Sexual attraction in the silkworm moth: structure of the pheromone-binding-protein-bombykol complex.

BACKGROUND Insects use volatile organic molecules to communicate messages with remarkable sensitivity and specificity. In one of the most studied systems, female silkworm moths (Bombyx mori) attract male mates with the pheromone bombykol, a volatile 16-carbon alcohol. In the male moth's antennae, a pheromone-binding protein conveys bombykol to a membrane-bound receptor on a nerve cell. The structure of the pheromone-binding protein, its binding and recognition of bombykol, and its full role in signal transduction are not known. RESULTS The three-dimensional structure of the B. mori pheromone-binding protein with bound bombykol has been determined by X-ray diffraction at 1.8 A resolution. CONCLUSIONS The pheromone binding protein of B. mori has six helices, and bombykol binds in a completely enclosed hydrophobic cavity formed by four antiparallel helices. Bombykol is bound in this cavity through numerous hydrophobic interactions, and sequence alignments suggest critical residues for specific pheromone binding.

• Publication year

  2000

• Venue

  Chemistry and Biology

• Publication date

  2000-02-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/S1074-5521(00)00078-8PMID 10662696
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Recombinant pheromone binding protein 1 from Mamestra brassicae (MbraPBP1). Functional and structural characterization.

  1999cited by this paper
• Disulfide structure of the pheromone binding protein from the silkworm moth, Bombyx mori

  1999cited by this paper
• A new class of hexahelical insect proteins revealed as putative carriers of small hydrophobic ligands.

  1999influential reference
• Efficient anisotropic refinement of macromolecular structures using FFT.

  1999influential reference
• Olfactory reception in invertebrates.

  1999influential reference
• Conformational Change in the Pheromone-binding Protein fromBombyx mori Induced by pH and by Interaction with Membranes*

  1999influential reference
• Crystallography & NMR system: A new software suite for macromolecular structure determination.

  1998cited by this paper
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  1998influential reference
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  1998cited by this paper
• An Algorithm for Automatic Indexing of Oscillation Images using Fourier Analysis

  1997cited by this paper
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  1997cited by this paper
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  1997cited by this paper
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  1997cited by this paper
• [27] Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods.

  1997cited by this paper
• Binding proteins from the antennae of Bombyx mori.

  1996cited by this paper
• Odorant-binding proteins in insects.

  1995influential reference
• Protein structure encodes the ligand binding specificity in pheromone binding proteins.

  1995cited by this paper
• The CCP4 suite: programs for protein crystallography.

  1994cited by this paper
• Recombinant Proteins Can Be Isolated from E. coli Cells by Repeated Cycles of Freezing and Thawing

  1994cited by this paper
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  1993cited by this paper
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  1993cited by this paper
• ALSCRIPT: a tool to format multiple sequence alignments.

  1993cited by this paper
• Molecular data processing

  1992cited by this paper
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  1992cited by this paper
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  1991cited by this paper
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  1991cited by this paper
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  1991cited by this paper
• WHAT IF: a molecular modeling and drug design program.

  1990cited by this paper
• Multiple sequence alignment with hierarchical clustering.

  1988cited by this paper
• Techniques in Pheromone Research

  1984cited by this paper
• Bombykol revisited: reflections on a pioneering period and on some of its consequences.

  1984cited by this paper
• Pheromone binding and inactivation by moth antennae

  1981cited by this paper
• [Smell threshold of the silkworm].

  1970cited by this paper
• Uber den sexsual-lockstoff des seidenspinners Bombyx mori

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