3.2 Overexpression In Vivo Causes Skeletal Dwarfism by Delaying Cartilage Hypertrophy

  2017cites this paper
• Kruppel-like factor 4 regulates matrix metalloproteinase and aggrecanase gene expression in chondrocytes

  2017cites this paper
• Engineered cartilage regeneration from adipose tissue derived‐mesenchymal stem cells: A morphomolecular study on osteoblast, chondrocyte and apoptosis evaluation

  2017cites this paper
• Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

  2017cites this paper
• Control of the Osteoblast Lineage by Mitogen-Activated Protein Kinase Signaling

  2017cites this paper
• Cellular senescence in osteoarthritis pathology

  2017cites this paper
• Roles of Runx2 in Skeletal Development.

  2017cites this paper
• The differentially DNA-methylated region responsible for expression of runt-related transcription factor 2

  2016cites this paper
• Continuous hydrostatic pressure induces differentiation phenomena in chondrocytes mediated by changes in polycystins, SOX9, and RUNX2

  2016cites this paper
• Editorial: Evolution of Organismal Form: From Regulatory Interactions to Developmental Processes and Biological Patterns

  2016cites this paper
• Modélisation du syndrome d'Andersen dans les cellules souches pluripotentes induites : implication du canal potassique Kir2.1 dans la morphogenèse osseuse

  2016cites this paper
• Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13

  2016cites this paper
• Cleidocranial dysplasia and RUNX2‐clinical phenotype–genotype correlation

  2016cites this paper
• A combined series of Fgf9 and Fgf18 mutant alleles identifies unique and redundant roles in skeletal development.

  2016cites this paper
• Expression of TGFβ-family signalling components in ageing cartilage: age-related loss of TGFβ and BMP receptors.

  2016cites this paper
• Cartilage Regeneration Using Pluripotent Stem Cell‐Derived Chondroprogenitors: Promise and Challenges

  2016cites this paper
• Novel Genetic Variants for Cartilage Thickness and Hip Osteoarthritis

  2016cites this paper
• Correlation between Gene Expression and Osteoarthritis Progression in Human

  2016cites this paper
• MiR-690, a Runx2-targeted miRNA, regulates osteogenic differentiation of C2C12 myogenic progenitor cells by targeting NF-kappaB p65

  2016cites this paper
• Overexpression of RBP 4 promotes proliferation , differentiation and mineralization of MC 3 T 3E 1

  2016cites this paper
• The microRNA-23a has limited roles in bone formation and homeostasis in vivo.

  2015cites this paper
• A pathway to bone: signaling molecules and transcription factors involved in chondrocyte development and maturation

  2015cites this paper
• Runx2: Structure, function, and phosphorylation in osteoblast differentiation.

  2015cites this paper
• Chapter 28 – Skeletal Development

  2015cites this paper
• Enhancement of Runx2 Expression Is Potentially Linked to β‐Catenin Accumulation in Canine Intervertebral Disc Degeneration

  2015cites this paper
• Mutant activated FGFR3 impairs endochondral bone growth by preventing SOX9 downregulation in differentiating chondrocytes.

  2015cites this paper
• The functions of Runx family transcription factors and Cbfb in skeletal development

  2015cites this paper
• Effect of Fibroblast Growth Factor 2 on Equine Synovial Fluid Chondroprogenitor Expansion and Chondrogenesis

  2015cites this paper
• Cell-based articular cartilage repair: the link between development and regeneration.

  2015cites this paper