Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions

Abstract Once materials come into contact with a biological fluid containing proteins, proteins are generally—whether desired or not—attracted by the material's surface and adsorb onto it. The aim of this Review is to give an overview of the most commonly used characterization methods employed to gain a better understanding of the adsorption processes on either planar or curved surfaces. We continue to illustrate the benefit of combining different methods to different surface geometries of the material. The thus obtained insight ideally paves the way for engineering functional materials that interact with proteins in a predetermined manner.

• Publication year

  2018

• Venue

  Angewandte Chemie

• Publication date

  2018-08-30

• Fields of study

  Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.1002/anie.201712448PMID 29663610PMCID 6391961
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Theranostic nanomedicine.

  2020cited by this paper
• Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions

  2018cited by this paper
• Hydrophilie als bestimmender Faktor des Stealth‐Effekts von Polyphosphoester‐funktionalisierten Nanoträgern

  2018cited by this paper
• Dynamic blue light-switchable protein patterns on giant unilamellar vesicles.

  2018cited by this paper
• Hydrophilicity Regulates the Stealth Properties of Polyphosphoester-Coated Nanocarriers.

  2018cited by this paper
• Protein corona composition of poly(ethylene glycol)- and poly(phosphoester)-coated nanoparticles correlates strongly with the amino acid composition of the protein surface.

  2017cited by this paper
• Determination of Absolute Orientation of Protein α-Helices at Interfaces Using Phase-Resolved Sum Frequency Generation Spectroscopy.

  2017cited by this paper
• 3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics

  2017cited by this paper
• Mitochondria Targeted Protein-Ruthenium Photosensitizer for Efficient Photodynamic Applications

  2017cited by this paper
• Methods of protein corona isolation for magnetic nanoparticles.

  2017cited by this paper
• Experimental separation steps influence the protein content of corona around mesoporous silica nanoparticles.

  2017cited by this paper
• LK peptide side chain dynamics at interfaces are independent of secondary structure.

  2017cited by this paper
• The timeline of corona formation around silica nanocarriers highlights the role of the protein interactome.

  2017cited by this paper
• Biomedical surface analysis: Evolution and future directions (Review)

  2017cited by this paper
• A Mini Review of the Key Components used for the Development of High-Speed Atomic Force Microscopy

  2017cited by this paper
• Strategies in relative and absolute quantitative mass spectrometry based proteomics

  2017cited by this paper
• ToF-SIMS and XPS Characterization of Protein Films Adsorbed onto Bare and Sodium Styrenesulfonate-Grafted Gold Substrates.

  2016cited by this paper
• Ultrafast Reorientational Dynamics of Leucine at the Air-Water Interface.

  2016cited by this paper
• Materials science: Improving the image of nanoparticles

  2016cited by this paper
• Second Harmonic and Sum-Frequency Generation from Aqueous Interfaces Is Modulated by Interference

  2016cited by this paper
• Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as Stabilizing Ligand and Fluorescence Signal Unit.

  2016cited by this paper
• Simultaneous characterization of protein-material and cell-protein interactions using dynamic QCM-D analysis on SAM surfaces.

  2016cited by this paper
• Investigating the Interaction of Fe Nanoparticles with Lysozyme by Biophysical and Molecular Docking Studies

  2016cited by this paper
• A multi‐resolution model to capture both global fluctuations of an enzyme and molecular recognition in the ligand‐binding site

  2016cited by this paper
• Zwitterionic ceramics for biomedical applications.

  2016cited by this paper
• Pre-coating with protein fractions inhibits nano-carrier aggregation in human blood plasma

  2016cited by this paper
• Die Steuerung des Stealth‐Effekts von Nanoträgern durch das Verständnis der Proteinkorona

  2016cited by this paper
• Diamond Quantum Devices in Biology.

  2016cited by this paper
• Stability of a Split Streptomycin Binding Aptamer.

  2016cited by this paper
• Candle soot-based super-amphiphobic coatings resist protein adsorption.

  2016cited by this paper
• Algorithms for Warping of 2-D PAGE Maps.

  2016cited by this paper
• Probing protein adsorption on a nanoparticle surface using second harmonic light scattering.

  2016cited by this paper
• Ice-nucleating bacteria control the order and dynamics of interfacial water

  2016cited by this paper
• Small Surfactant Concentration Differences Influence Adsorption of Human Serum Albumin on Polystyrene Nanoparticles.

  2016cited by this paper
• Label-free quantification in ion mobility–enhanced data-independent acquisition proteomics

  2016cited by this paper
• Fluorescence labels may significantly affect the protein adsorption on hydrophilic nanomaterials.

  2016cited by this paper
• The Nature of a Hard Protein Corona Forming on Quantum Dots Exposed to Human Blood Serum.

  2016cited by this paper
• The Intracellular Destiny of the Protein Corona: A Study on its Cellular Internalization and Evolution.

  2016cited by this paper
• Engineering and Characterization of Peptides and Proteins at Surfaces and Interfaces: A Case Study in Surface-Sensitive Vibrational Spectroscopy.

  2016cited by this paper
• Fluorescent Nanodiamond-Gold Hybrid Particles for Multimodal Optical and Electron Microscopy Cellular Imaging.

  2016cited by this paper
• Cancer nanomedicine: progress, challenges and opportunities

  2016cited by this paper
• Diamant‐Quantensensoren in der Biologie

  2016cited by this paper
• Optical label-free and model-free probe of the surface potential of nanoscale and microscopic objects in aqueous solution

  2016cited by this paper
• Protein adsorption is required for stealth effect of poly(ethylene glycol)- and poly(phosphoester)-coated nanocarriers.

  2016cited by this paper
• Controlling the Stealth Effect of Nanocarriers through Understanding the Protein Corona.

  2016cited by this paper
• Protein source and choice of anticoagulant decisively affect nanoparticle protein corona and cellular uptake.

  2016cited by this paper
• Pitfalls and novel applications of particle sizing by dynamic light scattering.

  2016cited by this paper
• Colloidally Stable and Surfactant-Free Protein-Coated Gold Nanorods in Biological Media

  2015cited by this paper
• IM30 triggers membrane fusion in cyanobacteria and chloroplasts

  2015cited by this paper
• Full membrane spanning self-assembled monolayers as model systems for UHV-based studies of cell-penetrating peptides.

  2015cited by this paper
• Folding and insertion thermodynamics of the transmembrane WALP peptide.

  2015cited by this paper
• Photocleavable linker for the patterning of bioactive molecules

  2015cited by this paper
• Kohlenhydrat‐basierte Nanocarrier mit spezifischem Zell‐Targeting und minimalem Einfluss durch die Proteinkorona

  2015cited by this paper
• Protein corona of nanoparticles: distinct proteins regulate the cellular uptake.

  2015cited by this paper
• DNA-Based Self-Assembly of Fluorescent Nanodiamonds.

  2015cited by this paper
• Programmable Biopolymers for Advancing Biomedical Applications of Fluorescent Nanodiamonds

  2015cited by this paper
• Protein adsorption onto nanoparticles induces conformational changes: Particle size dependency, kinetics, and mechanisms

  2015cited by this paper
• Mussel adhesive protein provides cohesive matrix for collagen type-1α.

  2015cited by this paper
• In situ QCM-D study of nano-bio interfaces with enhanced biocompatibility

  2015cited by this paper
• Carbohydrate-Based Nanocarriers Exhibiting Specific Cell Targeting with Minimum Influence from the Protein Corona.

  2015cited by this paper
• Competitive protein adsorption to soft polymeric layers: binary mixtures and comparison to theory.

  2015cited by this paper
• Complementary analysis of the hard and soft protein corona: sample preparation critically effects corona composition.

  2015cited by this paper
• Photon Upconversion Lithography: Patterning of Biomaterials Using Near‐Infrared Light

  2015cited by this paper
• State-of-the-art high-speed atomic force microscopy for investigation of single-molecular dynamics of proteins.

  2014cited by this paper
• Biological macromolecules at interfaces probed by chiral vibrational sum frequency generation spectroscopy.

  2014cited by this paper
• Visualization of charge propagation along individual pili proteins using ambient electrostatic force microscopy.

  2014cited by this paper
• Surface Analysis and Techniques in Biology

  2014cited by this paper
• Wechselwirkungen von Proteinen mit Polymerbeschichtungen und Biomaterialien

  2014cited by this paper
• Protein interactions with polymer coatings and biomaterials.

  2014cited by this paper
• Quantitative study of protein coronas on gold nanoparticles with different surface modifications

  2014cited by this paper
• Ex situ evaluation of the composition of protein corona of intravenously injected superparamagnetic nanoparticles in rats.

  2014cited by this paper
• The debate on the dependence of apparent contact angles on drop contact area or three-phase contact line: A review

  2014cited by this paper
• Applications of XPS in Biology and Biointerface Analysis

  2014cited by this paper
• Aggregation Behavior of Polystyrene-Nanoparticles in Human Blood Serum and its Impact on the in vivo Distribution in Mice

  2014cited by this paper
• Mass spectrometry and imaging analysis of nanoparticle-containing vesicles provide a mechanistic insight into cellular trafficking.

  2014cited by this paper
• Size-dependent knockdown potential of siRNA-loaded cationic nanohydrogel particles.

  2014cited by this paper
• Dreidimensionale Proteinnetzwerke durch Zwei‐Photonen‐ Aktivierung

  2014cited by this paper
• Application of isothermal titration calorimetry for characterizing thermodynamic parameters of biomolecular interactions: peptide self-assembly and protein adsorption case studies.

  2014cited by this paper
• Different interaction modes of biomolecules with citrate-capped gold nanoparticles.

  2014cited by this paper
• Pharmacokinetics on a microscale: visualizing Cy5-labeled oligonucleotide release from poly(n-butylcyanoacrylate) nanocapsules in cells

  2014cited by this paper
• Second harmonic light scattering from the surface of colloidal objects: theory and applications.

  2014cited by this paper
• Biomolecular structure at solid-liquid interfaces as revealed by nonlinear optical spectroscopy.

  2014cited by this paper
• Nanoparticle–Cell Interactions: Molecular Structure of the Protein Corona and Cellular Outcomes

  2014cited by this paper
• Rapid synthesis of highly luminescent and stable Au20 nanoclusters for active tumor-targeted imaging in vitro and in vivo.

  2014cited by this paper
• Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study

  2014cited by this paper
• Vibrational Sum-Frequency Scattering for Detailed Studies of Collagen Fibers in Aqueous Environments

  2014cited by this paper
• Functional superhydrophobic surfaces made of Janus micropillars

  2014cited by this paper
• Polymers in focus: fluorescence correlation spectroscopy

  2014cited by this paper
• Photopatterned antibodies for selective cell attachment.

  2014cited by this paper
• Three-dimensional protein networks assembled by two-photon activation.

  2014cited by this paper
• Interaction of Human Plasma Proteins with Thin Gelatin-Based Hydrogel Films: A QCM-D and ToF-SIMS Study

  2014cited by this paper
• Nanopatterns of polymer brushes for understanding protein adsorption on the nanoscale

  2014cited by this paper
• Polymer patchy colloids with sticky patches

  2014cited by this paper
• Accurate determination of interfacial protein secondary structure by combining interfacial-sensitive amide I and amide III spectral signals.

  2014cited by this paper
• Quantitative profiling of the protein coronas that form around nanoparticles

  2014cited by this paper
• Conjugation of Gold Nanorods with Bovine Serum Albumin Protein

  2014cited by this paper
• Protein corona formation for nanomaterials and proteins of a similar size: hard or soft corona?

  2013cited by this paper
• Supplementary Figure S4

  2013cited by this paper
• Antibacterial and hemocompatibility switchable polypropylene nonwoven fabric membrane surface.

  2013cited by this paper
• SFG analysis of surface bound proteins: a route towards structure determination.

  2013cited by this paper

• Functional and Interfacial Responses of Honeybee Pupa Water-Soluble Proteins to pH, Ionic Strength, and Sucrose

  2026cites this paper
• Site-Specific Assessment of Interfacial Processes and Dynamics via Linear and Nonlinear Infrared Techniques.

  2025cites this paper
• AI-Enhanced Surface Functionalization in Biosensors: From Fundamentals to Future Prospects

  2025cites this paper
• Impact of Glycosylation of Apolipoprotein D on Its Interaction with Gold Nanoparticles: Insights from Molecular Dynamics Simulations.

  2025cites this paper
• Unravelling drug delivery using in vitro Fluorescence Correlation Spectroscopy (FCS).

  2025cites this paper
• Shear-induced rotation enhances protein adsorption.

  2025cites this paper
• Enteral Route Nanomedicine for Cancer Therapy

  2024cites this paper
• Specific aspects of human immunoglobulin interactions with Fe3O4/≡Si(CH2)3NH2 nanocomposite surface

  2023cites this paper
• Modulating the toxicity of engineered nanoparticles by controlling protein corona formation: Recent advances and future prospects.

  2023cites this paper
• Kinetics and Timescales in Bio–Nano Interactions

  2023cites this paper
• Label-Free Quantification of Digital Nanorods Assembled from Discrete Oligourethane Amphiphiles.

  2023cites this paper
• Tracking of Protein Adsorption on Poly(l-lactic acid) Film Surfaces: The Role of Molar Mass.

  2023cites this paper
• Characterization of the Protein Corona of Three Chairside Hemoderivatives on Melt Electrowritten Polycaprolactone Scaffolds

  2023cites this paper
• Shining Light on Polymeric Drug Nanocarriers with Fluorescence Correlation Spectroscopy.

  2022cites this paper
• Digital micelles of encoded polymeric amphiphiles for direct sequence reading and ex vivo label-free quantification

  2022cites this paper
• Protein Desorption Kinetics Depends on the Timescale of Observation.

  2022cites this paper
• Patterned Carboxymethyl-Dextran Functionalized Surfaces Using Organic Mixed Monolayers for Biosensing Applications

  2022cites this paper
• Fluorescence Correlation Spectroscopy Monitors the Fate of Degradable Nanocarriers in the Blood Stream

  2022cites this paper
• Surface characteristics and osteocompatibility of titanium preserved in a ZnS@BSA-containing storage solution

  2021cites this paper
• Insights into colloidal nanoparticle-protein corona interactions for nanomedicine applications.

  2021cites this paper
• Bio-nano interactions: binding proteins, polysaccharides, lipids and nucleic acids onto magnetic nanoparticles

  2021cites this paper
• Size‐Confined Effects of Nanostructures on Fibronectin‐Induced Macrophage Inflammation on Titanium Implants

  2021cites this paper
• Interplay of interactions between micelles and fibrils of casein proteins

  2021cites this paper
• In Situ Label-Free Study of Protein Adsorption on Nanoparticles

  2021cites this paper
• Membrane Structure of Aquaporin Observed with Combined Experimental and Theoretical Sum Frequency Generation Spectroscopy.

  2021cites this paper
• A novel approach to calculate protein adsorption isotherms by molecular dynamics simulations.

  2020cites this paper
• Calcium-dependent and -independent annexin V binding: distinct molecular behaviours at cell membrane interfaces.

  2020cites this paper
• Location and Conformation of the LKα14 Peptide in Water/Ethanol Mixtures

  2020cites this paper
• Surface engineering of personal protective equipments (PPEs) to prevent the contagious infections of SARS-CoV-2

  2020cites this paper
• Effects of alloy elements on adsorption of fibrinogen on biodegradable magnesium alloys surfaces: The MD simulations and experimental studies

  2020cites this paper
• Isothermal titration calorimetry as a complementary method for investigating nanoparticle-protein interactions.

  2019cites this paper
• Covalently Binding of Bovine Serum Albumin to Unsaturated Poly(Globalide-Co-ε-Caprolactone) Nanoparticles by Thiol-Ene Reactions.

  2019cites this paper
• Möglichkeiten und Limitierungen verschiedener Trenntechniken zur Analyse der Proteinkorona

  2019cites this paper
• The quest for blood-compatible materials: Recent advances and future technologies

  2019cites this paper
• Adsorption of amino acids on graphene: assessment of current force fields.

  2019cites this paper
• Water-dispersed semiconductor nanoplatelets with high fluorescence brightness, chemical and colloidal stability.

  2019cites this paper
• Protein Adsorption Measurements on Low Fouling and Ultralow Fouling Surfaces: A Critical Comparison of Surface Characterization Techniques.

  2019cites this paper
• Relaxing the aquaporin crystal structure in a membrane with surface vibrational spectroscopy.

  2019cites this paper
• Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins.

  2019cites this paper
• Possibilities and Limitations of Different Separation Techniques for the Analysis of the Protein Corona.

  2019cites this paper
• Membrane-Protein-Hydration Interaction of α-Synuclein with Anionic Vesicles Probed via Angle-Resolved Second-Harmonic Scattering.

  2019cites this paper
• Simulations of interfacial processes: recent advances in force field development

  2019cites this paper
• The Impact of Recent Developments in Technologies which Enable the Increased Use of Biocatalysts

  2019cites this paper
• Interfacial Conformation of Hydrophilic Polyphosphoesters Affects Blood Protein Adsorption.

  2018cites this paper
• Engineering Proteins at Interfaces: From Complementary Characterization to Material Surfaces with Designed Functions

  2018cites this paper